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The map label for this gene is pleD [H]

Identifier: 110633701

GI number: 110633701

Start: 1440155

End: 1441528

Strand: Direct

Name: pleD [H]

Synonym: Meso_1348

Alternate gene names: 110633701

Gene position: 1440155-1441528 (Clockwise)

Preceding gene: 110633700

Following gene: 110633702

Centisome position: 32.64

GC content: 60.92

Gene sequence:

>1374_bases
ATGACTGCCCGTATCCTTGTGGTGGATGATGTTCCTGCCAATCTGCGCCTGCTGGAGGCACGGCTCCTCGCCGAATATTA
CGACGTGGTGACCGCCCGTTCGGGACCGGACGCCATCGATATCTGCGAATCAAGCAAGATCGACGTTGTGCTCCTGGACG
TGATGATGCCGGGAATGGACGGCTTCGAGGTCTGCCGGCGCTTGAAAGCCGATCCGCTCACGACGCATATCCCCGTGATC
ATGATCACCGCGCTCGACCAGCCGTCGGATCGCGTCCGCGGCTTGGAGGCCGGAGCCGATGATTTTCTGACGAAGCCAGT
CAACGACCTGCAGCTTCTGACGCGTGTAAAGAGCCTAACCAGGCTCAAGGCATTGACGGACGAGCTCAGGCTGCGCGCGG
CTACGACGCGCAACATCGGCATTGAGCAGCTGCTGCAGAAGAAGAGTGTCGATGACGAGGAAAAGCCTTCGGTGCTCCTC
ATCGATGAGGATAAAGCTGCCGGCAGGCAGCTTGCCAGAATGCTGCAGGATCATTTTGCGCTGGATGCCGTCGACGACCC
GCAGGAAGGCTTGGTTCGCGCCGCCGAAAGGTCTTACGAGTGCATCATCGTATCGAGCAGCTTTGTTGCCTATGACCCGC
TGCGGCTGTGCTCGCAGCTAAGGGCGATGGACCGCACGCGGCTCGTTCCCATCATCCTCTTTGCGGGCGAGGGCGATGAG
ACAGTGATCATTCGCGGGCTCGAACTGGCGGTGAATGATTATGTGCTGCGCCCGGTGGAACGGCAGGAACTGATCGCGCG
GCTGCGCACGCAGATCCGCCGCAAGCGATACAATGACAGCCTGCGCTCCAGTGTCGCCCAGACTGTGGAAATGGCGATCA
CCGATCCGCTGACCGGCCTTCATAACCGGCGGTATCTTGATAGTCACCTTCAGAGCCTTTTCGACCGCGCGGAGGCCAGG
CGCCGTCCGCTTTCGGTCATGATCGTCGATATCGACCGCTTCAAGTCAATCAACGACACCTACGGACATGATGGCGGTGA
CGCGGTATTGCGCGCGGTGGCCGCGCGGCTGCGCCGGAGCCTGCGTGGGATCGATCTCGTGTGCCGTTACGGCGGTGAAG
AATTCGTGGTTGTGATGCCGGAAACGGAACTTTCGATCGCAGAGCGCGTCGGCGAGCGCATCAGGCAGCAGATCGCCGAA
GGCGCTTTTTTGCTGGGCGATTCAGCCGGTGCCATTTCTGTCACGGTCAGCATAGGGGTGGCGGCGCTTCTCCAGCCGCA
GGATACCGTGGAGGCGCTGATGAAGCGCGCTGATATGGCGCTTTATGAGGCGAAGTCGAGCGGTCGCAATCGGGTGGTCG
CACCGGCTGCCTGA

Upstream 100 bases:

>100_bases
AAGCCGATCTCGGTGCAGAGCTTTATCGAGACAATCAAATCCTATCTCGGGGACGCCTGATGCGCGCCCAGACTTATTCC
AGCGAGAGCGACCGAGCGCT

Downstream 100 bases:

>100_bases
TCCATAACCGCGCCGGTTCTTAACGGCATTGCGGGCTGGCAAATCAAGCCGAAATGCGTCGGCGTCAGCGGCCAAGTTTC
CGAGATGAAGCCCCTGAAGC

Product: response regulator PleD

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MTARILVVDDVPANLRLLEARLLAEYYDVVTARSGPDAIDICESSKIDVVLLDVMMPGMDGFEVCRRLKADPLTTHIPVI
MITALDQPSDRVRGLEAGADDFLTKPVNDLQLLTRVKSLTRLKALTDELRLRAATTRNIGIEQLLQKKSVDDEEKPSVLL
IDEDKAAGRQLARMLQDHFALDAVDDPQEGLVRAAERSYECIIVSSSFVAYDPLRLCSQLRAMDRTRLVPIILFAGEGDE
TVIIRGLELAVNDYVLRPVERQELIARLRTQIRRKRYNDSLRSSVAQTVEMAITDPLTGLHNRRYLDSHLQSLFDRAEAR
RRPLSVMIVDIDRFKSINDTYGHDGGDAVLRAVAARLRRSLRGIDLVCRYGGEEFVVVMPETELSIAERVGERIRQQIAE
GAFLLGDSAGAISVTVSIGVAALLQPQDTVEALMKRADMALYEAKSSGRNRVVAPAA

Sequences:

>Translated_457_residues
MTARILVVDDVPANLRLLEARLLAEYYDVVTARSGPDAIDICESSKIDVVLLDVMMPGMDGFEVCRRLKADPLTTHIPVI
MITALDQPSDRVRGLEAGADDFLTKPVNDLQLLTRVKSLTRLKALTDELRLRAATTRNIGIEQLLQKKSVDDEEKPSVLL
IDEDKAAGRQLARMLQDHFALDAVDDPQEGLVRAAERSYECIIVSSSFVAYDPLRLCSQLRAMDRTRLVPIILFAGEGDE
TVIIRGLELAVNDYVLRPVERQELIARLRTQIRRKRYNDSLRSSVAQTVEMAITDPLTGLHNRRYLDSHLQSLFDRAEAR
RRPLSVMIVDIDRFKSINDTYGHDGGDAVLRAVAARLRRSLRGIDLVCRYGGEEFVVVMPETELSIAERVGERIRQQIAE
GAFLLGDSAGAISVTVSIGVAALLQPQDTVEALMKRADMALYEAKSSGRNRVVAPAA
>Mature_456_residues
TARILVVDDVPANLRLLEARLLAEYYDVVTARSGPDAIDICESSKIDVVLLDVMMPGMDGFEVCRRLKADPLTTHIPVIM
ITALDQPSDRVRGLEAGADDFLTKPVNDLQLLTRVKSLTRLKALTDELRLRAATTRNIGIEQLLQKKSVDDEEKPSVLLI
DEDKAAGRQLARMLQDHFALDAVDDPQEGLVRAAERSYECIIVSSSFVAYDPLRLCSQLRAMDRTRLVPIILFAGEGDET
VIIRGLELAVNDYVLRPVERQELIARLRTQIRRKRYNDSLRSSVAQTVEMAITDPLTGLHNRRYLDSHLQSLFDRAEARR
RPLSVMIVDIDRFKSINDTYGHDGGDAVLRAVAARLRRSLRGIDLVCRYGGEEFVVVMPETELSIAERVGERIRQQIAEG
AFLLGDSAGAISVTVSIGVAALLQPQDTVEALMKRADMALYEAKSSGRNRVVAPAA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=42.4050632911392, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI1786584, Length=161, Percent_Identity=37.888198757764, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787262, Length=175, Percent_Identity=38.8571428571429, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI87082007, Length=164, Percent_Identity=41.4634146341463, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI87081881, Length=168, Percent_Identity=42.2619047619048, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1787816, Length=159, Percent_Identity=42.1383647798742, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI87081977, Length=198, Percent_Identity=33.3333333333333, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787802, Length=161, Percent_Identity=37.888198757764, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1788085, Length=168, Percent_Identity=33.9285714285714, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1788381, Length=172, Percent_Identity=30.8139534883721, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1786599, Length=122, Percent_Identity=39.344262295082, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1789809, Length=122, Percent_Identity=38.5245901639344, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1787541, Length=165, Percent_Identity=34.5454545454545, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI145693157, Length=103, Percent_Identity=44.6601941747573, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788394, Length=120, Percent_Identity=36.6666666666667, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788956, Length=175, Percent_Identity=33.1428571428571, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081974, Length=159, Percent_Identity=29.559748427673, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1790863, Length=118, Percent_Identity=33.0508474576271, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87082012, Length=118, Percent_Identity=33.8983050847458, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1786784, Length=118, Percent_Identity=32.2033898305085, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1787229, Length=119, Percent_Identity=32.7731092436975, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789149, Length=108, Percent_Identity=34.2592592592593, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI2367329, Length=125, Percent_Identity=37.6, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790860, Length=100, Percent_Identity=38, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1787056, Length=168, Percent_Identity=32.7380952380952, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788905, Length=170, Percent_Identity=32.9411764705882, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1790437, Length=104, Percent_Identity=38.4615384615385, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788550, Length=132, Percent_Identity=31.8181818181818, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788713, Length=126, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 50812; Mature: 50681

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARILVVDDVPANLRLLEARLLAEYYDVVTARSGPDAIDICESSKIDVVLLDVMMPGMD
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEEEHHCCCCC
GFEVCRRLKADPLTTHIPVIMITALDQPSDRVRGLEAGADDFLTKPVNDLQLLTRVKSLT
HHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHH
RLKALTDELRLRAATTRNIGIEQLLQKKSVDDEEKPSVLLIDEDKAAGRQLARMLQDHFA
HHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHC
LDAVDDPQEGLVRAAERSYECIIVSSSFVAYDPLRLCSQLRAMDRTRLVPIILFAGEGDE
CCCCCCCHHHHHHHHCCCCEEEEEECCCHHCCHHHHHHHHHHHCCCCEEEEEEEECCCCC
TVIIRGLELAVNDYVLRPVERQELIARLRTQIRRKRYNDSLRSSVAQTVEMAITDPLTGL
EEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHC
HNRRYLDSHLQSLFDRAEARRRPLSVMIVDIDRFKSINDTYGHDGGDAVLRAVAARLRRS
CCHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
LRGIDLVCRYGGEEFVVVMPETELSIAERVGERIRQQIAEGAFLLGDSAGAISVTVSIGV
HCCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEEEEHHH
AALLQPQDTVEALMKRADMALYEAKSSGRNRVVAPAA
HHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCCC
>Mature Secondary Structure 
TARILVVDDVPANLRLLEARLLAEYYDVVTARSGPDAIDICESSKIDVVLLDVMMPGMD
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEEEHHCCCCC
GFEVCRRLKADPLTTHIPVIMITALDQPSDRVRGLEAGADDFLTKPVNDLQLLTRVKSLT
HHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHH
RLKALTDELRLRAATTRNIGIEQLLQKKSVDDEEKPSVLLIDEDKAAGRQLARMLQDHFA
HHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHC
LDAVDDPQEGLVRAAERSYECIIVSSSFVAYDPLRLCSQLRAMDRTRLVPIILFAGEGDE
CCCCCCCHHHHHHHHCCCCEEEEEECCCHHCCHHHHHHHHHHHCCCCEEEEEEEECCCCC
TVIIRGLELAVNDYVLRPVERQELIARLRTQIRRKRYNDSLRSSVAQTVEMAITDPLTGL
EEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHC
HNRRYLDSHLQSLFDRAEARRRPLSVMIVDIDRFKSINDTYGHDGGDAVLRAVAARLRRS
CCHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
LRGIDLVCRYGGEEFVVVMPETELSIAERVGERIRQQIAEGAFLLGDSAGAISVTVSIGV
HCCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEEEEHHH
AALLQPQDTVEALMKRADMALYEAKSSGRNRVVAPAA
HHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA