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The map label for this gene is nth [H]

Identifier: 110678310

GI number: 110678310

Start: 928488

End: 929234

Strand: Reverse

Name: nth [H]

Synonym: RD1_0963

Alternate gene names: 110678310

Gene position: 929234-928488 (Counterclockwise)

Preceding gene: 110678315

Following gene: 110678309

Centisome position: 22.48

GC content: 56.76

Gene sequence:

>747_bases
TTGCGGCACGCCATGCAGGTTCGCAGCAACGGTGCACGCCGGACATTCAAATCAGCGGATCATGCTCTTGCCCGTGCGCG
GGAAAGGACGCATACCACCACCATGGCAAAACAACTCGATTATCATACGATCCGGGAAATTTTTACACGCTTTCAGGCGG
CTGATCCAGAACCCAAGGGCGAGCTTGAGCATGTGAACGTCTATACGCTGGTTGTGGCTGTCGCCCTCAGCGCGCAGGCC
ACGGACGCGGGCGTCAACAAAGCCACACGTGCGCTGTTCAAGATCGCGGATACCCCGCAAAAGATGTTGGATCTGGGGCT
TGAAGGTGTCACGGAGCACATCAAGACAATCGGTCTTTTTCGTCAAAAGGCCAAGAATGTCATCAAGCTGAGCCAGATTC
TTGTTGATCAATATGGCGGCGTTGTACCCAACAGCCGCGCCGCACTGCAAAGCCTTCCGGGTGTGGGTCGCAAGACGGCC
AACGTGGTGTTGAACATGTGGTGGCAGCAGCCTGCACAGGCCGTGGACACGCATATCTTTCGGCTCGGAAACCGCACCGG
GATCGCCCCCGGCAAGACGGTCGACATCGTCGAGCGCGCCATCGAGGATAACATTCCGGCAGATTTCCAGCTTCATGCGC
ATCATTGGATGATCCTGCATGGGCGCTACCACTGCAAAGCGCGCAAGCCCCTGTGCGGGACCTGCATCATAAACGATCTC
TGCCCATTTGAGGACAAAACCGAATGA

Upstream 100 bases:

>100_bases
TGGCAGGGTCTGGTGCTGTGTCATGGCGCATGTTTACGCCGTTATCGAGGGCCACGCGACCCGTTTCATGCGGTTACACC
CTGCCCCTGTTCACTGAAAA

Downstream 100 bases:

>100_bases
CACAATACGAAGTGGTCGGCATCGGAAACGCCGTTGTCGACGTGATCAGCCATGCCGATGACAGTTTTCTCGCCCATATG
GGCATCGAAAAAGGCATCAT

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MRHAMQVRSNGARRTFKSADHALARARERTHTTTMAKQLDYHTIREIFTRFQAADPEPKGELEHVNVYTLVVAVALSAQA
TDAGVNKATRALFKIADTPQKMLDLGLEGVTEHIKTIGLFRQKAKNVIKLSQILVDQYGGVVPNSRAALQSLPGVGRKTA
NVVLNMWWQQPAQAVDTHIFRLGNRTGIAPGKTVDIVERAIEDNIPADFQLHAHHWMILHGRYHCKARKPLCGTCIINDL
CPFEDKTE

Sequences:

>Translated_248_residues
MRHAMQVRSNGARRTFKSADHALARARERTHTTTMAKQLDYHTIREIFTRFQAADPEPKGELEHVNVYTLVVAVALSAQA
TDAGVNKATRALFKIADTPQKMLDLGLEGVTEHIKTIGLFRQKAKNVIKLSQILVDQYGGVVPNSRAALQSLPGVGRKTA
NVVLNMWWQQPAQAVDTHIFRLGNRTGIAPGKTVDIVERAIEDNIPADFQLHAHHWMILHGRYHCKARKPLCGTCIINDL
CPFEDKTE
>Mature_248_residues
MRHAMQVRSNGARRTFKSADHALARARERTHTTTMAKQLDYHTIREIFTRFQAADPEPKGELEHVNVYTLVVAVALSAQA
TDAGVNKATRALFKIADTPQKMLDLGLEGVTEHIKTIGLFRQKAKNVIKLSQILVDQYGGVVPNSRAALQSLPGVGRKTA
NVVLNMWWQQPAQAVDTHIFRLGNRTGIAPGKTVDIVERAIEDNIPADFQLHAHHWMILHGRYHCKARKPLCGTCIINDL
CPFEDKTE

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=181, Percent_Identity=29.2817679558011, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI1787920, Length=203, Percent_Identity=56.1576354679803, Blast_Score=244, Evalue=5e-66,
Organism=Caenorhabditis elegans, GI17554540, Length=179, Percent_Identity=30.7262569832402, Blast_Score=97, Evalue=6e-21,
Organism=Drosophila melanogaster, GI45550361, Length=175, Percent_Identity=30.2857142857143, Blast_Score=93, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 27715; Mature: 27715

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHAMQVRSNGARRTFKSADHALARARERTHTTTMAKQLDYHTIREIFTRFQAADPEPKG
CCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ELEHVNVYTLVVAVALSAQATDAGVNKATRALFKIADTPQKMLDLGLEGVTEHIKTIGLF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCHHHHHHHHHHHHHH
RQKAKNVIKLSQILVDQYGGVVPNSRAALQSLPGVGRKTANVVLNMWWQQPAQAVDTHIF
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RLGNRTGIAPGKTVDIVERAIEDNIPADFQLHAHHWMILHGRYHCKARKPLCGTCIINDL
HHCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECEEEEEEECEEEECCCCCCHHHHHHHCC
CPFEDKTE
CCCCCCCC
>Mature Secondary Structure
MRHAMQVRSNGARRTFKSADHALARARERTHTTTMAKQLDYHTIREIFTRFQAADPEPKG
CCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ELEHVNVYTLVVAVALSAQATDAGVNKATRALFKIADTPQKMLDLGLEGVTEHIKTIGLF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCHHHHHHHHHHHHHH
RQKAKNVIKLSQILVDQYGGVVPNSRAALQSLPGVGRKTANVVLNMWWQQPAQAVDTHIF
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RLGNRTGIAPGKTVDIVERAIEDNIPADFQLHAHHWMILHGRYHCKARKPLCGTCIINDL
HHCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECEEEEEEECEEEECCCCCCHHHHHHHCC
CPFEDKTE
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]