BacMap

Definition	Roseobacter denitrificans OCh 114, complete genome.
Accession	NC_008209
Length	4,133,097

Click here to switch to the map view.

The map label for this gene is livF [H]

Identifier: 110678083

GI number: 110678083

Start: 686729

End: 687556

Strand: Direct

Name: livF [H]

Synonym: RD1_0709

Alternate gene names: 110678083

Gene position: 686729-687556 (Clockwise)

Preceding gene: 110678082

Following gene: 110678084

Centisome position: 16.62

GC content: 56.76

Gene sequence:

>828_bases
ATGTTCGACGCAGCAGAGGGCCAGACCGAGAACGTTCTCGAAGTGAACAACATCGAGGTGATCTACAACCACGTTATTCT
CGTGCTGAAAGGCGTGAGCCTGAATGTGCCGCAGGGGGGCATCACCGCCTTGCTCGGTGGGAATGGCGCGGGCAAGACGA
CGACCCTCAAGGCGATTTCGGGCCTTTTGGCCTCTGAGCGCGGCGAGGTGACGAAAGGCTCCATCAAATATCGCGGCACC
GTCATCCAGCATCAGGACCCCGCCGATATCGTGAAATCCGGTGTCGTGCAGGTGATGGAAGGGCGGCACTGCTTTGAACA
CCTCACGGTCGAGGAAAACCTGCTGACCGGTGCCTATACGCGGCGCGAGGGCAAGGCCGCGATCCAACGTGATCTGGAGA
TGGTCTATGACTATTTCCCGCGCCTGAAAGAGCGGCGCAAAAGCCAGGCGGGCTATACCTCGGGCGGAGAGCAGCAGATG
ACTGCCATCGGGCGGGCGCTGATGTCAAAACCGGAAACGATTCTGCTCGATGAACCCTCGATGGGTCTTGCGCCGCAGCT
GGTTGAGCAAATTTTTGAGATCGTGAAATCTGTCAATGAAAACGAGGGGGTAACTTTTTTGTTGGCGGAGCAGAACACGA
ATGTGGCGCTACGCTTCGCGCATTACGGGTATATTTTGGAATCCGGACGTGTTGTGATGGATGGACCCGCAGCGGATTTG
CGGGAAAACCCGGACGTAAAGGAATTTTATCTGGGTATGTCTGAACAGGGGCGAAAGTCCTTTCGCGATGTGCGTAGTTA
CCGCCGACGCAAGCGTTGGCTCAGCTGA

Upstream 100 bases:

>100_bases
GCCGGGGAACGCACCACGAGACAGGCCGGACGTCCTGCATCCAGCGGGCGTTCAACGGGCAGACAGGGGCGGCCCGGCGC
ACAGATCAGGAGCGATGGAA

Downstream 100 bases:

>100_bases
AGGAGGAACAAAGTGCGCGGCCCCACGACAAAGCAGCCATCGCTGGCGATTGCAAAAGCCCGCTGCGGATTTGCAGCGAG
GCGTGCCGTGCAGGATATGA

Product: branched-chain amino acid ABC transporter, ATP-binding protein, putative

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein F [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MFDAAEGQTENVLEVNNIEVIYNHVILVLKGVSLNVPQGGITALLGGNGAGKTTTLKAISGLLASERGEVTKGSIKYRGT
VIQHQDPADIVKSGVVQVMEGRHCFEHLTVEENLLTGAYTRREGKAAIQRDLEMVYDYFPRLKERRKSQAGYTSGGEQQM
TAIGRALMSKPETILLDEPSMGLAPQLVEQIFEIVKSVNENEGVTFLLAEQNTNVALRFAHYGYILESGRVVMDGPAADL
RENPDVKEFYLGMSEQGRKSFRDVRSYRRRKRWLS

Sequences:

>Translated_275_residues
MFDAAEGQTENVLEVNNIEVIYNHVILVLKGVSLNVPQGGITALLGGNGAGKTTTLKAISGLLASERGEVTKGSIKYRGT
VIQHQDPADIVKSGVVQVMEGRHCFEHLTVEENLLTGAYTRREGKAAIQRDLEMVYDYFPRLKERRKSQAGYTSGGEQQM
TAIGRALMSKPETILLDEPSMGLAPQLVEQIFEIVKSVNENEGVTFLLAEQNTNVALRFAHYGYILESGRVVMDGPAADL
RENPDVKEFYLGMSEQGRKSFRDVRSYRRRKRWLS
>Mature_275_residues
MFDAAEGQTENVLEVNNIEVIYNHVILVLKGVSLNVPQGGITALLGGNGAGKTTTLKAISGLLASERGEVTKGSIKYRGT
VIQHQDPADIVKSGVVQVMEGRHCFEHLTVEENLLTGAYTRREGKAAIQRDLEMVYDYFPRLKERRKSQAGYTSGGEQQM
TAIGRALMSKPETILLDEPSMGLAPQLVEQIFEIVKSVNENEGVTFLLAEQNTNVALRFAHYGYILESGRVVMDGPAADL
RENPDVKEFYLGMSEQGRKSFRDVRSYRRRKRWLS

Specific function: Component of the leucine-specific transport system [H]

COG id: COG0410

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI27477115, Length=179, Percent_Identity=31.8435754189944, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI27436953, Length=218, Percent_Identity=27.9816513761468, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI105990541, Length=198, Percent_Identity=29.2929292929293, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI31657092, Length=221, Percent_Identity=28.5067873303167, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI47078218, Length=191, Percent_Identity=28.2722513089005, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI45446740, Length=191, Percent_Identity=28.2722513089005, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI153792144, Length=222, Percent_Identity=26.5765765765766, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI42490749, Length=221, Percent_Identity=29.8642533936652, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI30795238, Length=175, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI27881501, Length=175, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI150417984, Length=221, Percent_Identity=29.8642533936652, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI21536376, Length=226, Percent_Identity=24.3362831858407, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI116734710, Length=169, Percent_Identity=29.585798816568, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI46592956, Length=217, Percent_Identity=25.3456221198157, Blast_Score=65, Evalue=7e-11,
Organism=Homo sapiens, GI46592964, Length=217, Percent_Identity=25.3456221198157, Blast_Score=65, Evalue=8e-11,
Organism=Homo sapiens, GI46592978, Length=217, Percent_Identity=25.3456221198157, Blast_Score=65, Evalue=8e-11,
Organism=Homo sapiens, GI46592971, Length=217, Percent_Identity=25.3456221198157, Blast_Score=65, Evalue=8e-11,
Organism=Homo sapiens, GI46592915, Length=217, Percent_Identity=25.3456221198157, Blast_Score=65, Evalue=8e-11,
Organism=Homo sapiens, GI46592898, Length=217, Percent_Identity=25.3456221198157, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI87082268, Length=227, Percent_Identity=45.374449339207, Blast_Score=180, Evalue=8e-47,
Organism=Escherichia coli, GI1789864, Length=245, Percent_Identity=28.1632653061224, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788225, Length=239, Percent_Identity=30.5439330543933, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1787029, Length=224, Percent_Identity=29.4642857142857, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790544, Length=241, Percent_Identity=29.8755186721992, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1787089, Length=243, Percent_Identity=27.9835390946502, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1789593, Length=227, Percent_Identity=28.6343612334802, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788761, Length=239, Percent_Identity=30.9623430962343, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1789672, Length=219, Percent_Identity=27.8538812785388, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788450, Length=248, Percent_Identity=28.2258064516129, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787712, Length=236, Percent_Identity=28.8135593220339, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1786872, Length=237, Percent_Identity=27.0042194092827, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87081709, Length=221, Percent_Identity=27.6018099547511, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787370, Length=221, Percent_Identity=30.7692307692308, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1789586, Length=247, Percent_Identity=28.3400809716599, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786253, Length=250, Percent_Identity=26.8, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081791, Length=239, Percent_Identity=27.6150627615063, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786398, Length=244, Percent_Identity=25.8196721311475, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788506, Length=238, Percent_Identity=28.9915966386555, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1790467, Length=240, Percent_Identity=26.6666666666667, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787792, Length=236, Percent_Identity=25.8474576271186, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI87082267, Length=220, Percent_Identity=27.2727272727273, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI87081834, Length=197, Percent_Identity=27.9187817258883, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI48995001, Length=208, Percent_Identity=27.8846153846154, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790525, Length=236, Percent_Identity=27.9661016949153, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788644, Length=237, Percent_Identity=27.0042194092827, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1790190, Length=202, Percent_Identity=26.2376237623762, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787105, Length=238, Percent_Identity=27.3109243697479, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788897, Length=221, Percent_Identity=27.1493212669683, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI48994943, Length=204, Percent_Identity=26.9607843137255, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786703, Length=216, Percent_Identity=27.7777777777778, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787758, Length=217, Percent_Identity=25.3456221198157, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788210, Length=209, Percent_Identity=26.7942583732057, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1786319, Length=261, Percent_Identity=27.5862068965517, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789873, Length=205, Percent_Identity=28.780487804878, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1790162, Length=242, Percent_Identity=23.9669421487603, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788472, Length=223, Percent_Identity=24.6636771300448, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1786803, Length=231, Percent_Identity=22.9437229437229, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI48994997, Length=252, Percent_Identity=25.3968253968254, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787164, Length=211, Percent_Identity=24.1706161137441, Blast_Score=61, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI115533608, Length=215, Percent_Identity=26.5116279069767, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17510237, Length=234, Percent_Identity=24.3589743589744, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24581191, Length=213, Percent_Identity=24.8826291079812, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24581189, Length=213, Percent_Identity=24.8826291079812, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24581193, Length=213, Percent_Identity=24.8826291079812, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24581195, Length=213, Percent_Identity=24.8826291079812, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 30580; Mature: 30580

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDAAEGQTENVLEVNNIEVIYNHVILVLKGVSLNVPQGGITALLGGNGAGKTTTLKAIS
CCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCEEECCCCCEEEEECCCCCCCHHHHHHHH
GLLASERGEVTKGSIKYRGTVIQHQDPADIVKSGVVQVMEGRHCFEHLTVEENLLTGAYT
HHHHHCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
RREGKAAIQRDLEMVYDYFPRLKERRKSQAGYTSGGEQQMTAIGRALMSKPETILLDEPS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEECCC
MGLAPQLVEQIFEIVKSVNENEGVTFLLAEQNTNVALRFAHYGYILESGRVVMDGPAADL
CCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEEHEEEEECCCEEEECCCHHH
RENPDVKEFYLGMSEQGRKSFRDVRSYRRRKRWLS
CCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFDAAEGQTENVLEVNNIEVIYNHVILVLKGVSLNVPQGGITALLGGNGAGKTTTLKAIS
CCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCEEECCCCCEEEEECCCCCCCHHHHHHHH
GLLASERGEVTKGSIKYRGTVIQHQDPADIVKSGVVQVMEGRHCFEHLTVEENLLTGAYT
HHHHHCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
RREGKAAIQRDLEMVYDYFPRLKERRKSQAGYTSGGEQQMTAIGRALMSKPETILLDEPS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEECCC
MGLAPQLVEQIFEIVKSVNENEGVTFLLAEQNTNVALRFAHYGYILESGRVVMDGPAADL
CCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEEHEEEEECCCEEEECCCHHH
RENPDVKEFYLGMSEQGRKSFRDVRSYRRRKRWLS
CCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]