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The map label for this gene is yeiT [H]

Identifier: 110634407

GI number: 110634407

Start: 2206950

End: 2208302

Strand: Reverse

Name: yeiT [H]

Synonym: Meso_2057

Alternate gene names: 110634407

Gene position: 2208302-2206950 (Counterclockwise)

Preceding gene: 110634409

Following gene: 110634406

Centisome position: 50.05

GC content: 62.31

Gene sequence:

>1353_bases
ATGGCTCAGCGCCATTATGAGGACGGCATCGTCTCGGGCCGCTTCTCGCCGGAAGACTATGCGCAGAATTTCTCCGATCT
TCATCCCGCCCTCGACACGCATGAAGCTCGGGTGGAGGCGGATCGCTGTTACTTCTGCTACGACGCGCCCTGCATGCGGG
CCTGTCCGACAACGATCGACATTCCACTGTTCATCAGGCAGATCTCTACGGGCAATCCTCTTGGCGCGGCAAGGACAATT
TTCGATCAGAACATCTTTGGCGGCATGTGCGCCCGTGTCTGTCCCACCGAGACCTTGTGCGAGGAAGCCTGCGTGCGCGA
GCATGCGGAGGGAAAGCCCGTGCAGATCGGGCGTCTGCAGCGATTTGCGACGGACGCGGCAATGGCGCGCGGCAAGCGAT
TTTATACGCGGGCGGCGGCGAGCGGCAAAAAGGTGGCGGTTGTCGGCGCCGGGCCAGCAGGGCTTGCCTGCGCACACCGT
CTTGCCATGTATGGCCACGAGGTGACGGTATACGAAGCGCGGCCCAAGCCCGGCGGGCTCAACGAATACGGCATTGCCGC
CTACAAAACGCCCGGCGGCTTCGCGCAGGCCGAGGTGGACTACATCCTTTCCATCGGCGGCATCACGATCGAGCACGGCA
AAGCGCTCGGTCGCGACTTTACCCTTGCCGGCCTTGCGGCGGGCTCTGATGCGGTGTTCCTGGCCATGGGGCTTGCGGGC
GTGAACGCCCTGCGTTGCGAGGGCGAGGAAGCGGATGGCGTGGAAAACGCCGTCGACTACATCGCAACGCTTCGGCAGGC
AGACGATCTCTCGAAACTTCCGATCGGCGGGCGCGTCGTCGTCATCGGCGGCGGCATGACGGCAATCGATGCCGCCATTC
AGTCCAGGCTCTTAGGCGCCGAGGAAGTGACTATCTGCTATCGCCGCGGCAAGGAGCATATGAACGCTTCCGAATGGGAG
CAGGAGCTTGCCGCTGCCAAGGGAGTCACCATCCGGCATTGGATGCAGCCACGCCGGGTGATCGCGGAGGCGAACCGCGT
CAGCGGCATAGAAATGGAATATACCGCCATTGTCGACGGCAGGCTGATCGGCACCGGCGACACGCTCATCCTCAAAGCCG
ACCAGATCTTCAAGGCCATCGGGCAAAGCTTCGATCCGGCGGTGCTGAATGGCGGTGGCGATGCGATTGTATTCGAGAAG
GACCGTATCCGGGTGGATGGTGAAGGCCGAACCTCCATGCCGAAAGTCTGGGCCGGTGGAGACTGCATTCTGGGCGGTGA
CGATCTGACTGTTTCAGCGGTCGCCCAAGGCCGGGATGCAGCGGAAAGCATTCATCGAAGTCTGATCGCATGA

Upstream 100 bases:

>100_bases
CCAGCTATTCTTTTGCGGGCGCGGCGTTTACATGGAATTTGACTGATTGATAAAAAAACAATCTGTGTTACCGTTCTCAA
AAAGAAGAAAGGGGAACGGT

Downstream 100 bases:

>100_bases
ATCCGAGGGTGCCATGTTTGTCAAATTCGCTGAACTGCCCGTTTTCAATCAGGATCGCGCCGTTTCTTTCTACAGCGAAA
ATCTCGGCTGCACGGTCGCG

Product: putative oxidoreductase

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MAQRHYEDGIVSGRFSPEDYAQNFSDLHPALDTHEARVEADRCYFCYDAPCMRACPTTIDIPLFIRQISTGNPLGAARTI
FDQNIFGGMCARVCPTETLCEEACVREHAEGKPVQIGRLQRFATDAAMARGKRFYTRAAASGKKVAVVGAGPAGLACAHR
LAMYGHEVTVYEARPKPGGLNEYGIAAYKTPGGFAQAEVDYILSIGGITIEHGKALGRDFTLAGLAAGSDAVFLAMGLAG
VNALRCEGEEADGVENAVDYIATLRQADDLSKLPIGGRVVVIGGGMTAIDAAIQSRLLGAEEVTICYRRGKEHMNASEWE
QELAAAKGVTIRHWMQPRRVIAEANRVSGIEMEYTAIVDGRLIGTGDTLILKADQIFKAIGQSFDPAVLNGGGDAIVFEK
DRIRVDGEGRTSMPKVWAGGDCILGGDDLTVSAVAQGRDAAESIHRSLIA

Sequences:

>Translated_450_residues
MAQRHYEDGIVSGRFSPEDYAQNFSDLHPALDTHEARVEADRCYFCYDAPCMRACPTTIDIPLFIRQISTGNPLGAARTI
FDQNIFGGMCARVCPTETLCEEACVREHAEGKPVQIGRLQRFATDAAMARGKRFYTRAAASGKKVAVVGAGPAGLACAHR
LAMYGHEVTVYEARPKPGGLNEYGIAAYKTPGGFAQAEVDYILSIGGITIEHGKALGRDFTLAGLAAGSDAVFLAMGLAG
VNALRCEGEEADGVENAVDYIATLRQADDLSKLPIGGRVVVIGGGMTAIDAAIQSRLLGAEEVTICYRRGKEHMNASEWE
QELAAAKGVTIRHWMQPRRVIAEANRVSGIEMEYTAIVDGRLIGTGDTLILKADQIFKAIGQSFDPAVLNGGGDAIVFEK
DRIRVDGEGRTSMPKVWAGGDCILGGDDLTVSAVAQGRDAAESIHRSLIA
>Mature_449_residues
AQRHYEDGIVSGRFSPEDYAQNFSDLHPALDTHEARVEADRCYFCYDAPCMRACPTTIDIPLFIRQISTGNPLGAARTIF
DQNIFGGMCARVCPTETLCEEACVREHAEGKPVQIGRLQRFATDAAMARGKRFYTRAAASGKKVAVVGAGPAGLACAHRL
AMYGHEVTVYEARPKPGGLNEYGIAAYKTPGGFAQAEVDYILSIGGITIEHGKALGRDFTLAGLAAGSDAVFLAMGLAGV
NALRCEGEEADGVENAVDYIATLRQADDLSKLPIGGRVVVIGGGMTAIDAAIQSRLLGAEEVTICYRRGKEHMNASEWEQ
ELAAAKGVTIRHWMQPRRVIAEANRVSGIEMEYTAIVDGRLIGTGDTLILKADQIFKAIGQSFDPAVLNGGGDAIVFEKD
RIRVDGEGRTSMPKVWAGGDCILGGDDLTVSAVAQGRDAAESIHRSLIA

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli glutamate synthase [NADPH] small chain (GltD) and to E.coli AegA [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=472, Percent_Identity=31.5677966101695, Blast_Score=189, Evalue=5e-48,
Organism=Homo sapiens, GI237757300, Length=127, Percent_Identity=40.1574803149606, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1788468, Length=430, Percent_Identity=38.1395348837209, Blast_Score=225, Evalue=5e-60,
Organism=Escherichia coli, GI1789606, Length=454, Percent_Identity=34.1409691629956, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI87082180, Length=455, Percent_Identity=33.8461538461538, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1788811, Length=446, Percent_Identity=33.6322869955157, Blast_Score=181, Evalue=8e-47,
Organism=Escherichia coli, GI1789243, Length=406, Percent_Identity=30.0492610837438, Blast_Score=130, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71984108, Length=452, Percent_Identity=32.7433628318584, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17570289, Length=459, Percent_Identity=26.5795206971678, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320030, Length=475, Percent_Identity=26.9473684210526, Blast_Score=107, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24640763, Length=452, Percent_Identity=33.8495575221239, Blast_Score=203, Evalue=2e-52,
Organism=Drosophila melanogaster, GI18858217, Length=452, Percent_Identity=33.8495575221239, Blast_Score=203, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24665547, Length=465, Percent_Identity=27.3118279569892, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24665543, Length=465, Percent_Identity=27.3118279569892, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI28574881, Length=465, Percent_Identity=27.3118279569892, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24665539, Length=465, Percent_Identity=27.3118279569892, Blast_Score=108, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 48070; Mature: 47939

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQRHYEDGIVSGRFSPEDYAQNFSDLHPALDTHEARVEADRCYFCYDAPCMRACPTTID
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHCCCCCEEEEECCCHHHHCCCCCC
IPLFIRQISTGNPLGAARTIFDQNIFGGMCARVCPTETLCEEACVREHAEGKPVQIGRLQ
HHHHEEECCCCCCCCHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEHHHHH
RFATDAAMARGKRFYTRAAASGKKVAVVGAGPAGLACAHRLAMYGHEVTVYEARPKPGGL
HHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCEEEEEECCCCCCCC
NEYGIAAYKTPGGFAQAEVDYILSIGGITIEHGKALGRDFTLAGLAAGSDAVFLAMGLAG
CCCCEEEEECCCCCCHHCEEEEEEECCEEEECCCCCCCCEEEEEECCCCCCEEEEHHHHC
VNALRCEGEEADGVENAVDYIATLRQADDLSKLPIGGRVVVIGGGMTAIDAAIQSRLLGA
CCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCC
EEVTICYRRGKEHMNASEWEQELAAAKGVTIRHWMQPRRVIAEANRVSGIEMEYTAIVDG
HHHHHHHHCCHHHCCHHHHHHHHHHHCCCCHHHHHCHHHHHHHHHHCCCCEEEEEEEECC
RLIGTGDTLILKADQIFKAIGQSFDPAVLNGGGDAIVFEKDRIRVDGEGRTSMPKVWAGG
EEEECCCEEEEEHHHHHHHHCCCCCCEEEECCCCEEEEECCEEEECCCCCCCCCEEEECC
DCILGGDDLTVSAVAQGRDAAESIHRSLIA
EEEECCCCCEEEEHHCCCHHHHHHHHHHCC
>Mature Secondary Structure 
AQRHYEDGIVSGRFSPEDYAQNFSDLHPALDTHEARVEADRCYFCYDAPCMRACPTTID
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHCCCCCEEEEECCCHHHHCCCCCC
IPLFIRQISTGNPLGAARTIFDQNIFGGMCARVCPTETLCEEACVREHAEGKPVQIGRLQ
HHHHEEECCCCCCCCHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEHHHHH
RFATDAAMARGKRFYTRAAASGKKVAVVGAGPAGLACAHRLAMYGHEVTVYEARPKPGGL
HHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCEEEEEECCCCCCCC
NEYGIAAYKTPGGFAQAEVDYILSIGGITIEHGKALGRDFTLAGLAAGSDAVFLAMGLAG
CCCCEEEEECCCCCCHHCEEEEEEECCEEEECCCCCCCCEEEEEECCCCCCEEEEHHHHC
VNALRCEGEEADGVENAVDYIATLRQADDLSKLPIGGRVVVIGGGMTAIDAAIQSRLLGA
CCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCC
EEVTICYRRGKEHMNASEWEQELAAAKGVTIRHWMQPRRVIAEANRVSGIEMEYTAIVDG
HHHHHHHHCCHHHCCHHHHHHHHHHHCCCCHHHHHCHHHHHHHHHHCCCCEEEEEEEECC
RLIGTGDTLILKADQIFKAIGQSFDPAVLNGGGDAIVFEKDRIRVDGEGRTSMPKVWAGG
EEEECCCEEEEEHHHHHHHHCCCCCCEEEECCCCEEEEECCEEEECCCCCCCCCEEEECC
DCILGGDDLTVSAVAQGRDAAESIHRSLIA
EEEECCCCCEEEEHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]