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The map label for this gene is thiG

Identifier: 110678112

GI number: 110678112

Start: 722133

End: 722903

Strand: Direct

Name: thiG

Synonym: RD1_0749

Alternate gene names: 110678112

Gene position: 722133-722903 (Clockwise)

Preceding gene: 110678111

Following gene: 110678113

Centisome position: 17.47

GC content: 62.13

Gene sequence:

>771_bases
ATGCCCATTTTTTACGGCACAGAAGTGACATCCCGGCTGATGCTGGGCACCGCGCAATACCCGTCGCCCGCAATTTTGGC
GGATGCTTTTCGCCGCTCGGACGCAGGGATTGCCACGGTTTCAGTGCGGCGCGAGGCGGGTGGTGATCAGGCGGGGCAGG
ATTTCTGGGGGTTGATCCGGGATCTGGGTGTCGCCGTGCTTCCCAACACCGCAGGCTGTTATTCGGTGCGCGAGGCCGTG
ACCACTGCCCAGATGGCGCGCGAGTTGTTCGACACCAACTGGATCAAGCTCGAGGTCATCGGCCACACCGATACATTGCA
ACCTGATCCCTTCGGGCTCGTCGAAGCAGCCGCACAACTGGCCGAGGACGGGTTTGAGGTGTTCCCTTATTGCACCGAAG
ACTTGGTGCTGTGCGAGCGGCTGGTCGAGGTCGGTTGCAAGGTTTTGATGCCATGGGGCGCACCCATCGGCACCGGCTTG
GGGCTGAACAACATCTACGGTCTGCGCAGCTTGCGTGCGCATTTTCCCGATACGCCACTGGTCGTGGATGCAGGTCTTGG
ATTGCCCTCCCATGCGACGCAAGCGATGGAGTTGGGGTATGACGCGGTTCTGTTGAACACAGCCGTTTCAAAAGCGGGCG
ATCCGGCGGCGATGGCGCACGCCTTTGCTTTGGCGGTCGAAGCCGGCGCGCTGGCCGCCGGTGCCGATCCCATGGATGCA
CGCGACATGGCGGCGCCTTCCACGCCCGTCTTCGGAAAGGCCATGCTATGA

Upstream 100 bases:

>100_bases
TCGCTGAACGAGACATTCGTGTCCAGGCATCAGCGCGCCGAGAATTCTTTGCAAGAGGGGGATCGCGTCGAAATCGTGGC
CCCGCGACAAGGAGGATAGA

Downstream 100 bases:

>100_bases
CGCTGCCCCGCTTTTACCCGATCTTTGACGATGTGGCCTGGCTGCGGCGCATGCTGCCCTTGGGGGTGAAGTTCGTGCAG
CTGCGCATCAAAGACCAGCC

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MPIFYGTEVTSRLMLGTAQYPSPAILADAFRRSDAGIATVSVRREAGGDQAGQDFWGLIRDLGVAVLPNTAGCYSVREAV
TTAQMARELFDTNWIKLEVIGHTDTLQPDPFGLVEAAAQLAEDGFEVFPYCTEDLVLCERLVEVGCKVLMPWGAPIGTGL
GLNNIYGLRSLRAHFPDTPLVVDAGLGLPSHATQAMELGYDAVLLNTAVSKAGDPAAMAHAFALAVEAGALAAGADPMDA
RDMAAPSTPVFGKAML

Sequences:

>Translated_256_residues
MPIFYGTEVTSRLMLGTAQYPSPAILADAFRRSDAGIATVSVRREAGGDQAGQDFWGLIRDLGVAVLPNTAGCYSVREAV
TTAQMARELFDTNWIKLEVIGHTDTLQPDPFGLVEAAAQLAEDGFEVFPYCTEDLVLCERLVEVGCKVLMPWGAPIGTGL
GLNNIYGLRSLRAHFPDTPLVVDAGLGLPSHATQAMELGYDAVLLNTAVSKAGDPAAMAHAFALAVEAGALAAGADPMDA
RDMAAPSTPVFGKAML
>Mature_255_residues
PIFYGTEVTSRLMLGTAQYPSPAILADAFRRSDAGIATVSVRREAGGDQAGQDFWGLIRDLGVAVLPNTAGCYSVREAVT
TAQMARELFDTNWIKLEVIGHTDTLQPDPFGLVEAAAQLAEDGFEVFPYCTEDLVLCERLVEVGCKVLMPWGAPIGTGLG
LNNIYGLRSLRAHFPDTPLVVDAGLGLPSHATQAMELGYDAVLLNTAVSKAGDPAAMAHAFALAVEAGALAAGADPMDAR
DMAAPSTPVFGKAML

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=242, Percent_Identity=42.9752066115703, Blast_Score=183, Evalue=8e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_ROSDO (Q16C60)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_681119.1
- ProteinModelPortal:   Q16C60
- SMR:   Q16C60
- STRING:   Q16C60
- GeneID:   4197894
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_0749
- NMPDR:   fig|375451.6.peg.702
- eggNOG:   COG2022
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- PhylomeDB:   Q16C60
- ProtClustDB:   PRK00208
- BioCyc:   RDEN375451:RD1_0749-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 26940; Mature: 26809

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: NA

Important sites: ACT_SITE 96-96 BINDING 157-157

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIFYGTEVTSRLMLGTAQYPSPAILADAFRRSDAGIATVSVRREAGGDQAGQDFWGLIR
CCEEECHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEEEEHHCCCCCCCHHHHHHHH
DLGVAVLPNTAGCYSVREAVTTAQMARELFDTNWIKLEVIGHTDTLQPDPFGLVEAAAQL
HCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHH
AEDGFEVFPYCTEDLVLCERLVEVGCKVLMPWGAPIGTGLGLNNIYGLRSLRAHFPDTPL
HHCCCEECCCCHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCE
VVDAGLGLPSHATQAMELGYDAVLLNTAVSKAGDPAAMAHAFALAVEAGALAAGADPMDA
EEECCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCH
RDMAAPSTPVFGKAML
HHCCCCCCCCCCCCCC
>Mature Secondary Structure 
PIFYGTEVTSRLMLGTAQYPSPAILADAFRRSDAGIATVSVRREAGGDQAGQDFWGLIR
CEEECHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEEEEHHCCCCCCCHHHHHHHH
DLGVAVLPNTAGCYSVREAVTTAQMARELFDTNWIKLEVIGHTDTLQPDPFGLVEAAAQL
HCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHH
AEDGFEVFPYCTEDLVLCERLVEVGCKVLMPWGAPIGTGLGLNNIYGLRSLRAHFPDTPL
HHCCCEECCCCHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCE
VVDAGLGLPSHATQAMELGYDAVLLNTAVSKAGDPAAMAHAFALAVEAGALAAGADPMDA
EEECCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCH
RDMAAPSTPVFGKAML
HHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA