Click here to switch to the map view.

The map label for this gene is pcaB [H]

Identifier: 110635948

GI number: 110635948

Start: 3908436

End: 3909806

Strand: Reverse

Name: pcaB [H]

Synonym: Meso_3622

Alternate gene names: 110635948

Gene position: 3909806-3908436 (Counterclockwise)

Preceding gene: 110635949

Following gene: 110635947

Centisome position: 88.61

GC content: 62.8

Gene sequence:

>1371_bases
ATGAGAATATCGATGGCGACCTTCTTTCCTCAATCAGTGACGGCACTCGACTCCCCGCTTTACGGGCGTAGCTTCGCCGA
TGACAAAATGCGCGAGCTCTTTTCCGCGCAAAGCTTCATTTCTCGCTGTGTAGAAACGGAGGTCGCCCTCGCCAGGGCGC
AGGCGCGCCTCGGCATTATTCCCGAAGACGCAGCGGCGGGCATCACCGCCGCTGCCAGAACCTTCGCGCCTGAGATGGAA
CGCCTGCGCGACGATACCGAGATCGTCGGCTATCCAATCCTGCCGCTGGTCGAACAGCTATCCGCCCATGCGGGCGAAGC
CGGAAAATACCTTCACTGGGGCGCGACTACGCAGGACATCATGGACACCGCCACCGTCCTGCAAATTCGGGATGGGTTAG
CTCTCATTAGCCGGCGCATCGAAAGCGTGCGCAAGGCATTGGCGGCGCTGGCGCGTAATCATCGCGACACGCCGATGGCC
GGCCGCACACATCTTCAACACGCATTGCCGGTCACTTTTGGCTACAAGGCAGCCGTGTGGCTTTCCGCCTTCGACAGGCA
TGCCGCGCGCCTTGAGGAAATCAGCCCCCGCGTGCTGGTGGTCGAATTCTCCGGTGCGTCCGGAACGCTCGCCTCGCTCG
GAACGCGCGGGCTGGACGTCCAGCGCGAACTGGCGCGCGAGCTCAATCTCGGCGTTCCTTCGATCACCTGGCATTCCGCG
CGCGACGCGGTCGCGGAAACCGTTCAGTTCCTGGCTCTCGTTTCCGGCAGCCTCGGCAAGCTCGCGATGGACATTTCTAT
CATGATGACGACGGAACTCGGTGAGGTCGCCGAGCCCTTTGTGCGTCATCGCGGCGCCAGCAGCACCATGCCGCAAAAAC
AGAACCCCGTTTCCTGCGAGCTCATCCTCGCCGGTGCGCGGATCGTGCGCAATCACGCCACGTCGATGCTCGACGCGATG
ATCCACGACTTCGAGCGCGCGACCGGGCCGTGGCACCTGGAATGGTCCGCCGTCCCCGAAGGCTTTGCAGTCGCATCGGG
GATACTCTATCAGGCCGAATTCATGCTGGGCGGCCTCCAGGTCTTCCCGGATCGGATGCGCGAAAATCTTGATCATTCAC
GCGGGCTGATCGTGGCGGAAGCCGTCATGATGGCGCTTGCGCCCCATACCGGGCGTAAGGAAGCGCACGATATCGTCTAT
CTCGGCTGCCGCCGTGCGGTGGAGGACAAGACCGGTCTTTTCGAGGTGCTGCGCACCATGCCGGAAGTGGCCAAGCCGCT
CGGCGAAGAGGCGCTCAGGGATCTGACTGATCCCCGCAACTATCTCGGCAGCGCCGGCGCTATGGTCGACAATGTGCTCG
GCGGCCGATGA

Upstream 100 bases:

>100_bases
CCGCGAACGACTTCATGCCCTATCACCCAGGTGCCGTACGCTACTACCGCGAGCAGGGGATCGAGCTCCCCGAAGCAGTC
ATCCCCGCTGAAATGGCTGA

Downstream 100 bases:

>100_bases
CTATTCGCTCGGAATCGGACGCAAACGGTCCCATTGAAATTCCAGCGGATCGCTACTGGGGAGCGCAGACCGCCCGCGCG
CTGAGGCTGTTCCCGCAGGA

Product: fumarate lyase

Products: NA

Alternate protein names: 3-carboxymuconate lactonizing enzyme; CMLE [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MRISMATFFPQSVTALDSPLYGRSFADDKMRELFSAQSFISRCVETEVALARAQARLGIIPEDAAAGITAAARTFAPEME
RLRDDTEIVGYPILPLVEQLSAHAGEAGKYLHWGATTQDIMDTATVLQIRDGLALISRRIESVRKALAALARNHRDTPMA
GRTHLQHALPVTFGYKAAVWLSAFDRHAARLEEISPRVLVVEFSGASGTLASLGTRGLDVQRELARELNLGVPSITWHSA
RDAVAETVQFLALVSGSLGKLAMDISIMMTTELGEVAEPFVRHRGASSTMPQKQNPVSCELILAGARIVRNHATSMLDAM
IHDFERATGPWHLEWSAVPEGFAVASGILYQAEFMLGGLQVFPDRMRENLDHSRGLIVAEAVMMALAPHTGRKEAHDIVY
LGCRRAVEDKTGLFEVLRTMPEVAKPLGEEALRDLTDPRNYLGSAGAMVDNVLGGR

Sequences:

>Translated_456_residues
MRISMATFFPQSVTALDSPLYGRSFADDKMRELFSAQSFISRCVETEVALARAQARLGIIPEDAAAGITAAARTFAPEME
RLRDDTEIVGYPILPLVEQLSAHAGEAGKYLHWGATTQDIMDTATVLQIRDGLALISRRIESVRKALAALARNHRDTPMA
GRTHLQHALPVTFGYKAAVWLSAFDRHAARLEEISPRVLVVEFSGASGTLASLGTRGLDVQRELARELNLGVPSITWHSA
RDAVAETVQFLALVSGSLGKLAMDISIMMTTELGEVAEPFVRHRGASSTMPQKQNPVSCELILAGARIVRNHATSMLDAM
IHDFERATGPWHLEWSAVPEGFAVASGILYQAEFMLGGLQVFPDRMRENLDHSRGLIVAEAVMMALAPHTGRKEAHDIVY
LGCRRAVEDKTGLFEVLRTMPEVAKPLGEEALRDLTDPRNYLGSAGAMVDNVLGGR
>Mature_456_residues
MRISMATFFPQSVTALDSPLYGRSFADDKMRELFSAQSFISRCVETEVALARAQARLGIIPEDAAAGITAAARTFAPEME
RLRDDTEIVGYPILPLVEQLSAHAGEAGKYLHWGATTQDIMDTATVLQIRDGLALISRRIESVRKALAALARNHRDTPMA
GRTHLQHALPVTFGYKAAVWLSAFDRHAARLEEISPRVLVVEFSGASGTLASLGTRGLDVQRELARELNLGVPSITWHSA
RDAVAETVQFLALVSGSLGKLAMDISIMMTTELGEVAEPFVRHRGASSTMPQKQNPVSCELILAGARIVRNHATSMLDAM
IHDFERATGPWHLEWSAVPEGFAVASGILYQAEFMLGGLQVFPDRMRENLDHSRGLIVAEAVMMALAPHTGRKEAHDIVY
LGCRRAVEDKTGLFEVLRTMPEVAKPLGEEALRDLTDPRNYLGSAGAMVDNVLGGR

Specific function: Catalyzes an anti cycloisomerization [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family [H]

Homologues:

Organism=Homo sapiens, GI31541964, Length=215, Percent_Identity=33.0232558139535, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI68303542, Length=215, Percent_Identity=33.0232558139535, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI68303547, Length=204, Percent_Identity=33.8235294117647, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1790398, Length=313, Percent_Identity=29.073482428115, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17508577, Length=443, Percent_Identity=23.4762979683973, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI32564234, Length=289, Percent_Identity=25.6055363321799, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI32565146, Length=351, Percent_Identity=26.2108262108262, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17553882, Length=357, Percent_Identity=25.4901960784314, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323391, Length=459, Percent_Identity=24.400871459695, Blast_Score=104, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24647570, Length=406, Percent_Identity=27.0935960591133, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI78710009, Length=348, Percent_Identity=25.5747126436782, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI221473854, Length=312, Percent_Identity=28.8461538461538, Blast_Score=66, Evalue=5e-11,
Organism=Drosophila melanogaster, GI78706858, Length=312, Percent_Identity=28.8461538461538, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR012789 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =5.5.1.2 [H]

Molecular weight: Translated: 49748; Mature: 49748

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRISMATFFPQSVTALDSPLYGRSFADDKMRELFSAQSFISRCVETEVALARAQARLGII
CCCCHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PEDAAAGITAAARTFAPEMERLRDDTEIVGYPILPLVEQLSAHAGEAGKYLHWGATTQDI
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCHHHH
MDTATVLQIRDGLALISRRIESVRKALAALARNHRDTPMAGRTHLQHALPVTFGYKAAVW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHH
LSAFDRHAARLEEISPRVLVVEFSGASGTLASLGTRGLDVQRELARELNLGVPSITWHSA
HHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHH
RDAVAETVQFLALVSGSLGKLAMDISIMMTTELGEVAEPFVRHRGASSTMPQKQNPVSCE
HHHHHHHHHHHHHHHCCCHHHHHEEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCEE
LILAGARIVRNHATSMLDAMIHDFERATGPWHLEWSAVPEGFAVASGILYQAEFMLGGLQ
EEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
VFPDRMRENLDHSRGLIVAEAVMMALAPHTGRKEAHDIVYLGCRRAVEDKTGLFEVLRTM
HHHHHHHHCCCHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
PEVAKPLGEEALRDLTDPRNYLGSAGAMVDNVLGGR
HHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHCCC
>Mature Secondary Structure
MRISMATFFPQSVTALDSPLYGRSFADDKMRELFSAQSFISRCVETEVALARAQARLGII
CCCCHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PEDAAAGITAAARTFAPEMERLRDDTEIVGYPILPLVEQLSAHAGEAGKYLHWGATTQDI
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCHHHH
MDTATVLQIRDGLALISRRIESVRKALAALARNHRDTPMAGRTHLQHALPVTFGYKAAVW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHH
LSAFDRHAARLEEISPRVLVVEFSGASGTLASLGTRGLDVQRELARELNLGVPSITWHSA
HHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHH
RDAVAETVQFLALVSGSLGKLAMDISIMMTTELGEVAEPFVRHRGASSTMPQKQNPVSCE
HHHHHHHHHHHHHHHCCCHHHHHEEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCEE
LILAGARIVRNHATSMLDAMIHDFERATGPWHLEWSAVPEGFAVASGILYQAEFMLGGLQ
EEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
VFPDRMRENLDHSRGLIVAEAVMMALAPHTGRKEAHDIVYLGCRRAVEDKTGLFEVLRTM
HHHHHHHHCCCHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
PEVAKPLGEEALRDLTDPRNYLGSAGAMVDNVLGGR
HHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8063101 [H]