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The map label for this gene is aiiA [H]

Identifier: 110677495

GI number: 110677495

Start: 83827

End: 84579

Strand: Direct

Name: aiiA [H]

Synonym: RD1_0080

Alternate gene names: 110677495

Gene position: 83827-84579 (Clockwise)

Preceding gene: 110677493

Following gene: 110677498

Centisome position: 2.03

GC content: 59.36

Gene sequence:

>753_bases
ATGCAACCCCTGCTTCACGGTCGGCCCGCGCGGTTGGCGATCCTTGATTATGGGTTGTTTCAGGTACATGAGGACGGCCG
CATCATCGGCATTTGTGGCTATCTGATCCAGACGACAGCCGGTGAAAACGTGCTGATTGATACAGGCTTTCCTGCCAAAT
ACGGCGTGGATCACGCGGCTGCGACCGAAGAAGACGCTCTGGGTGACTTTGGTCAGGTTCTGTCATTGACCGAAACAAAC
CTCGCCCCGGCGCAGCTTGCCCTGCTTGGTCTGGCGCGCAGTGACATCGACCTGATGATCCAAAGCCACACGCATATTGA
CCATGTGGGCTTCATCGGGGGCTTTCCGGGAGTGCCCATCTTGATTGCCGCAACTGAACGCGCCCTGCCCCGCCCGCTCT
ACTGGTCGGGGAAACAGGCGCTGGAATGGCCTGACGCAGACTATATCGAGTTGCAGAGCGACACCAGGTTGGGCCCCGGA
TTTGACGTGCTGCTGGTGCCGGGCCATGCCCCGGGCCAGCTTGCCTTTGTCTTGGAGTTGCCGCACACCGGCGCGGTGGT
TCTGACCTCTGATGCGATCAGCCGACCCTCAGAGCTCGATACCGATTTTGCCGGCGCATGGGATGCGCCACTTGCCCGTC
AGCACGCAAACCGGTTGATGAGGCGGGCACAGGACGACAAGGCGATGGTGATATATGGCCACTGCCCCGCACAATGGTCA
GAACTGCGAAAAGCACCAAACTGGTATGATTAG

Upstream 100 bases:

>100_bases
GCGTCGATTCCAACAGATATTCAAACAGCCTGTGTATCTTGACTTTCATCGGCTCCATCCGCTCTAGTCCACCGGCAACC
AAAGAGGCCTTGGCGTATTT

Downstream 100 bases:

>100_bases
CGCAGGTGGCGCCGCTGGTTGAGAATGATGACCTTCCCGCTTGAAACATCGCCCGCCGGGGAAACCAGAAAGCCGGGCCA
AGCGGCGATTTCAGCAGGGT

Product: hypothetical protein

Products: NA

Alternate protein names: AHL-lactonase AiiA [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MQPLLHGRPARLAILDYGLFQVHEDGRIIGICGYLIQTTAGENVLIDTGFPAKYGVDHAAATEEDALGDFGQVLSLTETN
LAPAQLALLGLARSDIDLMIQSHTHIDHVGFIGGFPGVPILIAATERALPRPLYWSGKQALEWPDADYIELQSDTRLGPG
FDVLLVPGHAPGQLAFVLELPHTGAVVLTSDAISRPSELDTDFAGAWDAPLARQHANRLMRRAQDDKAMVIYGHCPAQWS
ELRKAPNWYD

Sequences:

>Translated_250_residues
MQPLLHGRPARLAILDYGLFQVHEDGRIIGICGYLIQTTAGENVLIDTGFPAKYGVDHAAATEEDALGDFGQVLSLTETN
LAPAQLALLGLARSDIDLMIQSHTHIDHVGFIGGFPGVPILIAATERALPRPLYWSGKQALEWPDADYIELQSDTRLGPG
FDVLLVPGHAPGQLAFVLELPHTGAVVLTSDAISRPSELDTDFAGAWDAPLARQHANRLMRRAQDDKAMVIYGHCPAQWS
ELRKAPNWYD
>Mature_250_residues
MQPLLHGRPARLAILDYGLFQVHEDGRIIGICGYLIQTTAGENVLIDTGFPAKYGVDHAAATEEDALGDFGQVLSLTETN
LAPAQLALLGLARSDIDLMIQSHTHIDHVGFIGGFPGVPILIAATERALPRPLYWSGKQALEWPDADYIELQSDTRLGPG
FDVLLVPGHAPGQLAFVLELPHTGAVVLTSDAISRPSELDTDFAGAWDAPLARQHANRLMRRAQDDKAMVIYGHCPAQWS
ELRKAPNWYD

Specific function: Catalyzes hydrolysis of N-hexanoyl-(S)-homoserine lactone, but not the R-enantiomer. Hydrolyzes short- and long- chain N-acyl homoserine lactones with or without 3-oxo substitution at C3, has maximum activity on C10-AHL [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.1.81 [H]

Molecular weight: Translated: 27231; Mature: 27231

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPLLHGRPARLAILDYGLFQVHEDGRIIGICGYLIQTTAGENVLIDTGFPAKYGVDHAA
CCCCCCCCCCEEEEEECCEEEECCCCCEEEEEEEHHHCCCCCEEEEECCCCHHHCCCCCC
ATEEDALGDFGQVLSLTETNLAPAQLALLGLARSDIDLMIQSHTHIDHVGFIGGFPGVPI
CCCHHHHCCHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCE
LIAATERALPRPLYWSGKQALEWPDADYIELQSDTRLGPGFDVLLVPGHAPGQLAFVLEL
EEEECCCCCCCCEEECCCCCCCCCCCCEEEECCCCCCCCCEEEEEECCCCCCCEEEEEEC
PHTGAVVLTSDAISRPSELDTDFAGAWDAPLARQHANRLMRRAQDDKAMVIYGHCPAQWS
CCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHH
ELRKAPNWYD
HHHCCCCCCC
>Mature Secondary Structure
MQPLLHGRPARLAILDYGLFQVHEDGRIIGICGYLIQTTAGENVLIDTGFPAKYGVDHAA
CCCCCCCCCCEEEEEECCEEEECCCCCEEEEEEEHHHCCCCCEEEEECCCCHHHCCCCCC
ATEEDALGDFGQVLSLTETNLAPAQLALLGLARSDIDLMIQSHTHIDHVGFIGGFPGVPI
CCCHHHHCCHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCE
LIAATERALPRPLYWSGKQALEWPDADYIELQSDTRLGPGFDVLLVPGHAPGQLAFVLEL
EEEECCCCCCCCEEECCCCCCCCCCCCEEEECCCCCCCCCEEEEEECCCCCCCEEEEEEC
PHTGAVVLTSDAISRPSELDTDFAGAWDAPLARQHANRLMRRAQDDKAMVIYGHCPAQWS
CCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHH
ELRKAPNWYD
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11916693; 12147491 [H]