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The map label for this gene is gacS [H]

Identifier: 110677757

GI number: 110677757

Start: 350567

End: 352768

Strand: Direct

Name: gacS [H]

Synonym: RD1_0357

Alternate gene names: 110677757

Gene position: 350567-352768 (Clockwise)

Preceding gene: 110677756

Following gene: 110677761

Centisome position: 8.48

GC content: 54.27

Gene sequence:

>2202_bases
TTGCTTGAACTCAAGCAGGCCGAACTCTTTGCCGCGAATCGAAAACTGGGGCTCCATGCGCGCCAACTGTCGAACGAGAT
CGTCGAAACGCGCGCCGAAGTCGCCAATGTGCGGGATGAAAACAAACGCGTCAAATCGGACCTTTCCGCCGCCCACAAAC
AGATCGAGCGTGTGGAACGCAGGCTGTGGCAGTCCATTGAAACGATAAATGACGGGTTCGCGTTTTATTCGCCTGATCTG
GAACTTATCATGGCAAATAATTCCTATCTTGCTGTCTTTGACGGGCTCGAAGACATGCAACCCGGCGTCAGCTATGTGAC
CGTGCTGCAGTTTCTCACAGAGGAAGGCATCGTGAATACGGGTGACTTGCGCCCGGCTGAATGGCGTCAGAAAATGATTG
AACGTGTCCAGTCGCCCGAACCCGAAGCTGTCGTTATTCAACTTTGGAACAATGAATACATTCGGATGATTGATCGGCGC
GGACCTGACGGGGATATAATCTCCCTTGGCATCAACATCACCGCTGCCGTGCGATACGAGGAGAAACTCGAGGCCGCGCG
CGCCAGTGCGGAGGCGGCAAGTCGAGCGAAATCCGCCTTCCTCGCCAATATGAGCCATGAAATCAGAACCCCCATGAATG
GTGTCGTCGGCATGGCGGATGTTCTGACGGAAACACCGCTGAGCGAAGAGCAAAGGCTCTATGTCGACACCATCAAGAAC
TCTGGTGAGGCGCTGCTCGTCATCATCAATGACGTGCTGGACTATTCCAAGATCGAAGCGGAAAAACTCCAGTTGCACCC
CGAACCTTTCAACCTTGAAAAATCCATTCACGAAGTGTTGCGCCTGTTGCAGTCAATCGCGCGCGACAAGGGTATTTTGC
TGAGCGTTGATTACGCGCTGTTTTTGTCCTCTGACTTCATTGGCGATCCGGGCCGTTTGCGTCAGATCCTGACCAATCTG
CTTGGCAATGCCGTAAAATTTACGACTGAAGGGTCCGTGACAGTGCGCGTCTCGGGCAAACCTGACGAAGCGACGGGCAA
AGTGATCTTGCAAATCAGGGTCGAAGATACCGGCATCGGCATTCCAGAGGATATGATTGATCACGTCTTTGGCGAGTTCA
ATCAGGTTGAAAGCGAACGGAACCGCCAGTTTGACGGTACCGGTCTGGGTCTGGCGATCACCAAAAAGCTGATTAAGATG
ATGAATGGGCAGATTTGGCTCACTTCCGAAGAAGGCGTTGGCTCTTGCTTCGGGTTTGAGATTGCGCTCGAACCTACGGG
CCCTGTCACACTGGCCCACCCGGCGCTCGACCCTGGATTGCGCCACGTCATGGTTGTTGATGATTTTCCGCTCAACCGCG
ACATACTTCAGCGACAACTGGAAGAGATTGGCCTAAAGGTGACGGCCTGCCAGGATGCAGCCACTGCGCTTGCCATGCTC
GATAGCTCGGTTGATCTTGTGATGGTTGATCACATCATGGCCGACGTGAACGGCCTGCAGTTTGCGCAAACAGCGCGCAA
GGCTGGTCATACGCAGCAGATCATTTTGCTCAGCAGTAACCACGGTTATGCCAGTAAGGACCCGCTGAGCGACAGTGTCG
ATATCATTGCGCAACGACCCTATCTGCGCAGTGACCTGTTTGGCTGGCTCGAAACGGTCAAGCCGGTGAGCAATAACAAC
GCAGCACCGGATAAGGCTGATGGTGCCGCATCACCAGCCGTCTTTTCACACCGCAAAACCGTCTCGCCGTCTGATGCACA
ACCCGTTGAGCCGACAGCACCCCGCAAGATGCGCATACTCGCCGCCGAGGATAACAAGACCAATCGGCTGGTCTTTGGCA
AGATGATCAAAGCGCTTGATATCGAACTGCAATTTGCAAATGACGGTCAGGAAGCCGTCGATGCCTACCCGGAATTCCAG
CCTGATCTGATTTTCATGGATATCTCAATGCCGCGTCTGGATGGCAAACAGGCGACCCGCGCGATCCGCGAAATTGAAAA
AAGCACTAGTGCCCATGTGCCGATCATCGCTTTGACCGCCCACGCGATGAGCGGGGATGACAAGGATATTCTTGAGGCTG
GCCTCGACCATTACATGACCAAGCCACTGCGCAAGAACGAAATCATCGGCAAAATCATGGACTTCCGGCCCAGCGATGCC
GCGCACCCCTGCCCCGATGACATGGATCAGGAAGCGGGATAG

Upstream 100 bases:

>100_bases
GATTGCGGCGCAAACCGGTGTTTTAATCCATGCGTATATCTGAATGAGCATTGCCAATAAACTGGCCCAGGAAAGGCGCA
GTCGACTTGCTGCGGAGCGT

Downstream 100 bases:

>100_bases
GGCCGAATGAAAACGGGCTTGTCCGGCGTCGACAACTCCGGTTGATACGCATAGCCACCGGTGTCGAAACCGGTAAGTGC
GTCCGGATCAGAAAGCCTGT

Product: hybrid sensory histidine kinase, putative

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 733; Mature: 733

Protein sequence:

>733_residues
MLELKQAELFAANRKLGLHARQLSNEIVETRAEVANVRDENKRVKSDLSAAHKQIERVERRLWQSIETINDGFAFYSPDL
ELIMANNSYLAVFDGLEDMQPGVSYVTVLQFLTEEGIVNTGDLRPAEWRQKMIERVQSPEPEAVVIQLWNNEYIRMIDRR
GPDGDIISLGINITAAVRYEEKLEAARASAEAASRAKSAFLANMSHEIRTPMNGVVGMADVLTETPLSEEQRLYVDTIKN
SGEALLVIINDVLDYSKIEAEKLQLHPEPFNLEKSIHEVLRLLQSIARDKGILLSVDYALFLSSDFIGDPGRLRQILTNL
LGNAVKFTTEGSVTVRVSGKPDEATGKVILQIRVEDTGIGIPEDMIDHVFGEFNQVESERNRQFDGTGLGLAITKKLIKM
MNGQIWLTSEEGVGSCFGFEIALEPTGPVTLAHPALDPGLRHVMVVDDFPLNRDILQRQLEEIGLKVTACQDAATALAML
DSSVDLVMVDHIMADVNGLQFAQTARKAGHTQQIILLSSNHGYASKDPLSDSVDIIAQRPYLRSDLFGWLETVKPVSNNN
AAPDKADGAASPAVFSHRKTVSPSDAQPVEPTAPRKMRILAAEDNKTNRLVFGKMIKALDIELQFANDGQEAVDAYPEFQ
PDLIFMDISMPRLDGKQATRAIREIEKSTSAHVPIIALTAHAMSGDDKDILEAGLDHYMTKPLRKNEIIGKIMDFRPSDA
AHPCPDDMDQEAG

Sequences:

>Translated_733_residues
MLELKQAELFAANRKLGLHARQLSNEIVETRAEVANVRDENKRVKSDLSAAHKQIERVERRLWQSIETINDGFAFYSPDL
ELIMANNSYLAVFDGLEDMQPGVSYVTVLQFLTEEGIVNTGDLRPAEWRQKMIERVQSPEPEAVVIQLWNNEYIRMIDRR
GPDGDIISLGINITAAVRYEEKLEAARASAEAASRAKSAFLANMSHEIRTPMNGVVGMADVLTETPLSEEQRLYVDTIKN
SGEALLVIINDVLDYSKIEAEKLQLHPEPFNLEKSIHEVLRLLQSIARDKGILLSVDYALFLSSDFIGDPGRLRQILTNL
LGNAVKFTTEGSVTVRVSGKPDEATGKVILQIRVEDTGIGIPEDMIDHVFGEFNQVESERNRQFDGTGLGLAITKKLIKM
MNGQIWLTSEEGVGSCFGFEIALEPTGPVTLAHPALDPGLRHVMVVDDFPLNRDILQRQLEEIGLKVTACQDAATALAML
DSSVDLVMVDHIMADVNGLQFAQTARKAGHTQQIILLSSNHGYASKDPLSDSVDIIAQRPYLRSDLFGWLETVKPVSNNN
AAPDKADGAASPAVFSHRKTVSPSDAQPVEPTAPRKMRILAAEDNKTNRLVFGKMIKALDIELQFANDGQEAVDAYPEFQ
PDLIFMDISMPRLDGKQATRAIREIEKSTSAHVPIIALTAHAMSGDDKDILEAGLDHYMTKPLRKNEIIGKIMDFRPSDA
AHPCPDDMDQEAG
>Mature_733_residues
MLELKQAELFAANRKLGLHARQLSNEIVETRAEVANVRDENKRVKSDLSAAHKQIERVERRLWQSIETINDGFAFYSPDL
ELIMANNSYLAVFDGLEDMQPGVSYVTVLQFLTEEGIVNTGDLRPAEWRQKMIERVQSPEPEAVVIQLWNNEYIRMIDRR
GPDGDIISLGINITAAVRYEEKLEAARASAEAASRAKSAFLANMSHEIRTPMNGVVGMADVLTETPLSEEQRLYVDTIKN
SGEALLVIINDVLDYSKIEAEKLQLHPEPFNLEKSIHEVLRLLQSIARDKGILLSVDYALFLSSDFIGDPGRLRQILTNL
LGNAVKFTTEGSVTVRVSGKPDEATGKVILQIRVEDTGIGIPEDMIDHVFGEFNQVESERNRQFDGTGLGLAITKKLIKM
MNGQIWLTSEEGVGSCFGFEIALEPTGPVTLAHPALDPGLRHVMVVDDFPLNRDILQRQLEEIGLKVTACQDAATALAML
DSSVDLVMVDHIMADVNGLQFAQTARKAGHTQQIILLSSNHGYASKDPLSDSVDIIAQRPYLRSDLFGWLETVKPVSNNN
AAPDKADGAASPAVFSHRKTVSPSDAQPVEPTAPRKMRILAAEDNKTNRLVFGKMIKALDIELQFANDGQEAVDAYPEFQ
PDLIFMDISMPRLDGKQATRAIREIEKSTSAHVPIIALTAHAMSGDDKDILEAGLDHYMTKPLRKNEIIGKIMDFRPSDA
AHPCPDDMDQEAG

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=540, Percent_Identity=32.4074074074074, Blast_Score=259, Evalue=6e-70,
Organism=Escherichia coli, GI145693157, Length=546, Percent_Identity=29.6703296703297, Blast_Score=216, Evalue=5e-57,
Organism=Escherichia coli, GI48994928, Length=360, Percent_Identity=36.6666666666667, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI87081816, Length=325, Percent_Identity=38.7692307692308, Blast_Score=195, Evalue=8e-51,
Organism=Escherichia coli, GI1788713, Length=347, Percent_Identity=33.1412103746398, Blast_Score=165, Evalue=1e-41,
Organism=Escherichia coli, GI1786600, Length=229, Percent_Identity=31.8777292576419, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI87082128, Length=229, Percent_Identity=28.3842794759825, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1786783, Length=220, Percent_Identity=29.5454545454545, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1790436, Length=222, Percent_Identity=28.8288288288288, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1786912, Length=240, Percent_Identity=27.5, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788549, Length=256, Percent_Identity=25.390625, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788393, Length=242, Percent_Identity=24.3801652892562, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1790861, Length=239, Percent_Identity=25.5230125523013, Blast_Score=67, Evalue=6e-12,
Organism=Escherichia coli, GI1787894, Length=237, Percent_Identity=25.3164556962025, Blast_Score=63, Evalue=6e-11,
Organism=Escherichia coli, GI1789808, Length=218, Percent_Identity=27.9816513761468, Blast_Score=63, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=125, Percent_Identity=36, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323034, Length=144, Percent_Identity=30.5555555555556, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6322000, Length=150, Percent_Identity=27.3333333333333, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 81132; Mature: 81132

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLELKQAELFAANRKLGLHARQLSNEIVETRAEVANVRDENKRVKSDLSAAHKQIERVER
CCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
RLWQSIETINDGFAFYSPDLELIMANNSYLAVFDGLEDMQPGVSYVTVLQFLTEEGIVNT
HHHHHHHHHHCCCEEECCCEEEEEECCCEEEEECCHHHCCCCHHHHHHHHHHHHCCCCCC
GDLRPAEWRQKMIERVQSPEPEAVVIQLWNNEYIRMIDRRGPDGDIISLGINITAAVRYE
CCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEHHHCCCCCCCEEEEECEEEEEEEHH
EKLEAARASAEAASRAKSAFLANMSHEIRTPMNGVVGMADVLTETPLSEEQRLYVDTIKN
HHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCC
SGEALLVIINDVLDYSKIEAEKLQLHPEPFNLEKSIHEVLRLLQSIARDKGILLSVDYAL
CCCEEEEEEHHHHCHHHCCHHHHEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECHHE
FLSSDFIGDPGRLRQILTNLLGNAVKFTTEGSVTVRVSGKPDEATGKVILQIRVEDTGIG
EECCCCCCCHHHHHHHHHHHHCCCEEEEECCEEEEEECCCCCCCCCEEEEEEEEECCCCC
IPEDMIDHVFGEFNQVESERNRQFDGTGLGLAITKKLIKMMNGQIWLTSEEGVGSCFGFE
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCEEEEE
IALEPTGPVTLAHPALDPGLRHVMVVDDFPLNRDILQRQLEEIGLKVTACQDAATALAML
EEECCCCCEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCEEEEHHHHHHHHHHH
DSSVDLVMVDHIMADVNGLQFAQTARKAGHTQQIILLSSNHGYASKDPLSDSVDIIAQRP
CCCCCEEEHHHHHHHCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHCCCC
YLRSDLFGWLETVKPVSNNNAAPDKADGAASPAVFSHRKTVSPSDAQPVEPTAPRKMRIL
CHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCEEEEE
AAEDNKTNRLVFGKMIKALDIELQFANDGQEAVDAYPEFQPDLIFMDISMPRLDGKQATR
EECCCCCCEEHHHHHHHHHEEEEEECCCCHHHHHCCCCCCCCEEEEEECCCCCCCHHHHH
AIREIEKSTSAHVPIIALTAHAMSGDDKDILEAGLDHYMTKPLRKNEIIGKIMDFRPSDA
HHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCC
AHPCPDDMDQEAG
CCCCCCCCCCCCC
>Mature Secondary Structure
MLELKQAELFAANRKLGLHARQLSNEIVETRAEVANVRDENKRVKSDLSAAHKQIERVER
CCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
RLWQSIETINDGFAFYSPDLELIMANNSYLAVFDGLEDMQPGVSYVTVLQFLTEEGIVNT
HHHHHHHHHHCCCEEECCCEEEEEECCCEEEEECCHHHCCCCHHHHHHHHHHHHCCCCCC
GDLRPAEWRQKMIERVQSPEPEAVVIQLWNNEYIRMIDRRGPDGDIISLGINITAAVRYE
CCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEHHHCCCCCCCEEEEECEEEEEEEHH
EKLEAARASAEAASRAKSAFLANMSHEIRTPMNGVVGMADVLTETPLSEEQRLYVDTIKN
HHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCC
SGEALLVIINDVLDYSKIEAEKLQLHPEPFNLEKSIHEVLRLLQSIARDKGILLSVDYAL
CCCEEEEEEHHHHCHHHCCHHHHEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECHHE
FLSSDFIGDPGRLRQILTNLLGNAVKFTTEGSVTVRVSGKPDEATGKVILQIRVEDTGIG
EECCCCCCCHHHHHHHHHHHHCCCEEEEECCEEEEEECCCCCCCCCEEEEEEEEECCCCC
IPEDMIDHVFGEFNQVESERNRQFDGTGLGLAITKKLIKMMNGQIWLTSEEGVGSCFGFE
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCEEEEE
IALEPTGPVTLAHPALDPGLRHVMVVDDFPLNRDILQRQLEEIGLKVTACQDAATALAML
EEECCCCCEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCEEEEHHHHHHHHHHH
DSSVDLVMVDHIMADVNGLQFAQTARKAGHTQQIILLSSNHGYASKDPLSDSVDIIAQRP
CCCCCEEEHHHHHHHCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHCCCC
YLRSDLFGWLETVKPVSNNNAAPDKADGAASPAVFSHRKTVSPSDAQPVEPTAPRKMRIL
CHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCEEEEE
AAEDNKTNRLVFGKMIKALDIELQFANDGQEAVDAYPEFQPDLIFMDISMPRLDGKQATR
EECCCCCCEEHHHHHHHHHEEEEEECCCCHHHHHCCCCCCCCEEEEEECCCCCCCHHHHH
AIREIEKSTSAHVPIIALTAHAMSGDDKDILEAGLDHYMTKPLRKNEIIGKIMDFRPSDA
HHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCC
AHPCPDDMDQEAG
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]