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The map label for this gene is wcaL [H]

Identifier: 110678430

GI number: 110678430

Start: 1043614

End: 1044861

Strand: Reverse

Name: wcaL [H]

Synonym: RD1_1094

Alternate gene names: 110678430

Gene position: 1044861-1043614 (Counterclockwise)

Preceding gene: 110678431

Following gene: 110678429

Centisome position: 25.28

GC content: 61.7

Gene sequence:

>1248_bases
ATGACAACGACGCCCGCACGGCTTGCCGTTCTGGTCAAGGGATGGCCACGCCTGTCCGAGACCTTCATTGCGCAGGAACT
TGTCGCGCTGGAAGACGCAGGGCTGACGCTCGAACTCTGGTCCTTGCGCCATCCGACCGATGTGAAAACGCATCCGCTGC
ATGACCGGCTGAAGGCGCGGGTGAATTACCTGCCCGAATACCTTGAGGATGATCCGCCGCGTGTCCGGCGCGCGCTGTTC
AGCGCCAGACGCCTGCCCGGTTTCAAAGACGCCGTGCGCTGCTTTCGTGCGGATTATGCGCGCGATCAAACCGAAAACCG
CATCCGCAGGTTTGGTCAGGCCTGCGTCTTGGCGCGCGAATTACCGCCGGGTGTGACCGGCCTCTATGCGCATTTTCTGC
ACACACCGTCGTCGGTCGCGCGCTATGCCGCGATCCTGACCGGGTTGCCGTGGTCTTTCTCCGCCCACGCCAAGGACATC
TGGACCTCACCGGACTGGGAATTGCGCGAAAAACTATCAGCCCAGACATTCGGGGCGCAGTTCGGGGCCACCTGCACCGC
CTTTGGTGCGGAGCATTTGCGCAGCCTTGCAGACAGGACCGACCGCGTTGATCTGGTCTATCACGGGCTGGATCTGACGC
GCTTTCCTGACGCGCCGCACCGCAAGGCGCGCGCACCGGATGCACCACTGGCCCTTATGTCGGTGGGTCGGCTGGTGGAG
AAAAAGGGCTTTGATCGGCTGATCGAGGCGCTCGCCCTTTTGCCGGATACACTTCGTTGGCACTGGACGCATATCGGCGG
CGGTACACTCAAGCACCAGATGCAGGATCTGGCCGCTGCAAAAGGCGTGGCAGACCGTATCACATGGCGCGGCGCCTGCG
ATCAGCCCGAGGTTATCGCAGAAATGCGCACATCCGACCTGTTCGTTTTGCCCAGTCGCATTGCGGCAGATGGCGACCGC
GACGGCCTGCCCAATGTCTTGATGGAAGCCGCCAGTCAAAAGCTGCCCATTCTGGCGACCCCTGTTTCTGCCATTCCGGA
ATTCATTGACACGGGCGTGCATGGCATTCTGGTGGATGATGCCCCGGACAGCCTTGCGCAGGCGATCCGGGATTTTGCCG
CGCGGCAAGATGGCGGCCGTGCCTTTGCCGATGCGGCTTACAAAAGGCTGAAAACCGAGTTTCAGATGCAGCCGGGCATC
AATCAATTGACCAGACGGTTGCGCACGATGATGCAGGACGCGGCGTGA

Upstream 100 bases:

>100_bases
GGCTGCCAACACAACAAAGGCCATCGCAGGCGGGTGCCGAAGGCTTGCTGGATGGTCTGGACGTCGTTGTTGCACGAACC
AAAGCGTTGATGGCACAGAA

Downstream 100 bases:

>100_bases
TACCCGCCGCCTTTTACGCACCGATGAAACCACCTGATCACCCTGTGCCCTCAGGGGATCGCGCCATGGCCCGCGCCTTG
CTGACCGCATTGCAGATGTG

Product: glycosyl transferase, putative

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MTTTPARLAVLVKGWPRLSETFIAQELVALEDAGLTLELWSLRHPTDVKTHPLHDRLKARVNYLPEYLEDDPPRVRRALF
SARRLPGFKDAVRCFRADYARDQTENRIRRFGQACVLARELPPGVTGLYAHFLHTPSSVARYAAILTGLPWSFSAHAKDI
WTSPDWELREKLSAQTFGAQFGATCTAFGAEHLRSLADRTDRVDLVYHGLDLTRFPDAPHRKARAPDAPLALMSVGRLVE
KKGFDRLIEALALLPDTLRWHWTHIGGGTLKHQMQDLAAAKGVADRITWRGACDQPEVIAEMRTSDLFVLPSRIAADGDR
DGLPNVLMEAASQKLPILATPVSAIPEFIDTGVHGILVDDAPDSLAQAIRDFAARQDGGRAFADAAYKRLKTEFQMQPGI
NQLTRRLRTMMQDAA

Sequences:

>Translated_415_residues
MTTTPARLAVLVKGWPRLSETFIAQELVALEDAGLTLELWSLRHPTDVKTHPLHDRLKARVNYLPEYLEDDPPRVRRALF
SARRLPGFKDAVRCFRADYARDQTENRIRRFGQACVLARELPPGVTGLYAHFLHTPSSVARYAAILTGLPWSFSAHAKDI
WTSPDWELREKLSAQTFGAQFGATCTAFGAEHLRSLADRTDRVDLVYHGLDLTRFPDAPHRKARAPDAPLALMSVGRLVE
KKGFDRLIEALALLPDTLRWHWTHIGGGTLKHQMQDLAAAKGVADRITWRGACDQPEVIAEMRTSDLFVLPSRIAADGDR
DGLPNVLMEAASQKLPILATPVSAIPEFIDTGVHGILVDDAPDSLAQAIRDFAARQDGGRAFADAAYKRLKTEFQMQPGI
NQLTRRLRTMMQDAA
>Mature_414_residues
TTTPARLAVLVKGWPRLSETFIAQELVALEDAGLTLELWSLRHPTDVKTHPLHDRLKARVNYLPEYLEDDPPRVRRALFS
ARRLPGFKDAVRCFRADYARDQTENRIRRFGQACVLARELPPGVTGLYAHFLHTPSSVARYAAILTGLPWSFSAHAKDIW
TSPDWELREKLSAQTFGAQFGATCTAFGAEHLRSLADRTDRVDLVYHGLDLTRFPDAPHRKARAPDAPLALMSVGRLVEK
KGFDRLIEALALLPDTLRWHWTHIGGGTLKHQMQDLAAAKGVADRITWRGACDQPEVIAEMRTSDLFVLPSRIAADGDRD
GLPNVLMEAASQKLPILATPVSAIPEFIDTGVHGILVDDAPDSLAQAIRDFAARQDGGRAFADAAYKRLKTEFQMQPGIN
QLTRRLRTMMQDAA

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1788357, Length=367, Percent_Identity=25.6130790190736, Blast_Score=89, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 46247; Mature: 46116

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTPARLAVLVKGWPRLSETFIAQELVALEDAGLTLELWSLRHPTDVKTHPLHDRLKAR
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHH
VNYLPEYLEDDPPRVRRALFSARRLPGFKDAVRCFRADYARDQTENRIRRFGQACVLARE
HHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPPGVTGLYAHFLHTPSSVARYAAILTGLPWSFSAHAKDIWTSPDWELREKLSAQTFGAQ
CCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHH
FGATCTAFGAEHLRSLADRTDRVDLVYHGLDLTRFPDAPHRKARAPDAPLALMSVGRLVE
HCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
KKGFDRLIEALALLPDTLRWHWTHIGGGTLKHQMQDLAAAKGVADRITWRGACDQPEVIA
HCCHHHHHHHHHHCCHHHHEEEEECCCHHHHHHHHHHHHHHCHHHHCEECCCCCCHHHHH
EMRTSDLFVLPSRIAADGDRDGLPNVLMEAASQKLPILATPVSAIPEFIDTGVHGILVDD
HHHCCCEEEECHHHHCCCCCCCCHHHHHHHHHCCCCEEECCHHHHHHHHHCCCCEEEECC
APDSLAQAIRDFAARQDGGRAFADAAYKRLKTEFQMQPGINQLTRRLRTMMQDAA
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTTPARLAVLVKGWPRLSETFIAQELVALEDAGLTLELWSLRHPTDVKTHPLHDRLKAR
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHH
VNYLPEYLEDDPPRVRRALFSARRLPGFKDAVRCFRADYARDQTENRIRRFGQACVLARE
HHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPPGVTGLYAHFLHTPSSVARYAAILTGLPWSFSAHAKDIWTSPDWELREKLSAQTFGAQ
CCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHH
FGATCTAFGAEHLRSLADRTDRVDLVYHGLDLTRFPDAPHRKARAPDAPLALMSVGRLVE
HCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
KKGFDRLIEALALLPDTLRWHWTHIGGGTLKHQMQDLAAAKGVADRITWRGACDQPEVIA
HCCHHHHHHHHHHCCHHHHEEEEECCCHHHHHHHHHHHHHHCHHHHCEECCCCCCHHHHH
EMRTSDLFVLPSRIAADGDRDGLPNVLMEAASQKLPILATPVSAIPEFIDTGVHGILVDD
HHHCCCEEEECHHHHCCCCCCCCHHHHHHHHHCCCCEEECCHHHHHHHHHCCCCEEEECC
APDSLAQAIRDFAARQDGGRAFADAAYKRLKTEFQMQPGINQLTRRLRTMMQDAA
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]