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The map label for this gene is potB [H]

Identifier: 110634213

GI number: 110634213

Start: 2002265

End: 2003122

Strand: Reverse

Name: potB [H]

Synonym: Meso_1863

Alternate gene names: 110634213

Gene position: 2003122-2002265 (Counterclockwise)

Preceding gene: 110634214

Following gene: 110634212

Centisome position: 45.4

GC content: 59.44

Gene sequence:

>858_bases
ATGAACCAGGGTTTCCAGGCGATCAAGGCCCAGGCAGGCTCACTGGCGCTTGTAAGTCCCGCCCTCCTGCTGCTCGGGAT
CCTGTTCTTCTACCCGATCCTTCTTTTGTTCTTAACGACCCTCGAACTGGAGAACATCGGCAATTATGCGCAGGTGGTGA
GCAGCGACACCTCCATGCAGGTTTTCTTCAACACGCTGCGCATCGCCGTCATCGTCACGGTAGTCGATCTGGTCCTCGCA
TATCCGCTGGCGGTCTACATCTCGCGCCTGTCGCCGACAGCAAAGACGTTTGCCTTTTTCCTGGTGCTGATCCCGCTGTG
GACGAGCCAGCTCGTGCGCACCTATGCCTGGATGGTCCTTCTCGGGCGCAACGGACCGATCAACAACGCGCTTGTCTGGC
TGGGCATAGTCGATGCGCCGATCAGGCTGGCCAACAGCGAGTTCGCGGTCATCGCCGGCATGACGCATATCCTGCTGCCA
TACATGCTGCTGCCGATCTACAACTCAGTTTCCAAAATCGATCCATCGCTCTTCGACGCGGCGCGCGGGCTCGGCGCCGG
AGCCTTCTCGACCTTTACGCGGGTGCTGATGCCGCTGAGCATCCAAGGTGTCCTGGCGGGGGTCAGCCTTGTGCTGGTGC
TTTCGCTCGGCGTGTTCATCATGCCGGCCTTGCTGGGCGGTGGCCGGGTTCCCGTCATTGCGCTCCTCATCGAGCAGCAG
GCTGGATCGTTTTTGAACTGGGCGATGGCTGGAACCCTGTCCGCGGTGCTGATGGTCATCGTGATGATCACGTTCTTCAT
CATTAAACTTGCTACGCGACTGTTTGTCAGGGGACGGGCCGATGCCGCATTCAGTTAG

Upstream 100 bases:

>100_bases
CGGACGAAGAGACTATGTTCGACACCCACCAGGAGGTTCACCTCAGCATTCGCGAGGCGGATTGCCGCCCCGTACCGCGG
GACGCAAAGGAGCCGCGCGG

Downstream 100 bases:

>100_bases
CATACAGAAGGCGTCGGGGTGGCTGGGCAACGGGCTGCTGCGGATCTTCTCCTGGATCGTGTTTCTCTTCCTCGTGTCGC
CGCTTCTTGTGGTTGTGCCG

Product: binding-protein-dependent transport systems inner membrane component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MNQGFQAIKAQAGSLALVSPALLLLGILFFYPILLLFLTTLELENIGNYAQVVSSDTSMQVFFNTLRIAVIVTVVDLVLA
YPLAVYISRLSPTAKTFAFFLVLIPLWTSQLVRTYAWMVLLGRNGPINNALVWLGIVDAPIRLANSEFAVIAGMTHILLP
YMLLPIYNSVSKIDPSLFDAARGLGAGAFSTFTRVLMPLSIQGVLAGVSLVLVLSLGVFIMPALLGGGRVPVIALLIEQQ
AGSFLNWAMAGTLSAVLMVIVMITFFIIKLATRLFVRGRADAAFS

Sequences:

>Translated_285_residues
MNQGFQAIKAQAGSLALVSPALLLLGILFFYPILLLFLTTLELENIGNYAQVVSSDTSMQVFFNTLRIAVIVTVVDLVLA
YPLAVYISRLSPTAKTFAFFLVLIPLWTSQLVRTYAWMVLLGRNGPINNALVWLGIVDAPIRLANSEFAVIAGMTHILLP
YMLLPIYNSVSKIDPSLFDAARGLGAGAFSTFTRVLMPLSIQGVLAGVSLVLVLSLGVFIMPALLGGGRVPVIALLIEQQ
AGSFLNWAMAGTLSAVLMVIVMITFFIIKLATRLFVRGRADAAFS
>Mature_285_residues
MNQGFQAIKAQAGSLALVSPALLLLGILFFYPILLLFLTTLELENIGNYAQVVSSDTSMQVFFNTLRIAVIVTVVDLVLA
YPLAVYISRLSPTAKTFAFFLVLIPLWTSQLVRTYAWMVLLGRNGPINNALVWLGIVDAPIRLANSEFAVIAGMTHILLP
YMLLPIYNSVSKIDPSLFDAARGLGAGAFSTFTRVLMPLSIQGVLAGVSLVLVLSLGVFIMPALLGGGRVPVIALLIEQQ
AGSFLNWAMAGTLSAVLMVIVMITFFIIKLATRLFVRGRADAAFS

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=230, Percent_Identity=33.9130434782609, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1787080, Length=224, Percent_Identity=36.1607142857143, Blast_Score=154, Evalue=7e-39,
Organism=Escherichia coli, GI1787713, Length=243, Percent_Identity=30.8641975308642, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30754; Mature: 30754

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQGFQAIKAQAGSLALVSPALLLLGILFFYPILLLFLTTLELENIGNYAQVVSSDTSMQ
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHH
VFFNTLRIAVIVTVVDLVLAYPLAVYISRLSPTAKTFAFFLVLIPLWTSQLVRTYAWMVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGRNGPINNALVWLGIVDAPIRLANSEFAVIAGMTHILLPYMLLPIYNSVSKIDPSLFDA
CCCCCCCCCCEEEEEECCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
ARGLGAGAFSTFTRVLMPLSIQGVLAGVSLVLVLSLGVFIMPALLGGGRVPVIALLIEQQ
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
AGSFLNWAMAGTLSAVLMVIVMITFFIIKLATRLFVRGRADAAFS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNQGFQAIKAQAGSLALVSPALLLLGILFFYPILLLFLTTLELENIGNYAQVVSSDTSMQ
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHH
VFFNTLRIAVIVTVVDLVLAYPLAVYISRLSPTAKTFAFFLVLIPLWTSQLVRTYAWMVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGRNGPINNALVWLGIVDAPIRLANSEFAVIAGMTHILLPYMLLPIYNSVSKIDPSLFDA
CCCCCCCCCCEEEEEECCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
ARGLGAGAFSTFTRVLMPLSIQGVLAGVSLVLVLSLGVFIMPALLGGGRVPVIALLIEQQ
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
AGSFLNWAMAGTLSAVLMVIVMITFFIIKLATRLFVRGRADAAFS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]