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The map label for this gene is purB [H]

Identifier: 110633638

GI number: 110633638

Start: 1377327

End: 1378634

Strand: Reverse

Name: purB [H]

Synonym: Meso_1285

Alternate gene names: 110633638

Gene position: 1378634-1377327 (Counterclockwise)

Preceding gene: 110633639

Following gene: 110633636

Centisome position: 31.24

GC content: 61.16

Gene sequence:

>1308_bases
ATGATCCCCCGCTATTCCCGGCCCGAAATGGTATCCATCTGGTCTCCCGAGACGCGATTCCGCATCTGGTTCGAGATCGA
GGCCCATGCGGCGGATGCGATGGCGAAACTCGGCATCGTGCCGCAGGAAGCGGCCAGGACGATTTGGGAAAAAGGCGGCA
ATGCCACTTTTGATATCGACCGGATCGACGAGATCGAGCGCGAGACGAAGCACGATGTCATCGCGTTTCTGACGCATCTG
TCGGAGATCGTCGGCCCCGAGGCGCGCTTCGTGCATCAAGGCATGACCTCCTCGGACGTGCTCGACACCTGCCTTAACGT
ACAACTGGTCCGGGCAAGCGATATCCTGCTCGACGATCTCGACAGGCTGCTGGAAGTCCTCAAGAAGCGCGCATTCGAAC
ACAAGGACACCGTCACCATCGGCCGGAGCCATGGCATACATGCCGAGCCAACCACCTTCGGAATCAAGCTCGCGCAGGCC
TATGCGGAGTTCTCCCGCTGCCGCGAAAGGCTGGAGCATGCGCGCGAGGAAGTCGCCACCTGCGCCATCTCCGGCGCAGT
CGGCACGTTTGCCAATATCGATCCACGTGTGGAAGAGCATGTGGCCGAGAAAATGGGGCTGAAGCCGGAGCCGGTCTCGA
CCCAGGTCATCCCGCGCGACCGGCACGCGATGTTCTTCGCCACACTGGCGGTGATTGCCTCCTCAATCGAGCGGCTGGCC
ACGGAAATACGCCATCTTCAGCGCACGGAAGTTCTGGAAGCGGAGGAATATTTCTCGCCGGGGCAGAAGGGTTCGTCCGC
CATGCCGCACAAGCGCAACCCGGTGCTTTCGGAAAACCTCACGGGTCTTGCCCGGATGGTGCGTTCTTATGCAATGCCGG
CCATGGAAAATGTCGCGCTCTGGCATGAGCGGGACATCTCCCACTCCTCCGTCGAACGCATGATCGGCCCCGATGCGACG
GTGACGCTCGATTTCGCGCTGGTACGGCTCACCGGGCTCATGGAAAAGCTGGTGGTCTATCCGGAGAACATGCTCAGGAA
CCTAGACCGCTTCCGCGGGCTCGTCCACTCCCAGCGTGTGCTTCTGGCACTCACCCAAGCGGGCGTGAGCCGCGAGGACG
CCTACCGGCTGGTCCAACGCAACGCCATGAAGGTATGGGAGCATGGGGCGGATTTCCAGCAGGAACTGCTCGCCGATAAG
GATGTGCGCGCCGCGCTCTCGGAAGCCGAGATCCGCGAGAAATTCGACCTCGGCTACCACACCAAGCACGTGGACACGAT
TTTCAAGCGGGTTTTCGGAAAGGCCTAA

Upstream 100 bases:

>100_bases
ACCGTCCGTCTTATCGGCACGATAGCCATGTGCGTTGAGCTTCGCGCGGCTGTCTGATAGGACGCGACGCAACTCCTTTC
CAATCTATGCAGGCAGTTCC

Downstream 100 bases:

>100_bases
GCCTTCCCGCTTACCCGGATGACGGTCCCCCACGGGTCTTCCAGCGCGCGTTCGCCCTCGGCGCCAGCCGCGCTGAGCTG
AACCCAGGACAAGCCCGCGC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MIPRYSRPEMVSIWSPETRFRIWFEIEAHAADAMAKLGIVPQEAARTIWEKGGNATFDIDRIDEIERETKHDVIAFLTHL
SEIVGPEARFVHQGMTSSDVLDTCLNVQLVRASDILLDDLDRLLEVLKKRAFEHKDTVTIGRSHGIHAEPTTFGIKLAQA
YAEFSRCRERLEHAREEVATCAISGAVGTFANIDPRVEEHVAEKMGLKPEPVSTQVIPRDRHAMFFATLAVIASSIERLA
TEIRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLSENLTGLARMVRSYAMPAMENVALWHERDISHSSVERMIGPDAT
VTLDFALVRLTGLMEKLVVYPENMLRNLDRFRGLVHSQRVLLALTQAGVSREDAYRLVQRNAMKVWEHGADFQQELLADK
DVRAALSEAEIREKFDLGYHTKHVDTIFKRVFGKA

Sequences:

>Translated_435_residues
MIPRYSRPEMVSIWSPETRFRIWFEIEAHAADAMAKLGIVPQEAARTIWEKGGNATFDIDRIDEIERETKHDVIAFLTHL
SEIVGPEARFVHQGMTSSDVLDTCLNVQLVRASDILLDDLDRLLEVLKKRAFEHKDTVTIGRSHGIHAEPTTFGIKLAQA
YAEFSRCRERLEHAREEVATCAISGAVGTFANIDPRVEEHVAEKMGLKPEPVSTQVIPRDRHAMFFATLAVIASSIERLA
TEIRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLSENLTGLARMVRSYAMPAMENVALWHERDISHSSVERMIGPDAT
VTLDFALVRLTGLMEKLVVYPENMLRNLDRFRGLVHSQRVLLALTQAGVSREDAYRLVQRNAMKVWEHGADFQQELLADK
DVRAALSEAEIREKFDLGYHTKHVDTIFKRVFGKA
>Mature_435_residues
MIPRYSRPEMVSIWSPETRFRIWFEIEAHAADAMAKLGIVPQEAARTIWEKGGNATFDIDRIDEIERETKHDVIAFLTHL
SEIVGPEARFVHQGMTSSDVLDTCLNVQLVRASDILLDDLDRLLEVLKKRAFEHKDTVTIGRSHGIHAEPTTFGIKLAQA
YAEFSRCRERLEHAREEVATCAISGAVGTFANIDPRVEEHVAEKMGLKPEPVSTQVIPRDRHAMFFATLAVIASSIERLA
TEIRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLSENLTGLARMVRSYAMPAMENVALWHERDISHSSVERMIGPDAT
VTLDFALVRLTGLMEKLVVYPENMLRNLDRFRGLVHSQRVLLALTQAGVSREDAYRLVQRNAMKVWEHGADFQQELLADK
DVRAALSEAEIREKFDLGYHTKHVDTIFKRVFGKA

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=404, Percent_Identity=29.7029702970297, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI183227688, Length=381, Percent_Identity=29.6587926509186, Blast_Score=143, Evalue=3e-34,
Organism=Escherichia coli, GI1787376, Length=352, Percent_Identity=27.8409090909091, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1787896, Length=250, Percent_Identity=26, Blast_Score=65, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17508577, Length=400, Percent_Identity=28, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI32564234, Length=367, Percent_Identity=28.6103542234332, Blast_Score=102, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6323391, Length=386, Percent_Identity=29.0155440414508, Blast_Score=136, Evalue=6e-33,
Organism=Drosophila melanogaster, GI24647570, Length=405, Percent_Identity=27.6543209876543, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24640177, Length=207, Percent_Identity=28.5024154589372, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24640179, Length=207, Percent_Identity=28.5024154589372, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI78710009, Length=207, Percent_Identity=28.0193236714976, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49298; Mature: 49298

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPRYSRPEMVSIWSPETRFRIWFEIEAHAADAMAKLGIVPQEAARTIWEKGGNATFDID
CCCCCCCCCEEEEECCCCEEEEEEEEECHHHHHHHHHCCCCHHHHHHHHHCCCCCEECHH
RIDEIERETKHDVIAFLTHLSEIVGPEARFVHQGMTSSDVLDTCLNVQLVRASDILLDDL
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHCEEEEHHHHHHHHHH
DRLLEVLKKRAFEHKDTVTIGRSHGIHAEPTTFGIKLAQAYAEFSRCRERLEHAREEVAT
HHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CAISGAVGTFANIDPRVEEHVAEKMGLKPEPVSTQVIPRDRHAMFFATLAVIASSIERLA
HHHHCCHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TEIRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLSENLTGLARMVRSYAMPAMENVAL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
WHERDISHSSVERMIGPDATVTLDFALVRLTGLMEKLVVYPENMLRNLDRFRGLVHSQRV
HHHCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LLALTQAGVSREDAYRLVQRNAMKVWEHGADFQQELLADKDVRAALSEAEIREKFDLGYH
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCC
TKHVDTIFKRVFGKA
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MIPRYSRPEMVSIWSPETRFRIWFEIEAHAADAMAKLGIVPQEAARTIWEKGGNATFDID
CCCCCCCCCEEEEECCCCEEEEEEEEECHHHHHHHHHCCCCHHHHHHHHHCCCCCEECHH
RIDEIERETKHDVIAFLTHLSEIVGPEARFVHQGMTSSDVLDTCLNVQLVRASDILLDDL
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHCEEEEHHHHHHHHHH
DRLLEVLKKRAFEHKDTVTIGRSHGIHAEPTTFGIKLAQAYAEFSRCRERLEHAREEVAT
HHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CAISGAVGTFANIDPRVEEHVAEKMGLKPEPVSTQVIPRDRHAMFFATLAVIASSIERLA
HHHHCCHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TEIRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLSENLTGLARMVRSYAMPAMENVAL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHH
WHERDISHSSVERMIGPDATVTLDFALVRLTGLMEKLVVYPENMLRNLDRFRGLVHSQRV
HHHCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LLALTQAGVSREDAYRLVQRNAMKVWEHGADFQQELLADKDVRAALSEAEIREKFDLGYH
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCC
TKHVDTIFKRVFGKA
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]