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The map label for this gene is caiD [H]

Identifier: 110677992

GI number: 110677992

Start: 596200

End: 596979

Strand: Direct

Name: caiD [H]

Synonym: RD1_0613

Alternate gene names: 110677992

Gene position: 596200-596979 (Clockwise)

Preceding gene: 110677991

Following gene: 110677993

Centisome position: 14.43

GC content: 59.87

Gene sequence:

>780_bases
ATGGACCCGATTAAAACCCGTCAGCAAGGCATGATTCTTGAAGTCACGCTGGACCGACCCAAGGCCAATGCGATTGATCT
GGCAACCTCGCGCGTGATGGGAGAGGTCTTTGCCAGCTTCAGAGATGACCCTGATCTGCGCGTGGCGATCCTGACCGGGG
CGGGTGAAAAGTTCTTTTGCCCGGGCTGGGATCTCAAGGCTGCGGCAGAGGGCGATGCGGTTGATGGCGATTATGGTGTC
GGTGGTTTTGGCGGCCTGCAGGAATTGCGCGCGATGAACAAGCCGGTGATTGCAGCCGTCAACGGCATTGCCTGCGGGGG
CGGACTGGAACTGGCCCTGAGCGCGGATATGATTCTGGCCGCTGATCACGCCAGCTTTGCCCTGCCTGAAATCCGCTCTG
GCACGATTGCGGATGCGGCTTCGATCAAGCTTCCCAAACGTATCCCCTACCACATCGCCATGGAACTTTTGCTGACCGGG
CGCTGGTTCGACCCGCAAGAAGCCAAAGGCTGGGGGCTGGTCAATGAAATCCTGCCCGCCGCTGATCTGTTGCCCCGCGC
TTGGGAGTTGGCGGAGTTGCTGGCCTCCGGCCCGCCACTGGTCTACGCCGCAATCAAAGAGGTGGTCCGAGAGGCAGAGG
ACGCCAAATTTCAGGACATCATGAACCGGATCACCAAGCGGCAGCTTGAAACCATCGACGTGCTCTATAGTTCCGAGGAC
CAGCTTGAGGGCGCGACCGCTTTTGCGGAGAAACGCGACCCGGTCTGGAAAGGACGGTAG

Upstream 100 bases:

>100_bases
GAGGTTGAAATCAACCCGCTGATGTGCACCCCCGACGCAGCTATCGCGGCGGATGCCCTTATCACACGGCTAATTCCCCA
ATTCCCGGCAGGAGAGCGAC

Downstream 100 bases:

>100_bases
GGGGTTGTCTCTGGCGGGGCGAGGCGTCACGTTGCGCCTATGACGCAGAACACTCTTTTGTCACTCGGGCATGGCTTTAG
CGCGCGGGCCCTTGCCACCC

Product: carnitinyl-CoA dehydratase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MDPIKTRQQGMILEVTLDRPKANAIDLATSRVMGEVFASFRDDPDLRVAILTGAGEKFFCPGWDLKAAAEGDAVDGDYGV
GGFGGLQELRAMNKPVIAAVNGIACGGGLELALSADMILAADHASFALPEIRSGTIADAASIKLPKRIPYHIAMELLLTG
RWFDPQEAKGWGLVNEILPAADLLPRAWELAELLASGPPLVYAAIKEVVREAEDAKFQDIMNRITKRQLETIDVLYSSED
QLEGATAFAEKRDPVWKGR

Sequences:

>Translated_259_residues
MDPIKTRQQGMILEVTLDRPKANAIDLATSRVMGEVFASFRDDPDLRVAILTGAGEKFFCPGWDLKAAAEGDAVDGDYGV
GGFGGLQELRAMNKPVIAAVNGIACGGGLELALSADMILAADHASFALPEIRSGTIADAASIKLPKRIPYHIAMELLLTG
RWFDPQEAKGWGLVNEILPAADLLPRAWELAELLASGPPLVYAAIKEVVREAEDAKFQDIMNRITKRQLETIDVLYSSED
QLEGATAFAEKRDPVWKGR
>Mature_259_residues
MDPIKTRQQGMILEVTLDRPKANAIDLATSRVMGEVFASFRDDPDLRVAILTGAGEKFFCPGWDLKAAAEGDAVDGDYGV
GGFGGLQELRAMNKPVIAAVNGIACGGGLELALSADMILAADHASFALPEIRSGTIADAASIKLPKRIPYHIAMELLLTG
RWFDPQEAKGWGLVNEILPAADLLPRAWELAELLASGPPLVYAAIKEVVREAEDAKFQDIMNRITKRQLETIDVLYSSED
QLEGATAFAEKRDPVWKGR

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=245, Percent_Identity=36.734693877551, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI31542718, Length=260, Percent_Identity=30.7692307692308, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI70995211, Length=258, Percent_Identity=31.3953488372093, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI221142681, Length=259, Percent_Identity=54.0540540540541, Blast_Score=269, Evalue=1e-73,
Organism=Escherichia coli, GI1787659, Length=260, Percent_Identity=36.1538461538462, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1788597, Length=250, Percent_Identity=30.4, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI87082183, Length=263, Percent_Identity=26.9961977186312, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788682, Length=189, Percent_Identity=29.6296296296296, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787660, Length=268, Percent_Identity=30.5970149253731, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25145438, Length=247, Percent_Identity=35.6275303643725, Blast_Score=133, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI17554946, Length=251, Percent_Identity=33.8645418326693, Blast_Score=120, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17560910, Length=253, Percent_Identity=31.6205533596838, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17534483, Length=255, Percent_Identity=27.843137254902, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17549921, Length=247, Percent_Identity=29.5546558704453, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17540714, Length=237, Percent_Identity=28.2700421940928, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17535521, Length=239, Percent_Identity=26.7782426778243, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17540306, Length=189, Percent_Identity=27.5132275132275, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17536985, Length=266, Percent_Identity=25.5639097744361, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25144160, Length=212, Percent_Identity=26.4150943396226, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI25144157, Length=212, Percent_Identity=26.4150943396226, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI20129971, Length=243, Percent_Identity=35.8024691358025, Blast_Score=130, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24653477, Length=243, Percent_Identity=35.8024691358025, Blast_Score=130, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24653139, Length=263, Percent_Identity=33.4600760456274, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21357171, Length=192, Percent_Identity=35.9375, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19920382, Length=235, Percent_Identity=31.4893617021277, Blast_Score=87, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24650670, Length=227, Percent_Identity=29.0748898678414, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19922422, Length=259, Percent_Identity=22.3938223938224, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 28032; Mature: 28032

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPIKTRQQGMILEVTLDRPKANAIDLATSRVMGEVFASFRDDPDLRVAILTGAGEKFFC
CCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEC
PGWDLKAAAEGDAVDGDYGVGGFGGLQELRAMNKPVIAAVNGIACGGGLELALSADMILA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCEEECCCEEEEEECCEEEE
ADHASFALPEIRSGTIADAASIKLPKRIPYHIAMELLLTGRWFDPQEAKGWGLVNEILPA
ECCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCH
ADLLPRAWELAELLASGPPLVYAAIKEVVREAEDAKFQDIMNRITKRQLETIDVLYSSED
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCH
QLEGATAFAEKRDPVWKGR
HHCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDPIKTRQQGMILEVTLDRPKANAIDLATSRVMGEVFASFRDDPDLRVAILTGAGEKFFC
CCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEC
PGWDLKAAAEGDAVDGDYGVGGFGGLQELRAMNKPVIAAVNGIACGGGLELALSADMILA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCEEECCCEEEEEECCEEEE
ADHASFALPEIRSGTIADAASIKLPKRIPYHIAMELLLTGRWFDPQEAKGWGLVNEILPA
ECCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCH
ADLLPRAWELAELLASGPPLVYAAIKEVVREAEDAKFQDIMNRITKRQLETIDVLYSSED
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCH
QLEGATAFAEKRDPVWKGR
HHCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA