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The map label for this gene is mscL

Identifier: 110678359

GI number: 110678359

Start: 977823

End: 978251

Strand: Direct

Name: mscL

Synonym: RD1_1015

Alternate gene names: 110678359

Gene position: 977823-978251 (Clockwise)

Preceding gene: 110678358

Following gene: 110678362

Centisome position: 23.66

GC content: 51.05

Gene sequence:

>429_bases
ATGCTCAACGAATTTAAGACCTTTATTTCAAAGGGAAATGTCATGGATATGGCGGTCGGTATCATCATTGGTGCCGCATT
TACCGCCATCGTTTCATCTCTGGTCGCAGACCTTGTGAACCCGTTTATCGCGCTTTTCACCGGTGGCATCGACTTTTCGG
GGTGGTTTTATGCGCTTGACGGCGAAACATATGCGTCACTTGCAGCGGCAACCGATGCGGGTGCGCCGGTCTTTGCCTTT
GGCAATTTCATCATGGCCGTGATCAATTTTCTGATCATCGCCTTTGTGGTTTTTATGCTGGTCAAAACGGTGAACCGCAT
CAAAGACGCCGCCGAAGGTGAAAAAGAGGCTGTTGCCGAAGAACCCGCAGGCCCGACCGAGTTGGATATTCTCAAGGAAA
TCCGCGACGCCTTGGCCAAACAAGGCTGA

Upstream 100 bases:

>100_bases
GTGTTTTTCCCCCGTGGATGCGCCTTACAACACGGCTCTTATGTGTTAACGAAAGGTTAACGGCCATGTTCAGGGCCGTC
ATCAACAGTACGAGGGAAAA

Downstream 100 bases:

>100_bases
TCGCTTCTGAAGCGTCATGCAAAAAACCTCGATCACGCAACGCTGCCTGAAACCCGGGGTTTCAACGCGTTGCGCGATGC
TTGATGTTCCAGGTATTTCG

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 142; Mature: 142

Protein sequence:

>142_residues
MLNEFKTFISKGNVMDMAVGIIIGAAFTAIVSSLVADLVNPFIALFTGGIDFSGWFYALDGETYASLAAATDAGAPVFAF
GNFIMAVINFLIIAFVVFMLVKTVNRIKDAAEGEKEAVAEEPAGPTELDILKEIRDALAKQG

Sequences:

>Translated_142_residues
MLNEFKTFISKGNVMDMAVGIIIGAAFTAIVSSLVADLVNPFIALFTGGIDFSGWFYALDGETYASLAAATDAGAPVFAF
GNFIMAVINFLIIAFVVFMLVKTVNRIKDAAEGEKEAVAEEPAGPTELDILKEIRDALAKQG
>Mature_142_residues
MLNEFKTFISKGNVMDMAVGIIIGAAFTAIVSSLVADLVNPFIALFTGGIDFSGWFYALDGETYASLAAATDAGAPVFAF
GNFIMAVINFLIIAFVVFMLVKTVNRIKDAAEGEKEAVAEEPAGPTELDILKEIRDALAKQG

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=142, Percent_Identity=43.6619718309859, Blast_Score=103, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_ROSDO (Q16BG3)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_681366.1
- ProteinModelPortal:   Q16BG3
- SMR:   Q16BG3
- STRING:   Q16BG3
- GeneID:   4198261
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_1015
- NMPDR:   fig|375451.6.peg.952
- eggNOG:   COG1970
- HOGENOM:   HBG727604
- OMA:   GANVLAW
- PhylomeDB:   Q16BG3
- ProtClustDB:   PRK13952
- BioCyc:   RDEN375451:RD1_1015-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 15075; Mature: 15075

Theoretical pI: Translated: 4.08; Mature: 4.08

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x12d26c68)-; HASH(0x1d3a5d24)-; HASH(0x1d4c5a98)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNEFKTFISKGNVMDMAVGIIIGAAFTAIVSSLVADLVNPFIALFTGGIDFSGWFYALD
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEC
GETYASLAAATDAGAPVFAFGNFIMAVINFLIIAFVVFMLVKTVNRIKDAAEGEKEAVAE
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHC
EPAGPTELDILKEIRDALAKQG
CCCCCHHHHHHHHHHHHHHCCH
>Mature Secondary Structure
MLNEFKTFISKGNVMDMAVGIIIGAAFTAIVSSLVADLVNPFIALFTGGIDFSGWFYALD
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEC
GETYASLAAATDAGAPVFAFGNFIMAVINFLIIAFVVFMLVKTVNRIKDAAEGEKEAVAE
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHC
EPAGPTELDILKEIRDALAKQG
CCCCCHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA