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The map label for this gene is gcvA [H]

Identifier: 110634728

GI number: 110634728

Start: 2540908

End: 2541798

Strand: Direct

Name: gcvA [H]

Synonym: Meso_2386

Alternate gene names: 110634728

Gene position: 2540908-2541798 (Clockwise)

Preceding gene: 110634726

Following gene: 110634733

Centisome position: 57.59

GC content: 56.9

Gene sequence:

>891_bases
GTGCCAAGCGAAACGCAGCTTCCGCCGCTGACCACACTTCGGATTTTTGAGGCTGCGGCGAGACACCAGAGCTTCCTGCT
GGCCGCGGGAGAACTGAACATCACCCCGGGTGCCGTAAGCAAGCAGGTGAAGCAGCTCGAGCTTTCCCTCGGGCAGCCTC
TTTTCGAACGGAACCACAGACAGGTGCGGCTCACCAAAGCCGGGCAGATCTTTTTCAAGGGGATTAGCCAAGCATTCGAA
GACATTCGCGCAGTGACAAAGGCAGTTGCCATTAGGCCGGACGGTGAGCGCCTGGTTCTATGGTGCGCGCCTTGGTTTCT
TAGAGCGTGGCTGCTCCCCCGGCTCGAACGCTTTCGATCAAGCTCGCCGAAGATACAATTGGACGTGCTGACCGGAGATT
CGAGCGACGCCATTTCTCCGCGCGCAGATATCGCGATACGGCTCGGTTCGGGTGACTGGCCACGAGTGAAATCGGAAATG
CTGATTTCCCAAGAGATAACCGCGGTCTGCACCCCACGATATCTGAAGCGTGAAAAAATTGGTGCTCCCTACAGCTTTTC
GGATTTTGTCGTTCTGGACAGCTCCAACACTCCCGACCATCTCCATCTCTGGAAGGAGGCGGCCGGCTTTCAGCAAGTCG
ACCCAAAAGAGCGAATTGTTTTTTTGAGCAGCGAATCCGCCTTTGCGGCGGCGCAGAGCGACCACGGAATCGCACTCGTC
CACCCACGTTTCATCGAGAAAGAGTTGGCCACGGGAGATTTAGTGCGCCCTTGGGGCATCACCACCAGAACGAATAAGCA
CTACTACCTGACCCGCAACGAGAATTTCAGGTTGCCGCCCGCGGCCGCAGCGTTCCGCAAGTGGTTGCTCGATGAGTTTA
AACGGACTTAG

Upstream 100 bases:

>100_bases
TCCACGGTTTGTCGCCTCGAAATAACAACAGGAGGGTTTGGATTGACAAACGACAAATGCTCCGCTTCAAGTGAACTGAA
TTCATCTAAGGCCCTTTGCT

Downstream 100 bases:

>100_bases
CCTGGCACAGATTCAAGCCGTTCGAGAGATCTAGGCTTTCCAGCTGGAGATCCCCTCCCTCGAAGAATAAGCCGCGCCTT
CTTCGGCGCGGCTGAGGTCA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MPSETQLPPLTTLRIFEAAARHQSFLLAAGELNITPGAVSKQVKQLELSLGQPLFERNHRQVRLTKAGQIFFKGISQAFE
DIRAVTKAVAIRPDGERLVLWCAPWFLRAWLLPRLERFRSSSPKIQLDVLTGDSSDAISPRADIAIRLGSGDWPRVKSEM
LISQEITAVCTPRYLKREKIGAPYSFSDFVVLDSSNTPDHLHLWKEAAGFQQVDPKERIVFLSSESAFAAAQSDHGIALV
HPRFIEKELATGDLVRPWGITTRTNKHYYLTRNENFRLPPAAAAFRKWLLDEFKRT

Sequences:

>Translated_296_residues
MPSETQLPPLTTLRIFEAAARHQSFLLAAGELNITPGAVSKQVKQLELSLGQPLFERNHRQVRLTKAGQIFFKGISQAFE
DIRAVTKAVAIRPDGERLVLWCAPWFLRAWLLPRLERFRSSSPKIQLDVLTGDSSDAISPRADIAIRLGSGDWPRVKSEM
LISQEITAVCTPRYLKREKIGAPYSFSDFVVLDSSNTPDHLHLWKEAAGFQQVDPKERIVFLSSESAFAAAQSDHGIALV
HPRFIEKELATGDLVRPWGITTRTNKHYYLTRNENFRLPPAAAAFRKWLLDEFKRT
>Mature_295_residues
PSETQLPPLTTLRIFEAAARHQSFLLAAGELNITPGAVSKQVKQLELSLGQPLFERNHRQVRLTKAGQIFFKGISQAFED
IRAVTKAVAIRPDGERLVLWCAPWFLRAWLLPRLERFRSSSPKIQLDVLTGDSSDAISPRADIAIRLGSGDWPRVKSEML
ISQEITAVCTPRYLKREKIGAPYSFSDFVVLDSSNTPDHLHLWKEAAGFQQVDPKERIVFLSSESAFAAAQSDHGIALVH
PRFIEKELATGDLVRPWGITTRTNKHYYLTRNENFRLPPAAAAFRKWLLDEFKRT

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=287, Percent_Identity=32.7526132404181, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1788706, Length=296, Percent_Identity=31.7567567567568, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1786448, Length=297, Percent_Identity=31.986531986532, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1786401, Length=252, Percent_Identity=28.1746031746032, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790208, Length=144, Percent_Identity=31.9444444444444, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI157672245, Length=208, Percent_Identity=27.4038461538462, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33473; Mature: 33342

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSETQLPPLTTLRIFEAAARHQSFLLAAGELNITPGAVSKQVKQLELSLGQPLFERNHR
CCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHCCCC
QVRLTKAGQIFFKGISQAFEDIRAVTKAVAIRPDGERLVLWCAPWFLRAWLLPRLERFRS
EEEEEHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECHHHHHHHHHHHHHHHCC
SSPKIQLDVLTGDSSDAISPRADIAIRLGSGDWPRVKSEMLISQEITAVCTPRYLKREKI
CCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHC
GAPYSFSDFVVLDSSNTPDHLHLWKEAAGFQQVDPKERIVFLSSESAFAAAQSDHGIALV
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEECCCCCCEEEE
HPRFIEKELATGDLVRPWGITTRTNKHYYLTRNENFRLPPAAAAFRKWLLDEFKRT
CHHHHHHHHCCCCCCCCCCCEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PSETQLPPLTTLRIFEAAARHQSFLLAAGELNITPGAVSKQVKQLELSLGQPLFERNHR
CCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHCCCC
QVRLTKAGQIFFKGISQAFEDIRAVTKAVAIRPDGERLVLWCAPWFLRAWLLPRLERFRS
EEEEEHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECHHHHHHHHHHHHHHHCC
SSPKIQLDVLTGDSSDAISPRADIAIRLGSGDWPRVKSEMLISQEITAVCTPRYLKREKI
CCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHC
GAPYSFSDFVVLDSSNTPDHLHLWKEAAGFQQVDPKERIVFLSSESAFAAAQSDHGIALV
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEECCCCCCEEEE
HPRFIEKELATGDLVRPWGITTRTNKHYYLTRNENFRLPPAAAAFRKWLLDEFKRT
CHHHHHHHHCCCCCCCCCCCEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]