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The map label for this gene is waaA [H]

Identifier: 110677697

GI number: 110677697

Start: 284974

End: 286221

Strand: Direct

Name: waaA [H]

Synonym: RD1_0293

Alternate gene names: 110677697

Gene position: 284974-286221 (Clockwise)

Preceding gene: 110677696

Following gene: 110677698

Centisome position: 6.89

GC content: 61.06

Gene sequence:

>1248_bases
ATGGCCCGTTCCATCGGGCTGACGGCCTATCGTGCTCTGTCCAAACGTTCAACCGAGCGGGCGTACAAGCCGCGTGGAAA
ACGGCCGCGTGGTACGCTGGTTTGGATACACGCGGGTGAGCCGCAGAACATGCTGGCGGTTCAGGATCTCGCACAGCGGT
TGTGTAACACGCGCTTTGGGGTGCATGTTCTGATCACGCTGCCTGACCCCGCATCCTACGAGGAAACCCTGCAAAGCTGG
GTGCCCCACGATCTGATCCAGATTGAACAGGTCCCGAGTGAACACCCGCATGCGGTCACATCCTTTTGGCGGCACTGGAT
GCCGGAGGTTGCGATCTGGGCATGGGGTGGTCTGCGGCCAAATCTGGTGGACCAGGTGCATCAGTCCGGATGCCCGATCG
CATTGATTGATGCGGATGCGGCCGGGTTTGACGGCAAGCGCGAGCGATGGCTGCCGGAACTGTCGCGCCAATTGCTCGAA
CCCTTTGTCGCCATCATGGTCCGCTCAAACGCCGCTGTCCAGAAATTGCAAACACTGGGGATCGAGAGTGCGCGGATCGA
CAGAAAACCCCCCTTGCAGGCGGGTGGAAATGCGTTGGCTTGCCGCGACTCGGATCTGGCGGATTTGACCGAAACCCTGC
GCGGTCGACCCGTGTGGCTGGCCAATAACATTCAGGAAGAAGAATTGCCCGTCATCCTGCAAGCCCATCGCCAGTCGCTC
AGACTGTCGCACCGTTTGCTGCTGGTGCTGCACCCGGCCCATGGGGGGCTGTCCGATGGTTTTGCCGAGAAAATCGCAAC
GGATGACCTGCGGCATGCGGATTGGACCGCAGGAGAGGACCCGGATGACGCCAGCCAGGTTTTACTGACCGAAGATCACG
GCGATCTTGGTCTGTTTTACCGCGTCGCACCTGTGACCTTCATGGGCAGTTCCCTCGTTTCGGGCTATAGCGGGCGCAAT
CCGTTCGAAGCGGCCGCGCTTGGGTCAGCGGTTCTGTATGGCCCGCATGTCAGCCGCTTCATGCCGTTTTATTCACGGCT
GGCCAAGGCGGGCGCGGCGCGCATCGTTAAGGATGTCGAAACACTGGGTGCCGCTGTCACCCAACTCATAGCGCCTGATC
AGGCCGCTGCCATGGCGCACGCAGGCTGGGATGTCGTCAGCGAAGGTGCAGATGTGACGGACCGCGTCATTGATTTGGTG
CAGGCCGCACTGGACGGGGAATTGGAGACCGCACATGCGCGCGCCTGA

Upstream 100 bases:

>100_bases
GGTGGACAATGCGCATATGCGCTATTTCATCGCGCATATTCACCGGTTGCGTGACGAGGAAACCGCCGCTTCTTCCACGG
AAGAACTGGAGTAATTCACC

Downstream 100 bases:

>100_bases
CTTCTGGTATACGCCCCCGCAGGCGCCCGCTTTGCTGGCCCGGCTCCTGCAACCGTTTGGCAAAATCTATGGCATGGCGA
CGGCCCGGCGGCTCGCGAAA

Product: 3-deoxy-D-manno-octulosonic-acid

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MARSIGLTAYRALSKRSTERAYKPRGKRPRGTLVWIHAGEPQNMLAVQDLAQRLCNTRFGVHVLITLPDPASYEETLQSW
VPHDLIQIEQVPSEHPHAVTSFWRHWMPEVAIWAWGGLRPNLVDQVHQSGCPIALIDADAAGFDGKRERWLPELSRQLLE
PFVAIMVRSNAAVQKLQTLGIESARIDRKPPLQAGGNALACRDSDLADLTETLRGRPVWLANNIQEEELPVILQAHRQSL
RLSHRLLLVLHPAHGGLSDGFAEKIATDDLRHADWTAGEDPDDASQVLLTEDHGDLGLFYRVAPVTFMGSSLVSGYSGRN
PFEAAALGSAVLYGPHVSRFMPFYSRLAKAGAARIVKDVETLGAAVTQLIAPDQAAAMAHAGWDVVSEGADVTDRVIDLV
QAALDGELETAHARA

Sequences:

>Translated_415_residues
MARSIGLTAYRALSKRSTERAYKPRGKRPRGTLVWIHAGEPQNMLAVQDLAQRLCNTRFGVHVLITLPDPASYEETLQSW
VPHDLIQIEQVPSEHPHAVTSFWRHWMPEVAIWAWGGLRPNLVDQVHQSGCPIALIDADAAGFDGKRERWLPELSRQLLE
PFVAIMVRSNAAVQKLQTLGIESARIDRKPPLQAGGNALACRDSDLADLTETLRGRPVWLANNIQEEELPVILQAHRQSL
RLSHRLLLVLHPAHGGLSDGFAEKIATDDLRHADWTAGEDPDDASQVLLTEDHGDLGLFYRVAPVTFMGSSLVSGYSGRN
PFEAAALGSAVLYGPHVSRFMPFYSRLAKAGAARIVKDVETLGAAVTQLIAPDQAAAMAHAGWDVVSEGADVTDRVIDLV
QAALDGELETAHARA
>Mature_414_residues
ARSIGLTAYRALSKRSTERAYKPRGKRPRGTLVWIHAGEPQNMLAVQDLAQRLCNTRFGVHVLITLPDPASYEETLQSWV
PHDLIQIEQVPSEHPHAVTSFWRHWMPEVAIWAWGGLRPNLVDQVHQSGCPIALIDADAAGFDGKRERWLPELSRQLLEP
FVAIMVRSNAAVQKLQTLGIESARIDRKPPLQAGGNALACRDSDLADLTETLRGRPVWLANNIQEEELPVILQAHRQSLR
LSHRLLLVLHPAHGGLSDGFAEKIATDDLRHADWTAGEDPDDASQVLLTEDHGDLGLFYRVAPVTFMGSSLVSGYSGRNP
FEAAALGSAVLYGPHVSRFMPFYSRLAKAGAARIVKDVETLGAAVTQLIAPDQAAAMAHAGWDVVSEGADVTDRVIDLVQ
AALDGELETAHARA

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers a single molecule of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=377, Percent_Identity=25.7294429708223, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 45456; Mature: 45325

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARSIGLTAYRALSKRSTERAYKPRGKRPRGTLVWIHAGEPQNMLAVQDLAQRLCNTRFG
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCC
VHVLITLPDPASYEETLQSWVPHDLIQIEQVPSEHPHAVTSFWRHWMPEVAIWAWGGLRP
EEEEEECCCCCCHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEECCCCCH
NLVDQVHQSGCPIALIDADAAGFDGKRERWLPELSRQLLEPFVAIMVRSNAAVQKLQTLG
HHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
IESARIDRKPPLQAGGNALACRDSDLADLTETLRGRPVWLANNIQEEELPVILQAHRQSL
CCHHCCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHH
RLSHRLLLVLHPAHGGLSDGFAEKIATDDLRHADWTAGEDPDDASQVLLTEDHGDLGLFY
HHCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEE
RVAPVTFMGSSLVSGYSGRNPFEAAALGSAVLYGPHVSRFMPFYSRLAKAGAARIVKDVE
EHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHEECCCHHHHHHHHHHHHHHHHHHHHHHHH
TLGAAVTQLIAPDQAAAMAHAGWDVVSEGADVTDRVIDLVQAALDGELETAHARA
HHHHHHHHHHCCCHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHCCCCCHHCCCC
>Mature Secondary Structure 
ARSIGLTAYRALSKRSTERAYKPRGKRPRGTLVWIHAGEPQNMLAVQDLAQRLCNTRFG
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCC
VHVLITLPDPASYEETLQSWVPHDLIQIEQVPSEHPHAVTSFWRHWMPEVAIWAWGGLRP
EEEEEECCCCCCHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEECCCCCH
NLVDQVHQSGCPIALIDADAAGFDGKRERWLPELSRQLLEPFVAIMVRSNAAVQKLQTLG
HHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
IESARIDRKPPLQAGGNALACRDSDLADLTETLRGRPVWLANNIQEEELPVILQAHRQSL
CCHHCCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHH
RLSHRLLLVLHPAHGGLSDGFAEKIATDDLRHADWTAGEDPDDASQVLLTEDHGDLGLFY
HHCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEE
RVAPVTFMGSSLVSGYSGRNPFEAAALGSAVLYGPHVSRFMPFYSRLAKAGAARIVKDVE
EHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHEECCCHHHHHHHHHHHHHHHHHHHHHHHH
TLGAAVTQLIAPDQAAAMAHAGWDVVSEGADVTDRVIDLVQAALDGELETAHARA
HHHHHHHHHHCCCHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952982; 7542800 [H]