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The map label for this gene is ligA

Identifier: 110634347

GI number: 110634347

Start: 2139879

End: 2141990

Strand: Reverse

Name: ligA

Synonym: Meso_1997

Alternate gene names: 110634347

Gene position: 2141990-2139879 (Counterclockwise)

Preceding gene: 110634348

Following gene: 110634346

Centisome position: 48.54

GC content: 62.36

Gene sequence:

>2112_bases
ATGACCACAGCCAGGATCCCCGTCGATGAACTGACGGAGGAGCAAGCCAAGGACGAACTTGCCCGTCTTGCGCAGGAGAT
CGGGAGGCATGACCGGCTCTATTACTCAGAGGACGCGCCGGAAGTCTCCGATGCCGAATATGACGCGTTGCGGCGGCGCA
ATCTGGCCATCGAGCAGGCGTTCCCGAATCTGATCCGGGCGGATTCGCCGTCGAAGCGAATCGGAGCACCGGCAGTCGAG
AAATTCGAGAAGGTCCGGCACAAGGTGCCGATGCTCTCGCTGGACAACGCTTTCACCGATGACGACGTCGTCGACTTCGT
GGCGCGTGTCCGCCGCTTTCTGAAGATGGAGCCCCATGCGCCGATCGCCATGACGGCGGAGCCGAAGATCGATGGGCTGT
CCCTTTCGATTCGCTATGAAAACGGGAGGCTCGTCTCGGCTGCAACGCGCGGCGACGGGCAGGTGGGCGAGAATGTCACC
GCCAATGCCCGCGCCGTTGCCGATATTCCTAATGTGCTTTCCGGCGATTTTCCCGAGATCCTGGAAGTGCGCGGCGAGGT
CTACATGACCCATCGCGATTTTCTGGCGCTCAACGAACGCCAGCGCGCGGAGGGCAAGCAGACCTATGTGAACCCGCGCA
ACACGGCGGCAGGATCGCTCCGCCAGCTGGACGCGTCGATCACGGCGGCGCGACCGCTCAAATTCTTCGCCTATGCATGG
GGCGAGGTGAGCGAGATGCCGTCTCAGACCCAAATGGGAATGGTCGAAGCGCTGAAAAGCTACGGCTTTCGGACCAACAA
GCTGATGTGCCTTTGCGAGACTGCGCGAGAGCTGCTGGAGCACTATCATGCCATCGAGGAGCGCCGCGCCACGCTGGGCT
ATGATATCGACGGCGTCGTTTACAAGGTGAACGATCTGGCGCTGCAGCAGCGGTTGGGATACGTCTCCCGCAGCCCGCGC
TGGGCCATTGCGCATAAGTTTGCTGCCGAGAAGGCGTTCACCATTCTGCGCGATATCGACATCCAGGTGGGCCGCACCGG
TGCGCTGACGCCTGTGGCGCGGCTGGAGCCAGTCACGGTGGGCGGCGTGGTGGTGACAAACGCCACGCTGCACAATGCCG
AGGAGATCGAACGCCTCGGAATCAAAATCGGCGATACGGTCCAGGTGCAGCGTGCGGGCGATGTCATTCCGCAGATTCTC
GGTTATGCGGAGGATCGCAGACCAGCGGATGCGAAAGAATTCGTCTTCCCCAGCGTCTGCCCCTGCGAGCTCAAGACGCC
GGTCGTGCGCGATTCCACGGCGGGAGGCGGGGAGGGCGTGGTGCGGCGCTGCTCGGGCGAGTTTGCCTGTCCCTTCCAGC
GCATCGAACATCTGCGTCTCTTCGTCTCCCGGCGCGCTTTCGATATCGAGGGATTGGGCGAAAAGCAGATCGAGTTCTTC
TTCCGCGACCCGGACCTGCCGGTCAAAAGCCCCGCAGACATCTTCACACTTGCCGAGCGCGACGCGCAGAACATCAAAAA
GCTCAAGGACAAGGAAGGGTGGGGCACGGTCTCGGCCGGAAATCTCTTCGCGGCCATCGAAGCAAAGCGGACTATCCCGC
TCGACCGCATGATCTATGGGCTGGGTATCCGTCACGTGGGTGAGCGCACCGCCGTTACGCTGGCGCGCGGATATGGTTCA
TGGAAGGCCTTCCACGATTCGGCTCTTGCGATTGCCGGCGGAGATCTCTCCGCACGGGAAGAGATGGACGCGCTGGAGGA
TATCGGCGATGCCGTCATAGACGCGATCGCCACTTATTTCGCAGAGGAGCACAATTGCCGGCTTGTGGAGGCGCTCGCCG
CGCAGGTCAACGTGCTGGAGGCGGAGCGGCCGGTTGCCGATTCGCCGGTGGCGGGCAAGACGATCGTCTTTACCGGCGCG
CTCGAACGCATGTCGCGCGACGAGGCGAAAGACATGGCCGAGCGGTTGGGAGCCAAGGTGGCCGGTTCGGTCTCCTCAAA
GACGGATCTGGTCGTGGCAGGGCCCGGCGCTGGCTCCAAGCTCAAAAAGGCAGCCGAGCTCGGCATTGAGGTCATCGACG
AGGATATGTGGTTCCAGAGGATCGGTGCATAG

Upstream 100 bases:

>100_bases
CCGACTTGTCATTCCATGGCGGTATAGGCGGTGCGAACCGCTTGAGCGTCTGGCCGCAAACGCCATATGTTGGCAGCCAA
ACGAATCCGGATTGCCGCCC

Downstream 100 bases:

>100_bases
GACGATACATCACCGCTGTTCATCCTCCCCTCAAACGAATTGATGTGACGGGTCAGTATCACTGACCTACCTTCCGCGCG
GAGGTTTCAATGAGTGCAGA

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 703; Mature: 702

Protein sequence:

>703_residues
MTTARIPVDELTEEQAKDELARLAQEIGRHDRLYYSEDAPEVSDAEYDALRRRNLAIEQAFPNLIRADSPSKRIGAPAVE
KFEKVRHKVPMLSLDNAFTDDDVVDFVARVRRFLKMEPHAPIAMTAEPKIDGLSLSIRYENGRLVSAATRGDGQVGENVT
ANARAVADIPNVLSGDFPEILEVRGEVYMTHRDFLALNERQRAEGKQTYVNPRNTAAGSLRQLDASITAARPLKFFAYAW
GEVSEMPSQTQMGMVEALKSYGFRTNKLMCLCETARELLEHYHAIEERRATLGYDIDGVVYKVNDLALQQRLGYVSRSPR
WAIAHKFAAEKAFTILRDIDIQVGRTGALTPVARLEPVTVGGVVVTNATLHNAEEIERLGIKIGDTVQVQRAGDVIPQIL
GYAEDRRPADAKEFVFPSVCPCELKTPVVRDSTAGGGEGVVRRCSGEFACPFQRIEHLRLFVSRRAFDIEGLGEKQIEFF
FRDPDLPVKSPADIFTLAERDAQNIKKLKDKEGWGTVSAGNLFAAIEAKRTIPLDRMIYGLGIRHVGERTAVTLARGYGS
WKAFHDSALAIAGGDLSAREEMDALEDIGDAVIDAIATYFAEEHNCRLVEALAAQVNVLEAERPVADSPVAGKTIVFTGA
LERMSRDEAKDMAERLGAKVAGSVSSKTDLVVAGPGAGSKLKKAAELGIEVIDEDMWFQRIGA

Sequences:

>Translated_703_residues
MTTARIPVDELTEEQAKDELARLAQEIGRHDRLYYSEDAPEVSDAEYDALRRRNLAIEQAFPNLIRADSPSKRIGAPAVE
KFEKVRHKVPMLSLDNAFTDDDVVDFVARVRRFLKMEPHAPIAMTAEPKIDGLSLSIRYENGRLVSAATRGDGQVGENVT
ANARAVADIPNVLSGDFPEILEVRGEVYMTHRDFLALNERQRAEGKQTYVNPRNTAAGSLRQLDASITAARPLKFFAYAW
GEVSEMPSQTQMGMVEALKSYGFRTNKLMCLCETARELLEHYHAIEERRATLGYDIDGVVYKVNDLALQQRLGYVSRSPR
WAIAHKFAAEKAFTILRDIDIQVGRTGALTPVARLEPVTVGGVVVTNATLHNAEEIERLGIKIGDTVQVQRAGDVIPQIL
GYAEDRRPADAKEFVFPSVCPCELKTPVVRDSTAGGGEGVVRRCSGEFACPFQRIEHLRLFVSRRAFDIEGLGEKQIEFF
FRDPDLPVKSPADIFTLAERDAQNIKKLKDKEGWGTVSAGNLFAAIEAKRTIPLDRMIYGLGIRHVGERTAVTLARGYGS
WKAFHDSALAIAGGDLSAREEMDALEDIGDAVIDAIATYFAEEHNCRLVEALAAQVNVLEAERPVADSPVAGKTIVFTGA
LERMSRDEAKDMAERLGAKVAGSVSSKTDLVVAGPGAGSKLKKAAELGIEVIDEDMWFQRIGA
>Mature_702_residues
TTARIPVDELTEEQAKDELARLAQEIGRHDRLYYSEDAPEVSDAEYDALRRRNLAIEQAFPNLIRADSPSKRIGAPAVEK
FEKVRHKVPMLSLDNAFTDDDVVDFVARVRRFLKMEPHAPIAMTAEPKIDGLSLSIRYENGRLVSAATRGDGQVGENVTA
NARAVADIPNVLSGDFPEILEVRGEVYMTHRDFLALNERQRAEGKQTYVNPRNTAAGSLRQLDASITAARPLKFFAYAWG
EVSEMPSQTQMGMVEALKSYGFRTNKLMCLCETARELLEHYHAIEERRATLGYDIDGVVYKVNDLALQQRLGYVSRSPRW
AIAHKFAAEKAFTILRDIDIQVGRTGALTPVARLEPVTVGGVVVTNATLHNAEEIERLGIKIGDTVQVQRAGDVIPQILG
YAEDRRPADAKEFVFPSVCPCELKTPVVRDSTAGGGEGVVRRCSGEFACPFQRIEHLRLFVSRRAFDIEGLGEKQIEFFF
RDPDLPVKSPADIFTLAERDAQNIKKLKDKEGWGTVSAGNLFAAIEAKRTIPLDRMIYGLGIRHVGERTAVTLARGYGSW
KAFHDSALAIAGGDLSAREEMDALEDIGDAVIDAIATYFAEEHNCRLVEALAAQVNVLEAERPVADSPVAGKTIVFTGAL
ERMSRDEAKDMAERLGAKVAGSVSSKTDLVVAGPGAGSKLKKAAELGIEVIDEDMWFQRIGA

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=699, Percent_Identity=47.6394849785408, Blast_Score=586, Evalue=1e-168,
Organism=Escherichia coli, GI87082305, Length=389, Percent_Identity=23.9074550128535, Blast_Score=94, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_MESSB (Q11GT5)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_674555.1
- ProteinModelPortal:   Q11GT5
- STRING:   Q11GT5
- GeneID:   4182084
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_1997
- NMPDR:   fig|266779.1.peg.592
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   Q11GT5
- BioCyc:   MSP266779:MESO_1997-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 77414; Mature: 77283

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 129-129 BINDING 127-127 BINDING 150-150 BINDING 186-186 BINDING 302-302 BINDING 326-326

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTARIPVDELTEEQAKDELARLAQEIGRHDRLYYSEDAPEVSDAEYDALRRRNLAIEQA
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHCCHHHHH
FPNLIRADSPSKRIGAPAVEKFEKVRHKVPMLSLDNAFTDDDVVDFVARVRRFLKMEPHA
CCHHHCCCCCCCCCCCHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCC
PIAMTAEPKIDGLSLSIRYENGRLVSAATRGDGQVGENVTANARAVADIPNVLSGDFPEI
CEEEECCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCCCCCCHHHHHCCHHHCCCCHHH
LEVRGEVYMTHRDFLALNERQRAEGKQTYVNPRNTAAGSLRQLDASITAARPLKFFAYAW
HHHCCCEEEEEHHHHHHCCHHHCCCCCEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHH
GEVSEMPSQTQMGMVEALKSYGFRTNKLMCLCETARELLEHYHAIEERRATLGYDIDGVV
HHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
YKVNDLALQQRLGYVSRSPRWAIAHKFAAEKAFTILRDIDIQVGRTGALTPVARLEPVTV
EEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCHHHCCCEEE
GGVVVTNATLHNAEEIERLGIKIGDTVQVQRAGDVIPQILGYAEDRRPADAKEFVFPSVC
CCEEEECCCCCCHHHHHHHCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCC
PCELKTPVVRDSTAGGGEGVVRRCSGEFACPFQRIEHLRLFVSRRAFDIEGLGEKQIEFF
CCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
FRDPDLPVKSPADIFTLAERDAQNIKKLKDKEGWGTVSAGNLFAAIEAKRTIPLDRMIYG
EECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCEEEEEECCCCCCHHHHHHH
LGIRHVGERTAVTLARGYGSWKAFHDSALAIAGGDLSAREEMDALEDIGDAVIDAIATYF
HHHHHCCCHHEEEEECCCCCCHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
AEEHNCRLVEALAAQVNVLEAERPVADSPVAGKTIVFTGALERMSRDEAKDMAERLGAKV
HCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCHH
AGSVSSKTDLVVAGPGAGSKLKKAAELGIEVIDEDMWFQRIGA
HCCCCCCCCEEEECCCCCHHHHHHHHHCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTARIPVDELTEEQAKDELARLAQEIGRHDRLYYSEDAPEVSDAEYDALRRRNLAIEQA
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHCCHHHHH
FPNLIRADSPSKRIGAPAVEKFEKVRHKVPMLSLDNAFTDDDVVDFVARVRRFLKMEPHA
CCHHHCCCCCCCCCCCHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCC
PIAMTAEPKIDGLSLSIRYENGRLVSAATRGDGQVGENVTANARAVADIPNVLSGDFPEI
CEEEECCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCCCCCCHHHHHCCHHHCCCCHHH
LEVRGEVYMTHRDFLALNERQRAEGKQTYVNPRNTAAGSLRQLDASITAARPLKFFAYAW
HHHCCCEEEEEHHHHHHCCHHHCCCCCEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHH
GEVSEMPSQTQMGMVEALKSYGFRTNKLMCLCETARELLEHYHAIEERRATLGYDIDGVV
HHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
YKVNDLALQQRLGYVSRSPRWAIAHKFAAEKAFTILRDIDIQVGRTGALTPVARLEPVTV
EEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCHHHCCCEEE
GGVVVTNATLHNAEEIERLGIKIGDTVQVQRAGDVIPQILGYAEDRRPADAKEFVFPSVC
CCEEEECCCCCCHHHHHHHCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCC
PCELKTPVVRDSTAGGGEGVVRRCSGEFACPFQRIEHLRLFVSRRAFDIEGLGEKQIEFF
CCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
FRDPDLPVKSPADIFTLAERDAQNIKKLKDKEGWGTVSAGNLFAAIEAKRTIPLDRMIYG
EECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCEEEEEECCCCCCHHHHHHH
LGIRHVGERTAVTLARGYGSWKAFHDSALAIAGGDLSAREEMDALEDIGDAVIDAIATYF
HHHHHCCCHHEEEEECCCCCCHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
AEEHNCRLVEALAAQVNVLEAERPVADSPVAGKTIVFTGALERMSRDEAKDMAERLGAKV
HCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCHH
AGSVSSKTDLVVAGPGAGSKLKKAAELGIEVIDEDMWFQRIGA
HCCCCCCCCEEEECCCCCHHHHHHHHHCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA