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The map label for this gene is mii [H]

Identifier: 110633402

GI number: 110633402

Start: 1151892

End: 1153103

Strand: Reverse

Name: mii [H]

Synonym: Meso_1048

Alternate gene names: 110633402

Gene position: 1153103-1151892 (Counterclockwise)

Preceding gene: 110633403

Following gene: 110633401

Centisome position: 26.13

GC content: 65.68

Gene sequence:

>1212_bases
ATGGTTGAGCCCAGACCGTGCTCCAAGGAGGAGGTCATGAACAGGATGCAGGAAATCCGCACGCCGGATCAGATCGCGAT
CCCCGCCGTCTTCATGCGCGGCGGCACATCGAAGGCGATCGTCTTCCACGCGCGCGACCTGCCGGAGAGCCGGGAAGCGC
GGGATCGGATTTTCCTGGCAGCAATGGGCTCGCCCGACCCCAATGAGCGGCAGTTGAACGGCATGGGCGGCGGGCTTTCC
TCCCTGTCGAAGGTCTGCATCATCGAGCGTTCCAAGGCTCCGGGCGCCGATATCGACTACACTTTCGGGCAGGTGGAGGT
CCTTGCAGCACGCGTCGACTACAACGGCAGTTGCGGCAGCATGGCTTCCGCCATGGGCCCCTTTGCCATAGACGAAGGGC
TCGTCGAGGCGCCTGCCGATGGCGAGGCGGTCGTGCGCATCCGCGACGTCAACGCCAACAAGATCATTGTTTCGCGGTTT
CCAACGCGCGGCGGCAAGGCGGCCGTCCGTGGAGACTTTGCCGTGGACGGCATTGCCGGCACGGGCGCTCCGGTCAGGCT
GGAGTTCCAGGCGCCAGGCGGAGCGCATACCGGAAGCCTGCTGCCCTTGGGCGGCATCACCGACACGGTTGAGGTGGCCG
GCATCGGCCGGATCGACTTCAGCTTCGTCGATGCGGCCAATCCGGTCATGTTCGTGGATGCGGAGGCGGTTGGGCTCACC
GGGACGGAACTGCCGGAGGAAATCGAAGCCAAACCCGGCTTCCTCGACCGGGTCGAGACGATCCGCCGTCATGTGACGGT
CAAGGCCGGTGCCGCACCCGATCTTGCGGCCGCCGGCGCCGTATCGTTGCCGCGCATCGCCTTCGTCGCGCCGCCCAAAG
CGTATACGGCCCTTTCGGGCCAGCCCTTCCGATCCGACCAGACCGATATTCTTATCCGCATGATCGGCAGGGGCCGGCCA
CACCGCGCCGTTCCGATAACGACGGCGCTTTGCCTTGCCGTCGCCTGCCGCTTGCCTGGCAGCGTGCCGAACAGGATGTT
GAGCGGGCAAGCAAGGGACGGCGGCGATATCATCGTTGGCCATCCCAGCGGAGCATGGTCGATTGCCGCGACGGTTCGGG
AAACGCCGGATGGGCCCTATGCCGAGATGGCGGGTACCATCATGACCGCGCGCCGGCTCTTCCAGGGCGAAGTGCTGGTT
CCGCGAGAATAG

Upstream 100 bases:

>100_bases
CTACGCCAAGCTCTCCAAAGTGCCCTATGATCCAAAGGCGCAATACCCCGAACTCCTGAAAGTGCTGACGGACAAGGGCG
GAACGTGACGTTCACCGTCA

Downstream 100 bases:

>100_bases
CCCGGCTTCGGCGCCCCCAAAGCGCAATGCAGGGGTCCCATTCGTGATGATGCGGTGAATGCGACGGTGAACCTACCGTC
CAGCGGCTGATTGGTCGTCG

Product: methylitaconate delta2-delta3-isomerase

Products: NA

Alternate protein names: (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MVEPRPCSKEEVMNRMQEIRTPDQIAIPAVFMRGGTSKAIVFHARDLPESREARDRIFLAAMGSPDPNERQLNGMGGGLS
SLSKVCIIERSKAPGADIDYTFGQVEVLAARVDYNGSCGSMASAMGPFAIDEGLVEAPADGEAVVRIRDVNANKIIVSRF
PTRGGKAAVRGDFAVDGIAGTGAPVRLEFQAPGGAHTGSLLPLGGITDTVEVAGIGRIDFSFVDAANPVMFVDAEAVGLT
GTELPEEIEAKPGFLDRVETIRRHVTVKAGAAPDLAAAGAVSLPRIAFVAPPKAYTALSGQPFRSDQTDILIRMIGRGRP
HRAVPITTALCLAVACRLPGSVPNRMLSGQARDGGDIIVGHPSGAWSIAATVRETPDGPYAEMAGTIMTARRLFQGEVLV
PRE

Sequences:

>Translated_403_residues
MVEPRPCSKEEVMNRMQEIRTPDQIAIPAVFMRGGTSKAIVFHARDLPESREARDRIFLAAMGSPDPNERQLNGMGGGLS
SLSKVCIIERSKAPGADIDYTFGQVEVLAARVDYNGSCGSMASAMGPFAIDEGLVEAPADGEAVVRIRDVNANKIIVSRF
PTRGGKAAVRGDFAVDGIAGTGAPVRLEFQAPGGAHTGSLLPLGGITDTVEVAGIGRIDFSFVDAANPVMFVDAEAVGLT
GTELPEEIEAKPGFLDRVETIRRHVTVKAGAAPDLAAAGAVSLPRIAFVAPPKAYTALSGQPFRSDQTDILIRMIGRGRP
HRAVPITTALCLAVACRLPGSVPNRMLSGQARDGGDIIVGHPSGAWSIAATVRETPDGPYAEMAGTIMTARRLFQGEVLV
PRE
>Mature_403_residues
MVEPRPCSKEEVMNRMQEIRTPDQIAIPAVFMRGGTSKAIVFHARDLPESREARDRIFLAAMGSPDPNERQLNGMGGGLS
SLSKVCIIERSKAPGADIDYTFGQVEVLAARVDYNGSCGSMASAMGPFAIDEGLVEAPADGEAVVRIRDVNANKIIVSRF
PTRGGKAAVRGDFAVDGIAGTGAPVRLEFQAPGGAHTGSLLPLGGITDTVEVAGIGRIDFSFVDAANPVMFVDAEAVGLT
GTELPEEIEAKPGFLDRVETIRRHVTVKAGAAPDLAAAGAVSLPRIAFVAPPKAYTALSGQPFRSDQTDILIRMIGRGRP
HRAVPITTALCLAVACRLPGSVPNRMLSGQARDGGDIIVGHPSGAWSIAATVRETPDGPYAEMAGTIMTARRLFQGEVLV
PRE

Specific function: Catalyzes the reversible isomerization of (R)-3- methylitaconate to 2,3-dimethylmaleate. Has very low isomerase activity with itaconate [H]

COG id: COG2828

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786985, Length=375, Percent_Identity=35.7333333333333, Blast_Score=199, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007400 [H]

Pfam domain/function: PF04303 PrpF [H]

EC number: =5.3.3.6 [H]

Molecular weight: Translated: 42431; Mature: 42431

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEPRPCSKEEVMNRMQEIRTPDQIAIPAVFMRGGTSKAIVFHARDLPESREARDRIFLA
CCCCCCCCHHHHHHHHHHHCCCCCEECCEEEECCCCCEEEEEEECCCCCCCCCCCEEEEE
AMGSPDPNERQLNGMGGGLSSLSKVCIIERSKAPGADIDYTFGQVEVLAARVDYNGSCGS
ECCCCCCCCCEECCCCCCHHHHHEEEEEECCCCCCCCCEECCCCEEEEEEEECCCCCCCH
MASAMGPFAIDEGLVEAPADGEAVVRIRDVNANKIIVSRFPTRGGKAAVRGDFAVDGIAG
HHHHCCCHHHCCCCEECCCCCCEEEEEEECCCCEEEEEECCCCCCCEEEECCEEECCCCC
TGAPVRLEFQAPGGAHTGSLLPLGGITDTVEVAGIGRIDFSFVDAANPVMFVDAEAVGLT
CCCCEEEEEECCCCCCCCCEEECCCCCCCEEEEECCCCCEEEECCCCCEEEEECCEECCC
GTELPEEIEAKPGFLDRVETIRRHVTVKAGAAPDLAAAGAVSLPRIAFVAPPKAYTALSG
CCCCCHHHCCCCCHHHHHHHHHHHEEEECCCCCCHHHCCCCCCCEEEEECCCCHHEECCC
QPFRSDQTDILIRMIGRGRPHRAVPITTALCLAVACRLPGSVPNRMLSGQARDGGDIIVG
CCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCEEEE
HPSGAWSIAATVRETPDGPYAEMAGTIMTARRLFQGEVLVPRE
CCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEECCCC
>Mature Secondary Structure
MVEPRPCSKEEVMNRMQEIRTPDQIAIPAVFMRGGTSKAIVFHARDLPESREARDRIFLA
CCCCCCCCHHHHHHHHHHHCCCCCEECCEEEECCCCCEEEEEEECCCCCCCCCCCEEEEE
AMGSPDPNERQLNGMGGGLSSLSKVCIIERSKAPGADIDYTFGQVEVLAARVDYNGSCGS
ECCCCCCCCCEECCCCCCHHHHHEEEEEECCCCCCCCCEECCCCEEEEEEEECCCCCCCH
MASAMGPFAIDEGLVEAPADGEAVVRIRDVNANKIIVSRFPTRGGKAAVRGDFAVDGIAG
HHHHCCCHHHCCCCEECCCCCCEEEEEEECCCCEEEEEECCCCCCCEEEECCEEECCCCC
TGAPVRLEFQAPGGAHTGSLLPLGGITDTVEVAGIGRIDFSFVDAANPVMFVDAEAVGLT
CCCCEEEEEECCCCCCCCCEEECCCCCCCEEEEECCCCCEEEECCCCCEEEEECCEECCC
GTELPEEIEAKPGFLDRVETIRRHVTVKAGAAPDLAAAGAVSLPRIAFVAPPKAYTALSG
CCCCCHHHCCCCCHHHHHHHHHHHEEEECCCCCCHHHCCCCCCCEEEEECCCCHHEECCC
QPFRSDQTDILIRMIGRGRPHRAVPITTALCLAVACRLPGSVPNRMLSGQARDGGDIIVG
CCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCEEEE
HPSGAWSIAATVRETPDGPYAEMAGTIMTARRLFQGEVLVPRE
CCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA