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The map label for this gene is gatA

Identifier: 110633718

GI number: 110633718

Start: 1457103

End: 1458584

Strand: Direct

Name: gatA

Synonym: Meso_1365

Alternate gene names: 110633718

Gene position: 1457103-1458584 (Clockwise)

Preceding gene: 110633717

Following gene: 110633719

Centisome position: 33.02

GC content: 63.09

Gene sequence:

>1482_bases
ATGACCGAGCTTACGAAACTCACCATTTCGGAAATCCGAGCCAAACTGGCCGACAAGGAGTTCACGGCGCTGGAATTGAC
GGAGGCGTATCTTTCGGCAATCCAAACGGCGAATGAGAAGCTGAACGCCTATATCACGGTGACGGCGGACAAGGCGCGGG
AGATGGCCAAGGCTTCGGACATGCGCCTCATGCAGGGCGGCGCGCGGCCGCTTGAAGGCGTGCCTCTCGGAATCAAGGAC
CTTTTCGCCACGGAAGGCGTGCACACCCAGGCCTGCAGCCACATTCTCGATGCGTTCAAGCCCCGTTACGAATCGACTGT
CACGACCAATCTCTGGGCCGATGGCGCAGTGATGCTCGGCAAGCTCAACATGGACGAGTTCGCCATGGGCTCCTCCAACG
AGACTTCTTACTACGGCCCGGTGGTAAACCCTTGGCGCGAGGAAGGCGGCAACAAGGATCTCGTGCCCGGCGGCTCTTCG
GGCGGCTCGGCCGCGGCGGTTGCGGCCTGGCTTTGCGCGGGAGCCACCGCGACCGATACGGGCGGGTCGATCCGGCAACC
GGCGGCCTTCACCGCGACGGTGGGCATAAAGCCCACTTATGGGCGCTGCTCGCGGTGGGGGATCGTCGCCTTCGCATCCT
CGCTGGATCAGGCGGGGCCGATCGCGCGCGATGTGCGGGACGCGGCAATCCTTCTGAAGTCCATGGCCTCGGTCGATCCC
AAGGATACGACGTCGATCGACATCCCCGTGCCGGATTACGAGGCCGCGATCGGCCAGTCGGTGAAAGGGCTGAAGATCGG
CATTCCGAAGGAGTACCGGGTCGAAGGCATGCCGGCCGAGATCGAGGCGCTTTGGCATAAAGGAATCGAGTGGATGCGCG
ATGCCGGCGCCGAGATCGTCGATATCTCTCTGCCGCACACGAAATACGCGCTGCCGGCCTATTATATCGTCGCGCCGGCG
GAGGCTTCGTCAAACCTGGCGCGCTATGACGGCGTGCGTTACGGGCTGCGGGTGCCTGGCCGCGACATCACCGAGATGTA
CGAGAATTCGCGGGCGGCGGGCTTCGGTCGCGAGGTCAAGCGGCGCATCATGATCGGCACCTATGTGCTGTCTGCCGGTT
ATTACGATGCCTATTACCTGCGCGCGCAGAGGGTGCGCACGCTGATCAAGCGCGATTTTGAGCAGGCCTTCGAGGCGGGC
GTTGATGCGATCCTCACACCGGCGACCCCTTCGGCGGCGTTCGGCATTGCCGACCAGGAAATGAACGCGGATCCTGTGAA
GATGTACCTGAACGACATTTTCACCGTGACGGTGAACATGGCGGGCCTGCCAGGCATCGCCGTACCGGCGGGGCTCGATT
CCAGGGGCCTGCCGCTCGGACTTCAGCTCATTGGCCGCGCCTTCGACGAGGAGACGCTGTTCCGCACGGCTCACATAATC
GAGCAGGCCGCGGGTAAATTCGAGCCGAAGCGCTGGTGGTAA

Upstream 100 bases:

>100_bases
ACCCGCTGCTGCCCCGTGCTCGATTATCCTGACTCTCAATGGTCCGTCTTTTACGAAAAAAACCTCTCCGCACAATCCGC
GGCTGCCTGAACGGAAGAAT

Downstream 100 bases:

>100_bases
TTGCGCCCGAGGTGACGCTGGGGCGTACTCTCGAGCACGATCTTCGTGATTCGGCTTGAGGGCGAAATATGTTCCACCTG
GCCTCCGCCATTTTTTGGGC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MTELTKLTISEIRAKLADKEFTALELTEAYLSAIQTANEKLNAYITVTADKAREMAKASDMRLMQGGARPLEGVPLGIKD
LFATEGVHTQACSHILDAFKPRYESTVTTNLWADGAVMLGKLNMDEFAMGSSNETSYYGPVVNPWREEGGNKDLVPGGSS
GGSAAAVAAWLCAGATATDTGGSIRQPAAFTATVGIKPTYGRCSRWGIVAFASSLDQAGPIARDVRDAAILLKSMASVDP
KDTTSIDIPVPDYEAAIGQSVKGLKIGIPKEYRVEGMPAEIEALWHKGIEWMRDAGAEIVDISLPHTKYALPAYYIVAPA
EASSNLARYDGVRYGLRVPGRDITEMYENSRAAGFGREVKRRIMIGTYVLSAGYYDAYYLRAQRVRTLIKRDFEQAFEAG
VDAILTPATPSAAFGIADQEMNADPVKMYLNDIFTVTVNMAGLPGIAVPAGLDSRGLPLGLQLIGRAFDEETLFRTAHII
EQAAGKFEPKRWW

Sequences:

>Translated_493_residues
MTELTKLTISEIRAKLADKEFTALELTEAYLSAIQTANEKLNAYITVTADKAREMAKASDMRLMQGGARPLEGVPLGIKD
LFATEGVHTQACSHILDAFKPRYESTVTTNLWADGAVMLGKLNMDEFAMGSSNETSYYGPVVNPWREEGGNKDLVPGGSS
GGSAAAVAAWLCAGATATDTGGSIRQPAAFTATVGIKPTYGRCSRWGIVAFASSLDQAGPIARDVRDAAILLKSMASVDP
KDTTSIDIPVPDYEAAIGQSVKGLKIGIPKEYRVEGMPAEIEALWHKGIEWMRDAGAEIVDISLPHTKYALPAYYIVAPA
EASSNLARYDGVRYGLRVPGRDITEMYENSRAAGFGREVKRRIMIGTYVLSAGYYDAYYLRAQRVRTLIKRDFEQAFEAG
VDAILTPATPSAAFGIADQEMNADPVKMYLNDIFTVTVNMAGLPGIAVPAGLDSRGLPLGLQLIGRAFDEETLFRTAHII
EQAAGKFEPKRWW
>Mature_492_residues
TELTKLTISEIRAKLADKEFTALELTEAYLSAIQTANEKLNAYITVTADKAREMAKASDMRLMQGGARPLEGVPLGIKDL
FATEGVHTQACSHILDAFKPRYESTVTTNLWADGAVMLGKLNMDEFAMGSSNETSYYGPVVNPWREEGGNKDLVPGGSSG
GSAAAVAAWLCAGATATDTGGSIRQPAAFTATVGIKPTYGRCSRWGIVAFASSLDQAGPIARDVRDAAILLKSMASVDPK
DTTSIDIPVPDYEAAIGQSVKGLKIGIPKEYRVEGMPAEIEALWHKGIEWMRDAGAEIVDISLPHTKYALPAYYIVAPAE
ASSNLARYDGVRYGLRVPGRDITEMYENSRAAGFGREVKRRIMIGTYVLSAGYYDAYYLRAQRVRTLIKRDFEQAFEAGV
DAILTPATPSAAFGIADQEMNADPVKMYLNDIFTVTVNMAGLPGIAVPAGLDSRGLPLGLQLIGRAFDEETLFRTAHIIE
QAAGKFEPKRWW

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=494, Percent_Identity=40.8906882591093, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI195972892, Length=518, Percent_Identity=22.7799227799228, Blast_Score=103, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17543272, Length=441, Percent_Identity=37.6417233560091, Blast_Score=279, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17537465, Length=502, Percent_Identity=24.7011952191235, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17538252, Length=268, Percent_Identity=26.4925373134328, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=360, Percent_Identity=26.1111111111111, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17556264, Length=191, Percent_Identity=28.2722513089005, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=426, Percent_Identity=35.6807511737089, Blast_Score=254, Evalue=3e-68,
Organism=Saccharomyces cerevisiae, GI6319685, Length=422, Percent_Identity=27.0142180094787, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320448, Length=247, Percent_Identity=27.1255060728745, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24648113, Length=487, Percent_Identity=39.2197125256673, Blast_Score=301, Evalue=5e-82,
Organism=Drosophila melanogaster, GI24644968, Length=520, Percent_Identity=23.4615384615385, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24652985, Length=492, Percent_Identity=22.7642276422764, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19922090, Length=492, Percent_Identity=22.7642276422764, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24652981, Length=492, Percent_Identity=22.7642276422764, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24652983, Length=492, Percent_Identity=22.7642276422764, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI161078093, Length=266, Percent_Identity=28.1954887218045, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI45550774, Length=252, Percent_Identity=26.1904761904762, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24648435, Length=252, Percent_Identity=26.1904761904762, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24648437, Length=252, Percent_Identity=26.1904761904762, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21356731, Length=273, Percent_Identity=26.7399267399267, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_MESSB (Q11IL4)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_673926.1
- ProteinModelPortal:   Q11IL4
- SMR:   Q11IL4
- STRING:   Q11IL4
- GeneID:   4181560
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_1365
- NMPDR:   fig|266779.1.peg.2227
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- PhylomeDB:   Q11IL4
- ProtClustDB:   PRK00012
- BioCyc:   MSP266779:MESO_1365-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 53373; Mature: 53242

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 79-79 ACT_SITE 159-159 ACT_SITE 183-183

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELTKLTISEIRAKLADKEFTALELTEAYLSAIQTANEKLNAYITVTADKAREMAKASD
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHH
MRLMQGGARPLEGVPLGIKDLFATEGVHTQACSHILDAFKPRYESTVTTNLWADGAVMLG
HHHHHCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCHHCCCEEEEECCCCEEEEE
KLNMDEFAMGSSNETSYYGPVVNPWREEGGNKDLVPGGSSGGSAAAVAAWLCAGATATDT
ECCHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCC
GGSIRQPAAFTATVGIKPTYGRCSRWGIVAFASSLDQAGPIARDVRDAAILLKSMASVDP
CCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
KDTTSIDIPVPDYEAAIGQSVKGLKIGIPKEYRVEGMPAEIEALWHKGIEWMRDAGAEIV
CCCCEEECCCCCHHHHHCCCCCCEEECCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCEEE
DISLPHTKYALPAYYIVAPAEASSNLARYDGVRYGLRVPGRDITEMYENSRAAGFGREVK
EEECCCCCCCCCEEEEEECCCCCCCHHHHCCCEEEEECCCCHHHHHHHCCCCCCCCHHHH
RRIMIGTYVLSAGYYDAYYLRAQRVRTLIKRDFEQAFEAGVDAILTPATPSAAFGIADQE
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHCCCCCCC
MNADPVKMYLNDIFTVTVNMAGLPGIAVPAGLDSRGLPLGLQLIGRAFDEETLFRTAHII
CCCCHHHHHHHHEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHH
EQAAGKFEPKRWW
HHHHCCCCCCCCC
>Mature Secondary Structure 
TELTKLTISEIRAKLADKEFTALELTEAYLSAIQTANEKLNAYITVTADKAREMAKASD
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHH
MRLMQGGARPLEGVPLGIKDLFATEGVHTQACSHILDAFKPRYESTVTTNLWADGAVMLG
HHHHHCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCHHCCCEEEEECCCCEEEEE
KLNMDEFAMGSSNETSYYGPVVNPWREEGGNKDLVPGGSSGGSAAAVAAWLCAGATATDT
ECCHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCC
GGSIRQPAAFTATVGIKPTYGRCSRWGIVAFASSLDQAGPIARDVRDAAILLKSMASVDP
CCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
KDTTSIDIPVPDYEAAIGQSVKGLKIGIPKEYRVEGMPAEIEALWHKGIEWMRDAGAEIV
CCCCEEECCCCCHHHHHCCCCCCEEECCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCEEE
DISLPHTKYALPAYYIVAPAEASSNLARYDGVRYGLRVPGRDITEMYENSRAAGFGREVK
EEECCCCCCCCCEEEEEECCCCCCCHHHHCCCEEEEECCCCHHHHHHHCCCCCCCCHHHH
RRIMIGTYVLSAGYYDAYYLRAQRVRTLIKRDFEQAFEAGVDAILTPATPSAAFGIADQE
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHCCCCCCC
MNADPVKMYLNDIFTVTVNMAGLPGIAVPAGLDSRGLPLGLQLIGRAFDEETLFRTAHII
CCCCHHHHHHHHEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHH
EQAAGKFEPKRWW
HHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA