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The map label for this gene is gltD [H]

Identifier: 110635077

GI number: 110635077

Start: 2984664

End: 2986118

Strand: Reverse

Name: gltD [H]

Synonym: Meso_2743

Alternate gene names: 110635077

Gene position: 2986118-2984664 (Counterclockwise)

Preceding gene: 110635078

Following gene: 110635073

Centisome position: 67.67

GC content: 62.34

Gene sequence:

>1455_bases
ATGGGCAAGGTTACAGGCTTTCTCGAGATCGACCGGCAGGAGAACAAGTATCAGCCGGCGTCGGACCGGATACGGCACTT
CCGCGAGTTCACGATTCCGATGTCGGATCAGGAGGTCTCGAAACAGGCCGCGCGGTGCATGGATTGCGGCATTCCCTATT
GCCATGGGCCCACGGGATGCCCGGTGCACAATCAGATCCCGGACTGGAACGATCTCGTCTATCAGGGCGATTGGGAGGCG
GCGATCCGCAATCTCCACTCGACCAACAATTTTCCGGAGTGGACGGGGCGCATCTGCCCGGCGCCGTGCGAGGAAGCCTG
TACGCTCAATCTCGAAGACGTGCCGGTGACGATCAAGACCATCGAACAGGCAATCGGCGATAAGGCCTATGAGCTGGGCT
TTGTCCGGCCGCAGCCGGCAGACAGGAAGACCGGAAAGCGTGTGGCGATCATCGGCTCCGGCCCGGCCGGCATGGCGGCG
GCGCAGCAACTCGGCCGCGCCGGCCACGACGTGCATGTCTATGAGCGCGAGAGCAGGCCGGGCGGGCTGATGCGCTACGG
CATCCCCGATTTCAAGATCGAGAAGCGTTATGTCGATCGCCGGGTCGAGCAGATGCAGGGCGAGGGCGTTACCTTCTTTT
GCGGTGTGAATGTCGGTGTCGACAAGCCCGTTTCCGAGCTTCTCGCCGAATATGACGCGGTGCTTTATTGCGGCGGCTCG
GAAAAGCCGAGGCCAGCGGGCATTCCGGGCGTCGAGCTCAATGGCGTCTACGATGCCATGCCCTATCTCGTGCAGCAGAA
CCGGAGAATCGGCGGCGAGAACACCCAATCCGTCGCGTGGGCGTCGGTACCGATCCTGGCCAGCGGCAAGCATGTCGTCG
TTGTGGGCGGCGGTGATACAGCGTCGGATTGTGTGGGCACGGCCTTCCGGCAAGGCGCGGTGCGCGTTACCCAGCTCGAC
ATTCGCCCGAAGCCGCCCGAGAAGGAAGACAAGCTCACCGTCTGGCCCTATTGGGCCACAAAGATGCGAACCTCATCCAG
CCAGGCAGAGGGGGCGGAACGGGAATTCCAGGTGGCGACGCTGGAATTCATAGGTGAAGACGGACAGCTTACCGGCGTGA
AGTGCTGCCAGGTGGACGAGAGGCGCAAGCCCATCGCCGGGACGGAGTTTATTATAAAGGCAGACCTCGCCTTCATCGCG
ATCGGATTTGCCGGGCCGCTGGAAACCGGCATTTTGGACGAACTGGGCGAGAAACTGACGACCGACGTCGATCGGCGCAG
TTCCACCAATGTCGTGGCCAATGACCGGGATTACCGCACTAGCTTGGACAAGCTTTTTGCCGCCGGAGACGTTCGGCGCG
GGCAATCCCTCGTCGTGTGGGCAATTCGCGAGGGCAGGCAGGCGGCGCGCGCGATCGATGAATTCCTCATGGGAAGCTCG
GTCCTGCCGCGATAG

Upstream 100 bases:

>100_bases
GTAGCAAGCAATCGTTGAACGGAGGCGCCCGCGCACGCGGATGCCTCCCTGCGGATCACTTTGGCTGTGGGCGTAAAGCC
TTCAGGAACAAGGAACGAGC

Downstream 100 bases:

>100_bases
CGGATCGGCGCAAGGGTAATATTGTCCTTGCGCCAGGCCTTTCGATCAAGCCTGGCGTTCCCACCAGTCAAGCACGCGCA
TTGCGCGGAGCGTCACCCAT

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MGKVTGFLEIDRQENKYQPASDRIRHFREFTIPMSDQEVSKQAARCMDCGIPYCHGPTGCPVHNQIPDWNDLVYQGDWEA
AIRNLHSTNNFPEWTGRICPAPCEEACTLNLEDVPVTIKTIEQAIGDKAYELGFVRPQPADRKTGKRVAIIGSGPAGMAA
AQQLGRAGHDVHVYERESRPGGLMRYGIPDFKIEKRYVDRRVEQMQGEGVTFFCGVNVGVDKPVSELLAEYDAVLYCGGS
EKPRPAGIPGVELNGVYDAMPYLVQQNRRIGGENTQSVAWASVPILASGKHVVVVGGGDTASDCVGTAFRQGAVRVTQLD
IRPKPPEKEDKLTVWPYWATKMRTSSSQAEGAEREFQVATLEFIGEDGQLTGVKCCQVDERRKPIAGTEFIIKADLAFIA
IGFAGPLETGILDELGEKLTTDVDRRSSTNVVANDRDYRTSLDKLFAAGDVRRGQSLVVWAIREGRQAARAIDEFLMGSS
VLPR

Sequences:

>Translated_484_residues
MGKVTGFLEIDRQENKYQPASDRIRHFREFTIPMSDQEVSKQAARCMDCGIPYCHGPTGCPVHNQIPDWNDLVYQGDWEA
AIRNLHSTNNFPEWTGRICPAPCEEACTLNLEDVPVTIKTIEQAIGDKAYELGFVRPQPADRKTGKRVAIIGSGPAGMAA
AQQLGRAGHDVHVYERESRPGGLMRYGIPDFKIEKRYVDRRVEQMQGEGVTFFCGVNVGVDKPVSELLAEYDAVLYCGGS
EKPRPAGIPGVELNGVYDAMPYLVQQNRRIGGENTQSVAWASVPILASGKHVVVVGGGDTASDCVGTAFRQGAVRVTQLD
IRPKPPEKEDKLTVWPYWATKMRTSSSQAEGAEREFQVATLEFIGEDGQLTGVKCCQVDERRKPIAGTEFIIKADLAFIA
IGFAGPLETGILDELGEKLTTDVDRRSSTNVVANDRDYRTSLDKLFAAGDVRRGQSLVVWAIREGRQAARAIDEFLMGSS
VLPR
>Mature_483_residues
GKVTGFLEIDRQENKYQPASDRIRHFREFTIPMSDQEVSKQAARCMDCGIPYCHGPTGCPVHNQIPDWNDLVYQGDWEAA
IRNLHSTNNFPEWTGRICPAPCEEACTLNLEDVPVTIKTIEQAIGDKAYELGFVRPQPADRKTGKRVAIIGSGPAGMAAA
QQLGRAGHDVHVYERESRPGGLMRYGIPDFKIEKRYVDRRVEQMQGEGVTFFCGVNVGVDKPVSELLAEYDAVLYCGGSE
KPRPAGIPGVELNGVYDAMPYLVQQNRRIGGENTQSVAWASVPILASGKHVVVVGGGDTASDCVGTAFRQGAVRVTQLDI
RPKPPEKEDKLTVWPYWATKMRTSSSQAEGAEREFQVATLEFIGEDGQLTGVKCCQVDERRKPIAGTEFIIKADLAFIAI
GFAGPLETGILDELGEKLTTDVDRRSSTNVVANDRDYRTSLDKLFAAGDVRRGQSLVVWAIREGRQAARAIDEFLMGSSV
LPR

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=363, Percent_Identity=26.1707988980716, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI111118983, Length=227, Percent_Identity=29.9559471365639, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI111118981, Length=226, Percent_Identity=28.7610619469027, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1789606, Length=490, Percent_Identity=37.1428571428571, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI1788811, Length=472, Percent_Identity=37.5, Blast_Score=259, Evalue=4e-70,
Organism=Escherichia coli, GI87082180, Length=472, Percent_Identity=34.3220338983051, Blast_Score=214, Evalue=8e-57,
Organism=Escherichia coli, GI1788468, Length=456, Percent_Identity=27.1929824561404, Blast_Score=145, Evalue=7e-36,
Organism=Escherichia coli, GI1789243, Length=433, Percent_Identity=28.8683602771363, Blast_Score=119, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17570289, Length=502, Percent_Identity=42.0318725099602, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71984108, Length=307, Percent_Identity=28.3387622149837, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17543860, Length=179, Percent_Identity=35.7541899441341, Blast_Score=88, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6320030, Length=501, Percent_Identity=40.1197604790419, Blast_Score=376, Evalue=1e-105,
Organism=Drosophila melanogaster, GI28574881, Length=495, Percent_Identity=45.4545454545455, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24665539, Length=495, Percent_Identity=45.4545454545455, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24665547, Length=495, Percent_Identity=45.4545454545455, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24665543, Length=495, Percent_Identity=45.4545454545455, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24640763, Length=475, Percent_Identity=24.6315789473684, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI18858217, Length=475, Percent_Identity=24.6315789473684, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI17137178, Length=162, Percent_Identity=30.8641975308642, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 53252; Mature: 53121

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKVTGFLEIDRQENKYQPASDRIRHFREFTIPMSDQEVSKQAARCMDCGIPYCHGPTGC
CCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
PVHNQIPDWNDLVYQGDWEAAIRNLHSTNNFPEWTGRICPAPCEEACTLNLEDVPVTIKT
CCCCCCCCCCCEEECCCHHHHHHHHHCCCCCCHHCCCCCCCCCCHHHEECCCCCCCHHHH
IEQAIGDKAYELGFVRPQPADRKTGKRVAIIGSGPAGMAAAQQLGRAGHDVHVYERESRP
HHHHHCCCCEEECCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCC
GGLMRYGIPDFKIEKRYVDRRVEQMQGEGVTFFCGVNVGVDKPVSELLAEYDAVLYCGGS
CCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHCCEEEEECCC
EKPRPAGIPGVELNGVYDAMPYLVQQNRRIGGENTQSVAWASVPILASGKHVVVVGGGDT
CCCCCCCCCCEEECCHHHHHHHHHHHCCCCCCCCCCCEEEEECEEEECCCEEEEEECCCC
ASDCVGTAFRQGAVRVTQLDIRPKPPEKEDKLTVWPYWATKMRTSSSQAEGAEREFQVAT
HHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEEEEHHHHCCCCCCCCCCCCHHEEEEE
LEFIGEDGQLTGVKCCQVDERRKPIAGTEFIIKADLAFIAIGFAGPLETGILDELGEKLT
EEEECCCCCCCCEEEECCCCCCCCCCCCEEEEEECEEEEEEECCCCCHHHHHHHHHHHHH
TDVDRRSSTNVVANDRDYRTSLDKLFAAGDVRRGQSLVVWAIREGRQAARAIDEFLMGSS
CCHHCCCCCCEEECCCHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCC
VLPR
CCCC
>Mature Secondary Structure 
GKVTGFLEIDRQENKYQPASDRIRHFREFTIPMSDQEVSKQAARCMDCGIPYCHGPTGC
CCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
PVHNQIPDWNDLVYQGDWEAAIRNLHSTNNFPEWTGRICPAPCEEACTLNLEDVPVTIKT
CCCCCCCCCCCEEECCCHHHHHHHHHCCCCCCHHCCCCCCCCCCHHHEECCCCCCCHHHH
IEQAIGDKAYELGFVRPQPADRKTGKRVAIIGSGPAGMAAAQQLGRAGHDVHVYERESRP
HHHHHCCCCEEECCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCC
GGLMRYGIPDFKIEKRYVDRRVEQMQGEGVTFFCGVNVGVDKPVSELLAEYDAVLYCGGS
CCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHCCEEEEECCC
EKPRPAGIPGVELNGVYDAMPYLVQQNRRIGGENTQSVAWASVPILASGKHVVVVGGGDT
CCCCCCCCCCEEECCHHHHHHHHHHHCCCCCCCCCCCEEEEECEEEECCCEEEEEECCCC
ASDCVGTAFRQGAVRVTQLDIRPKPPEKEDKLTVWPYWATKMRTSSSQAEGAEREFQVAT
HHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEEEEHHHHCCCCCCCCCCCCHHEEEEE
LEFIGEDGQLTGVKCCQVDERRKPIAGTEFIIKADLAFIAIGFAGPLETGILDELGEKLT
EEEECCCCCCCCEEEECCCCCCCCCCCCEEEEEECEEEEEEECCCCCHHHHHHHHHHHHH
TDVDRRSSTNVVANDRDYRTSLDKLFAAGDVRRGQSLVVWAIREGRQAARAIDEFLMGSS
CCHHCCCCCCEEECCCHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCC
VLPR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]