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The map label for this gene is ilvB [H]

Identifier: 110634387

GI number: 110634387

Start: 2189687

End: 2191336

Strand: Direct

Name: ilvB [H]

Synonym: Meso_2037

Alternate gene names: 110634387

Gene position: 2189687-2191336 (Clockwise)

Preceding gene: 110634386

Following gene: 110634392

Centisome position: 49.63

GC content: 65.39

Gene sequence:

>1650_bases
GTGAAGAGCGGCGGTCAACTCATCGTCGAAGCCTTGGAAGCAAATGGCGTGGATCGCCTCTACTGCGTGCCGGGCGAAAG
CTATCTGGCAGTGCTCGACGCGCTCCACGATTCCAAGATCCGCACCATCGTCTGCCGCCAGGAAGGCGGCGCGGCCATGA
TGGCCGATTGCCACGGAAGGCTCACCGGCCGCCCCGGCATCTGCTTCGTCACGCGTGGGCCAGGCGCCACGAACGCGACG
GCCGGCATCCATATCGCAAGGCAGGACTCGGTGCCGATGATCCTTTTCATCGGCCAGGTGGCAAGCCATGCCCGCGAGCG
CGAGGCCTTTCAGGAAGTGGATTACCGCGCCCTGTTCGGGCCGCTCGCCAAATGGGCGACGGAAATCGACGATCCGGCCC
GCATTCCCGAAATCGTCACGCGCGCCTTTTCGGTCGCCACCTCCGGCCGGCCCGGCCCGGTGGTCATCTCCCTTCCCGAG
GACATGCTGACGGAAACGGCTGAGGCGCCCGCCGCCCTGCCCTATACGCCGGTGGAAACCCGCCCCGGCCATCACGAGAT
GCAGAAGCTGGAAGAGCTTCTGGGCACAGCGAAACGTCCCTTCGTCATCCTTGGCGGCACCCGCTGGGACGAAGCCTCCG
TGTCGGCCTTCCAGAAGGCGGCGGAGCGCTGGGCGCTTCCCGTAGGCGTCTCCTTCCGCCGCCAGATGCTGTTCGATCAC
CTGCACCCCTGCTATGCAGGCGATATCGGCATCGGCCCGAACCCGAAGCTGGCCGAGGCGATCAAGAGCGCCGATCTCGT
GCTGCTCGTCGGCGGACGTTTCGGGGAGATCCCGTCTTCAGATTACACGCTTCTGAAGAGCCCCTACCCCGACCAGAAGC
TGGTGCATGTGCACCCCGACGCGAACGAACTCGGCCGCGTCTACCGCCCGACGCTTGCCATCAATGCCTCGCCCGCGGCC
TTCGCCGCGGCTTTCGCGGAACTCGATCCCGGCGCCACGCCCGACTGGGCCGATGAGACGACGCGACTCCACGGCGCCTA
TCTCACATGGTCGACACCGCCTGAGACCGGCCCCGGCGCCGTGCATATGGGACCGATTTGGAACCACCTCGAAAGCGTCT
TGCCGGAGGATGCGATCATCACCAACGGCGCGGGCAACTACGCGACCTGGATCCACCGCTTCCACCGCTTCCGCCGCTAT
GGCACGCAGAGCGCGCCCACATCGGGCTCGATGGGCTACGGCACGCCGGCCGCGGTGGCGGCAAAGGATCTTTTCCCAGA
CCGCCTCGTGGTGGCGTTCGCGGGCGACGGCTGTTTCCTCATGAACGGGCAGGAATTTGCCACCGCCGTGCAACATGACC
TGCCCATCATCGTGATCGTCGTCAATAACGGGACCTATGGCACAATCCGCATGCACCAGGAGCGCGAATATCCCGGCCGT
GTCTCCGGCACCGACTTGAAGAACCCTGACTTTGCGGCGCTCGCCCGCGCCTATGGCGGCTATGGCGAGACGGTGGAGAA
GACGGAGGATTTCGCGCCTGCCTTCGAGCGCGCGCGGGAAAGCGGAAAGCCGGCGATCATTGAGATAAAGCTCGACCCGG
AAGCGATCACGCCGACAAAGACGCTGACGCAGATCCGTGAGGGGCGATAA

Upstream 100 bases:

>100_bases
CGCCGATGGTCATGTCGCAAACCCCGCTTGCCTATACGCGCCCAAGCCCGCGGCTGGGCGAACATACCGAGGAAATCCTG
CGCGAACTGGAGGAAAGCAC

Downstream 100 bases:

>100_bases
TTCTTCTGACCTCAGGGGATGTGAGCGCCGCCTAACCGCCTCTTCTGGCATGATAGGCGGCGCCGGCGGCCGCCCCGAGT
GCAATCCCGACGGCTATTCC

Product: thiamine pyrophosphate protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MKSGGQLIVEALEANGVDRLYCVPGESYLAVLDALHDSKIRTIVCRQEGGAAMMADCHGRLTGRPGICFVTRGPGATNAT
AGIHIARQDSVPMILFIGQVASHAREREAFQEVDYRALFGPLAKWATEIDDPARIPEIVTRAFSVATSGRPGPVVISLPE
DMLTETAEAPAALPYTPVETRPGHHEMQKLEELLGTAKRPFVILGGTRWDEASVSAFQKAAERWALPVGVSFRRQMLFDH
LHPCYAGDIGIGPNPKLAEAIKSADLVLLVGGRFGEIPSSDYTLLKSPYPDQKLVHVHPDANELGRVYRPTLAINASPAA
FAAAFAELDPGATPDWADETTRLHGAYLTWSTPPETGPGAVHMGPIWNHLESVLPEDAIITNGAGNYATWIHRFHRFRRY
GTQSAPTSGSMGYGTPAAVAAKDLFPDRLVVAFAGDGCFLMNGQEFATAVQHDLPIIVIVVNNGTYGTIRMHQEREYPGR
VSGTDLKNPDFAALARAYGGYGETVEKTEDFAPAFERARESGKPAIIEIKLDPEAITPTKTLTQIREGR

Sequences:

>Translated_549_residues
MKSGGQLIVEALEANGVDRLYCVPGESYLAVLDALHDSKIRTIVCRQEGGAAMMADCHGRLTGRPGICFVTRGPGATNAT
AGIHIARQDSVPMILFIGQVASHAREREAFQEVDYRALFGPLAKWATEIDDPARIPEIVTRAFSVATSGRPGPVVISLPE
DMLTETAEAPAALPYTPVETRPGHHEMQKLEELLGTAKRPFVILGGTRWDEASVSAFQKAAERWALPVGVSFRRQMLFDH
LHPCYAGDIGIGPNPKLAEAIKSADLVLLVGGRFGEIPSSDYTLLKSPYPDQKLVHVHPDANELGRVYRPTLAINASPAA
FAAAFAELDPGATPDWADETTRLHGAYLTWSTPPETGPGAVHMGPIWNHLESVLPEDAIITNGAGNYATWIHRFHRFRRY
GTQSAPTSGSMGYGTPAAVAAKDLFPDRLVVAFAGDGCFLMNGQEFATAVQHDLPIIVIVVNNGTYGTIRMHQEREYPGR
VSGTDLKNPDFAALARAYGGYGETVEKTEDFAPAFERARESGKPAIIEIKLDPEAITPTKTLTQIREGR
>Mature_549_residues
MKSGGQLIVEALEANGVDRLYCVPGESYLAVLDALHDSKIRTIVCRQEGGAAMMADCHGRLTGRPGICFVTRGPGATNAT
AGIHIARQDSVPMILFIGQVASHAREREAFQEVDYRALFGPLAKWATEIDDPARIPEIVTRAFSVATSGRPGPVVISLPE
DMLTETAEAPAALPYTPVETRPGHHEMQKLEELLGTAKRPFVILGGTRWDEASVSAFQKAAERWALPVGVSFRRQMLFDH
LHPCYAGDIGIGPNPKLAEAIKSADLVLLVGGRFGEIPSSDYTLLKSPYPDQKLVHVHPDANELGRVYRPTLAINASPAA
FAAAFAELDPGATPDWADETTRLHGAYLTWSTPPETGPGAVHMGPIWNHLESVLPEDAIITNGAGNYATWIHRFHRFRRY
GTQSAPTSGSMGYGTPAAVAAKDLFPDRLVVAFAGDGCFLMNGQEFATAVQHDLPIIVIVVNNGTYGTIRMHQEREYPGR
VSGTDLKNPDFAALARAYGGYGETVEKTEDFAPAFERARESGKPAIIEIKLDPEAITPTKTLTQIREGR

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=562, Percent_Identity=23.8434163701068, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI21361361, Length=580, Percent_Identity=24.6551724137931, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI87081685, Length=576, Percent_Identity=30.2083333333333, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI1790104, Length=553, Percent_Identity=30.1989150090416, Blast_Score=228, Evalue=1e-60,
Organism=Escherichia coli, GI1786717, Length=552, Percent_Identity=27.1739130434783, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1787096, Length=559, Percent_Identity=25.2236135957066, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1788716, Length=577, Percent_Identity=26.6897746967071, Blast_Score=123, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17542570, Length=565, Percent_Identity=24.4247787610619, Blast_Score=128, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17531301, Length=568, Percent_Identity=26.4084507042254, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17531299, Length=565, Percent_Identity=26.1946902654867, Blast_Score=121, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6323755, Length=555, Percent_Identity=26.8468468468468, Blast_Score=216, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6320816, Length=565, Percent_Identity=21.4159292035398, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6323163, Length=488, Percent_Identity=23.9754098360656, Blast_Score=93, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6323073, Length=504, Percent_Identity=23.6111111111111, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19922626, Length=585, Percent_Identity=26.4957264957265, Blast_Score=150, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59450; Mature: 59450

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSGGQLIVEALEANGVDRLYCVPGESYLAVLDALHDSKIRTIVCRQEGGAAMMADCHGR
CCCCHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCC
LTGRPGICFVTRGPGATNATAGIHIARQDSVPMILFIGQVASHAREREAFQEVDYRALFG
CCCCCCEEEEECCCCCCCCCCCEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHH
PLAKWATEIDDPARIPEIVTRAFSVATSGRPGPVVISLPEDMLTETAEAPAALPYTPVET
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHCCCCCCCCCCCCC
RPGHHEMQKLEELLGTAKRPFVILGGTRWDEASVSAFQKAAERWALPVGVSFRRQMLFDH
CCCHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LHPCYAGDIGIGPNPKLAEAIKSADLVLLVGGRFGEIPSSDYTLLKSPYPDQKLVHVHPD
CCCCCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCEEEECCC
ANELGRVYRPTLAINASPAAFAAAFAELDPGATPDWADETTRLHGAYLTWSTPPETGPGA
HHHHHHHHCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCC
VHMGPIWNHLESVLPEDAIITNGAGNYATWIHRFHRFRRYGTQSAPTSGSMGYGTPAAVA
EEECHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
AKDLFPDRLVVAFAGDGCFLMNGQEFATAVQHDLPIIVIVVNNGTYGTIRMHQEREYPGR
HHHCCCCEEEEEEECCCEEEECCHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCCCCC
VSGTDLKNPDFAALARAYGGYGETVEKTEDFAPAFERARESGKPAIIEIKLDPEAITPTK
CCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHH
TLTQIREGR
HHHHHHCCC
>Mature Secondary Structure
MKSGGQLIVEALEANGVDRLYCVPGESYLAVLDALHDSKIRTIVCRQEGGAAMMADCHGR
CCCCHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCC
LTGRPGICFVTRGPGATNATAGIHIARQDSVPMILFIGQVASHAREREAFQEVDYRALFG
CCCCCCEEEEECCCCCCCCCCCEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHH
PLAKWATEIDDPARIPEIVTRAFSVATSGRPGPVVISLPEDMLTETAEAPAALPYTPVET
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHCCCCCCCCCCCCC
RPGHHEMQKLEELLGTAKRPFVILGGTRWDEASVSAFQKAAERWALPVGVSFRRQMLFDH
CCCHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LHPCYAGDIGIGPNPKLAEAIKSADLVLLVGGRFGEIPSSDYTLLKSPYPDQKLVHVHPD
CCCCCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCEEEECCC
ANELGRVYRPTLAINASPAAFAAAFAELDPGATPDWADETTRLHGAYLTWSTPPETGPGA
HHHHHHHHCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCC
VHMGPIWNHLESVLPEDAIITNGAGNYATWIHRFHRFRRYGTQSAPTSGSMGYGTPAAVA
EEECHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
AKDLFPDRLVVAFAGDGCFLMNGQEFATAVQHDLPIIVIVVNNGTYGTIRMHQEREYPGR
HHHCCCCEEEEEEECCCEEEECCHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCCCCC
VSGTDLKNPDFAALARAYGGYGETVEKTEDFAPAFERARESGKPAIIEIKLDPEAITPTK
CCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHH
TLTQIREGR
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8366043; 12948626 [H]