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The map label for this gene is pmbA [H]

Identifier: 110677694

GI number: 110677694

Start: 282483

End: 283883

Strand: Direct

Name: pmbA [H]

Synonym: RD1_0290

Alternate gene names: 110677694

Gene position: 282483-283883 (Clockwise)

Preceding gene: 110677693

Following gene: 110677695

Centisome position: 6.83

GC content: 63.24

Gene sequence:

>1401_bases
TTGCAGAGGGGGAGCGAGGCCGCTACCTCAACCCCACCTCTAACGGAGCATATGATGACGCAGGCCCTCAGCGATCTGAA
CGAACAACTCTTGCAGGCCGCCCGGCGCGCGGGGGCTGATGATGCCGATGCGATGGCCGTTCGGGGTACGTCCCTTGGCA
TCGAAGTGCGTCACGGCGCGCTGGAACAGGCTGAACGTTCGGAAGGTATAGACATCGGTCTGCGCGTCTTTCTGGGCCGG
CGCAGCGCGATTGTCTCAGCCTCTGACACACGTTCTGATACGCTTAAAGATATGGCCGAGCGGGCCGTTGCCATGGCAAA
AGAAGCCCCCGAAGACCCCTGCGCCGGGCTTGCCAACGCCGATCAGATCACCCAAGGCTACCACATCGCCGCCTTGGAAT
TATACGACCCCACACCGGAACCATCGCCCGCCGCGCTGCAAGAGGACGCAGCCCGCGCCGAGGCCGCAGCCCTTGGCAAC
CCGCAGGTCAGTCAGGTGCAATCCGCCTCCGCCGGGTATTCATCGCGCGAGGTACATTTTGCGGCCACCAATGGATTTCG
GGGGGGCTATGCGCGCACCGACCGCGGGTTGTCCTGCGTCGCGATCTGCGGCACCGGCACCGAGATGGAGCGCGATTACG
ACAGCGACAGCCGGATTTTTCAATCGGACCTGCGCAGCCCCGAAGACATCGGCACCCGTGCCGCGGCGCGTGCCGTCGAA
CGCGCAGGTGCTCGCAAACCGCCCACCGGCAGCTATCCGGTCCTGTTTGACGAGCGTGTTTCCTCAACGCTGATCGGTCA
CTTGCTGATGGCGGCAAATGGGTCCTCCGTGGCGCGGGGTGCGTCCTGGCTGCGTGACGCAAAAGGCGAGCAGGTTCTGC
CATCAGGCATCAGTCTGCATGAGCTGCCTCACAGGCCGCGCACCTCCGGCTCCCGCCCCTTTGATGCCGAAGGATTGCCA
ACCAAGGCGCGTGACATCGTGGCAGATGGTGTGCTGACCGGCTGGACCATGGACCTTGCAACGGCGCGCAAACTGGGCAT
GTCCTCGACAGGGAACGCCGCGCGCAGTGCCTCTTCGGGGCCAAGCCCGACATCATGGAACGTCACCCTCACCCAAGGGG
ATCAAAGCCGCGCAGACCTGATTGCTGACATGGGTACGGGCCTGCTCGTCACTTCGATGATCGGGTCGACGATCAATTCC
AACACCGGGGATTATTCCCGTGGGGCCGCAGGCATCTGGATCGAAAATGGTGAGATGACATATGCGGTCAACGAATGCAC
GATTGCGGGCAACCTGCGGGACATGCTGCTGAACATTGTCCCCGCAAACGACGCGCGCGCGCATCTGAGCCGGGTCGTGC
CCTCGCTTCTTGTACCGGGGATGACACTTGCAGGCGTCTGA

Upstream 100 bases:

>100_bases
TACGCTCAACTCAAAGATCGAAATCAACGATCAGGGCCATGTGATGGCAAACGGGCAGCGCATCCAGTAACCGCGGCGAC
GCAGTTTTTGAGCGCAGCCC

Downstream 100 bases:

>100_bases
CCTTGATCTGATCACGGACGCAATCGTCATGGCGGGCCGGGTTGCGACCAGCTTCACCGGCCAGAGCACGCAAAGCTGGG
ACAAGCCCGGCGGTGCAGGC

Product: PmbA protein, putative

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MQRGSEAATSTPPLTEHMMTQALSDLNEQLLQAARRAGADDADAMAVRGTSLGIEVRHGALEQAERSEGIDIGLRVFLGR
RSAIVSASDTRSDTLKDMAERAVAMAKEAPEDPCAGLANADQITQGYHIAALELYDPTPEPSPAALQEDAARAEAAALGN
PQVSQVQSASAGYSSREVHFAATNGFRGGYARTDRGLSCVAICGTGTEMERDYDSDSRIFQSDLRSPEDIGTRAAARAVE
RAGARKPPTGSYPVLFDERVSSTLIGHLLMAANGSSVARGASWLRDAKGEQVLPSGISLHELPHRPRTSGSRPFDAEGLP
TKARDIVADGVLTGWTMDLATARKLGMSSTGNAARSASSGPSPTSWNVTLTQGDQSRADLIADMGTGLLVTSMIGSTINS
NTGDYSRGAAGIWIENGEMTYAVNECTIAGNLRDMLLNIVPANDARAHLSRVVPSLLVPGMTLAGV

Sequences:

>Translated_466_residues
MQRGSEAATSTPPLTEHMMTQALSDLNEQLLQAARRAGADDADAMAVRGTSLGIEVRHGALEQAERSEGIDIGLRVFLGR
RSAIVSASDTRSDTLKDMAERAVAMAKEAPEDPCAGLANADQITQGYHIAALELYDPTPEPSPAALQEDAARAEAAALGN
PQVSQVQSASAGYSSREVHFAATNGFRGGYARTDRGLSCVAICGTGTEMERDYDSDSRIFQSDLRSPEDIGTRAAARAVE
RAGARKPPTGSYPVLFDERVSSTLIGHLLMAANGSSVARGASWLRDAKGEQVLPSGISLHELPHRPRTSGSRPFDAEGLP
TKARDIVADGVLTGWTMDLATARKLGMSSTGNAARSASSGPSPTSWNVTLTQGDQSRADLIADMGTGLLVTSMIGSTINS
NTGDYSRGAAGIWIENGEMTYAVNECTIAGNLRDMLLNIVPANDARAHLSRVVPSLLVPGMTLAGV
>Mature_466_residues
MQRGSEAATSTPPLTEHMMTQALSDLNEQLLQAARRAGADDADAMAVRGTSLGIEVRHGALEQAERSEGIDIGLRVFLGR
RSAIVSASDTRSDTLKDMAERAVAMAKEAPEDPCAGLANADQITQGYHIAALELYDPTPEPSPAALQEDAARAEAAALGN
PQVSQVQSASAGYSSREVHFAATNGFRGGYARTDRGLSCVAICGTGTEMERDYDSDSRIFQSDLRSPEDIGTRAAARAVE
RAGARKPPTGSYPVLFDERVSSTLIGHLLMAANGSSVARGASWLRDAKGEQVLPSGISLHELPHRPRTSGSRPFDAEGLP
TKARDIVADGVLTGWTMDLATARKLGMSSTGNAARSASSGPSPTSWNVTLTQGDQSRADLIADMGTGLLVTSMIGSTINS
NTGDYSRGAAGIWIENGEMTYAVNECTIAGNLRDMLLNIVPANDARAHLSRVVPSLLVPGMTLAGV

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=429, Percent_Identity=36.3636363636364, Blast_Score=253, Evalue=1e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48935; Mature: 48935

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRGSEAATSTPPLTEHMMTQALSDLNEQLLQAARRAGADDADAMAVRGTSLGIEVRHGA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEECCCH
LEQAERSEGIDIGLRVFLGRRSAIVSASDTRSDTLKDMAERAVAMAKEAPEDPCAGLANA
HHHHHHHCCCEEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
DQITQGYHIAALELYDPTPEPSPAALQEDAARAEAAALGNPQVSQVQSASAGYSSREVHF
HHHHCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEE
AATNGFRGGYARTDRGLSCVAICGTGTEMERDYDSDSRIFQSDLRSPEDIGTRAAARAVE
EECCCCCCCCCCCCCCCEEEEEECCCCHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHH
RAGARKPPTGSYPVLFDERVSSTLIGHLLMAANGSSVARGASWLRDAKGEQVLPSGISLH
HCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHH
ELPHRPRTSGSRPFDAEGLPTKARDIVADGVLTGWTMDLATARKLGMSSTGNAARSASSG
HCCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHCCHHHHHHHHHHCCCCCCCHHHCCCCC
PSPTSWNVTLTQGDQSRADLIADMGTGLLVTSMIGSTINSNTGDYSRGAAGIWIENGEMT
CCCCEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCEEE
YAVNECTIAGNLRDMLLNIVPANDARAHLSRVVPSLLVPGMTLAGV
EEEECEEEECCHHHHHHEECCCCHHHHHHHHHHHHHHCCCCHHCCC
>Mature Secondary Structure
MQRGSEAATSTPPLTEHMMTQALSDLNEQLLQAARRAGADDADAMAVRGTSLGIEVRHGA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEECCCH
LEQAERSEGIDIGLRVFLGRRSAIVSASDTRSDTLKDMAERAVAMAKEAPEDPCAGLANA
HHHHHHHCCCEEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
DQITQGYHIAALELYDPTPEPSPAALQEDAARAEAAALGNPQVSQVQSASAGYSSREVHF
HHHHCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEE
AATNGFRGGYARTDRGLSCVAICGTGTEMERDYDSDSRIFQSDLRSPEDIGTRAAARAVE
EECCCCCCCCCCCCCCCEEEEEECCCCHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHH
RAGARKPPTGSYPVLFDERVSSTLIGHLLMAANGSSVARGASWLRDAKGEQVLPSGISLH
HCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHH
ELPHRPRTSGSRPFDAEGLPTKARDIVADGVLTGWTMDLATARKLGMSSTGNAARSASSG
HCCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHCCHHHHHHHHHHCCCCCCCHHHCCCCC
PSPTSWNVTLTQGDQSRADLIADMGTGLLVTSMIGSTINSNTGDYSRGAAGIWIENGEMT
CCCCEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCEEE
YAVNECTIAGNLRDMLLNIVPANDARAHLSRVVPSLLVPGMTLAGV
EEEECEEEECCHHHHHHEECCCCHHHHHHHHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]