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The map label for this gene is vanB [H]

Identifier: 110677424

GI number: 110677424

Start: 3553

End: 4539

Strand: Direct

Name: vanB [H]

Synonym: RD1_0005

Alternate gene names: 110677424

Gene position: 3553-4539 (Clockwise)

Preceding gene: 110677423

Following gene: 110677425

Centisome position: 0.09

GC content: 59.57

Gene sequence:

>987_bases
ATGAGCGCCGCCCCCCAGAAAGCCAAAGCCGGTGCCGAAAAGATCGAGGTCGAAGTCAGCGCCATTGTCCCCGTCAACGA
ACTGGTCTCACGGTTCGAATTCAAACGCCGCGACGGGCAGCCGTTCCCACCTTTTTCTGCGGGCGCACATACCGTCGTTG
AAATGAATGACAATGGCCGGATGCGTCTCAACCCCTATTCGCTCATGTCTGACCCGGCGGATACGTCGATCTATGCGATC
TCCGTGCGCCGCGACGATGAGGGACGGGGCGGGTCACTTTTCATGCACCGGCACGTCAAGGTCGGCGATCCGATGACGAT
CACCTATCCGGTCAACCTCTTTCCGCTTGATCTGCGCGGGCACAAACATGTGTTTCTTGCAGGCGGGATCGGCATCACGC
CCTTCCTGTCGATGATTGCCACGCTCGAACGCATGGGCGGGCGTTGGGAAATGCACTACGCCTGCCGGAGCAAAGCACTG
GGGTCTTACGTGGACGAACTGACAGGACGCCACCCGGACAAGGTGCATGTCTATTGCGATGATCAGGACGAGGCGATCCC
CCTGGAAAGCCTGCTCGATGGTCAGCCCCTGGGCACACATGTCTATGTCTGCGGCCCGGCGGGAATGATCAACTGGGTGC
ACAGCACCGCCGAAGCGCATGGATGGCCCAAGGAACACGTACACTCCGAAGAATTCCTTGCGCCGCAACCGGGCAAACCT
TTTGAGGTACGCTTGTGCAAATCCGACATCGTGATTCAGGTGGGTGAGCACGAAAGCCTGCTGGAAGCGATCGAGCGTTG
CGGCGTTCAGGCGCCGTTCCTCTGCCGCGGCGGAGCTTGCGGGCAGTGCGAAACCGATGTGGTCGAGGCCGATGGCGAAT
TCATCCACCGCGACCACTGGCTGGAAGAAGAAGAACACGCCAGCGGCAAGAAAATCATGCCATGCGTCAGCCGCTTTGTC
GGCAAAACGCTGGTTCTGGATCGATAG

Upstream 100 bases:

>100_bases
TCGTTCGGGATCATTATTCACGCAGACTTGCCGCCTTTCAGGGCGTGTGCATTGATGCCGAAGATCCCGGAAACATCCCC
GAACCCGTGGAGCTGTACAA

Downstream 100 bases:

>100_bases
GGAGGTCCTTTATGACGATACAATTCAACACCGAAACGTTCCGCGACGACTATTCCTTCCGCAACTCGGACTGGGCGATC
AAACGCTTCCCGTTTCCGTT

Product: vanillate O-demethylase oxidoreductase, putative

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MSAAPQKAKAGAEKIEVEVSAIVPVNELVSRFEFKRRDGQPFPPFSAGAHTVVEMNDNGRMRLNPYSLMSDPADTSIYAI
SVRRDDEGRGGSLFMHRHVKVGDPMTITYPVNLFPLDLRGHKHVFLAGGIGITPFLSMIATLERMGGRWEMHYACRSKAL
GSYVDELTGRHPDKVHVYCDDQDEAIPLESLLDGQPLGTHVYVCGPAGMINWVHSTAEAHGWPKEHVHSEEFLAPQPGKP
FEVRLCKSDIVIQVGEHESLLEAIERCGVQAPFLCRGGACGQCETDVVEADGEFIHRDHWLEEEEHASGKKIMPCVSRFV
GKTLVLDR

Sequences:

>Translated_328_residues
MSAAPQKAKAGAEKIEVEVSAIVPVNELVSRFEFKRRDGQPFPPFSAGAHTVVEMNDNGRMRLNPYSLMSDPADTSIYAI
SVRRDDEGRGGSLFMHRHVKVGDPMTITYPVNLFPLDLRGHKHVFLAGGIGITPFLSMIATLERMGGRWEMHYACRSKAL
GSYVDELTGRHPDKVHVYCDDQDEAIPLESLLDGQPLGTHVYVCGPAGMINWVHSTAEAHGWPKEHVHSEEFLAPQPGKP
FEVRLCKSDIVIQVGEHESLLEAIERCGVQAPFLCRGGACGQCETDVVEADGEFIHRDHWLEEEEHASGKKIMPCVSRFV
GKTLVLDR
>Mature_327_residues
SAAPQKAKAGAEKIEVEVSAIVPVNELVSRFEFKRRDGQPFPPFSAGAHTVVEMNDNGRMRLNPYSLMSDPADTSIYAIS
VRRDDEGRGGSLFMHRHVKVGDPMTITYPVNLFPLDLRGHKHVFLAGGIGITPFLSMIATLERMGGRWEMHYACRSKALG
SYVDELTGRHPDKVHVYCDDQDEAIPLESLLDGQPLGTHVYVCGPAGMINWVHSTAEAHGWPKEHVHSEEFLAPQPGKPF
EVRLCKSDIVIQVGEHESLLEAIERCGVQAPFLCRGGACGQCETDVVEADGEFIHRDHWLEEEEHASGKKIMPCVSRFVG
KTLVLDR

Specific function: Unknown

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=319, Percent_Identity=41.692789968652, Blast_Score=241, Evalue=6e-65,
Organism=Escherichia coli, GI1787658, Length=320, Percent_Identity=23.4375, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788903, Length=239, Percent_Identity=24.6861924686192, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 36364; Mature: 36233

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAPQKAKAGAEKIEVEVSAIVPVNELVSRFEFKRRDGQPFPPFSAGAHTVVEMNDNGR
CCCCCCHHHCCCEEEEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCC
MRLNPYSLMSDPADTSIYAISVRRDDEGRGGSLFMHRHVKVGDPMTITYPVNLFPLDLRG
EEECCHHHCCCCCCCEEEEEEEEECCCCCCCCEEEEEEEECCCCEEEEEEEEEEEEECCC
HKHVFLAGGIGITPFLSMIATLERMGGRWEMHYACRSKALGSYVDELTGRHPDKVHVYCD
CEEEEEECCCCHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEEC
DQDEAIPLESLLDGQPLGTHVYVCGPAGMINWVHSTAEAHGWPKEHVHSEEFLAPQPGKP
CCCCCCCHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCC
FEVRLCKSDIVIQVGEHESLLEAIERCGVQAPFLCRGGACGQCETDVVEADGEFIHRDHW
EEEEEECCCEEEEECCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCEEECCCCEEHHHCC
LEEEEHASGKKIMPCVSRFVGKTLVLDR
CCHHHHCCCCHHHHHHHHHHCCEEECCC
>Mature Secondary Structure 
SAAPQKAKAGAEKIEVEVSAIVPVNELVSRFEFKRRDGQPFPPFSAGAHTVVEMNDNGR
CCCCCHHHCCCEEEEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCC
MRLNPYSLMSDPADTSIYAISVRRDDEGRGGSLFMHRHVKVGDPMTITYPVNLFPLDLRG
EEECCHHHCCCCCCCEEEEEEEEECCCCCCCCEEEEEEEECCCCEEEEEEEEEEEEECCC
HKHVFLAGGIGITPFLSMIATLERMGGRWEMHYACRSKALGSYVDELTGRHPDKVHVYCD
CEEEEEECCCCHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEEC
DQDEAIPLESLLDGQPLGTHVYVCGPAGMINWVHSTAEAHGWPKEHVHSEEFLAPQPGKP
CCCCCCCHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCC
FEVRLCKSDIVIQVGEHESLLEAIERCGVQAPFLCRGGACGQCETDVVEADGEFIHRDHW
EEEEEECCCEEEEECCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCEEECCCCEEHHHCC
LEEEEHASGKKIMPCVSRFVGKTLVLDR
CCHHHHCCCCHHHHHHHHHHCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]