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The map label for this gene is gatB

Identifier: 110634072

GI number: 110634072

Start: 1843040

End: 1844539

Strand: Reverse

Name: gatB

Synonym: Meso_1720

Alternate gene names: 110634072

Gene position: 1844539-1843040 (Counterclockwise)

Preceding gene: 110634074

Following gene: 110634071

Centisome position: 41.8

GC content: 61.0

Gene sequence:

>1500_bases
ATGACAATCATCGATACGCGCACGCCCGATCCAAAGCGGCTCATTCCCGGCGCCACCGGAGACTGGGAAGTGATCATCGG
CATGGAAATCCATGCCCAGGTGACGTCTCGCGCGAAGCTCTTTTCCGGCGCCTCTACTGAGTTCGGCGCCGAGCCAAACG
CCAATGTTTCGCTGGTGGACGCGGCCATGCCCGGCATGCTCCCCGTCATCAACGAGGAATGCGTGAAGCAGGCGATCCGC
ACCGGGCTTGGGCTGAAGGCGCAGATCAATCATCGCTCCGTTTTCGACCGGAAGAACTATTTTTATCCGGACCTGCCGCA
GGGCTATCAGATCTCGCAATACAAGCAGCCGATCGTGGGTGAGGGGATCGTAATCGTCTCCGTCGGCCCGGCCAAGGACG
GCAGCTTTGAGGAGATCCAGGTCGGCATCGAGCGCCTGCATCTGGAGCAGGATGCGGGCAAGTCGCTGCACGACCAGCAC
CCGACCATGTCCTATGTGGACCTGAACCGCTCCGGCGTGGCGCTGATGGAGATCGTGTCCAAGCCGGACATGCGCTCCGC
CGATGAGGCGAAGGCCTTTCTCACCAAGCTGCGCAGCATCCTGCGGTATCTGGGGACCTGCGACGGCAATATGGACGAAG
GCTCCATGCGTGCGGACGTGAACGTGTCGGTGCGCCGGCCGGGCGAGCCCTTCGGCACGCGTTGCGAGATCAAGAACGTA
AATTCGATCCGCTTTGTCGGCCAGGCGATCGAATCGGAGGCGCGGCGGCAGATCGCCATTCTCGAGGACGGCGGCGCGAT
TGACCAGGAGACGCGGCTTTTCGATCCCGGCAAAGGCGAAACGCGTTCGATGCGCTCGAAGGAAGAGGCGCACGATTACC
GCTACTTCCCCGACCCGGACCTTGTGCCGCTCGTTTTCGACCAGGCCTATGTGGATGCACTTGCCGCCGAGCTGCCGGAG
CTTCCGGACGAGAAGAAGGCGCGCTTCATGGCGAGTTTCGGCCTGTCCGAATACGATGCTTCTGTACTCGTCGCGGAGAG
GGCGACCGCCGCGTATTTCGAGAAGGTCGCGGCCGGCCGCGACGGCAAAGCGGCGGCGAACTGGGTGATCAACGATCTTT
TAGGTGCCTTGAACAGGACAGGAAAAGATATTGAAGATACACCGGTTTCTCCCGATCATCTTGGCACGATCATCGACCTG
ATCAAGGACGGCACGATCTCTGGAAAGATCGCCAAGGACCTGTTCGAGATCGTCTGGAACGAGGGCGGCGACCCGGTAAG
GATAGTGGAGGAGCGGGGCCTGAAACAGGTGACCGACACCGGTGCCATTGAGAAGGCGGTGGACGAGGTAATTGCTGCCA
ATCCGGACAAGGTGGAACAGGCGAAGGCTAAGCCAACGCTCGCTGGCTGGTTTGTCGGCCAGGTGATGAAGGCGACCGGC
GGCAAGGCCAACCCGCAGGCCGTCAACGCGCTGGTGAATCAAAAACTAGGGATCGAATGA

Upstream 100 bases:

>100_bases
GCCTTGTCAGGCTGCCGCGATTGTTTTAGCGAACACGGCTGTAGTTACGTCGTAGCGAAACCCGCTACATGTTTCTCCCA
GAATCCGACTGCATGGACCC

Downstream 100 bases:

>100_bases
CAGGAGCGGGGCGCACGCGTAAAGGCCAAAGCGCGTGAGGCCCTTGCGTTCGGGCCGCCGCGCCGCCATAAGCAGCGCAA
AAGGTCTGTCACTGCCGCAC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MTIIDTRTPDPKRLIPGATGDWEVIIGMEIHAQVTSRAKLFSGASTEFGAEPNANVSLVDAAMPGMLPVINEECVKQAIR
TGLGLKAQINHRSVFDRKNYFYPDLPQGYQISQYKQPIVGEGIVIVSVGPAKDGSFEEIQVGIERLHLEQDAGKSLHDQH
PTMSYVDLNRSGVALMEIVSKPDMRSADEAKAFLTKLRSILRYLGTCDGNMDEGSMRADVNVSVRRPGEPFGTRCEIKNV
NSIRFVGQAIESEARRQIAILEDGGAIDQETRLFDPGKGETRSMRSKEEAHDYRYFPDPDLVPLVFDQAYVDALAAELPE
LPDEKKARFMASFGLSEYDASVLVAERATAAYFEKVAAGRDGKAAANWVINDLLGALNRTGKDIEDTPVSPDHLGTIIDL
IKDGTISGKIAKDLFEIVWNEGGDPVRIVEERGLKQVTDTGAIEKAVDEVIAANPDKVEQAKAKPTLAGWFVGQVMKATG
GKANPQAVNALVNQKLGIE

Sequences:

>Translated_499_residues
MTIIDTRTPDPKRLIPGATGDWEVIIGMEIHAQVTSRAKLFSGASTEFGAEPNANVSLVDAAMPGMLPVINEECVKQAIR
TGLGLKAQINHRSVFDRKNYFYPDLPQGYQISQYKQPIVGEGIVIVSVGPAKDGSFEEIQVGIERLHLEQDAGKSLHDQH
PTMSYVDLNRSGVALMEIVSKPDMRSADEAKAFLTKLRSILRYLGTCDGNMDEGSMRADVNVSVRRPGEPFGTRCEIKNV
NSIRFVGQAIESEARRQIAILEDGGAIDQETRLFDPGKGETRSMRSKEEAHDYRYFPDPDLVPLVFDQAYVDALAAELPE
LPDEKKARFMASFGLSEYDASVLVAERATAAYFEKVAAGRDGKAAANWVINDLLGALNRTGKDIEDTPVSPDHLGTIIDL
IKDGTISGKIAKDLFEIVWNEGGDPVRIVEERGLKQVTDTGAIEKAVDEVIAANPDKVEQAKAKPTLAGWFVGQVMKATG
GKANPQAVNALVNQKLGIE
>Mature_498_residues
TIIDTRTPDPKRLIPGATGDWEVIIGMEIHAQVTSRAKLFSGASTEFGAEPNANVSLVDAAMPGMLPVINEECVKQAIRT
GLGLKAQINHRSVFDRKNYFYPDLPQGYQISQYKQPIVGEGIVIVSVGPAKDGSFEEIQVGIERLHLEQDAGKSLHDQHP
TMSYVDLNRSGVALMEIVSKPDMRSADEAKAFLTKLRSILRYLGTCDGNMDEGSMRADVNVSVRRPGEPFGTRCEIKNVN
SIRFVGQAIESEARRQIAILEDGGAIDQETRLFDPGKGETRSMRSKEEAHDYRYFPDPDLVPLVFDQAYVDALAAELPEL
PDEKKARFMASFGLSEYDASVLVAERATAAYFEKVAAGRDGKAAANWVINDLLGALNRTGKDIEDTPVSPDHLGTIIDLI
KDGTISGKIAKDLFEIVWNEGGDPVRIVEERGLKQVTDTGAIEKAVDEVIAANPDKVEQAKAKPTLAGWFVGQVMKATGG
KANPQAVNALVNQKLGIE

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=495, Percent_Identity=41.2121212121212, Blast_Score=377, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI115534094, Length=298, Percent_Identity=41.6107382550336, Blast_Score=214, Evalue=8e-56,
Organism=Saccharomyces cerevisiae, GI6319391, Length=510, Percent_Identity=30.9803921568627, Blast_Score=215, Evalue=1e-56,
Organism=Drosophila melanogaster, GI45550808, Length=485, Percent_Identity=37.7319587628866, Blast_Score=334, Evalue=8e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_MESSB (Q11HL0)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_674280.1
- ProteinModelPortal:   Q11HL0
- SMR:   Q11HL0
- STRING:   Q11HL0
- GeneID:   4181350
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_1720
- NMPDR:   fig|266779.1.peg.3803
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- PhylomeDB:   Q11HL0
- ProtClustDB:   PRK05477
- BioCyc:   MSP266779:MESO_1720-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 54420; Mature: 54289

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIIDTRTPDPKRLIPGATGDWEVIIGMEIHAQVTSRAKLFSGASTEFGAEPNANVSLVD
CCEECCCCCCCCCCCCCCCCCEEEEEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
AAMPGMLPVINEECVKQAIRTGLGLKAQINHRSVFDRKNYFYPDLPQGYQISQYKQPIVG
CCCCCCCHHHHHHHHHHHHHHCCCCEEEECCHHHHCCCCCCCCCCCCCCCHHHHCCCCCC
EGIVIVSVGPAKDGSFEEIQVGIERLHLEQDAGKSLHDQHPTMSYVDLNRSGVALMEIVS
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEECCCCCHHHHHHHC
KPDMRSADEAKAFLTKLRSILRYLGTCDGNMDEGSMRADVNVSVRRPGEPFGTRCEIKNV
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCEEEECCC
NSIRFVGQAIESEARRQIAILEDGGAIDQETRLFDPGKGETRSMRSKEEAHDYRYFPDPD
CHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
LVPLVFDQAYVDALAAELPELPDEKKARFMASFGLSEYDASVLVAERATAAYFEKVAAGR
CHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHCCC
DGKAAANWVINDLLGALNRTGKDIEDTPVSPDHLGTIIDLIKDGTISGKIAKDLFEIVWN
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
EGGDPVRIVEERGLKQVTDTGAIEKAVDEVIAANPDKVEQAKAKPTLAGWFVGQVMKATG
CCCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHCC
GKANPQAVNALVNQKLGIE
CCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TIIDTRTPDPKRLIPGATGDWEVIIGMEIHAQVTSRAKLFSGASTEFGAEPNANVSLVD
CEECCCCCCCCCCCCCCCCCEEEEEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
AAMPGMLPVINEECVKQAIRTGLGLKAQINHRSVFDRKNYFYPDLPQGYQISQYKQPIVG
CCCCCCCHHHHHHHHHHHHHHCCCCEEEECCHHHHCCCCCCCCCCCCCCCHHHHCCCCCC
EGIVIVSVGPAKDGSFEEIQVGIERLHLEQDAGKSLHDQHPTMSYVDLNRSGVALMEIVS
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEECCCCCHHHHHHHC
KPDMRSADEAKAFLTKLRSILRYLGTCDGNMDEGSMRADVNVSVRRPGEPFGTRCEIKNV
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCEEEECCC
NSIRFVGQAIESEARRQIAILEDGGAIDQETRLFDPGKGETRSMRSKEEAHDYRYFPDPD
CHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
LVPLVFDQAYVDALAAELPELPDEKKARFMASFGLSEYDASVLVAERATAAYFEKVAAGR
CHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHCCC
DGKAAANWVINDLLGALNRTGKDIEDTPVSPDHLGTIIDLIKDGTISGKIAKDLFEIVWN
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
EGGDPVRIVEERGLKQVTDTGAIEKAVDEVIAANPDKVEQAKAKPTLAGWFVGQVMKATG
CCCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHCC
GKANPQAVNALVNQKLGIE
CCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA