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The map label for this gene is fixI [H]

Identifier: 110634602

GI number: 110634602

Start: 2408793

End: 2411033

Strand: Direct

Name: fixI [H]

Synonym: Meso_2255

Alternate gene names: 110634602

Gene position: 2408793-2411033 (Clockwise)

Preceding gene: 110634601

Following gene: 110634603

Centisome position: 54.59

GC content: 69.57

Gene sequence:

>2241_bases
GTGGCCGGCTGCTGCGCCCCTGGAGCCGACATCGCGCCAGTGGGCCAACCCGCCGACGAGGAGGTGCGCCTTGCGAGCCG
GGCCCTCTCCGAAACGCTGATGCAGACGGATTTCAGCGTGCCGGCCGCCCATTGCGGCGCCTGCATCAAGACGATCGAGA
AAGCGCTCCTGGCATTGCCTGGCGTGGAGGCGGCAAGGCTCAACCTATCGACCCGGCGTGTTTCGGTGCAGTGGCGGAAG
AACGGTCCCGTGCCACCCTTCTTCGACGCCTTGGCGGGAGCCGGTCACGACGCCACGCTCTATACGCATGACCAGTCGGA
CGACGATCCCGAGTTCGGCCGGCTTCTGCGCGCCACCGCCGTGGCAGGCTTCGCCGCCATGAACATCATGCTGCTCTCGG
TCTCCGTATGGTCCGGCGCCGATGCCGAGACGCGTCATGCCTTCCACATGGTCTCGGCCCTACTGGCCCTGCCGGCCATC
GCCTATTCGGGCCGCGTCTTCTTCGCCTCCGCGTGGTCGGCGCTGAAAGCCCGCACGACCAACATGGACGTGCCGATCTC
CGTCGGCATCCTGCTGACGCTCGCGCTTAGCCTGCACGACACGTTCCGGAACGAGCCGCATGCCTATTTCGACGCCGTGG
TTTCGCTCACTTTCTTCCTTCTGATCGGCCGCACGCTCGACCACATGATGCGCCGCAAGGCCAGGACCGCCATCTCCGGT
CTGGCGCGCATCATGCCGCGCGGCGCGGTGACGGTCTCCTCGGATGGCCTGAGCAGCTACAAGGACGTGCGCGAGATCGC
ACCGGGCGAGACGCTGCTCGTGACACCAGGCGAGCGCATCCCGCTCGACGGCATCGCCATGCACGGCGGCGGCGAGCTGG
ATGCTCAGCTTGTCACGGGCGAGGCCACGCCGATCATCGTCACGCACGGCGACAAGGTCAGCGCCGGCATGCTGAATCTC
ACGGGTGCACTGACCATGCGCGCCGAGCGAAAAGCAGAGGATTCTTTCCTCGCCGAGATGATGCGCATGATGGAGGCAGC
CGAGGCCGACTGTTCGCGCTACCGGCGGATCGCCGACCGCGCTGCAAGCCTCTATTCGCCGGTCATCCACATTCTGGCCC
TCGCCTCCTTCATCGGCTGGATGGCGGCGACCGGCGACTGGCACCGCGCGCTCACCATTGCCGTCTCGGTGCTGATCATC
ACCTGCCCCTGTGCCCTCGGTCTCGCCGTGCCGATGGTGCAGGTGATGGCCGCGCGCCGGCTCTTCGAGCACGGCATCGC
CCTCAAGGAAGGAAGCGCGCTGGAGCGGCTGGCCGGGATCGACACAGCCGTTTTCGACAAGACCGGAACACTGACCCTGG
GCGACCTGCGCGTCGCCGCCTCTACCGTGCCAGAGCGCGACCTCACAGCCGCCGCGGCCCTTGCGGCCCGCTCCCGCCAC
CCCGCCGCGCGCGCCATCGCACGGCTTGATCCGGCCGGCGAAGACCTGCCGGTGGAAGACTTCGCCGAGCAGCCGGGCCT
CGGGATCGAGGGACGGATCGACGGCGCGTGCTATCGCCTCGGCCGACCCTCCTGGGCGGGCGCGGAGGCAAGCCCGGCAA
GCGGCCGCAGCATGAGCGCGCTGTCGCGCGATGGTGCTCCCCTCGGGACGTTCGCCTTCGCCGACACGCCGCGCGCCGAT
GCCAAAGGCGCCGTCCGCGCCCTCCTGGAGCGCGGCATCGCCGTCGAGATCGTGTCGGGCGACGGCGCACAGGCAGTCGC
GGCCGCCGCGCATCATCTCGGCATCGACACCTACCGGGCCGCGATGCTGCCGGCGGACAAGGTGGCGCGGCTGGAAGAGT
TGCGCCGCCAGGGCCACACCGTGCTGATGGTCGGCGACGGGCTGAACGACGCTCCCGCCCTTGCCGCCGCGCACGTCTCC
ATGGCCCCGGGCAGCGCAGCGGAAGTGGGGCGCAACGCGGCCGACATGGTCTTCTTCAGCGGTGCCTTGAGCGCCGTGCC
GGCGGCGCTTGCGACCGCCCAACGCGCGGCGACGCTGGTGCGCCAGAACCTGGCGCTTGCGGTCGCCTACAATGTCGCCG
TCCTGCCGCTGGCGCTGGCCGGCCACGTCACCCCGCTGATCGCCGCCATCGCCATGTCTTCCTCCTCAATCCTCGTCGTC
GCCAATGCCCTCAGGCTGCGCGGGGCGGCGCAGACGTCGGCCGCGTCGCCTACGCCGAAACTGGTCGGGGCGTTGTCATG
A

Upstream 100 bases:

>100_bases
CTCGCCGTCGCCACCGGGCTTCTCGGCCTCGTCATCATCTTTCCGCTTCTCGGCCATGCCACCTGGCACGCCTATGAAGC
CATAGCCCGGAGCTGAGACG

Downstream 100 bases:

>100_bases
ACTATCTCGTCTGGCTCGTCCCGCTCGCCATTCTCATGGGCATAGCCGCCCTCGCCACCTTCCTCTGGTCGTTACGCGAC
GGGCAGTATGAAGACCTGGA

Product: heavy metal translocating P-type ATPase

Products: ADP; Orthophosphate. [C]

Alternate protein names: E1-E2 type cation ATPase FixI [H]

Number of amino acids: Translated: 746; Mature: 745

Protein sequence:

>746_residues
MAGCCAPGADIAPVGQPADEEVRLASRALSETLMQTDFSVPAAHCGACIKTIEKALLALPGVEAARLNLSTRRVSVQWRK
NGPVPPFFDALAGAGHDATLYTHDQSDDDPEFGRLLRATAVAGFAAMNIMLLSVSVWSGADAETRHAFHMVSALLALPAI
AYSGRVFFASAWSALKARTTNMDVPISVGILLTLALSLHDTFRNEPHAYFDAVVSLTFFLLIGRTLDHMMRRKARTAISG
LARIMPRGAVTVSSDGLSSYKDVREIAPGETLLVTPGERIPLDGIAMHGGGELDAQLVTGEATPIIVTHGDKVSAGMLNL
TGALTMRAERKAEDSFLAEMMRMMEAAEADCSRYRRIADRAASLYSPVIHILALASFIGWMAATGDWHRALTIAVSVLII
TCPCALGLAVPMVQVMAARRLFEHGIALKEGSALERLAGIDTAVFDKTGTLTLGDLRVAASTVPERDLTAAAALAARSRH
PAARAIARLDPAGEDLPVEDFAEQPGLGIEGRIDGACYRLGRPSWAGAEASPASGRSMSALSRDGAPLGTFAFADTPRAD
AKGAVRALLERGIAVEIVSGDGAQAVAAAAHHLGIDTYRAAMLPADKVARLEELRRQGHTVLMVGDGLNDAPALAAAHVS
MAPGSAAEVGRNAADMVFFSGALSAVPAALATAQRAATLVRQNLALAVAYNVAVLPLALAGHVTPLIAAIAMSSSSILVV
ANALRLRGAAQTSAASPTPKLVGALS

Sequences:

>Translated_746_residues
MAGCCAPGADIAPVGQPADEEVRLASRALSETLMQTDFSVPAAHCGACIKTIEKALLALPGVEAARLNLSTRRVSVQWRK
NGPVPPFFDALAGAGHDATLYTHDQSDDDPEFGRLLRATAVAGFAAMNIMLLSVSVWSGADAETRHAFHMVSALLALPAI
AYSGRVFFASAWSALKARTTNMDVPISVGILLTLALSLHDTFRNEPHAYFDAVVSLTFFLLIGRTLDHMMRRKARTAISG
LARIMPRGAVTVSSDGLSSYKDVREIAPGETLLVTPGERIPLDGIAMHGGGELDAQLVTGEATPIIVTHGDKVSAGMLNL
TGALTMRAERKAEDSFLAEMMRMMEAAEADCSRYRRIADRAASLYSPVIHILALASFIGWMAATGDWHRALTIAVSVLII
TCPCALGLAVPMVQVMAARRLFEHGIALKEGSALERLAGIDTAVFDKTGTLTLGDLRVAASTVPERDLTAAAALAARSRH
PAARAIARLDPAGEDLPVEDFAEQPGLGIEGRIDGACYRLGRPSWAGAEASPASGRSMSALSRDGAPLGTFAFADTPRAD
AKGAVRALLERGIAVEIVSGDGAQAVAAAAHHLGIDTYRAAMLPADKVARLEELRRQGHTVLMVGDGLNDAPALAAAHVS
MAPGSAAEVGRNAADMVFFSGALSAVPAALATAQRAATLVRQNLALAVAYNVAVLPLALAGHVTPLIAAIAMSSSSILVV
ANALRLRGAAQTSAASPTPKLVGALS
>Mature_745_residues
AGCCAPGADIAPVGQPADEEVRLASRALSETLMQTDFSVPAAHCGACIKTIEKALLALPGVEAARLNLSTRRVSVQWRKN
GPVPPFFDALAGAGHDATLYTHDQSDDDPEFGRLLRATAVAGFAAMNIMLLSVSVWSGADAETRHAFHMVSALLALPAIA
YSGRVFFASAWSALKARTTNMDVPISVGILLTLALSLHDTFRNEPHAYFDAVVSLTFFLLIGRTLDHMMRRKARTAISGL
ARIMPRGAVTVSSDGLSSYKDVREIAPGETLLVTPGERIPLDGIAMHGGGELDAQLVTGEATPIIVTHGDKVSAGMLNLT
GALTMRAERKAEDSFLAEMMRMMEAAEADCSRYRRIADRAASLYSPVIHILALASFIGWMAATGDWHRALTIAVSVLIIT
CPCALGLAVPMVQVMAARRLFEHGIALKEGSALERLAGIDTAVFDKTGTLTLGDLRVAASTVPERDLTAAAALAARSRHP
AARAIARLDPAGEDLPVEDFAEQPGLGIEGRIDGACYRLGRPSWAGAEASPASGRSMSALSRDGAPLGTFAFADTPRADA
KGAVRALLERGIAVEIVSGDGAQAVAAAAHHLGIDTYRAAMLPADKVARLEELRRQGHTVLMVGDGLNDAPALAAAHVSM
APGSAAEVGRNAADMVFFSGALSAVPAALATAQRAATLVRQNLALAVAYNVAVLPLALAGHVTPLIAAIAMSSSSILVVA
NALRLRGAAQTSAASPTPKLVGALS

Specific function: FixI is a pump of a specific cation involved in symbiotic nitrogen fixation. The four proteins FixG, FixH, FixI, and FixS may participate in a membrane-bound complex coupling the FixI cation pump with a redox process catalyzed by FixG [H]

COG id: COG2217

COG function: function code P; Cation transport ATPase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI55743071, Length=784, Percent_Identity=26.6581632653061, Blast_Score=244, Evalue=2e-64,
Organism=Homo sapiens, GI55743073, Length=500, Percent_Identity=30.6, Blast_Score=192, Evalue=1e-48,
Organism=Homo sapiens, GI115529486, Length=564, Percent_Identity=24.822695035461, Blast_Score=169, Evalue=8e-42,
Organism=Homo sapiens, GI48762687, Length=268, Percent_Identity=25.7462686567164, Blast_Score=77, Evalue=7e-14,
Organism=Homo sapiens, GI48762691, Length=269, Percent_Identity=25.6505576208178, Blast_Score=77, Evalue=8e-14,
Organism=Homo sapiens, GI48762685, Length=268, Percent_Identity=25.7462686567164, Blast_Score=77, Evalue=9e-14,
Organism=Homo sapiens, GI48762689, Length=269, Percent_Identity=25.6505576208178, Blast_Score=76, Evalue=9e-14,
Organism=Escherichia coli, GI1786691, Length=731, Percent_Identity=31.8741450068399, Blast_Score=265, Evalue=9e-72,
Organism=Escherichia coli, GI1789879, Length=720, Percent_Identity=29.0277777777778, Blast_Score=208, Evalue=1e-54,
Organism=Escherichia coli, GI1786914, Length=428, Percent_Identity=27.803738317757, Blast_Score=124, Evalue=3e-29,
Organism=Escherichia coli, GI2367363, Length=152, Percent_Identity=29.6052631578947, Blast_Score=74, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17556548, Length=521, Percent_Identity=26.8714011516315, Blast_Score=153, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI71997262, Length=598, Percent_Identity=22.2408026755853, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319772, Length=562, Percent_Identity=28.1138790035587, Blast_Score=204, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6320475, Length=619, Percent_Identity=26.4943457189015, Blast_Score=203, Evalue=7e-53,
Organism=Drosophila melanogaster, GI221329854, Length=514, Percent_Identity=28.2101167315175, Blast_Score=163, Evalue=4e-40,
Organism=Drosophila melanogaster, GI281366617, Length=168, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24668708, Length=168, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24668704, Length=168, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24668696, Length=168, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=4e-11,
Organism=Drosophila melanogaster, GI161085803, Length=168, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008250
- InterPro:   IPR006403
- InterPro:   IPR000695
- InterPro:   IPR006416
- InterPro:   IPR001757
- InterPro:   IPR018303
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00122 E1-E2_ATPase; PF00702 Hydrolase [H]

EC number: 3.6.3.4 [C]

Molecular weight: Translated: 77780; Mature: 77649

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00154 ATPASE_E1_E2 ; PS50846 HMA_2 ; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGCCAPGADIAPVGQPADEEVRLASRALSETLMQTDFSVPAAHCGACIKTIEKALLALP
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
GVEAARLNLSTRRVSVQWRKNGPVPPFFDALAGAGHDATLYTHDQSDDDPEFGRLLRATA
CCCHHEEEEEEEEEEEEECCCCCCCHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHH
VAGFAAMNIMLLSVSVWSGADAETRHAFHMVSALLALPAIAYSGRVFFASAWSALKARTT
HHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHCC
NMDVPISVGILLTLALSLHDTFRNEPHAYFDAVVSLTFFLLIGRTLDHMMRRKARTAISG
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LARIMPRGAVTVSSDGLSSYKDVREIAPGETLLVTPGERIPLDGIAMHGGGELDAQLVTG
HHHHCCCCCEEEECCCHHHHHHHHHCCCCCEEEECCCCCCCCCCEEECCCCCCCEEEEEC
EATPIIVTHGDKVSAGMLNLTGALTMRAERKAEDSFLAEMMRMMEAAEADCSRYRRIADR
CCCEEEEECCCCCCCCHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AASLYSPVIHILALASFIGWMAATGDWHRALTIAVSVLIITCPCALGLAVPMVQVMAARR
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFEHGIALKEGSALERLAGIDTAVFDKTGTLTLGDLRVAASTVPERDLTAAAALAARSRH
HHHCCCCCCCCHHHHHHHCCCHHHCCCCCCEEECCHHHHHHCCCCHHHHHHHHHHHHCCC
PAARAIARLDPAGEDLPVEDFAEQPGLGIEGRIDGACYRLGRPSWAGAEASPASGRSMSA
HHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCHHHEECCCCCCCCCCCCCCCCCCHHH
LSRDGAPLGTFAFADTPRADAKGAVRALLERGIAVEIVSGDGAQAVAAAAHHLGIDTYRA
HHCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCHHHHH
AMLPADKVARLEELRRQGHTVLMVGDGLNDAPALAAAHVSMAPGSAAEVGRNAADMVFFS
HHCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHCCCCCCHHHHCCCHHHHHHHH
GALSAVPAALATAQRAATLVRQNLALAVAYNVAVLPLALAGHVTPLIAAIAMSSSSILVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
ANALRLRGAAQTSAASPTPKLVGALS
EHHHHHCCCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure 
AGCCAPGADIAPVGQPADEEVRLASRALSETLMQTDFSVPAAHCGACIKTIEKALLALP
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
GVEAARLNLSTRRVSVQWRKNGPVPPFFDALAGAGHDATLYTHDQSDDDPEFGRLLRATA
CCCHHEEEEEEEEEEEEECCCCCCCHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHH
VAGFAAMNIMLLSVSVWSGADAETRHAFHMVSALLALPAIAYSGRVFFASAWSALKARTT
HHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHCC
NMDVPISVGILLTLALSLHDTFRNEPHAYFDAVVSLTFFLLIGRTLDHMMRRKARTAISG
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LARIMPRGAVTVSSDGLSSYKDVREIAPGETLLVTPGERIPLDGIAMHGGGELDAQLVTG
HHHHCCCCCEEEECCCHHHHHHHHHCCCCCEEEECCCCCCCCCCEEECCCCCCCEEEEEC
EATPIIVTHGDKVSAGMLNLTGALTMRAERKAEDSFLAEMMRMMEAAEADCSRYRRIADR
CCCEEEEECCCCCCCCHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AASLYSPVIHILALASFIGWMAATGDWHRALTIAVSVLIITCPCALGLAVPMVQVMAARR
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFEHGIALKEGSALERLAGIDTAVFDKTGTLTLGDLRVAASTVPERDLTAAAALAARSRH
HHHCCCCCCCCHHHHHHHCCCHHHCCCCCCEEECCHHHHHHCCCCHHHHHHHHHHHHCCC
PAARAIARLDPAGEDLPVEDFAEQPGLGIEGRIDGACYRLGRPSWAGAEASPASGRSMSA
HHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCHHHEECCCCCCCCCCCCCCCCCCHHH
LSRDGAPLGTFAFADTPRADAKGAVRALLERGIAVEIVSGDGAQAVAAAAHHLGIDTYRA
HHCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCHHHHH
AMLPADKVARLEELRRQGHTVLMVGDGLNDAPALAAAHVSMAPGSAAEVGRNAADMVFFS
HHCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHCCCCCCHHHHCCCHHHHHHHH
GALSAVPAALATAQRAATLVRQNLALAVAYNVAVLPLALAGHVTPLIAAIAMSSSSILVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
ANALRLRGAAQTSAASPTPKLVGALS
EHHHHHCCCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Cu [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; H2O [C]

Specific reaction: ATP + H2O = ADP + Orthophosphate. [C]

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2536685; 11481432 [H]