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The map label for this gene is ttcA

Identifier: 110633624

GI number: 110633624

Start: 1366522

End: 1367376

Strand: Direct

Name: ttcA

Synonym: Meso_1271

Alternate gene names: 110633624

Gene position: 1366522-1367376 (Clockwise)

Preceding gene: 110633623

Following gene: 110633627

Centisome position: 30.97

GC content: 59.3

Gene sequence:

>855_bases
ATGACGTTCCATCAGCCAGTGTCTGAAACGGCCCAACCGGATGAGGCTTCCGGCCATCCACTTTTTGCCGATGCTCCCTC
CTCAGTGGAGTTCAACAAGCTCCGCAAGAGGCTTCTGCGCCAGGTGCGCCAGGCGTTCGAGGATTTCACCATGGTGAAGC
CGGGCGACCGCTGGCTGGTGGCGCTTTCGGGCGGCAAGGATTCCTACGGGCTGCTTGCCGTTCTCCTCGATCTCAAATGG
CGCGGTCTCCTGCCGGTCGAGCTTCTGGCCTGCAATCTCGACCAGGGGCAGCCGAATTTTCCCAAGCATATTTTGCCGGA
TTATCTTACCCGTCTCGGCGTTCCCCATCGCATTGAATACCAAGACACCTATTCCGTGGTGACGGAAAAGATTCCTGAAA
ACCGCACCTATTGCTCCCTCTGTTCGCGCCTTCGCCGCGGGCATCTGTACCGCATCGCGCGGGAGGAGGGGTGCTCCTCG
CTCGTTCTCGGCCATCACCGCGAGGACATTCTGGAAACCTTCTTCATGAATTTCTTCCACGGCGGGCGGCTTGCAGCCAT
GCCGCCGAAGCTTCTGAACGACGAGGGCGATGTACTGGTGCTGCGGCCGCTCGCCTATTGCGCGGAGACGGACTTGGTGA
AATTCGCCGAGGGAATGAAATTCCCGATCATTCCCTGCGATCTCTGCGGCAGCCAGGATGGCCTGCAACGCAACGCGATG
AAGGCAATGCTGGAAGATATCGAGCGTCGTATGCCCGGCCGCAAGGATACAATGATCCGCGCGCTCGCCAATGTGCGGCC
GTCGCATCTGCTGGACCGGCAACTCTTCGACTTTGCCGGTCTCGGAATTCTCTAA

Upstream 100 bases:

>100_bases
GTACTATTCGCGCTGATATCGTTCGAATTGCCGGAAAAGGCCGCCAGTGGCGGCCTTTTTCTTTTGGCGGACCGGCGTTA
TGGAGGGGCAGGAGATTGCC

Downstream 100 bases:

>100_bases
ACCGGCGCGCTTACCTGGCGGAACAGCTGTCCGCGTTCGCCGATCAGAACCAGGATCAGGCCGACACCTCCTGAGATGAA
ATAGCCAGCCGCCAGCGGCA

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MTFHQPVSETAQPDEASGHPLFADAPSSVEFNKLRKRLLRQVRQAFEDFTMVKPGDRWLVALSGGKDSYGLLAVLLDLKW
RGLLPVELLACNLDQGQPNFPKHILPDYLTRLGVPHRIEYQDTYSVVTEKIPENRTYCSLCSRLRRGHLYRIAREEGCSS
LVLGHHREDILETFFMNFFHGGRLAAMPPKLLNDEGDVLVLRPLAYCAETDLVKFAEGMKFPIIPCDLCGSQDGLQRNAM
KAMLEDIERRMPGRKDTMIRALANVRPSHLLDRQLFDFAGLGIL

Sequences:

>Translated_284_residues
MTFHQPVSETAQPDEASGHPLFADAPSSVEFNKLRKRLLRQVRQAFEDFTMVKPGDRWLVALSGGKDSYGLLAVLLDLKW
RGLLPVELLACNLDQGQPNFPKHILPDYLTRLGVPHRIEYQDTYSVVTEKIPENRTYCSLCSRLRRGHLYRIAREEGCSS
LVLGHHREDILETFFMNFFHGGRLAAMPPKLLNDEGDVLVLRPLAYCAETDLVKFAEGMKFPIIPCDLCGSQDGLQRNAM
KAMLEDIERRMPGRKDTMIRALANVRPSHLLDRQLFDFAGLGIL
>Mature_283_residues
TFHQPVSETAQPDEASGHPLFADAPSSVEFNKLRKRLLRQVRQAFEDFTMVKPGDRWLVALSGGKDSYGLLAVLLDLKWR
GLLPVELLACNLDQGQPNFPKHILPDYLTRLGVPHRIEYQDTYSVVTEKIPENRTYCSLCSRLRRGHLYRIAREEGCSSL
VLGHHREDILETFFMNFFHGGRLAAMPPKLLNDEGDVLVLRPLAYCAETDLVKFAEGMKFPIIPCDLCGSQDGLQRNAMK
AMLEDIERRMPGRKDTMIRALANVRPSHLLDRQLFDFAGLGIL

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32)

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family

Homologues:

Organism=Escherichia coli, GI1787606, Length=251, Percent_Identity=55.3784860557769, Blast_Score=303, Evalue=7e-84,
Organism=Caenorhabditis elegans, GI193211090, Length=241, Percent_Identity=31.9502074688797, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17540090, Length=184, Percent_Identity=30.9782608695652, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24651972, Length=225, Percent_Identity=26.6666666666667, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TTCA_MESSB (Q11IV8)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_673832.1
- ProteinModelPortal:   Q11IV8
- SMR:   Q11IV8
- STRING:   Q11IV8
- GeneID:   4180569
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_1271
- NMPDR:   fig|266779.1.peg.2317
- eggNOG:   COG0037
- HOGENOM:   HBG347038
- OMA:   EHILPEY
- PhylomeDB:   Q11IV8
- ProtClustDB:   PRK10696
- BioCyc:   MSP266779:MESO_1271-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01850
- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF004976

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 32234; Mature: 32103

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFHQPVSETAQPDEASGHPLFADAPSSVEFNKLRKRLLRQVRQAFEDFTMVKPGDRWLV
CCCCCCCHHCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
ALSGGKDSYGLLAVLLDLKWRGLLPVELLACNLDQGQPNFPKHILPDYLTRLGVPHRIEY
EEECCCCCHHHHHHHHHHCCCCCCCHHHHEECCCCCCCCCCHHHHHHHHHHCCCCCEECH
QDTYSVVTEKIPENRTYCSLCSRLRRGHLYRIAREEGCSSLVLGHHREDILETFFMNFFH
HHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHC
GGRLAAMPPKLLNDEGDVLVLRPLAYCAETDLVKFAEGMKFPIIPCDLCGSQDGLQRNAM
CCEEECCCHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCEEHHHCCCCCHHHHHHH
KAMLEDIERRMPGRKDTMIRALANVRPSHLLDRQLFDFAGLGIL
HHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TFHQPVSETAQPDEASGHPLFADAPSSVEFNKLRKRLLRQVRQAFEDFTMVKPGDRWLV
CCCCCCHHCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
ALSGGKDSYGLLAVLLDLKWRGLLPVELLACNLDQGQPNFPKHILPDYLTRLGVPHRIEY
EEECCCCCHHHHHHHHHHCCCCCCCHHHHEECCCCCCCCCCHHHHHHHHHHCCCCCEECH
QDTYSVVTEKIPENRTYCSLCSRLRRGHLYRIAREEGCSSLVLGHHREDILETFFMNFFH
HHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHC
GGRLAAMPPKLLNDEGDVLVLRPLAYCAETDLVKFAEGMKFPIIPCDLCGSQDGLQRNAM
CCEEECCCHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCEEHHHCCCCCHHHHHHH
KAMLEDIERRMPGRKDTMIRALANVRPSHLLDRQLFDFAGLGIL
HHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA