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The map label for this gene is potI [H]

Identifier: 110634713

GI number: 110634713

Start: 2523240

End: 2524064

Strand: Reverse

Name: potI [H]

Synonym: Meso_2370

Alternate gene names: 110634713

Gene position: 2524064-2523240 (Counterclockwise)

Preceding gene: 110634714

Following gene: 110634712

Centisome position: 57.2

GC content: 60.61

Gene sequence:

>825_bases
ATGGATAAAGAACGCGACGTTGAGGCGCTGGGCGGCTCGGCGCCATCCCCCCTGGTCGCCACCGCCGCTTGGCTGGGGTT
CGTCTACCTTCTCCTCCCAAGCCTCATCATCATCCCGATGTCTTTCGGAGACACGCGCGAGATCGTTTTCCCGCCCGCCA
CTTATACGCTCGACCTTTACCGGGAATACTTCACCAACCCGACCTGGACCGGTTCCACCATCCAGAGCATCCGCATCGCG
GTAATCGCGACACTCGCCGGTTTGGCACTAGGCACATCCGCCGCCTATGGCATTGTGCGGGGCAGCTTCCGTGGGAAGGT
CCTGCTTGGCGCGCTCCTGCTGAGCCCGTTTTTCGTGCCGGCGGTGGTCAAGGCCCTTGGCTTCTACATCTATTTCGCGG
CGCTCGGCATTCAGGGAACGACTCTTAGCGTGGTGCTGGGGCACACGCTGTTCGTCACACCTTACGTCATGATCGTGATG
ATGGCCGCGTTGCGGAACGTGGACGCGCAGATCGAGGCAGCGGGAATGATTATGGGCGCAAGCCGCATTTACGTGTTTCG
TAAGGTGACGCTTCCGATGCTCCGCAATGCGCTGATCGCGGCAGGGCTGTTCGCCTTCCTCATGTCCTTCGACGAGATGA
TCATCGCATTCTTCCTGTCGGGCTTCGGAACGGCGACCCTGCCCGTCAAGATGTACGAAAGCATCGTGCACGAGGTGTCG
CCGGTACTGTCGGCTATATCGGCGCTGCTGACATTTCTTGCCCTTGTCATCTGCTTGGCCGTCACCGCGCTGAGCGGGAG
AAGCGGCAGACAACCGTCGCACTGA

Upstream 100 bases:

>100_bases
GCTGCGGCCGTTTCCATCGTTCTGCTGCTCCTGTCATGTCTGATTTTGCTTGCCCTCACCCGGGTGAGGGGGGCTCAACT
GTTTGGGGAGCAGCCGCGCC

Downstream 100 bases:

>100_bases
GTGTATGGAGCGGGCTCGGCTGGTCCGCCAGCAGAATGGTGCTCCTCAGAAAATCGGGTTCGCATCGGTGCGTAAAGCAT
TAATCGTAGGATACCAAGCA

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MDKERDVEALGGSAPSPLVATAAWLGFVYLLLPSLIIIPMSFGDTREIVFPPATYTLDLYREYFTNPTWTGSTIQSIRIA
VIATLAGLALGTSAAYGIVRGSFRGKVLLGALLLSPFFVPAVVKALGFYIYFAALGIQGTTLSVVLGHTLFVTPYVMIVM
MAALRNVDAQIEAAGMIMGASRIYVFRKVTLPMLRNALIAAGLFAFLMSFDEMIIAFFLSGFGTATLPVKMYESIVHEVS
PVLSAISALLTFLALVICLAVTALSGRSGRQPSH

Sequences:

>Translated_274_residues
MDKERDVEALGGSAPSPLVATAAWLGFVYLLLPSLIIIPMSFGDTREIVFPPATYTLDLYREYFTNPTWTGSTIQSIRIA
VIATLAGLALGTSAAYGIVRGSFRGKVLLGALLLSPFFVPAVVKALGFYIYFAALGIQGTTLSVVLGHTLFVTPYVMIVM
MAALRNVDAQIEAAGMIMGASRIYVFRKVTLPMLRNALIAAGLFAFLMSFDEMIIAFFLSGFGTATLPVKMYESIVHEVS
PVLSAISALLTFLALVICLAVTALSGRSGRQPSH
>Mature_274_residues
MDKERDVEALGGSAPSPLVATAAWLGFVYLLLPSLIIIPMSFGDTREIVFPPATYTLDLYREYFTNPTWTGSTIQSIRIA
VIATLAGLALGTSAAYGIVRGSFRGKVLLGALLLSPFFVPAVVKALGFYIYFAALGIQGTTLSVVLGHTLFVTPYVMIVM
MAALRNVDAQIEAAGMIMGASRIYVFRKVTLPMLRNALIAAGLFAFLMSFDEMIIAFFLSGFGTATLPVKMYESIVHEVS
PVLSAISALLTFLALVICLAVTALSGRSGRQPSH

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=233, Percent_Identity=33.9055793991416, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1787368, Length=206, Percent_Identity=27.6699029126214, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787714, Length=233, Percent_Identity=32.618025751073, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787369, Length=207, Percent_Identity=25.1207729468599, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29359; Mature: 29359

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]