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The map label for this gene is gatA [H]

Identifier: 110635048

GI number: 110635048

Start: 2951455

End: 2952642

Strand: Reverse

Name: gatA [H]

Synonym: Meso_2714

Alternate gene names: 110635048

Gene position: 2952642-2951455 (Counterclockwise)

Preceding gene: 110635051

Following gene: 110635047

Centisome position: 66.92

GC content: 63.55

Gene sequence:

>1188_bases
ATGACGGGCACGCGGGACAGCCTGAACGCGTTTCTGGACTACCCGCAAACAGCGGTGCCCTCATCGCCCACCGGCCTGCT
TCGTGGCCTCTCGTTCGGCGTGAAGGATATTTACGACGTTGCCGGCTATCCGACCGGATGCGGGAACCCGCAGAAGGAAG
CCGAACGAGGCCCTGTATCCCACTCTTCGCCGGCGATCCAGGCCTTGCTCGACGCAGGCGCGGCCTTTGCGGGCAAGACA
CAGACCGACGAACTCGCCTTCTCGATGATGGGCATGAACGCGCATTTTCCCCCGCCGGTGAATCCGAGGGCGCCGGAGCG
CGTCACGGGAGGCTCATCCTCCGGGTCGGCGGCGGCCGTGGCTGGCGGACTGGCAGACTTCGCCATCGGATCGGACACGA
ACGGATCGGTGCGGATTCCCGCTTCCTTCTGCGGCCTGATAGGCTTGCGCACCACGCACGGGCGAATCAGCATGGAAGGC
GCCATGCCGCTCGCGCCGTCATTCGATACATTTGGCTGGTTTGCTCGCGATGCCCGCACATACGAGGCTGTCGGCGAGGT
GCTCCTGGGAGAGGACTTTCATGGCGGGACACTGCGCTCGCCGGTTCGAATCAGTGAATTCGAGGCACGGCTTTTCGGCC
AAGCGGAGAGCGCGGCCTATGGAGAGATGCTCGGCCGTGTGCTCGATCACTTCGGGAAATCTGCGGGATATGTCGCCCTT
CCCGGCGAAATCGAGGATCTGTATGAGTGCTTCCGGCAGATCCAGGCCCATGAGGCTTGGCTTTCCCAGGCATATTTTCT
GTCGCAGCAGGACCGGGAACTCGGCCCGGGAGTGAAAGAGCGCTTCGCCTACGGCCGGTCGATCAGTGATGAGACGGCAG
AAAACGCAAGGCGAAGGCGGCGCGAATTCCAGGCCGATTTCAATGCACTGATGCGCCCTGATATGGTGGTGGTAATGCCA
ACACAGCCCTCGGCTGCGCCGCTCAAGATCGCCACTCTTGAGGAACAGGAAAGCTATCGCCACAGGGGCCTTGCGCTCAC
CTGCATCGCCGGGCTGCTCGGCTGGCCGCAGATCTCCATACCGCTCGGAGAGGTGCATGGCGCGCCCTTCGGCATTTCCC
TCCTCGGGCCGGCAGGCAGCGACCGCCAGCTCATACGGCTCGCCTCCAAGATCCTCGCGGGTTCGTAG

Upstream 100 bases:

>100_bases
TATAGGCGCGTTCGATGTCACAATCAACGCCAAATTTTCGGGCGCTTGAAAAATTTGGCACATGCGCCGATTGTCTGCCG
ATCAGCCAAGGAGAATCGTC

Downstream 100 bases:

>100_bases
GATCATGGATACGTTGACGCGGATGCGCGCTTTCATCCAAGTCGCCGATGCGGAAGGATTTTCAGCGGCGGCGCGGAAAT
CGGGCCGCTCAAAGGCTTTG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MTGTRDSLNAFLDYPQTAVPSSPTGLLRGLSFGVKDIYDVAGYPTGCGNPQKEAERGPVSHSSPAIQALLDAGAAFAGKT
QTDELAFSMMGMNAHFPPPVNPRAPERVTGGSSSGSAAAVAGGLADFAIGSDTNGSVRIPASFCGLIGLRTTHGRISMEG
AMPLAPSFDTFGWFARDARTYEAVGEVLLGEDFHGGTLRSPVRISEFEARLFGQAESAAYGEMLGRVLDHFGKSAGYVAL
PGEIEDLYECFRQIQAHEAWLSQAYFLSQQDRELGPGVKERFAYGRSISDETAENARRRRREFQADFNALMRPDMVVVMP
TQPSAAPLKIATLEEQESYRHRGLALTCIAGLLGWPQISIPLGEVHGAPFGISLLGPAGSDRQLIRLASKILAGS

Sequences:

>Translated_395_residues
MTGTRDSLNAFLDYPQTAVPSSPTGLLRGLSFGVKDIYDVAGYPTGCGNPQKEAERGPVSHSSPAIQALLDAGAAFAGKT
QTDELAFSMMGMNAHFPPPVNPRAPERVTGGSSSGSAAAVAGGLADFAIGSDTNGSVRIPASFCGLIGLRTTHGRISMEG
AMPLAPSFDTFGWFARDARTYEAVGEVLLGEDFHGGTLRSPVRISEFEARLFGQAESAAYGEMLGRVLDHFGKSAGYVAL
PGEIEDLYECFRQIQAHEAWLSQAYFLSQQDRELGPGVKERFAYGRSISDETAENARRRRREFQADFNALMRPDMVVVMP
TQPSAAPLKIATLEEQESYRHRGLALTCIAGLLGWPQISIPLGEVHGAPFGISLLGPAGSDRQLIRLASKILAGS
>Mature_394_residues
TGTRDSLNAFLDYPQTAVPSSPTGLLRGLSFGVKDIYDVAGYPTGCGNPQKEAERGPVSHSSPAIQALLDAGAAFAGKTQ
TDELAFSMMGMNAHFPPPVNPRAPERVTGGSSSGSAAAVAGGLADFAIGSDTNGSVRIPASFCGLIGLRTTHGRISMEGA
MPLAPSFDTFGWFARDARTYEAVGEVLLGEDFHGGTLRSPVRISEFEARLFGQAESAAYGEMLGRVLDHFGKSAGYVALP
GEIEDLYECFRQIQAHEAWLSQAYFLSQQDRELGPGVKERFAYGRSISDETAENARRRRREFQADFNALMRPDMVVVMPT
QPSAAPLKIATLEEQESYRHRGLALTCIAGLLGWPQISIPLGEVHGAPFGISLLGPAGSDRQLIRLASKILAGS

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=429, Percent_Identity=27.2727272727273, Blast_Score=83, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17543272, Length=407, Percent_Identity=28.009828009828, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17556264, Length=173, Percent_Identity=31.2138728323699, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17537465, Length=180, Percent_Identity=29.4444444444444, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=427, Percent_Identity=25.2927400468384, Blast_Score=90, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6319685, Length=382, Percent_Identity=26.4397905759162, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24648113, Length=431, Percent_Identity=28.0742459396752, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24644968, Length=437, Percent_Identity=20.3661327231121, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21356731, Length=403, Percent_Identity=23.5732009925558, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 42157; Mature: 42026

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGTRDSLNAFLDYPQTAVPSSPTGLLRGLSFGVKDIYDVAGYPTGCGNPQKEAERGPVS
CCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCC
HSSPAIQALLDAGAAFAGKTQTDELAFSMMGMNAHFPPPVNPRAPERVTGGSSSGSAAAV
CCCHHHHHHHHHCCHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
AGGLADFAIGSDTNGSVRIPASFCGLIGLRTTHGRISMEGAMPLAPSFDTFGWFARDART
HCCHHHHCCCCCCCCCEECCHHHHHHHHEECCCCEEEECCCCCCCCCCCHHHHHHHHHHH
YEAVGEVLLGEDFHGGTLRSPVRISEFEARLFGQAESAAYGEMLGRVLDHFGKSAGYVAL
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEC
PGEIEDLYECFRQIQAHEAWLSQAYFLSQQDRELGPGVKERFAYGRSISDETAENARRRR
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHCCCCCHHHHHHHHHHH
REFQADFNALMRPDMVVVMPTQPSAAPLKIATLEEQESYRHRGLALTCIAGLLGWPQISI
HHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCHHHHHHHCCCHHHHHHHHHCCCCEEC
PLGEVHGAPFGISLLGPAGSDRQLIRLASKILAGS
CHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TGTRDSLNAFLDYPQTAVPSSPTGLLRGLSFGVKDIYDVAGYPTGCGNPQKEAERGPVS
CCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCC
HSSPAIQALLDAGAAFAGKTQTDELAFSMMGMNAHFPPPVNPRAPERVTGGSSSGSAAAV
CCCHHHHHHHHHCCHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
AGGLADFAIGSDTNGSVRIPASFCGLIGLRTTHGRISMEGAMPLAPSFDTFGWFARDART
HCCHHHHCCCCCCCCCEECCHHHHHHHHEECCCCEEEECCCCCCCCCCCHHHHHHHHHHH
YEAVGEVLLGEDFHGGTLRSPVRISEFEARLFGQAESAAYGEMLGRVLDHFGKSAGYVAL
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEC
PGEIEDLYECFRQIQAHEAWLSQAYFLSQQDRELGPGVKERFAYGRSISDETAENARRRR
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHCCCCCHHHHHHHHHHH
REFQADFNALMRPDMVVVMPTQPSAAPLKIATLEEQESYRHRGLALTCIAGLLGWPQISI
HHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCHHHHHHHCCCHHHHHHHHHCCCCEEC
PLGEVHGAPFGISLLGPAGSDRQLIRLASKILAGS
CHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]