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The map label for this gene is otsA [H]

Identifier: 110633880

GI number: 110633880

Start: 1643920

End: 1645311

Strand: Reverse

Name: otsA [H]

Synonym: Meso_1527

Alternate gene names: 110633880

Gene position: 1645311-1643920 (Counterclockwise)

Preceding gene: 110633881

Following gene: 110633878

Centisome position: 37.29

GC content: 59.41

Gene sequence:

>1392_bases
GTGAAAAGGCTTGTCGTCGTCTCGAACCGCGTAGCGGACCTGACAAGGACGTCCCAGGCCGGCGGGCTTGCGGTCGGCCT
TGCGGATTCGCTGCGTCAGCGCGGCGGCGTATGGATGGGCTGGGACGGGCAGACGCTGAAATACGAGGAGGAGCCGGAGC
CGCGCATCCGCAAGATTGGCGAGGTGACCCAGGCGACCATTCCGCTCACCGAGCAGGACTATGCGGAATACTATCTGGGC
TTTGCCAACAGCGTTCTATGGCCGCTCTTTCACTACAGGCTCGATCTGGTGGAGTACAAAACGGCCTTCCTGGAAGGCTA
TCGGCGCGTGAATGCGAAATTCGCAAATGCGCTGGTGTCGATTCTCCGTGACGACGATCTAATCTGGATCCATGATTACC
ATCTTATTCCGCTCGCGGCGGAGTTGCGCGAGCGCGGCTGCAAGCAGCGTATCGGTTTTTTCCTGCACATCCCCTTCCCG
CCCCCGGAAATTCTCGCCGCTGTTCCCCGCCACCGTTGGCTGGTGGAGTGCCTGCTGAAGCATGATGTGATCGGTTTTCA
GACGCATACGGATCTCAACAACCTTCTGCGCTATGTCGAGGATCATCTGGGTTGTGAAATTCTGGCCGACGGGCGCATCA
GGACGCGCGAGCGCGAGGTGATCGCCGGTTTCTATCCCATCGGCATCGATGTGGATGCCTTTGTGGGCATGGCCGAGAAG
CCGAATGACGAGGTGCAAATCGAAACCAAACGGCGCGAGATCCTTGGACGAAGGCAGATTATCGGCGTCGACAGGCTGGA
CTACACGAAAGGCTTGCCGGATCGATTGCGCGCCTTTGCCCGCCTTCTGGAGCTCTATCCGGAGATGAGGAAATCAGTCG
CGCTGATGCAAATCGCCACGCCCACACGCGAAGAGGTCGAGGCCTATGTGGATATCCGCAAGGAATTGGAGGCTCTGTCC
GGCGCCATCAACGGCCGCTTCGCCGATTTTAACTGGACGCCTGTGCGTTACATCCACGGCACCGTGCCGCGTGACGCGCT
GGCAGCTCTCTACCGGGCGAGCCAGGTGGGCTTTGTCACGCCGCTTCGTGACGGGATGAACCTCGTCGCCAAGGAATATG
TCGCCGCGCAATCCATGGATGATCCGGGCGTGCTCGTTCTATCAAAGTTTGCAGGGGCAGCGGAGGATCTGCACGAGGCG
CTGATCGTCAACCCGTATGACGTGGACGACATGGCTCGCAATCTTTGCCGCGCGCTCACAATGCCGCTGGATGAACGTCA
GGAGCGCCAGAGGGCTCTTATGGAGCGCATACGAAAGCACGATTCCGCCCACTGGCTGAACTCCTTCATGGATGCGCTCG
AAAAGGGCGAGACAAAAGAGGACGAGGAGTAA

Upstream 100 bases:

>100_bases
CGGTTACAGGGGAGATGTGGGGCAATTACCCGCAGACCTATTCGATGGTGGGTATCATCAATGGCGCCCGCCGGCTTTCC
AAACCATGGGAGACAGTAGT

Downstream 100 bases:

>100_bases
GAGCCTCAATCCTTCTTTTTGTTCGGCAGCTTCTTCCGCTTGGTGTGAGCCAGTTCCTCAAGCTGCTTCTCGCTCATGGA
TTCTTCCATACTCTTGGAAG

Product: alpha,alpha-trehalose-phosphate synthase (UDP-forming)

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MKRLVVVSNRVADLTRTSQAGGLAVGLADSLRQRGGVWMGWDGQTLKYEEEPEPRIRKIGEVTQATIPLTEQDYAEYYLG
FANSVLWPLFHYRLDLVEYKTAFLEGYRRVNAKFANALVSILRDDDLIWIHDYHLIPLAAELRERGCKQRIGFFLHIPFP
PPEILAAVPRHRWLVECLLKHDVIGFQTHTDLNNLLRYVEDHLGCEILADGRIRTREREVIAGFYPIGIDVDAFVGMAEK
PNDEVQIETKRREILGRRQIIGVDRLDYTKGLPDRLRAFARLLELYPEMRKSVALMQIATPTREEVEAYVDIRKELEALS
GAINGRFADFNWTPVRYIHGTVPRDALAALYRASQVGFVTPLRDGMNLVAKEYVAAQSMDDPGVLVLSKFAGAAEDLHEA
LIVNPYDVDDMARNLCRALTMPLDERQERQRALMERIRKHDSAHWLNSFMDALEKGETKEDEE

Sequences:

>Translated_463_residues
MKRLVVVSNRVADLTRTSQAGGLAVGLADSLRQRGGVWMGWDGQTLKYEEEPEPRIRKIGEVTQATIPLTEQDYAEYYLG
FANSVLWPLFHYRLDLVEYKTAFLEGYRRVNAKFANALVSILRDDDLIWIHDYHLIPLAAELRERGCKQRIGFFLHIPFP
PPEILAAVPRHRWLVECLLKHDVIGFQTHTDLNNLLRYVEDHLGCEILADGRIRTREREVIAGFYPIGIDVDAFVGMAEK
PNDEVQIETKRREILGRRQIIGVDRLDYTKGLPDRLRAFARLLELYPEMRKSVALMQIATPTREEVEAYVDIRKELEALS
GAINGRFADFNWTPVRYIHGTVPRDALAALYRASQVGFVTPLRDGMNLVAKEYVAAQSMDDPGVLVLSKFAGAAEDLHEA
LIVNPYDVDDMARNLCRALTMPLDERQERQRALMERIRKHDSAHWLNSFMDALEKGETKEDEE
>Mature_463_residues
MKRLVVVSNRVADLTRTSQAGGLAVGLADSLRQRGGVWMGWDGQTLKYEEEPEPRIRKIGEVTQATIPLTEQDYAEYYLG
FANSVLWPLFHYRLDLVEYKTAFLEGYRRVNAKFANALVSILRDDDLIWIHDYHLIPLAAELRERGCKQRIGFFLHIPFP
PPEILAAVPRHRWLVECLLKHDVIGFQTHTDLNNLLRYVEDHLGCEILADGRIRTREREVIAGFYPIGIDVDAFVGMAEK
PNDEVQIETKRREILGRRQIIGVDRLDYTKGLPDRLRAFARLLELYPEMRKSVALMQIATPTREEVEAYVDIRKELEALS
GAINGRFADFNWTPVRYIHGTVPRDALAALYRASQVGFVTPLRDGMNLVAKEYVAAQSMDDPGVLVLSKFAGAAEDLHEA
LIVNPYDVDDMARNLCRALTMPLDERQERQRALMERIRKHDSAHWLNSFMDALEKGETKEDEE

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=463, Percent_Identity=47.5161987041037, Blast_Score=401, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI71987012, Length=423, Percent_Identity=32.387706855792, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI115533560, Length=362, Percent_Identity=32.5966850828729, Blast_Score=180, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI115533558, Length=362, Percent_Identity=32.5966850828729, Blast_Score=180, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6319602, Length=466, Percent_Identity=34.549356223176, Blast_Score=293, Evalue=3e-80,
Organism=Saccharomyces cerevisiae, GI6320279, Length=433, Percent_Identity=26.5588914549654, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6323537, Length=437, Percent_Identity=28.1464530892449, Blast_Score=179, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6323917, Length=445, Percent_Identity=26.0674157303371, Blast_Score=171, Evalue=3e-43,
Organism=Drosophila melanogaster, GI19920676, Length=484, Percent_Identity=37.8099173553719, Blast_Score=309, Evalue=2e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 53041; Mature: 53041

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLVVVSNRVADLTRTSQAGGLAVGLADSLRQRGGVWMGWDGQTLKYEEEPEPRIRKIG
CCCEEEECCHHHHHHHCCCCCCEEEHHHHHHHHCCCEEECCCCCEECCCCCCCHHHHHHH
EVTQATIPLTEQDYAEYYLGFANSVLWPLFHYRLDLVEYKTAFLEGYRRVNAKFANALVS
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILRDDDLIWIHDYHLIPLAAELRERGCKQRIGFFLHIPFPPPEILAAVPRHRWLVECLLK
HHCCCCEEEEECCCHHHHHHHHHHCCHHHHCCEEEECCCCCHHHHHHCCHHHHHHHHHHH
HDVIGFQTHTDLNNLLRYVEDHLGCEILADGRIRTREREVIAGFYPIGIDVDAFVGMAEK
HCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCHHHEEEEEECCCCHHHHHCCCCC
PNDEVQIETKRREILGRRQIIGVDRLDYTKGLPDRLRAFARLLELYPEMRKSVALMQIAT
CCCCEEEEHHHHHHHCHHHEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PTREEVEAYVDIRKELEALSGAINGRFADFNWTPVRYIHGTVPRDALAALYRASQVGFVT
CCHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCEEECCCCHHHHHHHHHHHCCCEEC
PLRDGMNLVAKEYVAAQSMDDPGVLVLSKFAGAAEDLHEALIVNPYDVDDMARNLCRALT
CHHHHHHHHHHHHHHHCCCCCCCEEEHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHC
MPLDERQERQRALMERIRKHDSAHWLNSFMDALEKGETKEDEE
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKRLVVVSNRVADLTRTSQAGGLAVGLADSLRQRGGVWMGWDGQTLKYEEEPEPRIRKIG
CCCEEEECCHHHHHHHCCCCCCEEEHHHHHHHHCCCEEECCCCCEECCCCCCCHHHHHHH
EVTQATIPLTEQDYAEYYLGFANSVLWPLFHYRLDLVEYKTAFLEGYRRVNAKFANALVS
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILRDDDLIWIHDYHLIPLAAELRERGCKQRIGFFLHIPFPPPEILAAVPRHRWLVECLLK
HHCCCCEEEEECCCHHHHHHHHHHCCHHHHCCEEEECCCCCHHHHHHCCHHHHHHHHHHH
HDVIGFQTHTDLNNLLRYVEDHLGCEILADGRIRTREREVIAGFYPIGIDVDAFVGMAEK
HCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCHHHEEEEEECCCCHHHHHCCCCC
PNDEVQIETKRREILGRRQIIGVDRLDYTKGLPDRLRAFARLLELYPEMRKSVALMQIAT
CCCCEEEEHHHHHHHCHHHEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PTREEVEAYVDIRKELEALSGAINGRFADFNWTPVRYIHGTVPRDALAALYRASQVGFVT
CCHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCEEECCCCHHHHHHHHHHHCCCEEC
PLRDGMNLVAKEYVAAQSMDDPGVLVLSKFAGAAEDLHEALIVNPYDVDDMARNLCRALT
CHHHHHHHHHHHHHHHCCCCCCCEEEHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHC
MPLDERQERQRALMERIRKHDSAHWLNSFMDALEKGETKEDEE
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA