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The map label for this gene is dat [H]

Identifier: 110634702

GI number: 110634702

Start: 2511032

End: 2511889

Strand: Reverse

Name: dat [H]

Synonym: Meso_2359

Alternate gene names: 110634702

Gene position: 2511889-2511032 (Counterclockwise)

Preceding gene: 110634703

Following gene: 110634700

Centisome position: 56.93

GC content: 57.93

Gene sequence:

>858_bases
GTGCCTGACGATTCACGCGTGGTTTATGTCAATGGCGATTACGTCGCCGCCGCAGAAGCGAAAGTCTCCGTATTCGACCG
CGGATTCTTGTTCGGCGATGGGATCTACGAGGTTACGGCCGTCGTTGATGGCCAGCTTGTGGACAGCGACGCCCATCTGA
GCCGGCTGGCCCGATCCGCACGGGAAATCGGCATCTCGCTTCCCTGGACGGCAGCCGAGATCGAATCCGTCGAAAAACAG
TTGATCGTGAAGAACGGCCTGCGCGAGGGCCTCGTCTATCTTCAGGTAACGCGCGGCATCGCCGACCGGGACTTCAACTA
TGGCTCGCTCGTGCCGAGCATTGTGCTGTTCACTCAGAAGAAATTACTTATCGAATCGCAAGCGGCCGCGCAGGGTATTG
CCGTCAAATCTGTTCGCGATCTTCGATGGGCGAGGCGCGATATAAAGAGCGTGTGCCTTCTCCCGCAGGTTCTGGCAAAG
GCCCTTGCCCGGTCCGAGGGATGTGCCGAGGCCTGGATGATCGACGAGAATGGACTTGTAACGGAAGGTGGGTCGTCGAC
GGCGTATATCGTGAAGAATCGCAGGATCTTTACGCGAGAGAATTCGCAAGCAATCCTGCCCGGCTGTACCCGTGCGTCGC
TCCTTCATCTGAGGCGACATCACGCCGTTGAGGTCATTGAACGTTCTTTTTCGCTCGAAGAGGCTTATGCCGCGGATGAA
GCGTTCATAACCAGTGCCTCGTCTTTCGTAACACCTGTCATATCAATCGACGACCGCCGGATCGGCGAAGGTAAGCCCGG
CGCGGTCACAAAGGCACTGCGGGAAGCTTACATCCGGTTCGCGCGTCGGGCCACATAG

Upstream 100 bases:

>100_bases
GATTTCATCGTGGCCGAGTTCAGCAAGCTTGTCGAGCACGTGGCCGGCCTGCCGGCGGAGCAGCTGGTCGAACCGAAGCC
GTCGCGGGTGGGAGGCACGG

Downstream 100 bases:

>100_bases
GCAGTAGGACAGAAGGTACACGGCCGCCATCGCCCCAGAGTCCCAGCATCAGCTGCCGACGCCGCTTCGCTGTGCCGAGA
GTTGAGTCAGAGTTCGTGTT

Product: branched chain amino acid: 2-keto-4-methylthiobutyrate aminotransferase

Products: NA

Alternate protein names: D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MPDDSRVVYVNGDYVAAAEAKVSVFDRGFLFGDGIYEVTAVVDGQLVDSDAHLSRLARSAREIGISLPWTAAEIESVEKQ
LIVKNGLREGLVYLQVTRGIADRDFNYGSLVPSIVLFTQKKLLIESQAAAQGIAVKSVRDLRWARRDIKSVCLLPQVLAK
ALARSEGCAEAWMIDENGLVTEGGSSTAYIVKNRRIFTRENSQAILPGCTRASLLHLRRHHAVEVIERSFSLEEAYAADE
AFITSASSFVTPVISIDDRRIGEGKPGAVTKALREAYIRFARRAT

Sequences:

>Translated_285_residues
MPDDSRVVYVNGDYVAAAEAKVSVFDRGFLFGDGIYEVTAVVDGQLVDSDAHLSRLARSAREIGISLPWTAAEIESVEKQ
LIVKNGLREGLVYLQVTRGIADRDFNYGSLVPSIVLFTQKKLLIESQAAAQGIAVKSVRDLRWARRDIKSVCLLPQVLAK
ALARSEGCAEAWMIDENGLVTEGGSSTAYIVKNRRIFTRENSQAILPGCTRASLLHLRRHHAVEVIERSFSLEEAYAADE
AFITSASSFVTPVISIDDRRIGEGKPGAVTKALREAYIRFARRAT
>Mature_284_residues
PDDSRVVYVNGDYVAAAEAKVSVFDRGFLFGDGIYEVTAVVDGQLVDSDAHLSRLARSAREIGISLPWTAAEIESVEKQL
IVKNGLREGLVYLQVTRGIADRDFNYGSLVPSIVLFTQKKLLIESQAAAQGIAVKSVRDLRWARRDIKSVCLLPQVLAKA
LARSEGCAEAWMIDENGLVTEGGSSTAYIVKNRRIFTRENSQAILPGCTRASLLHLRRHHAVEVIERSFSLEEAYAADEA
FITSASSFVTPVISIDDRRIGEGKPGAVTKALREAYIRFARRAT

Specific function: Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha- keto acid in

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=282, Percent_Identity=26.5957446808511, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787338, Length=246, Percent_Identity=25.609756097561, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005784 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.21 [H]

Molecular weight: Translated: 31315; Mature: 31184

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDDSRVVYVNGDYVAAAEAKVSVFDRGFLFGDGIYEVTAVVDGQLVDSDAHLSRLARSA
CCCCCEEEEECCCEEEECCCEEEEEECCEEECCCCEEEEEEECCEEECCHHHHHHHHHHH
REIGISLPWTAAEIESVEKQLIVKNGLREGLVYLQVTRGIADRDFNYGSLVPSIVLFTQK
HHCCCCCCCCHHHHHHHHHHHHHHHCHHHCEEEEEEECCCCCCCCCCHHHHHHHHHHHHH
KLLIESQAAAQGIAVKSVRDLRWARRDIKSVCLLPQVLAKALARSEGCAEAWMIDENGLV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEE
TEGGSSTAYIVKNRRIFTRENSQAILPGCTRASLLHLRRHHAVEVIERSFSLEEAYAADE
ECCCCCEEEEEECCEEEEECCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCH
AFITSASSFVTPVISIDDRRIGEGKPGAVTKALREAYIRFARRAT
HHHHCCHHHHCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PDDSRVVYVNGDYVAAAEAKVSVFDRGFLFGDGIYEVTAVVDGQLVDSDAHLSRLARSA
CCCCEEEEECCCEEEECCCEEEEEECCEEECCCCEEEEEEECCEEECCHHHHHHHHHHH
REIGISLPWTAAEIESVEKQLIVKNGLREGLVYLQVTRGIADRDFNYGSLVPSIVLFTQK
HHCCCCCCCCHHHHHHHHHHHHHHHCHHHCEEEEEEECCCCCCCCCCHHHHHHHHHHHHH
KLLIESQAAAQGIAVKSVRDLRWARRDIKSVCLLPQVLAKALARSEGCAEAWMIDENGLV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEE
TEGGSSTAYIVKNRRIFTRENSQAILPGCTRASLLHLRRHHAVEVIERSFSLEEAYAADE
ECCCCCEEEEEECCEEEEECCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCH
AFITSASSFVTPVISIDDRRIGEGKPGAVTKALREAYIRFARRAT
HHHHCCHHHHCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9003455 [H]