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The map label for this gene is rfbE [H]

Identifier: 110635112

GI number: 110635112

Start: 3027040

End: 3028143

Strand: Reverse

Name: rfbE [H]

Synonym: Meso_2779

Alternate gene names: 110635112

Gene position: 3028143-3027040 (Counterclockwise)

Preceding gene: 110635113

Following gene: 110635111

Centisome position: 68.63

GC content: 62.05

Gene sequence:

>1104_bases
ATGAGCGGGACGAATCCGAATGGTCCGGACCCGTCAGGGAGCGGCGCGGGGGAGCCGGTCGTCATCAGCGGTGGCAGCGG
CTTTATCGGCTGCAATCTGGCTGAAAGCTTTCTGCGCGACGGCCATGACGTCGTGGTGCTCGACAATCTGAGCCGGCCCG
GAGTGAGCCAGAACCTGAGTTGGCTGAAAGCCGAATTCGGAGAGCGGGTGCATCCTGTCATCGCCGATATACGAGACCTT
GAGGCAATCCGGCCCGTGATCGCGAAAGCCCGGGCGGTGTTTCATCTCGCCGCGCAGACGGCCGTTACGACAAGCCTGGA
CGATCCGCTCGGGGATTTCGCCGTCAATGCAGAAGGTACCTTGAACGTTCTGGAGGCGGTGCGCGCTTCAGGGCGCAAAA
TCCCGGTCATCTTCGCCAGCACGAACAAGGTCTATGGCGCGCTCGGCGACCTCGAAATGGTCGAGCTTGAGGACAGGTAT
GTCCCTTCTTCGGCCGAGGTCCGCGAGCGAGGCATAAGCGAAGACCGGCGGCTTGATTTCTGCACGCCCTATGGCTGTTC
CAAGGGGGTGGCGGATCAGTATGTGCTCGATTATGCGAAGTCCTTCGGTATTCCGACAGCCGTGCTGCGCATGAGCTGCA
TCTATGGCCCGCATCAGTTCGGCACCGAGGACCAGGGCTGGGTGGCCCATTTCCTTATCCGCGCGTTGCAAGGGCAGCCG
ATTTCAATCTACGGAAACGGCAAGCAGGTGCGCGATGTGCTCCACGTGTCGGATGCGGTCGCTGCATACCGCCGCGTGCT
CGACAACATCGATGCCGTAAGCGGCCGGACATTCAATCTCGGCGGAGGCGTGCGCAATGCGGTGAGCCTTCGCCTTGTGC
TCCAGGAGATCCGCCGCATCACGGGAACGGAACCCGTAGTCGGATGGGGAGACTGGCGAGCGGGCGACCAGTACTATTTC
GTCGCGGACACGACCCGTTTGCAGAGCGAACTCGGCTGGAGCGCCACCATTGGCTGGCGCGAAGGGTTGAAGGATCTTGC
CGATTGGCTGCGCAAAGCACGCCGGCTGGAGCCACCCCATCACCAGGAAAGGCTGAGCGCATGA

Upstream 100 bases:

>100_bases
AAGACACGGTCGCGGAGTTCGCTGAATGGGTGCGGGAGACGGGCGCCGTCGACAACGCCGCACAGATGCGCCGGCAATTG
GAAGAGCGGGGGCTGGTCTC

Downstream 100 bases:

>100_bases
ACATGTTCCGCGTGCCGGCGCAGGAAGGTCGCCCGCTCGATCAACGCCGGGTGCTTATGACCGTGGACGCGGTGGGCGGG
GTGTGGCGCTACGCCATGGA

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: CDP-tyvelose 2-epimerase [H]

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MSGTNPNGPDPSGSGAGEPVVISGGSGFIGCNLAESFLRDGHDVVVLDNLSRPGVSQNLSWLKAEFGERVHPVIADIRDL
EAIRPVIAKARAVFHLAAQTAVTTSLDDPLGDFAVNAEGTLNVLEAVRASGRKIPVIFASTNKVYGALGDLEMVELEDRY
VPSSAEVRERGISEDRRLDFCTPYGCSKGVADQYVLDYAKSFGIPTAVLRMSCIYGPHQFGTEDQGWVAHFLIRALQGQP
ISIYGNGKQVRDVLHVSDAVAAYRRVLDNIDAVSGRTFNLGGGVRNAVSLRLVLQEIRRITGTEPVVGWGDWRAGDQYYF
VADTTRLQSELGWSATIGWREGLKDLADWLRKARRLEPPHHQERLSA

Sequences:

>Translated_367_residues
MSGTNPNGPDPSGSGAGEPVVISGGSGFIGCNLAESFLRDGHDVVVLDNLSRPGVSQNLSWLKAEFGERVHPVIADIRDL
EAIRPVIAKARAVFHLAAQTAVTTSLDDPLGDFAVNAEGTLNVLEAVRASGRKIPVIFASTNKVYGALGDLEMVELEDRY
VPSSAEVRERGISEDRRLDFCTPYGCSKGVADQYVLDYAKSFGIPTAVLRMSCIYGPHQFGTEDQGWVAHFLIRALQGQP
ISIYGNGKQVRDVLHVSDAVAAYRRVLDNIDAVSGRTFNLGGGVRNAVSLRLVLQEIRRITGTEPVVGWGDWRAGDQYYF
VADTTRLQSELGWSATIGWREGLKDLADWLRKARRLEPPHHQERLSA
>Mature_366_residues
SGTNPNGPDPSGSGAGEPVVISGGSGFIGCNLAESFLRDGHDVVVLDNLSRPGVSQNLSWLKAEFGERVHPVIADIRDLE
AIRPVIAKARAVFHLAAQTAVTTSLDDPLGDFAVNAEGTLNVLEAVRASGRKIPVIFASTNKVYGALGDLEMVELEDRYV
PSSAEVRERGISEDRRLDFCTPYGCSKGVADQYVLDYAKSFGIPTAVLRMSCIYGPHQFGTEDQGWVAHFLIRALQGQPI
SIYGNGKQVRDVLHVSDAVAAYRRVLDNIDAVSGRTFNLGGGVRNAVSLRLVLQEIRRITGTEPVVGWGDWRAGDQYYFV
ADTTRLQSELGWSATIGWREGLKDLADWLRKARRLEPPHHQERLSA

Specific function: Catalyzes the isomeration of CDP-paratose to CDP- tyvelose [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=347, Percent_Identity=25.3602305475504, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI42516563, Length=335, Percent_Identity=26.2686567164179, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI56237023, Length=359, Percent_Identity=27.2980501392758, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI56118217, Length=359, Percent_Identity=27.2980501392758, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI189083684, Length=359, Percent_Identity=27.2980501392758, Blast_Score=96, Evalue=4e-20,
Organism=Escherichia coli, GI48994969, Length=352, Percent_Identity=28.125, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1788353, Length=348, Percent_Identity=28.1609195402299, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1786974, Length=349, Percent_Identity=29.2263610315186, Blast_Score=98, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17539532, Length=334, Percent_Identity=25.1497005988024, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71982035, Length=360, Percent_Identity=26.9444444444444, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71982038, Length=362, Percent_Identity=26.7955801104972, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17568069, Length=354, Percent_Identity=24.8587570621469, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI115532424, Length=351, Percent_Identity=27.0655270655271, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319493, Length=353, Percent_Identity=24.3626062322946, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21356223, Length=337, Percent_Identity=27.0029673590504, Blast_Score=104, Evalue=8e-23,
Organism=Drosophila melanogaster, GI19923002, Length=356, Percent_Identity=27.5280898876405, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.10 [H]

Molecular weight: Translated: 39999; Mature: 39867

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00436 PEROXIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGTNPNGPDPSGSGAGEPVVISGGSGFIGCNLAESFLRDGHDVVVLDNLSRPGVSQNLS
CCCCCCCCCCCCCCCCCCCEEEECCCCEEEHHHHHHHHHCCCCEEEEECCCCCCCHHHHH
WLKAEFGERVHPVIADIRDLEAIRPVIAKARAVFHLAAQTAVTTSLDDPLGDFAVNAEGT
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCH
LNVLEAVRASGRKIPVIFASTNKVYGALGDLEMVELEDRYVPSSAEVRERGISEDRRLDF
HHHHHHHHHCCCEEEEEEEECCCHHHCCCCEEEEEECCCCCCCCHHHHHCCCCCCCCCCC
CTPYGCSKGVADQYVLDYAKSFGIPTAVLRMSCIYGPHQFGTEDQGWVAHFLIRALQGQP
CCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHCCCC
ISIYGNGKQVRDVLHVSDAVAAYRRVLDNIDAVSGRTFNLGGGVRNAVSLRLVLQEIRRI
EEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCEEECCCCHHHHHHHHHHHHHHHHH
TGTEPVVGWGDWRAGDQYYFVADTTRLQSELGWSATIGWREGLKDLADWLRKARRLEPPH
CCCCCEECCCCCCCCCEEEEEECHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHCCCCCC
HQERLSA
HHHHCCC
>Mature Secondary Structure 
SGTNPNGPDPSGSGAGEPVVISGGSGFIGCNLAESFLRDGHDVVVLDNLSRPGVSQNLS
CCCCCCCCCCCCCCCCCCEEEECCCCEEEHHHHHHHHHCCCCEEEEECCCCCCCHHHHH
WLKAEFGERVHPVIADIRDLEAIRPVIAKARAVFHLAAQTAVTTSLDDPLGDFAVNAEGT
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCH
LNVLEAVRASGRKIPVIFASTNKVYGALGDLEMVELEDRYVPSSAEVRERGISEDRRLDF
HHHHHHHHHCCCEEEEEEEECCCHHHCCCCEEEEEECCCCCCCCHHHHHCCCCCCCCCCC
CTPYGCSKGVADQYVLDYAKSFGIPTAVLRMSCIYGPHQFGTEDQGWVAHFLIRALQGQP
CCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHCCCC
ISIYGNGKQVRDVLHVSDAVAAYRRVLDNIDAVSGRTFNLGGGVRNAVSLRLVLQEIRRI
EEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCEEECCCCHHHHHHHHHHHHHHHHH
TGTEPVVGWGDWRAGDQYYFVADTTRLQSELGWSATIGWREGLKDLADWLRKARRLEPPH
CCCCCEECCCCCCCCCEEEEEECHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHCCCCCC
HQERLSA
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2793833; 11677608; 12644504; 12642575 [H]