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The map label for this gene is pcaF [H]

Identifier: 110633876

GI number: 110633876

Start: 1639165

End: 1640553

Strand: Direct

Name: pcaF [H]

Synonym: Meso_1523

Alternate gene names: 110633876

Gene position: 1639165-1640553 (Clockwise)

Preceding gene: 110633871

Following gene: 110633877

Centisome position: 37.15

GC content: 64.07

Gene sequence:

>1389_bases
ATGTCAGAGGCAGCCAATACAGGCACCGAGCCGGCTGTGCGGCCACGGGCCGCCCGCCGCGTTGCAGCCGCAACGACCAA
CCCCGAAGGAAAAGACGAGCGGAGGGGCAGGAAGCCGGTCTATCTGGTAGACGGCGCCCGCACCCCTTTTTTGAGGGCGA
GAGGTAAACCGGGTCCATTCACGCCGGTCGATCTTGCCGTCCAATGCGGCAGGCCTCTTCTCGACCGGCTTTCCTTCGGG
CCGGAGGAGTTCGACCTTGTCATCCTGGGATGCGTGAACGTTCTCGCCGATGAGATGAACCCCGCCCGCGTCGCCGCCCT
TCGGCTTGGCATGGGCGAGGCGATGAGAGCCTTCACGGTGCAGATCAATTGCGGCTCGGGCATGCAGTCGATCGATACGG
GTTTCCGCCTGATCGAAGGCGGCGAGGCCGACTTGATCCTGGCTGGCGGCGCTGAAGCCCTGTCCCACGCACCGCTCGTT
CTTTCCAGAAAGGCCGCCGGCTGGTTCGCACAGTTCGCCACGGCAAAGACCGCCTGGGACAGGGCCGCCGCGCTCGGCGC
GTTCCGTCCGGACATGGCGAAACCCATCATCGGGCTCGAACGCGGGCTAACCGACCCAATTACCGATTTCAGCATGGGGC
AGACCGCGGAGATCATCGGCCATGCCTTCGGCGTTACCCGGCAGGCTGCTGATGCCTATGCGCTCGAAAGCCATCGCAGG
CTCGCGCGCGCCCAGGCCGAAGGTTTTCTCAAGGGCGAGGTTATTGCCTCGGTGGCAGCCGACGGCACGCTTTACGACCA
TGACGACGGCGTAAGGCCGGATAGCTCGATTGAGAAGCTCGCAAGGTTGAAACCTGTCTTTGAACGTCCTCACGGACGCG
TGACGCCCGGCAATTCTTCCCAGATCACCGATGGGGCGAGCTGGGTCGTGATCGCATCCGAGGAGGCGATCGAAAAATAC
GGGCTCACCCCGATCGCTCGCATCGTGGACAGCGAATGGGCCGCGCTTGATCCATCCGTCATGGGCTTGGGCCCAACCAT
CTGCTCAACCGAACTCATAAAGCGGCGTGGCCTGGCGCGCGAGGACATCGACCTTTGGGAAATCAACGAGGCCTTCGCCG
CTCAGATTCTCGCCTGCCTCGCCGCATGGGAGGATGAGGAATATTGCCGCGAGGTGCTGGGGCTCAGCGTACCCTTCGGT
ACGGTTGACCGTGACCGGCTCAATGTCGACGGCGGGGCGATCAGCCTCGGCCACCCGGTGGGCTCCAGCGGCAACCGCAT
TGTGCTTCATCTTGCCAACGCCATGCGCAGGCTCGGTCTGAAGCGTGGCATCGCCACCGAGTGCATCGGGGGCGGGCAGG
GCGGCGCCATGCTTTTGGAGGTGGTCTGA

Upstream 100 bases:

>100_bases
ATGAGAGGTTTGTGAGGCTTGGCCTGACCCCGGTGGGCAAAGCCGGCAAAGGGCTCTATTGTTCCGAAGGCTACGGCCCG
GATCGAATCGAAGGGTGAAT

Downstream 100 bases:

>100_bases
TGTCTGTCTCGCGCAGGGTCTGGAAGCTCCTTCAGGATCGCAACGACTATTTCGGCCCCGAGGGAGAGCCGATGCGCCGG
CTTTCGCATTGGCGCTACAG

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MSEAANTGTEPAVRPRAARRVAAATTNPEGKDERRGRKPVYLVDGARTPFLRARGKPGPFTPVDLAVQCGRPLLDRLSFG
PEEFDLVILGCVNVLADEMNPARVAALRLGMGEAMRAFTVQINCGSGMQSIDTGFRLIEGGEADLILAGGAEALSHAPLV
LSRKAAGWFAQFATAKTAWDRAAALGAFRPDMAKPIIGLERGLTDPITDFSMGQTAEIIGHAFGVTRQAADAYALESHRR
LARAQAEGFLKGEVIASVAADGTLYDHDDGVRPDSSIEKLARLKPVFERPHGRVTPGNSSQITDGASWVVIASEEAIEKY
GLTPIARIVDSEWAALDPSVMGLGPTICSTELIKRRGLAREDIDLWEINEAFAAQILACLAAWEDEEYCREVLGLSVPFG
TVDRDRLNVDGGAISLGHPVGSSGNRIVLHLANAMRRLGLKRGIATECIGGGQGGAMLLEVV

Sequences:

>Translated_462_residues
MSEAANTGTEPAVRPRAARRVAAATTNPEGKDERRGRKPVYLVDGARTPFLRARGKPGPFTPVDLAVQCGRPLLDRLSFG
PEEFDLVILGCVNVLADEMNPARVAALRLGMGEAMRAFTVQINCGSGMQSIDTGFRLIEGGEADLILAGGAEALSHAPLV
LSRKAAGWFAQFATAKTAWDRAAALGAFRPDMAKPIIGLERGLTDPITDFSMGQTAEIIGHAFGVTRQAADAYALESHRR
LARAQAEGFLKGEVIASVAADGTLYDHDDGVRPDSSIEKLARLKPVFERPHGRVTPGNSSQITDGASWVVIASEEAIEKY
GLTPIARIVDSEWAALDPSVMGLGPTICSTELIKRRGLAREDIDLWEINEAFAAQILACLAAWEDEEYCREVLGLSVPFG
TVDRDRLNVDGGAISLGHPVGSSGNRIVLHLANAMRRLGLKRGIATECIGGGQGGAMLLEVV
>Mature_461_residues
SEAANTGTEPAVRPRAARRVAAATTNPEGKDERRGRKPVYLVDGARTPFLRARGKPGPFTPVDLAVQCGRPLLDRLSFGP
EEFDLVILGCVNVLADEMNPARVAALRLGMGEAMRAFTVQINCGSGMQSIDTGFRLIEGGEADLILAGGAEALSHAPLVL
SRKAAGWFAQFATAKTAWDRAAALGAFRPDMAKPIIGLERGLTDPITDFSMGQTAEIIGHAFGVTRQAADAYALESHRRL
ARAQAEGFLKGEVIASVAADGTLYDHDDGVRPDSSIEKLARLKPVFERPHGRVTPGNSSQITDGASWVVIASEEAIEKYG
LTPIARIVDSEWAALDPSVMGLGPTICSTELIKRRGLAREDIDLWEINEAFAAQILACLAAWEDEEYCREVLGLSVPFGT
VDRDRLNVDGGAISLGHPVGSSGNRIVLHLANAMRRLGLKRGIATECIGGGQGGAMLLEVV

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4504327, Length=454, Percent_Identity=35.9030837004405, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI167614485, Length=428, Percent_Identity=35.5140186915888, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI148539872, Length=429, Percent_Identity=33.3333333333333, Blast_Score=205, Evalue=7e-53,
Organism=Homo sapiens, GI4557237, Length=428, Percent_Identity=29.9065420560748, Blast_Score=179, Evalue=4e-45,
Organism=Homo sapiens, GI4501853, Length=436, Percent_Identity=30.2752293577982, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI194353979, Length=252, Percent_Identity=27.7777777777778, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1787663, Length=433, Percent_Identity=36.7205542725173, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI87082165, Length=428, Percent_Identity=34.3457943925234, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI48994986, Length=432, Percent_Identity=36.1111111111111, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1788554, Length=426, Percent_Identity=34.5070422535211, Blast_Score=208, Evalue=6e-55,
Organism=Escherichia coli, GI1788683, Length=429, Percent_Identity=32.4009324009324, Blast_Score=190, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI133906874, Length=434, Percent_Identity=35.4838709677419, Blast_Score=231, Evalue=5e-61,
Organism=Caenorhabditis elegans, GI17551802, Length=431, Percent_Identity=32.7146171693735, Blast_Score=218, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI25147385, Length=428, Percent_Identity=29.9065420560748, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17535921, Length=426, Percent_Identity=31.4553990610329, Blast_Score=165, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17535917, Length=466, Percent_Identity=26.1802575107296, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6325229, Length=440, Percent_Identity=31.3636363636364, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6322031, Length=358, Percent_Identity=28.4916201117318, Blast_Score=156, Evalue=8e-39,
Organism=Drosophila melanogaster, GI17137578, Length=452, Percent_Identity=37.6106194690265, Blast_Score=272, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24655093, Length=424, Percent_Identity=33.2547169811321, Blast_Score=233, Evalue=1e-61,
Organism=Drosophila melanogaster, GI17648125, Length=432, Percent_Identity=35.6481481481481, Blast_Score=217, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24640423, Length=425, Percent_Identity=31.5294117647059, Blast_Score=196, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 49212; Mature: 49081

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00737 THIOLASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAANTGTEPAVRPRAARRVAAATTNPEGKDERRGRKPVYLVDGARTPFLRARGKPGPF
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHCCCCCEEEECCCCCCHHCCCCCCCCC
TPVDLAVQCGRPLLDRLSFGPEEFDLVILGCVNVLADEMNPARVAALRLGMGEAMRAFTV
CHHHHHHHCCHHHHHHHCCCCCHHCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEE
QINCGSGMQSIDTGFRLIEGGEADLILAGGAEALSHAPLVLSRKAAGWFAQFATAKTAWD
EEECCCCCHHHHCCCEEEECCCCCEEEECCHHHHHCCCEEEECCCHHHHHHHHHHHHHHH
RAAALGAFRPDMAKPIIGLERGLTDPITDFSMGQTAEIIGHAFGVTRQAADAYALESHRR
HHHHHCCCCCHHHCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LARAQAEGFLKGEVIASVAADGTLYDHDDGVRPDSSIEKLARLKPVFERPHGRVTPGNSS
HHHHHHCCCHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
QITDGASWVVIASEEAIEKYGLTPIARIVDSEWAALDPSVMGLGPTICSTELIKRRGLAR
CCCCCCCEEEEECHHHHHHHCCCHHHHHHCCCHHHCCCCHHCCCCHHHHHHHHHHCCCCC
EDIDLWEINEAFAAQILACLAAWEDEEYCREVLGLSVPFGTVDRDRLNVDGGAISLGHPV
CCCCCEECCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEECCCCEEEECCCC
GSSGNRIVLHLANAMRRLGLKRGIATECIGGGQGGAMLLEVV
CCCCCEEEEEHHHHHHHHHHHCCCHHHHCCCCCCCEEEEEEC
>Mature Secondary Structure 
SEAANTGTEPAVRPRAARRVAAATTNPEGKDERRGRKPVYLVDGARTPFLRARGKPGPF
CCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHCCCCCEEEECCCCCCHHCCCCCCCCC
TPVDLAVQCGRPLLDRLSFGPEEFDLVILGCVNVLADEMNPARVAALRLGMGEAMRAFTV
CHHHHHHHCCHHHHHHHCCCCCHHCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEE
QINCGSGMQSIDTGFRLIEGGEADLILAGGAEALSHAPLVLSRKAAGWFAQFATAKTAWD
EEECCCCCHHHHCCCEEEECCCCCEEEECCHHHHHCCCEEEECCCHHHHHHHHHHHHHHH
RAAALGAFRPDMAKPIIGLERGLTDPITDFSMGQTAEIIGHAFGVTRQAADAYALESHRR
HHHHHCCCCCHHHCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LARAQAEGFLKGEVIASVAADGTLYDHDDGVRPDSSIEKLARLKPVFERPHGRVTPGNSS
HHHHHHCCCHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
QITDGASWVVIASEEAIEKYGLTPIARIVDSEWAALDPSVMGLGPTICSTELIKRRGLAR
CCCCCCCEEEEECHHHHHHHCCCHHHHHHCCCHHHCCCCHHCCCCHHHHHHHHHHCCCCC
EDIDLWEINEAFAAQILACLAAWEDEEYCREVLGLSVPFGTVDRDRLNVDGGAISLGHPV
CCCCCEECCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEECCCCEEEECCCC
GSSGNRIVLHLANAMRRLGLKRGIATECIGGGQGGAMLLEVV
CCCCCEEEEEHHHHHHHHHHHCCCHHHHCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]