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The map label for this gene is lipA

Identifier: 110633976

GI number: 110633976

Start: 1749490

End: 1750458

Strand: Reverse

Name: lipA

Synonym: Meso_1624

Alternate gene names: 110633976

Gene position: 1750458-1749490 (Counterclockwise)

Preceding gene: 110633977

Following gene: 110633975

Centisome position: 39.67

GC content: 60.47

Gene sequence:

>969_bases
ATGGTCACCGTCCTCGACACCGTCTCCAAGCCGCGCCCGCGCCATCCCGAGAAGGCGCATCGGCCCGATCAGGAAGTTCT
GCGCAAGCCGGACTGGATCCGTGTCAAAGCGCCTACCTCCAGGGGCTATTTGGAAACGCGCGAGATCGTGAAGACCAACA
AGCTTGTCACGGTCTGCGAGGAGGCGGGCTGTCCCAATATCGGCGAATGCTGGGACAAGAAGCACGCGACCTTCATGATC
ATGGGTGAGATCTGCACCCGTGCCTGCGCCTTCTGTAATGTCGCGACGGGCATTCCCGGCCCGCTCGATCCGAACGAGCC
CGCCAACGTGGCGAAAGCTGTCCGCGAGATGGGGCTGAACCACGTCGTCATCACCTCCGTCGACCGGGACGATCTGAATG
ACGGGGGCGCGCAGCATTTTGCCGACGTGATCCAAGCGATCCGTGCGGCGACGCCGCAGACCACCATCGAGATCCTGACG
CCGGACTTCCTGCGCAAGGACGGCGCTTTGGAGTTGGTCGTGGCCGCCAAACCAGATGTCTTCAACCACAATCTGGAGAC
AGTCCCCTCGAAATACCTGACGGTGCGCCCTGGCGCCCGCTACTTCCATTCCATCCGGCTCTTACAGAGGGTGAAGGAGA
TCGATCCTTCGATCTTTACGAAATCCGGAATTATGGTCGGGCTTGGCGAGGAGCGAAACGAGGTGCTTCAGCTGATGGAT
GACCTGCGCTCGGCGGATGTCGATTTCATCACAATCGGGCAGTATCTCCAGCCCACTAAAAAGCACCACCCAGTGAAGAA
GTTCGTGACGCCGGAAGAGTTCAAATCCTACGAGACCATCGCCTACACCAAGGGCTTTCTAATGGCTTCCTCCAGCCCGC
TCACGCGATCTTCGCACCATGCGGGCGAGGATTTCGAGCGCTTGCGCGCCGCGCGCGAGGCACGGCTCTCCCTGGCGAAG
ACGGCTTAA

Upstream 100 bases:

>100_bases
CACTGCCGTTAAAAAATGCGCCCGCCTGCGGAACAGCAGGCGGGTCTTTCGTATCCGTCGGAAACGCTTATATGAGACCG
GCGAACAGAAAGTAGACAAT

Downstream 100 bases:

>100_bases
GACGCTTAATGCCCAAGCACGAGACGGTTCGAAGGGTCAGTCATCCACCCGATCAGATGTTCGCCCTGGTGGCGGATGTG
GAAAGCTATCCCGAATTCCT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MVTVLDTVSKPRPRHPEKAHRPDQEVLRKPDWIRVKAPTSRGYLETREIVKTNKLVTVCEEAGCPNIGECWDKKHATFMI
MGEICTRACAFCNVATGIPGPLDPNEPANVAKAVREMGLNHVVITSVDRDDLNDGGAQHFADVIQAIRAATPQTTIEILT
PDFLRKDGALELVVAAKPDVFNHNLETVPSKYLTVRPGARYFHSIRLLQRVKEIDPSIFTKSGIMVGLGEERNEVLQLMD
DLRSADVDFITIGQYLQPTKKHHPVKKFVTPEEFKSYETIAYTKGFLMASSSPLTRSSHHAGEDFERLRAAREARLSLAK
TA

Sequences:

>Translated_322_residues
MVTVLDTVSKPRPRHPEKAHRPDQEVLRKPDWIRVKAPTSRGYLETREIVKTNKLVTVCEEAGCPNIGECWDKKHATFMI
MGEICTRACAFCNVATGIPGPLDPNEPANVAKAVREMGLNHVVITSVDRDDLNDGGAQHFADVIQAIRAATPQTTIEILT
PDFLRKDGALELVVAAKPDVFNHNLETVPSKYLTVRPGARYFHSIRLLQRVKEIDPSIFTKSGIMVGLGEERNEVLQLMD
DLRSADVDFITIGQYLQPTKKHHPVKKFVTPEEFKSYETIAYTKGFLMASSSPLTRSSHHAGEDFERLRAAREARLSLAK
TA
>Mature_322_residues
MVTVLDTVSKPRPRHPEKAHRPDQEVLRKPDWIRVKAPTSRGYLETREIVKTNKLVTVCEEAGCPNIGECWDKKHATFMI
MGEICTRACAFCNVATGIPGPLDPNEPANVAKAVREMGLNHVVITSVDRDDLNDGGAQHFADVIQAIRAATPQTTIEILT
PDFLRKDGALELVVAAKPDVFNHNLETVPSKYLTVRPGARYFHSIRLLQRVKEIDPSIFTKSGIMVGLGEERNEVLQLMD
DLRSADVDFITIGQYLQPTKKHHPVKKFVTPEEFKSYETIAYTKGFLMASSSPLTRSSHHAGEDFERLRAAREARLSLAK
TA

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=303, Percent_Identity=44.8844884488449, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI37577164, Length=252, Percent_Identity=45.6349206349206, Blast_Score=231, Evalue=1e-60,
Organism=Escherichia coli, GI1786846, Length=280, Percent_Identity=52.1428571428571, Blast_Score=304, Evalue=5e-84,
Organism=Caenorhabditis elegans, GI32564533, Length=256, Percent_Identity=46.484375, Blast_Score=244, Evalue=4e-65,
Organism=Saccharomyces cerevisiae, GI6324770, Length=296, Percent_Identity=43.9189189189189, Blast_Score=254, Evalue=2e-68,
Organism=Drosophila melanogaster, GI221513272, Length=295, Percent_Identity=43.3898305084746, Blast_Score=256, Evalue=2e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_MESSB (Q11HV6)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_674184.1
- ProteinModelPortal:   Q11HV6
- STRING:   Q11HV6
- GeneID:   4179830
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_1624
- NMPDR:   fig|266779.1.peg.370
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   DPHEPQR
- PhylomeDB:   Q11HV6
- ProtClustDB:   PRK05481
- BioCyc:   MSP266779:MESO_1624-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 35964; Mature: 35964

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTVLDTVSKPRPRHPEKAHRPDQEVLRKPDWIRVKAPTSRGYLETREIVKTNKLVTVCE
CEEEECCCCCCCCCCCHHHCCCCHHHHCCCCEEEEECCCCCCCHHHHHHHHHCHHHHHHH
EAGCPNIGECWDKKHATFMIMGEICTRACAFCNVATGIPGPLDPNEPANVAKAVREMGLN
HCCCCCHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCC
HVVITSVDRDDLNDGGAQHFADVIQAIRAATPQTTIEILTPDFLRKDGALELVVAAKPDV
EEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHHCCCCCEEEEEECCCCH
FNHNLETVPSKYLTVRPGARYFHSIRLLQRVKEIDPSIFTKSGIMVGLGEERNEVLQLMD
HCCCHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCHHHEECCCEEEECCCCHHHHHHHHH
DLRSADVDFITIGQYLQPTKKHHPVKKFVTPEEFKSYETIAYTKGFLMASSSPLTRSSHH
HHHHCCCCEEECCHHHCCHHHCCCHHHCCCHHHHHCCHHHHHHCCEEEECCCCCCCCCCC
AGEDFERLRAAREARLSLAKTA
CCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVTVLDTVSKPRPRHPEKAHRPDQEVLRKPDWIRVKAPTSRGYLETREIVKTNKLVTVCE
CEEEECCCCCCCCCCCHHHCCCCHHHHCCCCEEEEECCCCCCCHHHHHHHHHCHHHHHHH
EAGCPNIGECWDKKHATFMIMGEICTRACAFCNVATGIPGPLDPNEPANVAKAVREMGLN
HCCCCCHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCC
HVVITSVDRDDLNDGGAQHFADVIQAIRAATPQTTIEILTPDFLRKDGALELVVAAKPDV
EEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHHCCCCCEEEEEECCCCH
FNHNLETVPSKYLTVRPGARYFHSIRLLQRVKEIDPSIFTKSGIMVGLGEERNEVLQLMD
HCCCHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCHHHEECCCEEEECCCCHHHHHHHHH
DLRSADVDFITIGQYLQPTKKHHPVKKFVTPEEFKSYETIAYTKGFLMASSSPLTRSSHH
HHHHCCCCEEECCHHHCCHHHCCCHHHCCCHHHHHCCHHHHHHCCEEEECCCCCCCCCCC
AGEDFERLRAAREARLSLAKTA
CCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA