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The map label for this gene is yiaO [H]

Identifier: 110678393

GI number: 110678393

Start: 1013751

End: 1014728

Strand: Reverse

Name: yiaO [H]

Synonym: RD1_1052

Alternate gene names: 110678393

Gene position: 1014728-1013751 (Counterclockwise)

Preceding gene: 110678394

Following gene: 110678392

Centisome position: 24.55

GC content: 57.67

Gene sequence:

>978_bases
ATGAGGCTCTTTACAAAAATCAAAGGACTGGCTGCGGTGACCTGCGTTGCAGCGCTCGCGTCTTCAGCCGCATTTGCGCA
GGAGATGACCCTGAAACTGGGGCATCTGGCAAATGAGCAAAACGCATGGCACCTCGCAGCGGTCAAATTCGGCGAGGAAC
TCTCCACGCTGACCGACGGTCGCATTGCCGTCGAGGTTTTCCCGAACGAATCCCTCGGCAAGGAAATCGACCTGATCAAC
GGCATGCAACTTGGCACGGTGGACATGACGATCACCGGCGAAAGCCTGCAAAACTGGGCCCCGATGGCGGCGTTGCTGGC
CGTACCCTATGCCTACAAATCCCTTGAGCATATGGACGAAGTCGCCTCTGGCGAAATCGGTGAACAGATCAAACAGCAGA
TCATCGAAAAGGCCCAAGTGCGCCCGATTGCGTTCTTTGCCCGTGGGCCGCGCAATCTGACGTCGCAACGCCCGATCACA
TCACCTGCAGACCTTGATGGCATGAAAATGCGCGTCCCGAACGTGCCGCTTTTCGTGGACGTCTGGAGCGCTCTGGGCGC
ATCGCCAACACCGATGGCATTCTCCGAGGTCTTTACCTCCCTGCAAAACGGCGTGATCGACGGTCAGGAAAACCCGCTTG
CCCTGATCCGCTCGGCCAACTTCAATGAAGTCCAGGGCTATGTGAACCAGACCGAACATGTCCGGTCCTGGATTTACCTG
ACAATCGCGGAATCCACCTGGGCCAAACTGTCCGAGGACGATCAGAACGCCGTCATGCAGGCCGCAGCAACAGCGCAGGA
ATATGAGCGTGGTCTGCTGCTGGAAAGCCTCGCCGAAGATCGCGGCTATCTCGAAAGCAAGGGCATGACCTTCGTCGAGG
TGGATGGCGCGGCGTTTCAGGCCGCTGCCAAGGACGCGGTTCTTGCCAACGTAAGCGAAGAAATCCGCCCCATCGTGGAA
TCGCTGTTCTCTGAATAA

Upstream 100 bases:

>100_bases
CTAAGCTCGCTCCCAAAGGTGCAGGCCCTTTACCCCGCGTATTTCGACGGGCCTGCACAAACAATATCCGATCCCAAGCC
TTAACACTGGAGGAAACATC

Downstream 100 bases:

>100_bases
AACGCCCGCGAGAGCCGTCGGTCATCGACCCGGCGGCTCTTGCACCTATTTTAATGAAAGTCTGACGGATGCAGCGGATA
GAACGGATCATCGTGGGTTT

Product: C4-dicarboxylate transport system, substrate-binding protein, putative

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MRLFTKIKGLAAVTCVAALASSAAFAQEMTLKLGHLANEQNAWHLAAVKFGEELSTLTDGRIAVEVFPNESLGKEIDLIN
GMQLGTVDMTITGESLQNWAPMAALLAVPYAYKSLEHMDEVASGEIGEQIKQQIIEKAQVRPIAFFARGPRNLTSQRPIT
SPADLDGMKMRVPNVPLFVDVWSALGASPTPMAFSEVFTSLQNGVIDGQENPLALIRSANFNEVQGYVNQTEHVRSWIYL
TIAESTWAKLSEDDQNAVMQAAATAQEYERGLLLESLAEDRGYLESKGMTFVEVDGAAFQAAAKDAVLANVSEEIRPIVE
SLFSE

Sequences:

>Translated_325_residues
MRLFTKIKGLAAVTCVAALASSAAFAQEMTLKLGHLANEQNAWHLAAVKFGEELSTLTDGRIAVEVFPNESLGKEIDLIN
GMQLGTVDMTITGESLQNWAPMAALLAVPYAYKSLEHMDEVASGEIGEQIKQQIIEKAQVRPIAFFARGPRNLTSQRPIT
SPADLDGMKMRVPNVPLFVDVWSALGASPTPMAFSEVFTSLQNGVIDGQENPLALIRSANFNEVQGYVNQTEHVRSWIYL
TIAESTWAKLSEDDQNAVMQAAATAQEYERGLLLESLAEDRGYLESKGMTFVEVDGAAFQAAAKDAVLANVSEEIRPIVE
SLFSE
>Mature_325_residues
MRLFTKIKGLAAVTCVAALASSAAFAQEMTLKLGHLANEQNAWHLAAVKFGEELSTLTDGRIAVEVFPNESLGKEIDLIN
GMQLGTVDMTITGESLQNWAPMAALLAVPYAYKSLEHMDEVASGEIGEQIKQQIIEKAQVRPIAFFARGPRNLTSQRPIT
SPADLDGMKMRVPNVPLFVDVWSALGASPTPMAFSEVFTSLQNGVIDGQENPLALIRSANFNEVQGYVNQTEHVRSWIYL
TIAESTWAKLSEDDQNAVMQAAATAQEYERGLLLESLAEDRGYLESKGMTFVEVDGAAFQAAAKDAVLANVSEEIRPIVE
SLFSE

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=298, Percent_Identity=30.8724832214765, Blast_Score=166, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 35384; Mature: 35384

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFTKIKGLAAVTCVAALASSAAFAQEMTLKLGHLANEQNAWHLAAVKFGEELSTLTDG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHCCC
RIAVEVFPNESLGKEIDLINGMQLGTVDMTITGESLQNWAPMAALLAVPYAYKSLEHMDE
EEEEEECCCCCCCCCHHHHCCCEECEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
VASGEIGEQIKQQIIEKAQVRPIAFFARGPRNLTSQRPITSPADLDGMKMRVPNVPLFVD
HHCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCHHEE
VWSALGASPTPMAFSEVFTSLQNGVIDGQENPLALIRSANFNEVQGYVNQTEHVRSWIYL
HHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHEEEEE
TIAESTWAKLSEDDQNAVMQAAATAQEYERGLLLESLAEDRGYLESKGMTFVEVDGAAFQ
EEECCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEECCHHHH
AAAKDAVLANVSEEIRPIVESLFSE
HHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRLFTKIKGLAAVTCVAALASSAAFAQEMTLKLGHLANEQNAWHLAAVKFGEELSTLTDG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHCCC
RIAVEVFPNESLGKEIDLINGMQLGTVDMTITGESLQNWAPMAALLAVPYAYKSLEHMDE
EEEEEECCCCCCCCCHHHHCCCEECEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
VASGEIGEQIKQQIIEKAQVRPIAFFARGPRNLTSQRPITSPADLDGMKMRVPNVPLFVD
HHCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCHHEE
VWSALGASPTPMAFSEVFTSLQNGVIDGQENPLALIRSANFNEVQGYVNQTEHVRSWIYL
HHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHEEEEE
TIAESTWAKLSEDDQNAVMQAAATAQEYERGLLLESLAEDRGYLESKGMTFVEVDGAAFQ
EEECCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEECCHHHH
AAAKDAVLANVSEEIRPIVESLFSE
HHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]