Click here to switch to the map view.

The map label for this gene is mdlC [H]

Identifier: 110635253

GI number: 110635253

Start: 3177802

End: 3179532

Strand: Reverse

Name: mdlC [H]

Synonym: Meso_2924

Alternate gene names: 110635253

Gene position: 3179532-3177802 (Counterclockwise)

Preceding gene: 110635254

Following gene: 110635252

Centisome position: 72.06

GC content: 60.14

Gene sequence:

>1731_bases
ATGAACCAAAAAACGCTAAAGAACGTGACGGTGGCCGAGGCTTACCTGCACCTGCTGGCGGATCGCGGCGTAGACTATTT
TTTTGCCAATGCCGGGACAGACTTTGCCCCGCTCATCGAAGCTTTCGCCAAAGGCGAAGTCGAGGGGACGCGCCTTCCGA
AACCCATCACCGCGCCGCATGAGAATGTCGCGGTATCAATGGCCCTCGGTCACGCCCTGGTCAGCGGTAAACCGCAGGCG
GTGATGGTTCATGTGAACGTGGGTACTGCCAACGCATTGTGCGGCCTGCTCAACGCCCAGCGGGTGCGCGCACCCATGCT
GTTCACCTCCGGGCGAACGCCTATCTTGGAGGAGGGGCTCCCCGGCCATCGGTCCAGCTTTATTCACTGGCCCCAGGAGA
TGTACGACCAGGGCGGTATGGTGCGCGAGGCCGTGAAGTGGGACTACGAACTCCGCAATTCGGTGCAGATTGAAACAGTT
CTCGATCGGGCACTATCGATTGCCAACAATGCGCCGTCGGGTCCGGTCTATCTGACGCTGCCGCGCGAAGTCCTTGCCGA
GACGCTGGAGAGCTTTACCTACACGCCGGGCGGGGGCATGCAGAGATGCTCTGCCCCGTCGGCGGCCAGCGCGGGCTCGA
TTCAACAAGCAGTGGAAATGCTGCAGAAAGCTGAGAACCCGTTGATCATTACGTCGACCAGCGGGCGTACGGCGGGGGGC
GTCGCCGCCCTATCTCGGTTGGCTCTCGAACATGCCATTCCGGTTATCTCATTCAACCCGCGCTACATGGAAATCGCGAG
CGCCCACCCCATGAACATGGGCCAGAATATCAATGCCTGGCTTCCAGAGGCCGATGTGGTTCTGGTGCTCGATACGATCG
TGCCTTGGATACCGTCGGAAACCCGGCCGAACGCTGCTGCCAAGATCATACATATTGGCGAGGACCCGCTCTATACTGAG
GTGCCAATCCGCAGTTTCCGCTGTGATCTGGCGATCGCCGCCGACCTTGAGACGGCGCTGGTCCAAATCGCCGATGGACT
GGCTACTTCCCCATCGAACGATCCTGAACTGGTGGACCGCCGTCGTGCAGCACTTGCGCAAAGGCGCCGGAAGCTGGACG
AGACGAGGCTAGCCGCATGGCAGAAGGCGCAGCTTTCCAAGGAAATCACGCCATTGCATATCAGCCGGACCATCGACAAG
GTGAAGGGCGATGACGGCCTCATCTTCCGCGAATCCGGTATTCGCATCGATCACATGAGCTTCGGAAAGCCGGGGACGTA
TTTCTTTTCTGGCGCGGCCGGGGGGCTGGGCTGGAGCCTTGGCGCGGCGCTGGGCGCGAAGCTGGCGGCACCTGACCGGA
CAGTTATCTCCACGGTGGGTGATGGCTCCTATATGTTCGGTACGCCGCTTGCCGCTCACTATGTGGCTGCCGAGCAGAAG
CTGCCGGTGGTGTTTATCGTCTTTAACAATGGCGGCTGGCACGCAGTGCGCAGGGCAACACGCGGCATGTATAGTGACGG
TCATTCCGCCGAAGCCTCAAGATTCGGCCGCGAACCGCTCACCGACTTTAGCGTTGATATCGGTTTTGAAAAAATCGTGG
AAAGCGTCGGCGGTCACGGCGAACGGGTGGAGCATCCGGACGATCTTCAAAAGGCAATGGATCGGGCGATGGACATCACC
CGTACAGAGGGCCGCCAGGTGCTACTCAACGTGGTCTGCACCACATCCTGA

Upstream 100 bases:

>100_bases
GGCCGCCTGCATGGGGCGGACGCCATCGGTGAGTTCCAGCAGACCAAACACATCTACTTACCCTTTGGCCAAAGCTGAAC
GCGGGCCCACGGAGACCATC

Downstream 100 bases:

>100_bases
CGCTGATTTCTCGCACAAGTTTCCTGGCAATGTGCCGATGCCGTCTATGCAGGTGTCGGCGGCATGCAGGCTAGGCAGAC
CTGATATGGCAAATGGCATG

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: BFD; BFDC [H]

Number of amino acids: Translated: 576; Mature: 576

Protein sequence:

>576_residues
MNQKTLKNVTVAEAYLHLLADRGVDYFFANAGTDFAPLIEAFAKGEVEGTRLPKPITAPHENVAVSMALGHALVSGKPQA
VMVHVNVGTANALCGLLNAQRVRAPMLFTSGRTPILEEGLPGHRSSFIHWPQEMYDQGGMVREAVKWDYELRNSVQIETV
LDRALSIANNAPSGPVYLTLPREVLAETLESFTYTPGGGMQRCSAPSAASAGSIQQAVEMLQKAENPLIITSTSGRTAGG
VAALSRLALEHAIPVISFNPRYMEIASAHPMNMGQNINAWLPEADVVLVLDTIVPWIPSETRPNAAAKIIHIGEDPLYTE
VPIRSFRCDLAIAADLETALVQIADGLATSPSNDPELVDRRRAALAQRRRKLDETRLAAWQKAQLSKEITPLHISRTIDK
VKGDDGLIFRESGIRIDHMSFGKPGTYFFSGAAGGLGWSLGAALGAKLAAPDRTVISTVGDGSYMFGTPLAAHYVAAEQK
LPVVFIVFNNGGWHAVRRATRGMYSDGHSAEASRFGREPLTDFSVDIGFEKIVESVGGHGERVEHPDDLQKAMDRAMDIT
RTEGRQVLLNVVCTTS

Sequences:

>Translated_576_residues
MNQKTLKNVTVAEAYLHLLADRGVDYFFANAGTDFAPLIEAFAKGEVEGTRLPKPITAPHENVAVSMALGHALVSGKPQA
VMVHVNVGTANALCGLLNAQRVRAPMLFTSGRTPILEEGLPGHRSSFIHWPQEMYDQGGMVREAVKWDYELRNSVQIETV
LDRALSIANNAPSGPVYLTLPREVLAETLESFTYTPGGGMQRCSAPSAASAGSIQQAVEMLQKAENPLIITSTSGRTAGG
VAALSRLALEHAIPVISFNPRYMEIASAHPMNMGQNINAWLPEADVVLVLDTIVPWIPSETRPNAAAKIIHIGEDPLYTE
VPIRSFRCDLAIAADLETALVQIADGLATSPSNDPELVDRRRAALAQRRRKLDETRLAAWQKAQLSKEITPLHISRTIDK
VKGDDGLIFRESGIRIDHMSFGKPGTYFFSGAAGGLGWSLGAALGAKLAAPDRTVISTVGDGSYMFGTPLAAHYVAAEQK
LPVVFIVFNNGGWHAVRRATRGMYSDGHSAEASRFGREPLTDFSVDIGFEKIVESVGGHGERVEHPDDLQKAMDRAMDIT
RTEGRQVLLNVVCTTS
>Mature_576_residues
MNQKTLKNVTVAEAYLHLLADRGVDYFFANAGTDFAPLIEAFAKGEVEGTRLPKPITAPHENVAVSMALGHALVSGKPQA
VMVHVNVGTANALCGLLNAQRVRAPMLFTSGRTPILEEGLPGHRSSFIHWPQEMYDQGGMVREAVKWDYELRNSVQIETV
LDRALSIANNAPSGPVYLTLPREVLAETLESFTYTPGGGMQRCSAPSAASAGSIQQAVEMLQKAENPLIITSTSGRTAGG
VAALSRLALEHAIPVISFNPRYMEIASAHPMNMGQNINAWLPEADVVLVLDTIVPWIPSETRPNAAAKIIHIGEDPLYTE
VPIRSFRCDLAIAADLETALVQIADGLATSPSNDPELVDRRRAALAQRRRKLDETRLAAWQKAQLSKEITPLHISRTIDK
VKGDDGLIFRESGIRIDHMSFGKPGTYFFSGAAGGLGWSLGAALGAKLAAPDRTVISTVGDGSYMFGTPLAAHYVAAEQK
LPVVFIVFNNGGWHAVRRATRGMYSDGHSAEASRFGREPLTDFSVDIGFEKIVESVGGHGERVEHPDDLQKAMDRAMDIT
RTEGRQVLLNVVCTTS

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=521, Percent_Identity=24.5681381957774, Blast_Score=100, Evalue=5e-21,
Organism=Escherichia coli, GI87081685, Length=593, Percent_Identity=24.4519392917369, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1790104, Length=544, Percent_Identity=25.7352941176471, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1786717, Length=567, Percent_Identity=24.3386243386243, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1788716, Length=560, Percent_Identity=21.9642857142857, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787096, Length=288, Percent_Identity=25.3472222222222, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17542570, Length=603, Percent_Identity=23.5489220563847, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17531299, Length=579, Percent_Identity=24.006908462867, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17531301, Length=579, Percent_Identity=24.006908462867, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323755, Length=598, Percent_Identity=23.5785953177258, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320816, Length=582, Percent_Identity=21.9931271477663, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.7 [H]

Molecular weight: Translated: 62274; Mature: 62274

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQKTLKNVTVAEAYLHLLADRGVDYFFANAGTDFAPLIEAFAKGEVEGTRLPKPITAPH
CCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCC
ENVAVSMALGHALVSGKPQAVMVHVNVGTANALCGLLNAQRVRAPMLFTSGRTPILEEGL
CCEEEEHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCEEEECCCCCHHHCCC
PGHRSSFIHWPQEMYDQGGMVREAVKWDYELRNSVQIETVLDRALSIANNAPSGPVYLTL
CCCCCCCEECCHHHHHCCCHHHHHHHHCHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEE
PREVLAETLESFTYTPGGGMQRCSAPSAASAGSIQQAVEMLQKAENPLIITSTSGRTAGG
CHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHH
VAALSRLALEHAIPVISFNPRYMEIASAHPMNMGQNINAWLPEADVVLVLDTIVPWIPSE
HHHHHHHHHHHCCCEEECCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCC
TRPNAAAKIIHIGEDPLYTEVPIRSFRCDLAIAADLETALVQIADGLATSPSNDPELVDR
CCCCCCEEEEECCCCCCEECCCCHHHEECEEEEHHHHHHHHHHHHCCCCCCCCCHHHHHH
RRAALAQRRRKLDETRLAAWQKAQLSKEITPLHISRTIDKVKGDDGLIFRESGIRIDHMS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCEEEECCCCEEEEEC
FGKPGTYFFSGAAGGLGWSLGAALGAKLAAPDRTVISTVGDGSYMFGTPLAAHYVAAEQK
CCCCCCEEECCCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCEEECCCHHHHHHHHCCC
LPVVFIVFNNGGWHAVRRATRGMYSDGHSAEASRFGREPLTDFSVDIGFEKIVESVGGHG
CCEEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCEECCCHHHHHHHHCCCC
ERVEHPDDLQKAMDRAMDITRTEGRQVLLNVVCTTS
CCCCCHHHHHHHHHHHHCCHHCCCCEEEEEEEEECC
>Mature Secondary Structure
MNQKTLKNVTVAEAYLHLLADRGVDYFFANAGTDFAPLIEAFAKGEVEGTRLPKPITAPH
CCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCC
ENVAVSMALGHALVSGKPQAVMVHVNVGTANALCGLLNAQRVRAPMLFTSGRTPILEEGL
CCEEEEHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCEEEECCCCCHHHCCC
PGHRSSFIHWPQEMYDQGGMVREAVKWDYELRNSVQIETVLDRALSIANNAPSGPVYLTL
CCCCCCCEECCHHHHHCCCHHHHHHHHCHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEE
PREVLAETLESFTYTPGGGMQRCSAPSAASAGSIQQAVEMLQKAENPLIITSTSGRTAGG
CHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHH
VAALSRLALEHAIPVISFNPRYMEIASAHPMNMGQNINAWLPEADVVLVLDTIVPWIPSE
HHHHHHHHHHHCCCEEECCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCC
TRPNAAAKIIHIGEDPLYTEVPIRSFRCDLAIAADLETALVQIADGLATSPSNDPELVDR
CCCCCCEEEEECCCCCCEECCCCHHHEECEEEEHHHHHHHHHHHHCCCCCCCCCHHHHHH
RRAALAQRRRKLDETRLAAWQKAQLSKEITPLHISRTIDKVKGDDGLIFRESGIRIDHMS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCEEEECCCCEEEEEC
FGKPGTYFFSGAAGGLGWSLGAALGAKLAAPDRTVISTVGDGSYMFGTPLAAHYVAAEQK
CCCCCCEEECCCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCEEECCCHHHHHHHHCCC
LPVVFIVFNNGGWHAVRRATRGMYSDGHSAEASRFGREPLTDFSVDIGFEKIVESVGGHG
CCEEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCEECCCHHHHHHHHCCCC
ERVEHPDDLQKAMDRAMDITRTEGRQVLLNVVCTTS
CCCCCHHHHHHHHHHHHCCHHCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2271624; 7663351; 9665697 [H]