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The map label for this gene is dapE [H]

Identifier: 110677505

GI number: 110677505

Start: 92563

End: 93852

Strand: Direct

Name: dapE [H]

Synonym: RD1_0090

Alternate gene names: 110677505

Gene position: 92563-93852 (Clockwise)

Preceding gene: 110677504

Following gene: 110677508

Centisome position: 2.24

GC content: 59.84

Gene sequence:

>1290_bases
ATGCAACCAGACCTCGCCGCAGCCATAGAAAACAGACGAGACGACCTGATCGCCCTCACGCAGGCGCTGATCCGGATCCC
GACGCTGAACCCGCCGGGCTGTGAGTATCACACAATCTGCCAGTTGATCGCATCCCGTCTTGAGGCGCGCGGCTTTGCCA
TCGAGATGATCCGCGCCAGGGGGACACCCGGCGACAGTGACACATACCCAAGGTGGAACGTTGTAGCCCGGCATGATGGG
GCCACACAGGGTGAATGCGTGCATTTTAACAGCCATATCGACGTGGTCGAGGTGGGGCAGGGCTGGACCCATGACCCCTT
TGCCGGCACGCTGCAGGATGGTCGGATTTATGGCCGCGGTGCTTGTGACATGAAGGGGGGGCTTGCTGCGTCGATCATCG
CGGTTGAAGCCTTCATCGACACCTACCCCGATTACGCCGGTGCCATCGAAATTTCAGGCACGGCGGATGAGGAATCGGGT
GGATATGGCGGTGTCGCCTATCTTGCAGAGCGGGGGTATTTCGACCCGCAGCGTGTGCAGCATGCGATCATCCCGGAACC
TTTGAACAAGGACCGCATCTGCCTTGGGCACCGGGGCGGCTGGTGGGCAGAGATCGAAACCCATGGTGAAATTGCGCATG
GCTCCATGCCTTTCCTGGGCGATTGTGCCGTGCGCCATATGGGTGCGGTGCTGTCTGAATTCGAAGACAAGCTGTTCCCC
GCCATGGCCCTGCGTCGCACCGATATGCCCGTTGTGCCCGATGGCGCGCGGCAATCCACGATGAACATCAATTCGATCCA
TGGCGGACAGGCCGAACAGGATGCCGATTTTGACGGGCTGCCCGCGCATTGCGTACCGGACAGTTGCCGCATCGTGATTG
ACCGCCGTTTCCTGCTGGAAGAACCGCTGGATCAGGTCCGTGCCGAAGTCACCGGCCTGCTTGAAACCCTTAAACAAACA
CGCCCGGATTTCGAATATACGCTGACCGAGATCAATTCTGTGCTGCCCTCGATGACCGACCGGGACGCGCCGATTGTTCG
CGTTGTGGCACAGGCCATCGAAGAGGTGATCGGCAACCCCCCGGACTACGTGGCCTCCCCCGGCACCTATGATCAGAAAC
ACATCGACCGGATCGGCAAGCTGAAAAACTGTATCGCCTATGGGCCGGGTATTCTGGAGCTTGCGCATAAGCCGGATGAA
TATGTCGGCGTCGATGACATGATCGACAGCGCGCAGGTGATGGCGCAATCGCTGGTCACACTGTTGACGGGCAAGGCCCA
GAGCGTCTGA

Upstream 100 bases:

>100_bases
ACCCTCACCGAGGTCTCGGCCTTTCTGGCCGCCATGAAAGATATCGCTGCTCGGGCCAAGTAGGCTTGCCATTTGAACAG
CCACGGCCAAGACTAAGGCC

Downstream 100 bases:

>100_bases
CGGTATATCTGAAACATCACGTTACGCTTTGAAATCTTTGATTTCACGCAGCGTTGGCGTGATTGAGGCTTTTCGCATCG
CGCTTTAGCGCGCCACCGGC

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MQPDLAAAIENRRDDLIALTQALIRIPTLNPPGCEYHTICQLIASRLEARGFAIEMIRARGTPGDSDTYPRWNVVARHDG
ATQGECVHFNSHIDVVEVGQGWTHDPFAGTLQDGRIYGRGACDMKGGLAASIIAVEAFIDTYPDYAGAIEISGTADEESG
GYGGVAYLAERGYFDPQRVQHAIIPEPLNKDRICLGHRGGWWAEIETHGEIAHGSMPFLGDCAVRHMGAVLSEFEDKLFP
AMALRRTDMPVVPDGARQSTMNINSIHGGQAEQDADFDGLPAHCVPDSCRIVIDRRFLLEEPLDQVRAEVTGLLETLKQT
RPDFEYTLTEINSVLPSMTDRDAPIVRVVAQAIEEVIGNPPDYVASPGTYDQKHIDRIGKLKNCIAYGPGILELAHKPDE
YVGVDDMIDSAQVMAQSLVTLLTGKAQSV

Sequences:

>Translated_429_residues
MQPDLAAAIENRRDDLIALTQALIRIPTLNPPGCEYHTICQLIASRLEARGFAIEMIRARGTPGDSDTYPRWNVVARHDG
ATQGECVHFNSHIDVVEVGQGWTHDPFAGTLQDGRIYGRGACDMKGGLAASIIAVEAFIDTYPDYAGAIEISGTADEESG
GYGGVAYLAERGYFDPQRVQHAIIPEPLNKDRICLGHRGGWWAEIETHGEIAHGSMPFLGDCAVRHMGAVLSEFEDKLFP
AMALRRTDMPVVPDGARQSTMNINSIHGGQAEQDADFDGLPAHCVPDSCRIVIDRRFLLEEPLDQVRAEVTGLLETLKQT
RPDFEYTLTEINSVLPSMTDRDAPIVRVVAQAIEEVIGNPPDYVASPGTYDQKHIDRIGKLKNCIAYGPGILELAHKPDE
YVGVDDMIDSAQVMAQSLVTLLTGKAQSV
>Mature_429_residues
MQPDLAAAIENRRDDLIALTQALIRIPTLNPPGCEYHTICQLIASRLEARGFAIEMIRARGTPGDSDTYPRWNVVARHDG
ATQGECVHFNSHIDVVEVGQGWTHDPFAGTLQDGRIYGRGACDMKGGLAASIIAVEAFIDTYPDYAGAIEISGTADEESG
GYGGVAYLAERGYFDPQRVQHAIIPEPLNKDRICLGHRGGWWAEIETHGEIAHGSMPFLGDCAVRHMGAVLSEFEDKLFP
AMALRRTDMPVVPDGARQSTMNINSIHGGQAEQDADFDGLPAHCVPDSCRIVIDRRFLLEEPLDQVRAEVTGLLETLKQT
RPDFEYTLTEINSVLPSMTDRDAPIVRVVAQAIEEVIGNPPDYVASPGTYDQKHIDRIGKLKNCIAYGPGILELAHKPDE
YVGVDDMIDSAQVMAQSLVTLLTGKAQSV

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501901, Length=191, Percent_Identity=29.8429319371728, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1788816, Length=407, Percent_Identity=28.2555282555283, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1789236, Length=350, Percent_Identity=25.7142857142857, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1790395, Length=419, Percent_Identity=24.582338902148, Blast_Score=78, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24649206, Length=421, Percent_Identity=23.0403800475059, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21356353, Length=343, Percent_Identity=24.4897959183673, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 46842; Mature: 46842

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPDLAAAIENRRDDLIALTQALIRIPTLNPPGCEYHTICQLIASRLEARGFAIEMIRAR
CCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEHHHC
GTPGDSDTYPRWNVVARHDGATQGECVHFNSHIDVVEVGQGWTHDPFAGTLQDGRIYGRG
CCCCCCCCCCCCEEEEECCCCCCCCEEEECCCCCEEECCCCCCCCCCCCCCCCCEEEECC
ACDMKGGLAASIIAVEAFIDTYPDYAGAIEISGTADEESGGYGGVAYLAERGYFDPQRVQ
CCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCHHHHHHHCCCCCHHHHH
HAIIPEPLNKDRICLGHRGGWWAEIETHGEIAHGSMPFLGDCAVRHMGAVLSEFEDKLFP
HHCCCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AMALRRTDMPVVPDGARQSTMNINSIHGGQAEQDADFDGLPAHCVPDSCRIVIDRRFLLE
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHH
EPLDQVRAEVTGLLETLKQTRPDFEYTLTEINSVLPSMTDRDAPIVRVVAQAIEEVIGNP
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCC
PDYVASPGTYDQKHIDRIGKLKNCIAYGPGILELAHKPDEYVGVDDMIDSAQVMAQSLVT
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
LLTGKAQSV
HHHCCCCCC
>Mature Secondary Structure
MQPDLAAAIENRRDDLIALTQALIRIPTLNPPGCEYHTICQLIASRLEARGFAIEMIRAR
CCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEHHHC
GTPGDSDTYPRWNVVARHDGATQGECVHFNSHIDVVEVGQGWTHDPFAGTLQDGRIYGRG
CCCCCCCCCCCCEEEEECCCCCCCCEEEECCCCCEEECCCCCCCCCCCCCCCCCEEEECC
ACDMKGGLAASIIAVEAFIDTYPDYAGAIEISGTADEESGGYGGVAYLAERGYFDPQRVQ
CCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCHHHHHHHCCCCCHHHHH
HAIIPEPLNKDRICLGHRGGWWAEIETHGEIAHGSMPFLGDCAVRHMGAVLSEFEDKLFP
HHCCCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AMALRRTDMPVVPDGARQSTMNINSIHGGQAEQDADFDGLPAHCVPDSCRIVIDRRFLLE
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHH
EPLDQVRAEVTGLLETLKQTRPDFEYTLTEINSVLPSMTDRDAPIVRVVAQAIEEVIGNP
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCC
PDYVASPGTYDQKHIDRIGKLKNCIAYGPGILELAHKPDEYVGVDDMIDSAQVMAQSLVT
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
LLTGKAQSV
HHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA