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The map label for this gene is metF [H]

Identifier: 110634378

GI number: 110634378

Start: 2180890

End: 2181807

Strand: Reverse

Name: metF [H]

Synonym: Meso_2028

Alternate gene names: 110634378

Gene position: 2181807-2180890 (Counterclockwise)

Preceding gene: 110634379

Following gene: 110634377

Centisome position: 49.45

GC content: 63.29

Gene sequence:

>918_bases
ATGACGAACTACCGATTCGCGCGGCGGCACGAGGCGGCCGAGAAGATCCGCGTATCCTTTGAATTTTTCCCGCCGAAGAG
CGATGAGATGGCGGAAAAGCTGTGGGAGACCGTAGTGCGTCTCGCCCCGCTCCAGCCCGCTTTCGTGTCGGTCACTTATG
GTGCCGGCGGCTCGACGCGCGAGCGAACGGCCCATACGGTGCGCCGGATCCTGCAGGAGACGGAGCTGACCCCGGCGGCG
CACCTTACCTGCGTGGATGCTTCGCGCGAGCAGGTCGATGGCGTCATTTCCGAATTCGCCGCCATGGGCGTGAAACGTTT
CGTGGCGCTGCGCGGCGATCCCGTGGAAGGTGCCGGTGCAGCCTATCGGCCACATCCGGACGGCTACATCAACGGGGCGG
ACCTCGTCGGCGCGCTGGCCCGTCAAGGAGATTTCGACATCTCGGTTTCCGCCTACCCGGAAAAGCATCCGGAAAGTCCC
GACTTCGCAACCGATATCGAGATGCTCAAGCGCAAGGTGGACAATGGCGCCACGCGCGCGATCACCCAATTCTTCTTCGA
CAACGACGTCTATGATCGATACGTGGAGCGCGTGCGCCGGGCAGGCATTTACGTGCCGATCGTGCCGGGAATCCTGCCCG
TCCATAATTTTACGCAGGTTGTCCGCTTCGCAAGCCGCTGCGGCGCGTATGTGCCGCCTTGGCTGGCCGAGCGCTTTCAT
GGCCTGGAGAACGATCCCTCGACGCATCAGCTGGTGGCGGCGGCGGTCGCGGCCGAACAGGTGCTGGACCTCGTTGAACG
CGGCGTCAGCGATTTCCACTTCTATACGATGAACCGGGCTGACCTTCCTTTTGCCATCTGCCACATGATCGGCGTCAGGC
CGGCGGCGCAGCCTTCAGGCGAGGTAGCCGTCGCCTAA

Upstream 100 bases:

>100_bases
GCGGGACAAAGAAGGGACTGACAGTGAAAATGTGGCTGGCCCACGACCGGCGCGTCGCAGTGGCATCAGAAGAAATCACC
GCGCGCAAGGAGATGGCGTG

Downstream 100 bases:

>100_bases
AAAAACGGAATTGAAAAAGGCCGGGTTTTCACCCGGCCTTCCCGTCTATTCAGCTTTGGCTCTTGCGGCTGTAGCTGCTT
ACGGCAGGACCCCCATGATG

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MTNYRFARRHEAAEKIRVSFEFFPPKSDEMAEKLWETVVRLAPLQPAFVSVTYGAGGSTRERTAHTVRRILQETELTPAA
HLTCVDASREQVDGVISEFAAMGVKRFVALRGDPVEGAGAAYRPHPDGYINGADLVGALARQGDFDISVSAYPEKHPESP
DFATDIEMLKRKVDNGATRAITQFFFDNDVYDRYVERVRRAGIYVPIVPGILPVHNFTQVVRFASRCGAYVPPWLAERFH
GLENDPSTHQLVAAAVAAEQVLDLVERGVSDFHFYTMNRADLPFAICHMIGVRPAAQPSGEVAVA

Sequences:

>Translated_305_residues
MTNYRFARRHEAAEKIRVSFEFFPPKSDEMAEKLWETVVRLAPLQPAFVSVTYGAGGSTRERTAHTVRRILQETELTPAA
HLTCVDASREQVDGVISEFAAMGVKRFVALRGDPVEGAGAAYRPHPDGYINGADLVGALARQGDFDISVSAYPEKHPESP
DFATDIEMLKRKVDNGATRAITQFFFDNDVYDRYVERVRRAGIYVPIVPGILPVHNFTQVVRFASRCGAYVPPWLAERFH
GLENDPSTHQLVAAAVAAEQVLDLVERGVSDFHFYTMNRADLPFAICHMIGVRPAAQPSGEVAVA
>Mature_304_residues
TNYRFARRHEAAEKIRVSFEFFPPKSDEMAEKLWETVVRLAPLQPAFVSVTYGAGGSTRERTAHTVRRILQETELTPAAH
LTCVDASREQVDGVISEFAAMGVKRFVALRGDPVEGAGAAYRPHPDGYINGADLVGALARQGDFDISVSAYPEKHPESPD
FATDIEMLKRKVDNGATRAITQFFFDNDVYDRYVERVRRAGIYVPIVPGILPVHNFTQVVRFASRCGAYVPPWLAERFHG
LENDPSTHQLVAAAVAAEQVLDLVERGVSDFHFYTMNRADLPFAICHMIGVRPAAQPSGEVAVA

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=282, Percent_Identity=31.5602836879433, Blast_Score=139, Evalue=4e-33,
Organism=Escherichia coli, GI1790377, Length=285, Percent_Identity=48.0701754385965, Blast_Score=275, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI25147441, Length=269, Percent_Identity=26.7657992565056, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI25147436, Length=269, Percent_Identity=26.7657992565056, Blast_Score=113, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321313, Length=301, Percent_Identity=28.9036544850498, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6325234, Length=292, Percent_Identity=31.1643835616438, Blast_Score=132, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24662658, Length=260, Percent_Identity=28.4615384615385, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 33791; Mature: 33659

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNYRFARRHEAAEKIRVSFEFFPPKSDEMAEKLWETVVRLAPLQPAFVSVTYGAGGSTR
CCCCCHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCH
ERTAHTVRRILQETELTPAAHLTCVDASREQVDGVISEFAAMGVKRFVALRGDPVEGAGA
HHHHHHHHHHHHHHCCCCHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
AYRPHPDGYINGADLVGALARQGDFDISVSAYPEKHPESPDFATDIEMLKRKVDNGATRA
CCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHH
ITQFFFDNDVYDRYVERVRRAGIYVPIVPGILPVHNFTQVVRFASRCGAYVPPWLAERFH
HHHHHHCCCHHHHHHHHHHHCCCEEEECCCHHCHHHHHHHHHHHHHCCCCCCHHHHHHHC
GLENDPSTHQLVAAAVAAEQVLDLVERGVSDFHFYTMNRADLPFAICHMIGVRPAAQPSG
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCC
EVAVA
CEECC
>Mature Secondary Structure 
TNYRFARRHEAAEKIRVSFEFFPPKSDEMAEKLWETVVRLAPLQPAFVSVTYGAGGSTR
CCCCHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCH
ERTAHTVRRILQETELTPAAHLTCVDASREQVDGVISEFAAMGVKRFVALRGDPVEGAGA
HHHHHHHHHHHHHHCCCCHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
AYRPHPDGYINGADLVGALARQGDFDISVSAYPEKHPESPDFATDIEMLKRKVDNGATRA
CCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHH
ITQFFFDNDVYDRYVERVRRAGIYVPIVPGILPVHNFTQVVRFASRCGAYVPPWLAERFH
HHHHHHCCCHHHHHHHHHHHCCCEEEECCCHHCHHHHHHHHHHHHHCCCCCCHHHHHHHC
GLENDPSTHQLVAAAVAAEQVLDLVERGVSDFHFYTMNRADLPFAICHMIGVRPAAQPSG
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCC
EVAVA
CEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA