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The map label for this gene is purR [H]

Identifier: 110677876

GI number: 110677876

Start: 476714

End: 477712

Strand: Direct

Name: purR [H]

Synonym: RD1_0490

Alternate gene names: 110677876

Gene position: 476714-477712 (Clockwise)

Preceding gene: 110677867

Following gene: 110677877

Centisome position: 11.53

GC content: 58.16

Gene sequence:

>999_bases
ATGACCGTTACTTTAAAGGATGTGGCCGAACGTGCGGGTGTATCGCGCTCTGCGGTGTCGCGCACATTTACGGCGGGCGC
CTCTGTTTCGCCGAAAACGCGGGCCAAGGTTGAGAAGGCCGCCGATGATCTGGGTTATCGCCCGAACGCGTTGGCCTCTT
CCCTGACCACGGGGCGCACGAAGCTGATCGGTCTGATCTCGAACAACTTTCACAACCCGGTGTTTCTGGAGGTATTTGAC
CTCTTCACCCGGTGCCTTCAGGATCGCGGCCTGCGCCCGCTGCTGGTCAATCTCACCGATGAAACGGACCCGGAAAATTC
GCTGCGGCTTTTGCAGCAATACTCGGTTGATGGGGTGATTGTGGCCTCCTCCACGCTGTCGCAGGAATTCCCGCTGGCCT
TTCAGACGTCGCGGATACCTGTTGTGCATTCCTTTGGAAAATTCGCCCATGCGCCGCGCGTGCATGTTGTTGGTATCGAC
AATATCGAGTGTGGGCGCATGGCGGCGCGTACCCTGATCGCGCGCGGTTACAATCGTCTCGGATTCCTCGGTGGCCCAGC
CGATGCCACCTCGACCGTGGATCGCCGCACGGGCTTTCTGGAAGTGATCGAGGCTGTGGATGATGTCACCGTGTCAGACA
GTTTTGCCACCGCATATTCCTTTGACGCGGGGCGCAACGAGATGCTCTCCTTGCTGCGAAACACCCCTGCGGATGCGTAT
TTCTGCGGTGACGATGTTTTGTCCATCGGCGCATTGAGCGCCATCCGCGAAGCGGGTCTGCGGGTCCCGGAGGATATCGG
AATTATCGGTCTGAACGACATGGAGATGTCGCGCTGGCAGAACATCAATCTCACAACGATTCGCCAGCCTATACCGCAGA
TTATCGACAGTTCGATTGATTTGGTCATGGCGCTGCTGGAGAAGCCGGACCACCAGCCTGAGGCGCGGCTTTTTGCATGC
GACATCATCGAAAGAGGTACGCTGCGCCCTTTGCCTTAG

Upstream 100 bases:

>100_bases
CGGCAGGAAGAAAGCACAGGTTCGGTATTATTGCAACCGGTTGCAAGAAATCAAATCCTGTGTTCAGATCACGTTGTGCT
GCTGAGTGTGAAAGGCTGGA

Downstream 100 bases:

>100_bases
AAAACCGTGCATCGGGCGCGGATGCGACATGCGTCGGGGACTTGACGTCGGTGCTGGACAGCTTGGGCGCTCGTTTCAAA
GATGACGGTCTTGGCCTTGC

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MTVTLKDVAERAGVSRSAVSRTFTAGASVSPKTRAKVEKAADDLGYRPNALASSLTTGRTKLIGLISNNFHNPVFLEVFD
LFTRCLQDRGLRPLLVNLTDETDPENSLRLLQQYSVDGVIVASSTLSQEFPLAFQTSRIPVVHSFGKFAHAPRVHVVGID
NIECGRMAARTLIARGYNRLGFLGGPADATSTVDRRTGFLEVIEAVDDVTVSDSFATAYSFDAGRNEMLSLLRNTPADAY
FCGDDVLSIGALSAIREAGLRVPEDIGIIGLNDMEMSRWQNINLTTIRQPIPQIIDSSIDLVMALLEKPDHQPEARLFAC
DIIERGTLRPLP

Sequences:

>Translated_332_residues
MTVTLKDVAERAGVSRSAVSRTFTAGASVSPKTRAKVEKAADDLGYRPNALASSLTTGRTKLIGLISNNFHNPVFLEVFD
LFTRCLQDRGLRPLLVNLTDETDPENSLRLLQQYSVDGVIVASSTLSQEFPLAFQTSRIPVVHSFGKFAHAPRVHVVGID
NIECGRMAARTLIARGYNRLGFLGGPADATSTVDRRTGFLEVIEAVDDVTVSDSFATAYSFDAGRNEMLSLLRNTPADAY
FCGDDVLSIGALSAIREAGLRVPEDIGIIGLNDMEMSRWQNINLTTIRQPIPQIIDSSIDLVMALLEKPDHQPEARLFAC
DIIERGTLRPLP
>Mature_331_residues
TVTLKDVAERAGVSRSAVSRTFTAGASVSPKTRAKVEKAADDLGYRPNALASSLTTGRTKLIGLISNNFHNPVFLEVFDL
FTRCLQDRGLRPLLVNLTDETDPENSLRLLQQYSVDGVIVASSTLSQEFPLAFQTSRIPVVHSFGKFAHAPRVHVVGIDN
IECGRMAARTLIARGYNRLGFLGGPADATSTVDRRTGFLEVIEAVDDVTVSDSFATAYSFDAGRNEMLSLLRNTPADAYF
CGDDVLSIGALSAIREAGLRVPEDIGIIGLNDMEMSRWQNINLTTIRQPIPQIIDSSIDLVMALLEKPDHQPEARLFACD
IIERGTLRPLP

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=329, Percent_Identity=31.306990881459, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1790369, Length=332, Percent_Identity=29.2168674698795, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1790194, Length=333, Percent_Identity=30.9309309309309, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1788474, Length=305, Percent_Identity=29.5081967213115, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1789068, Length=336, Percent_Identity=26.7857142857143, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI48994940, Length=289, Percent_Identity=27.3356401384083, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787580, Length=308, Percent_Identity=27.9220779220779, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1789202, Length=292, Percent_Identity=26.7123287671233, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1786540, Length=308, Percent_Identity=27.2727272727273, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790715, Length=330, Percent_Identity=23.9393939393939, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787906, Length=266, Percent_Identity=27.4436090225564, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1786268, Length=316, Percent_Identity=25.3164556962025, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1789456, Length=300, Percent_Identity=27.3333333333333, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36233; Mature: 36102

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTLKDVAERAGVSRSAVSRTFTAGASVSPKTRAKVEKAADDLGYRPNALASSLTTGRT
CEEEHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCHH
KLIGLISNNFHNPVFLEVFDLFTRCLQDRGLRPLLVNLTDETDPENSLRLLQQYSVDGVI
HEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEE
VASSTLSQEFPLAFQTSRIPVVHSFGKFAHAPRVHVVGIDNIECGRMAARTLIARGYNRL
EECCCCCCCCCCEEECCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCC
GFLGGPADATSTVDRRTGFLEVIEAVDDVTVSDSFATAYSFDAGRNEMLSLLRNTPADAY
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEECCCCHHHHHHHHHCCCCCEE
FCGDDVLSIGALSAIREAGLRVPEDIGIIGLNDMEMSRWQNINLTTIRQPIPQIIDSSID
EECCHHHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHCCCCCCHHHHHHHHHHHCCHHH
LVMALLEKPDHQPEARLFACDIIERGTLRPLP
HHHHHHHCCCCCCCHHEEEEHHHCCCCCCCCC
>Mature Secondary Structure 
TVTLKDVAERAGVSRSAVSRTFTAGASVSPKTRAKVEKAADDLGYRPNALASSLTTGRT
EEEHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCHH
KLIGLISNNFHNPVFLEVFDLFTRCLQDRGLRPLLVNLTDETDPENSLRLLQQYSVDGVI
HEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEE
VASSTLSQEFPLAFQTSRIPVVHSFGKFAHAPRVHVVGIDNIECGRMAARTLIARGYNRL
EECCCCCCCCCCEEECCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCC
GFLGGPADATSTVDRRTGFLEVIEAVDDVTVSDSFATAYSFDAGRNEMLSLLRNTPADAY
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEECCCCHHHHHHHHHCCCCCEE
FCGDDVLSIGALSAIREAGLRVPEDIGIIGLNDMEMSRWQNINLTTIRQPIPQIIDSSID
EECCHHHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHCCCCCCHHHHHHHHHHHCCHHH
LVMALLEKPDHQPEARLFACDIIERGTLRPLP
HHHHHHHCCCCCCCHHEEEEHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA