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The map label for this gene is pncA [H]

Identifier: 110677966

GI number: 110677966

Start: 567843

End: 568451

Strand: Direct

Name: pncA [H]

Synonym: RD1_0585

Alternate gene names: 110677966

Gene position: 567843-568451 (Clockwise)

Preceding gene: 110677959

Following gene: 110677967

Centisome position: 13.74

GC content: 57.14

Gene sequence:

>609_bases
ATGAGCCACGCTTTGATTGTCATTGACGTTCAACTGGATTTCTGTCCGGGTGGCGCACTTGCCGTGCCGGATGGGGATGG
GATCATCGCGGGCATCAACGCACTCATGCCGCAGGCGGATGCTGTTGTGCTGACACAGGATTGGCACCCGGCGGGTCACT
CCTCCTTTGCCACTTCGCACAAGGGGCATGCGCCTTATGACGTGATCGACATGGATTATGGCCCGCAGGTGTTGTGGCCG
CACCATTGTGTGCAGGGGGCTGACGGCGCGCGGTTCCATCCCGATCTACAAAGTGACGGCGCAGATCTGATCCTGCGCAA
AGGGTATAATCCGGCCATAGACAGCTATTCCGCGTTCTTTGAAAATGATCACAAAACGCCAACAGGGCTTGAAGGGTACC
TTCGCAGCCGGGGTATCGAAAAACTGACGCTGGTCGGGCTGGCATTCGATTTCTGCGTCAACTATTCAGCACTCGATGCC
GCCAAACTGGAATTCGACGTCACAGTACGCGAAGATCTGTGCCGCGCGATTGACCTTGATGGTTCCCGTGATGCAGCGCG
TCAGGCCATGCAGAATGCGGGCGTGCGTCTGGAGACAGTGCATGGTTGA

Upstream 100 bases:

>100_bases
TGCGCGCGCTTTCAGGCAAGAGTGTTGTAGCGCATCACCGCCATTGGCATTGACGGGCTGCACGCAGGCGCTTAGCCATA
TCCCAGACGGGAGAAAACGC

Downstream 100 bases:

>100_bases
TATCGCAACCCGCGTCTGGAACCACAAATGGAAGATCGACCCGATTGTGCGCTCGTTGATCGATACGGATTTTTACAAGC
TTCTGATGTGTCAGTCGGTT

Product: pyrazinamidase/nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MSHALIVIDVQLDFCPGGALAVPDGDGIIAGINALMPQADAVVLTQDWHPAGHSSFATSHKGHAPYDVIDMDYGPQVLWP
HHCVQGADGARFHPDLQSDGADLILRKGYNPAIDSYSAFFENDHKTPTGLEGYLRSRGIEKLTLVGLAFDFCVNYSALDA
AKLEFDVTVREDLCRAIDLDGSRDAARQAMQNAGVRLETVHG

Sequences:

>Translated_202_residues
MSHALIVIDVQLDFCPGGALAVPDGDGIIAGINALMPQADAVVLTQDWHPAGHSSFATSHKGHAPYDVIDMDYGPQVLWP
HHCVQGADGARFHPDLQSDGADLILRKGYNPAIDSYSAFFENDHKTPTGLEGYLRSRGIEKLTLVGLAFDFCVNYSALDA
AKLEFDVTVREDLCRAIDLDGSRDAARQAMQNAGVRLETVHG
>Mature_201_residues
SHALIVIDVQLDFCPGGALAVPDGDGIIAGINALMPQADAVVLTQDWHPAGHSSFATSHKGHAPYDVIDMDYGPQVLWPH
HCVQGADGARFHPDLQSDGADLILRKGYNPAIDSYSAFFENDHKTPTGLEGYLRSRGIEKLTLVGLAFDFCVNYSALDAA
KLEFDVTVREDLCRAIDLDGSRDAARQAMQNAGVRLETVHG

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=202, Percent_Identity=43.5643564356436, Blast_Score=170, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI193206735, Length=187, Percent_Identity=36.8983957219251, Blast_Score=109, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17543130, Length=187, Percent_Identity=36.8983957219251, Blast_Score=108, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71999339, Length=221, Percent_Identity=31.2217194570136, Blast_Score=90, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6321401, Length=196, Percent_Identity=35.2040816326531, Blast_Score=105, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24648247, Length=221, Percent_Identity=33.0316742081448, Blast_Score=107, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 21814; Mature: 21682

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHALIVIDVQLDFCPGGALAVPDGDGIIAGINALMPQADAVVLTQDWHPAGHSSFATSH
CCCEEEEEEEEEEECCCCEEECCCCCCEEEEHHHHCCCCCEEEEEECCCCCCCCCCCCCC
KGHAPYDVIDMDYGPQVLWPHHCVQGADGARFHPDLQSDGADLILRKGYNPAIDSYSAFF
CCCCCCEEEECCCCCCEECCHHHHCCCCCCEECCCCCCCCCEEEEECCCCCCHHHHHHHH
ENDHKTPTGLEGYLRSRGIEKLTLVGLAFDFCVNYSALDAAKLEFDVTVREDLCRAIDLD
CCCCCCCCCHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCCEEEEEEEEHHHHHHHHHCCC
GSRDAARQAMQNAGVRLETVHG
CCHHHHHHHHHHCCCEEEEECC
>Mature Secondary Structure 
SHALIVIDVQLDFCPGGALAVPDGDGIIAGINALMPQADAVVLTQDWHPAGHSSFATSH
CCEEEEEEEEEEECCCCEEECCCCCCEEEEHHHHCCCCCEEEEEECCCCCCCCCCCCCC
KGHAPYDVIDMDYGPQVLWPHHCVQGADGARFHPDLQSDGADLILRKGYNPAIDSYSAFF
CCCCCCEEEECCCCCCEECCHHHHCCCCCCEECCCCCCCCCEEEEECCCCCCHHHHHHHH
ENDHKTPTGLEGYLRSRGIEKLTLVGLAFDFCVNYSALDAAKLEFDVTVREDLCRAIDLD
CCCCCCCCCHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCCEEEEEEEEHHHHHHHHHCCC
GSRDAARQAMQNAGVRLETVHG
CCHHHHHHHHHHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]