Click here to switch to the map view.

The map label for this gene is ada [H]

Identifier: 110678311

GI number: 110678311

Start: 929311

End: 930177

Strand: Direct

Name: ada [H]

Synonym: RD1_0965

Alternate gene names: 110678311

Gene position: 929311-930177 (Clockwise)

Preceding gene: 110678307

Following gene: 110678312

Centisome position: 22.48

GC content: 58.02

Gene sequence:

>867_bases
ATGACACAGCACCAGACCCTGCCACAAACCACCGAAGGCGCCTATCATTACCAAGTGATACGTCGGGCGATCGAACTGAT
TGATCAAACGGATCAGCCCATCTCCCTTGATGAGATTGCCGCAAACATGGGGATGTCCCCTGCGCATTTTCAGCGTGTAT
TCTCGCGGTGGGTGGGCGTTTCCCCGAAACGGTATCAGCAGTATCTGACCCTTGGACATGCAAAAACCCTGCTGAGTGAA
CGGTTTTCGACGCTTGCGGCATCTCATGCTGTGGGTTTGTCCGGCAGCGGGCGTTTGCATGATCTGTTCCTGCGGTGGGA
AGCGATGAGCCCGGGTGAATTCGCGCGCAAGGGGGCGGGATTGACCATTTACTGGGGCTGGTTCGAAAGCCCCTTTGGCC
CCGCGATTGTCATGGGCACGGAAAAAGGCATCTGCGGCATGGGATTTGCGGCGGAAATGGGCGCTGAAACTGCATTTGAG
GATCTTGCCGCGCGCTGGCCGCAGGCGTCCTATGTCGAAGACCCGATGATGCTGCGCCCATGGGTGTTGCAGGCGTTCGG
TGCCAAGGATGGCGCGCTGGATACCACACCGTTGTTTTTGATTGGTGCGCCGTTCCAGATAAAAGTCTGGGAAGCGCTTT
TGTCCATTCCGACGGGCCATGTCACGACCTACTCCGAAATCGCGCAAGCTGTTGGTGCGCCGCGTGCGGTGCGCGCGGTT
GGTACGGCTGTGGGCAGAAATCCGATCAGTTGGATCATTCCCTGTCACCGTGCCCTGCGTAAATCCGGCGGTCTGGGGGG
GTATCACTGGGGCATCCCCATGAAACGCGCGATGCTTGCCTATGAGGCGGTCGAACTGGAAACATAA

Upstream 100 bases:

>100_bases
GCGAACCTGCATGGCGTGCCGCAATTTTCAGTGAACAGGGGCAGGGTGTAACCGCATGAAACGGGTCGCGTGGCCCTCGA
TAACGGCGTAAACATGCGCC

Downstream 100 bases:

>100_bases
AGTTTACCGGCGGGATGTCGCCTGTCGATTTTTCCCCTTTGTTTGCCGGGCCGCGTATCCCATATATGCTGACGGACCAT
AACGCCGCCCCGCCCGAACG

Product: ADA regulatory protein, putative

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MTQHQTLPQTTEGAYHYQVIRRAIELIDQTDQPISLDEIAANMGMSPAHFQRVFSRWVGVSPKRYQQYLTLGHAKTLLSE
RFSTLAASHAVGLSGSGRLHDLFLRWEAMSPGEFARKGAGLTIYWGWFESPFGPAIVMGTEKGICGMGFAAEMGAETAFE
DLAARWPQASYVEDPMMLRPWVLQAFGAKDGALDTTPLFLIGAPFQIKVWEALLSIPTGHVTTYSEIAQAVGAPRAVRAV
GTAVGRNPISWIIPCHRALRKSGGLGGYHWGIPMKRAMLAYEAVELET

Sequences:

>Translated_288_residues
MTQHQTLPQTTEGAYHYQVIRRAIELIDQTDQPISLDEIAANMGMSPAHFQRVFSRWVGVSPKRYQQYLTLGHAKTLLSE
RFSTLAASHAVGLSGSGRLHDLFLRWEAMSPGEFARKGAGLTIYWGWFESPFGPAIVMGTEKGICGMGFAAEMGAETAFE
DLAARWPQASYVEDPMMLRPWVLQAFGAKDGALDTTPLFLIGAPFQIKVWEALLSIPTGHVTTYSEIAQAVGAPRAVRAV
GTAVGRNPISWIIPCHRALRKSGGLGGYHWGIPMKRAMLAYEAVELET
>Mature_287_residues
TQHQTLPQTTEGAYHYQVIRRAIELIDQTDQPISLDEIAANMGMSPAHFQRVFSRWVGVSPKRYQQYLTLGHAKTLLSER
FSTLAASHAVGLSGSGRLHDLFLRWEAMSPGEFARKGAGLTIYWGWFESPFGPAIVMGTEKGICGMGFAAEMGAETAFED
LAARWPQASYVEDPMMLRPWVLQAFGAKDGALDTTPLFLIGAPFQIKVWEALLSIPTGHVTTYSEIAQAVGAPRAVRAVG
TAVGRNPISWIIPCHRALRKSGGLGGYHWGIPMKRAMLAYEAVELET

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=169, Percent_Identity=30.7692307692308, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1788542, Length=284, Percent_Identity=29.9295774647887, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787596, Length=110, Percent_Identity=39.0909090909091, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI115533068, Length=128, Percent_Identity=34.375, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI115533070, Length=128, Percent_Identity=34.375, Blast_Score=97, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6320001, Length=84, Percent_Identity=44.047619047619, Blast_Score=88, Evalue=1e-18,
Organism=Drosophila melanogaster, GI17137554, Length=171, Percent_Identity=30.4093567251462, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 31636; Mature: 31505

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQHQTLPQTTEGAYHYQVIRRAIELIDQTDQPISLDEIAANMGMSPAHFQRVFSRWVGV
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCC
SPKRYQQYLTLGHAKTLLSERFSTLAASHAVGLSGSGRLHDLFLRWEAMSPGEFARKGAG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCHHHHHCCCC
LTIYWGWFESPFGPAIVMGTEKGICGMGFAAEMGAETAFEDLAARWPQASYVEDPMMLRP
EEEEECCCCCCCCCEEEEECCCCCCCCCHHHHHCHHHHHHHHHHHCCCCCCCCCCHHHHH
WVLQAFGAKDGALDTTPLFLIGAPFQIKVWEALLSIPTGHVTTYSEIAQAVGAPRAVRAV
HHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHH
GTAVGRNPISWIIPCHRALRKSGGLGGYHWGIPMKRAMLAYEAVELET
HHHHCCCCCHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TQHQTLPQTTEGAYHYQVIRRAIELIDQTDQPISLDEIAANMGMSPAHFQRVFSRWVGV
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCC
SPKRYQQYLTLGHAKTLLSERFSTLAASHAVGLSGSGRLHDLFLRWEAMSPGEFARKGAG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCHHHHHCCCC
LTIYWGWFESPFGPAIVMGTEKGICGMGFAAEMGAETAFEDLAARWPQASYVEDPMMLRP
EEEEECCCCCCCCCEEEEECCCCCCCCCHHHHHCHHHHHHHHHHHCCCCCCCCCCHHHHH
WVLQAFGAKDGALDTTPLFLIGAPFQIKVWEALLSIPTGHVTTYSEIAQAVGAPRAVRAV
HHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHH
GTAVGRNPISWIIPCHRALRKSGGLGGYHWGIPMKRAMLAYEAVELET
HHHHCCCCCHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]