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The map label for this gene is trmJ [H]

Identifier: 110633621

GI number: 110633621

Start: 1363855

End: 1364643

Strand: Direct

Name: trmJ [H]

Synonym: Meso_1268

Alternate gene names: 110633621

Gene position: 1363855-1364643 (Clockwise)

Preceding gene: 110633619

Following gene: 110633622

Centisome position: 30.91

GC content: 63.12

Gene sequence:

>789_bases
ATGGCAGGCACGGATAGACAGCGCGATCTGCTCGCCGAAGGACCCGCAATCGTCCTCGTCGAGCCTCAGTTGGGTGAGAA
TATCGGCATGGTGGCACGCGCCATGGCCAATTTCGGCTTGGGCGAGCTGCGCCTCGTCAAACCGCGGGACGGCTGGCCGA
ATGAAAAGGCCCGCGCTGCCGCAAGCCGGGCCGATCATGTCATCGACGCGACCAAGGTCTTCGACGATCTTGCGGGGGCG
GTCGCGGACCTGAACTTCCTATATGCGACCACGGCCCGGCCGCGCGACAATTTCAAGCCGGTGCGCGGGCCGGTGGAGGC
AACGCTGCAATTGCGCGCACGTCATGCCGCGGGGCAGAGGACCGGCATTCTCTTCGGCCGCGAGCGCTGGGGGCTCACCA
ATGAAGAGGTTGCGGTTGCCGACGAAATCGTGACATTCCCGGTCAACCCCGCCTTTGCCTCGCTCAATGTCGCCCAGGCG
GTGCTTCTGATGTCCTATGAATGGTTGAAGGCCGGAGGGGAGGGCGTAGCGCCCGTCTCGCCCGAGCACGTTCCCGCGCC
GCGTGAACACCTCAACAGCCTCATGGATTATCTTGACCGCGTGCTCTCGGAGCGCGGCTATTTCCGCAATGAGGACAAGA
AGCCCAAGATGTTCGACAATCTTAGGGCGCTCTTTACCCGGCCGGGTTTTACTGTCGAGGAACTGGCGGTGCTGCGCGGC
GTGCTGGTCTCGCTAGAGAAATTTTCGCCTAAGAAGCCGCGCGGGTCCGGTGCGCCTGAAACCGATTGA

Upstream 100 bases:

>100_bases
ATACTTGAAGGTGGCGCCCCTATAGCATCGTTGCGCCGCTTGGCAAAGACGGCGGATGCCAGGCTTTACCCCGGTGCGAT
AAGGCGCTAACAGCCGCTGC

Downstream 100 bases:

>100_bases
TTCATGATGTCGAGCCCTTTCACGCAACGTAGAGCGCGACGCTCACCGTGGTTCCTCGTGTCACAATGACCGCGCTCGCA
CAAACCAGCCCCATCCTCTT

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MAGTDRQRDLLAEGPAIVLVEPQLGENIGMVARAMANFGLGELRLVKPRDGWPNEKARAAASRADHVIDATKVFDDLAGA
VADLNFLYATTARPRDNFKPVRGPVEATLQLRARHAAGQRTGILFGRERWGLTNEEVAVADEIVTFPVNPAFASLNVAQA
VLLMSYEWLKAGGEGVAPVSPEHVPAPREHLNSLMDYLDRVLSERGYFRNEDKKPKMFDNLRALFTRPGFTVEELAVLRG
VLVSLEKFSPKKPRGSGAPETD

Sequences:

>Translated_262_residues
MAGTDRQRDLLAEGPAIVLVEPQLGENIGMVARAMANFGLGELRLVKPRDGWPNEKARAAASRADHVIDATKVFDDLAGA
VADLNFLYATTARPRDNFKPVRGPVEATLQLRARHAAGQRTGILFGRERWGLTNEEVAVADEIVTFPVNPAFASLNVAQA
VLLMSYEWLKAGGEGVAPVSPEHVPAPREHLNSLMDYLDRVLSERGYFRNEDKKPKMFDNLRALFTRPGFTVEELAVLRG
VLVSLEKFSPKKPRGSGAPETD
>Mature_261_residues
AGTDRQRDLLAEGPAIVLVEPQLGENIGMVARAMANFGLGELRLVKPRDGWPNEKARAAASRADHVIDATKVFDDLAGAV
ADLNFLYATTARPRDNFKPVRGPVEATLQLRARHAAGQRTGILFGRERWGLTNEEVAVADEIVTFPVNPAFASLNVAQAV
LLMSYEWLKAGGEGVAPVSPEHVPAPREHLNSLMDYLDRVLSERGYFRNEDKKPKMFDNLRALFTRPGFTVEELAVLRGV
LVSLEKFSPKKPRGSGAPETD

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=236, Percent_Identity=38.135593220339, Blast_Score=120, Evalue=8e-29,
Organism=Escherichia coli, GI1790865, Length=151, Percent_Identity=38.4105960264901, Blast_Score=99, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28740; Mature: 28609

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGTDRQRDLLAEGPAIVLVEPQLGENIGMVARAMANFGLGELRLVKPRDGWPNEKARAA
CCCCCCHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHH
ASRADHVIDATKVFDDLAGAVADLNFLYATTARPRDNFKPVRGPVEATLQLRARHAAGQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
TGILFGRERWGLTNEEVAVADEIVTFPVNPAFASLNVAQAVLLMSYEWLKAGGEGVAPVS
CEEEEEHHHCCCCCCCHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PEHVPAPREHLNSLMDYLDRVLSERGYFRNEDKKPKMFDNLRALFTRPGFTVEELAVLRG
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHH
VLVSLEKFSPKKPRGSGAPETD
HHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AGTDRQRDLLAEGPAIVLVEPQLGENIGMVARAMANFGLGELRLVKPRDGWPNEKARAA
CCCCCHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHH
ASRADHVIDATKVFDDLAGAVADLNFLYATTARPRDNFKPVRGPVEATLQLRARHAAGQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
TGILFGRERWGLTNEEVAVADEIVTFPVNPAFASLNVAQAVLLMSYEWLKAGGEGVAPVS
CEEEEEHHHCCCCCCCHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PEHVPAPREHLNSLMDYLDRVLSERGYFRNEDKKPKMFDNLRALFTRPGFTVEELAVLRG
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHH
VLVSLEKFSPKKPRGSGAPETD
HHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA