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The map label for this gene is gltX1

Identifier: 110633888

GI number: 110633888

Start: 1651195

End: 1652565

Strand: Direct

Name: gltX1

Synonym: Meso_1535

Alternate gene names: 110633888

Gene position: 1651195-1652565 (Clockwise)

Preceding gene: 110633887

Following gene: 110633891

Centisome position: 37.42

GC content: 60.61

Gene sequence:

>1371_bases
ATGACTGTAACAGTTCGCTTCGCGCCGTCACCGACGGGGCAGATCCATATCGGCAACGCACGCACGGCGCTCTTCAACTG
GCTGTTCGCCAGAAGGGCAGGGGGAAGCTTCGTGCTGCGCTTCGACGACACCGATGTCGAGCGCTCACGCAGGGAATACG
CCGATCAGATAGAGATCGATCTCGCATGGCTAGGCATCAAGCCGGACCTGACGGTTCGCCAGTCCGAACGGTTTGGTCTC
TACAATCAGGCGGTGGAAAAGCTGAAGGAAGCGGGGCGTCTTTACCCCTGCTATGAGACGGCGGAGGAGCTGGAGCTTCG
CCGCAAGATCAGGCTCTCGCGCCGGCTGCCGCCGATCTATGGCCGTGAGGCGTTGAAGCTCACCGAAGAGCAGAAGGCGG
CCTATGAGGCGGAAGGCAGGAAGCCGCATTGGCGCTTCCTTCTCCCCAATTTCGACAGCGATCCTTTTTCGACCGGGCGC
ACCGAGATCCATTGGGATGATGTCATCCGTGGCCCGCAGACGGTCGATCTGGCCTCGATGTCGGATCCGGTCCTCGTCAG
GGAGGACGGGACATTTCTCTACACGTTGCCGTCGGTGGTGGACGATATCGATCTCGGTATCACCCACGTGATCCGCGGTG
ACGACCACGTGACCAACACCGGCGCACAGATCGCGCTTTTTGAGGCTTTGGGCGCGAAGGCGCCGTGCTTCGGTCATCAT
AACCTGTTGACCACCGTCACCGGCGAGGGGCTTTCCAAGCGCACTGGCGCTCTCTCGATTGCAAGCCTGAGGAAGGCCGG
TATAGAACCGATGGCCGTTGCATCGCTCGCGGTGCTTATCGGCACGTCGGAGGCCGTGACAGCCTATCGCGACATGGAAG
AGCTTGCGGCCCATTTCGAACCGGCATCCAGCTCCAAATCCTCAGCAAAATTCGATCCGGCGGAGCTGGAGGCTCTGAGC
CGATCCCTCATTCAGGCAATGCCCTTCGAAGCAGCCGCTCCGCGCCTGGCGGCAATGGGGGTCGAAGGATCGATAGCCGA
GCCGTTCTGGAATGCGGTGCGCGGCAATATCGATAAGCTCTCCGAGGCAGCCGAATGGTGGCATATCGTCATGAAGGGCC
CGGAACCGACGGAGTTCACCGAGGACGACCGCAATTTCGTGAATGAGGCTTTCGACCTACTCCCACCAGAGCCATGGGAC
CGCGAGACGTGGCGCAGTTGGACGGAAAAGGTGAAAGAGAAAACAGGACGCAAGGGCAAAGCACTTTTCAGGCCCTTGCG
CCTCGCACTCACCGGGCGCGAGTCCGGGCCGGAGCTCGCAGATTTATTGCCTCTTCTGGGTCGGGAAAGAACTTTGGCCC
GACAACCCTGA

Upstream 100 bases:

>100_bases
GGCTTTACTGCCTGGGGGCCGCGCTCCTTATGCTCATTGTCTCGCGGCGGCTCCAGATGCGATCCTTGCAAATGGTCGAG
GCGTCGGCTACCTCCGCGCC

Downstream 100 bases:

>100_bases
CATTCTTCTCCGCTTCCGTCATGGGCCGAGCGGAAGAGTCCCTGGAAAAAATCCGCTGTTCCACAGGAACAGCGCTCGCA
TCCGGGATCTCCTGGAAGGA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MTVTVRFAPSPTGQIHIGNARTALFNWLFARRAGGSFVLRFDDTDVERSRREYADQIEIDLAWLGIKPDLTVRQSERFGL
YNQAVEKLKEAGRLYPCYETAEELELRRKIRLSRRLPPIYGREALKLTEEQKAAYEAEGRKPHWRFLLPNFDSDPFSTGR
TEIHWDDVIRGPQTVDLASMSDPVLVREDGTFLYTLPSVVDDIDLGITHVIRGDDHVTNTGAQIALFEALGAKAPCFGHH
NLLTTVTGEGLSKRTGALSIASLRKAGIEPMAVASLAVLIGTSEAVTAYRDMEELAAHFEPASSSKSSAKFDPAELEALS
RSLIQAMPFEAAAPRLAAMGVEGSIAEPFWNAVRGNIDKLSEAAEWWHIVMKGPEPTEFTEDDRNFVNEAFDLLPPEPWD
RETWRSWTEKVKEKTGRKGKALFRPLRLALTGRESGPELADLLPLLGRERTLARQP

Sequences:

>Translated_456_residues
MTVTVRFAPSPTGQIHIGNARTALFNWLFARRAGGSFVLRFDDTDVERSRREYADQIEIDLAWLGIKPDLTVRQSERFGL
YNQAVEKLKEAGRLYPCYETAEELELRRKIRLSRRLPPIYGREALKLTEEQKAAYEAEGRKPHWRFLLPNFDSDPFSTGR
TEIHWDDVIRGPQTVDLASMSDPVLVREDGTFLYTLPSVVDDIDLGITHVIRGDDHVTNTGAQIALFEALGAKAPCFGHH
NLLTTVTGEGLSKRTGALSIASLRKAGIEPMAVASLAVLIGTSEAVTAYRDMEELAAHFEPASSSKSSAKFDPAELEALS
RSLIQAMPFEAAAPRLAAMGVEGSIAEPFWNAVRGNIDKLSEAAEWWHIVMKGPEPTEFTEDDRNFVNEAFDLLPPEPWD
RETWRSWTEKVKEKTGRKGKALFRPLRLALTGRESGPELADLLPLLGRERTLARQP
>Mature_455_residues
TVTVRFAPSPTGQIHIGNARTALFNWLFARRAGGSFVLRFDDTDVERSRREYADQIEIDLAWLGIKPDLTVRQSERFGLY
NQAVEKLKEAGRLYPCYETAEELELRRKIRLSRRLPPIYGREALKLTEEQKAAYEAEGRKPHWRFLLPNFDSDPFSTGRT
EIHWDDVIRGPQTVDLASMSDPVLVREDGTFLYTLPSVVDDIDLGITHVIRGDDHVTNTGAQIALFEALGAKAPCFGHHN
LLTTVTGEGLSKRTGALSIASLRKAGIEPMAVASLAVLIGTSEAVTAYRDMEELAAHFEPASSSKSSAKFDPAELEALSR
SLIQAMPFEAAAPRLAAMGVEGSIAEPFWNAVRGNIDKLSEAAEWWHIVMKGPEPTEFTEDDRNFVNEAFDLLPPEPWDR
ETWRSWTEKVKEKTGRKGKALFRPLRLALTGRESGPELADLLPLLGRERTLARQP

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=492, Percent_Identity=29.8780487804878, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI62241042, Length=257, Percent_Identity=28.7937743190661, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI4826960, Length=308, Percent_Identity=27.2727272727273, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1788743, Length=337, Percent_Identity=37.0919881305638, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1786895, Length=145, Percent_Identity=33.7931034482759, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71993394, Length=477, Percent_Identity=28.3018867924528, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17543332, Length=103, Percent_Identity=37.8640776699029, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17511007, Length=269, Percent_Identity=24.907063197026, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6324540, Length=490, Percent_Identity=28.7755102040816, Blast_Score=177, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6324742, Length=109, Percent_Identity=36.697247706422, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6321192, Length=102, Percent_Identity=40.1960784313725, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24665321, Length=489, Percent_Identity=28.6298568507157, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24649466, Length=110, Percent_Identity=34.5454545454545, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17864488, Length=138, Percent_Identity=35.5072463768116, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE1_MESSB (Q11I44)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_674096.1
- ProteinModelPortal:   Q11I44
- SMR:   Q11I44
- STRING:   Q11I44
- GeneID:   4180580
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_1535
- NMPDR:   fig|266779.1.peg.282
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   PHWRFKL
- ProtClustDB:   PRK12558
- BioCyc:   MSP266779:MESO_1535-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 50979; Mature: 50848

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 253-253

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTVRFAPSPTGQIHIGNARTALFNWLFARRAGGSFVLRFDDTDVERSRREYADQIEID
CEEEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHEEEE
LAWLGIKPDLTVRQSERFGLYNQAVEKLKEAGRLYPCYETAEELELRRKIRLSRRLPPIY
EEEEECCCCCEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
GREALKLTEEQKAAYEAEGRKPHWRFLLPNFDSDPFSTGRTEIHWDDVIRGPQTVDLASM
CCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCEEEHHHHCCCCCCEEECCC
SDPVLVREDGTFLYTLPSVVDDIDLGITHVIRGDDHVTNTGAQIALFEALGAKAPCFGHH
CCCEEEEECCCEEEECCHHHHHHHCCEEEEECCCCCCCCCCCCEEEEHHHCCCCCCCCCC
NLLTTVTGEGLSKRTGALSIASLRKAGIEPMAVASLAVLIGTSEAVTAYRDMEELAAHFE
CEEEEECCCCCCHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
PASSSKSSAKFDPAELEALSRSLIQAMPFEAAAPRLAAMGVEGSIAEPFWNAVRGNIDKL
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCHHHHCCCCCCCHHHHHHHHCCCHHHH
SEAAEWWHIVMKGPEPTEFTEDDRNFVNEAFDLLPPEPWDRETWRSWTEKVKEKTGRKGK
HHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCH
ALFRPLRLALTGRESGPELADLLPLLGRERTLARQP
HHHHHHHHEEECCCCCCHHHHHHHHHCCCCHHCCCC
>Mature Secondary Structure 
TVTVRFAPSPTGQIHIGNARTALFNWLFARRAGGSFVLRFDDTDVERSRREYADQIEID
EEEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHEEEE
LAWLGIKPDLTVRQSERFGLYNQAVEKLKEAGRLYPCYETAEELELRRKIRLSRRLPPIY
EEEEECCCCCEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
GREALKLTEEQKAAYEAEGRKPHWRFLLPNFDSDPFSTGRTEIHWDDVIRGPQTVDLASM
CCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCEEEHHHHCCCCCCEEECCC
SDPVLVREDGTFLYTLPSVVDDIDLGITHVIRGDDHVTNTGAQIALFEALGAKAPCFGHH
CCCEEEEECCCEEEECCHHHHHHHCCEEEEECCCCCCCCCCCCEEEEHHHCCCCCCCCCC
NLLTTVTGEGLSKRTGALSIASLRKAGIEPMAVASLAVLIGTSEAVTAYRDMEELAAHFE
CEEEEECCCCCCHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
PASSSKSSAKFDPAELEALSRSLIQAMPFEAAAPRLAAMGVEGSIAEPFWNAVRGNIDKL
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCHHHHCCCCCCCHHHHHHHHCCCHHHH
SEAAEWWHIVMKGPEPTEFTEDDRNFVNEAFDLLPPEPWDRETWRSWTEKVKEKTGRKGK
HHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCH
ALFRPLRLALTGRESGPELADLLPLLGRERTLARQP
HHHHHHHHEEECCCCCCHHHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA