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The map label for this gene is gyrB [H]

Identifier: 110633369

GI number: 110633369

Start: 1112216

End: 1114267

Strand: Reverse

Name: gyrB [H]

Synonym: Meso_1015

Alternate gene names: 110633369

Gene position: 1114267-1112216 (Counterclockwise)

Preceding gene: 110633370

Following gene: 110633367

Centisome position: 25.25

GC content: 64.33

Gene sequence:

>2052_bases
ATGGACGACACCAACGACCTCTTTGCAACGCTTGGCTCCTCCAGCGCAGAGCGCGTCAAGCAGCCGGAACCGATTGCGAC
TGCGCGCAAGGCGCAGCGTGCGCCATCCCCTGCGCGCGATGCAAGCGACAGCTACAGCGCGGCCGACATCGAGGTTCTGG
AAGGCCTGGAGCCGGTGCGCCGGCGGCCTGGCATGTATATCGGCGGCACGGACGACAAGGCGCTGCACCACCTCTTTGCC
GAGGTGATCGACAATTCCATGGATGAGGCCGTGGCCGGCCACGCGACCTTCATCGACGTTGAGCTCTCCGAAGACGGATT
CCTCACCGTCACCGACAATGGCCGCGGCATTCCGGTCGACCCGCACCCGAAATTCAAGGACAAGTCCGCCCTGGAAGTCA
TCATGACGACGCTGCATTCGGGCGGCAAATTCGATTCCAAGGTCTATGAGACCTCCGGCGGCCTTCACGGCGTGGGCGTT
TCTGTGGTCAACGCGCTTTCCGAGTTGCTGGAGGTGGAGGTCGCGCGCGGGCGCCAGCTCTTCCGCCAGCGCTTTTCGCG
CGGCAAACCGCTCGGCCCGCTCGAAAGCGTGGGCGAGGTGCATAACCGGCGCGGCACGCGCACACGCTTCAAGCCGGACC
CGGAGATCTTCGGCGCGGGGGCAAAATTCGAGCCGGCGCGGCTCTACCGCATGACGCGCTCAAAGGCGTACCTCTTCGGA
GGTGTCGAGATCCGCTGGAACTGCGCGCCTTCGCTGCTCAAGGAGAAGGACGAAACGCCGGACAAGGCCGTCTTCCATTT
CCCCGGCGGCCTGAAGGACTATCTTTCAAACTCCATCGACGGCGAATTCCAAGTCACCCGCGAAGTCTTCGCCGGCAAGA
CCGAGAAGCAAAGCGGCCACGGCGCAGTGGAGTGGGCGGTCACCTGGTATGGCGGCGACGCTTTCCTCAATTCCTACTGC
AACACCATCCCGACGCCCGAGGGCGGCACGCATGAACAGGGCTTCCGAAACGCGCTCCTGAGGGGCCTGCGCGGCTACGC
CGACCTCACGGGCAACAAGCGCGCCTCCATCGTCACCGCCGAGGACGTGATGATCTCGGCCGCCGGCATGCTCTCGGTCT
TCGTGCGCGAGCCCGAATTCGTCGGCCAGACGAAGGACAAGCTCGCCACCGCCGCAGCCATGCGCATTGTCGAAAACGCG
GTGCGCGATCCCTTCGACCATTGGCTGGCGGACAACCCGGCCGAGGCTTCCAAGCTGCTCGACTGGGTGATCGCCCGCGC
CGACGAACGGCTGAAACGCCGGCAGGAAAAGGAAGTCAGCCGCAAATCGGCGGTGCGCAAGCTGCGCCTGCCCGGCAAGC
TCGCCGATTGTACCGTGGGCGCTGCCGCGGGTGCCGAGATCTTCATCGTCGAAGGTGATTCGGCCGGCGGCTCGGCCAAG
CAGGCGCGTAACCGCGCCACACAGGCGGTGCTGCCTTTGCGCGGCAAGATCCTCAACGTGGCCAGCGCGGGCTCTGGCAA
GCTCGCCGCCAACCAGCAGATCACCGACCTGATCCAGGCACTTGGCTGCGGCACACGATCGAAATACCGCGAGGAGGATC
TGCGCTACGAGCGCGTGATCATCATGACGGATGCCGACGTGGACGGCGCGCACATTGCCTCCCTCCTCATCACCTTCTTC
TATCAGGAGATGCCGCAGCTCATCACCGGCGGGCACCTGTTCCTGGCCGTGCCGCCGCTCTACCGCATCAGCCAGGGCGG
CAAGACCATGTATGCCCGCGACGACGCCCATAAGGACGAGTTGCTGAAAACCGAGTTCACCGGCCGCGGCAAGGTAGAGA
TCGGCCGCTTCAAGGGTCTCGGCGAGATGATGCCGGCGCAGCTCAAGGAAACGACCATGGATCCGAAATACCGCACCCTT
CTGCGCGTTGAGGTCCTGGACACGCCGGAAGCCACGAGCAGCGCTGTCGATGCATTGATGGGCACCAAGCCGGAAGCCCG
CTTCCGCTTCATCCAGGAAAACGCGGAATTCGTGGAAGAGCTGGATATTTGA

Upstream 100 bases:

>100_bases
TCCACAACCACCGAACGGAACCGGAACGGCCTTCGCTTTGCCATGGAAACCATGGCAAACTGGATGTGATTCGCGGGGTG
CGCAGGGGAAAAGAATTTCG

Downstream 100 bases:

>100_bases
AGAGCGAATAGGGAATAGCGAGTAGCGAATAGGGGAGTTAGTGAATAGCGTCGCGGCAGCGACAGCGGCGACTTCCTGTC
TCCTGCTCTTCTATTCGCTA

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 683; Mature: 683

Protein sequence:

>683_residues
MDDTNDLFATLGSSSAERVKQPEPIATARKAQRAPSPARDASDSYSAADIEVLEGLEPVRRRPGMYIGGTDDKALHHLFA
EVIDNSMDEAVAGHATFIDVELSEDGFLTVTDNGRGIPVDPHPKFKDKSALEVIMTTLHSGGKFDSKVYETSGGLHGVGV
SVVNALSELLEVEVARGRQLFRQRFSRGKPLGPLESVGEVHNRRGTRTRFKPDPEIFGAGAKFEPARLYRMTRSKAYLFG
GVEIRWNCAPSLLKEKDETPDKAVFHFPGGLKDYLSNSIDGEFQVTREVFAGKTEKQSGHGAVEWAVTWYGGDAFLNSYC
NTIPTPEGGTHEQGFRNALLRGLRGYADLTGNKRASIVTAEDVMISAAGMLSVFVREPEFVGQTKDKLATAAAMRIVENA
VRDPFDHWLADNPAEASKLLDWVIARADERLKRRQEKEVSRKSAVRKLRLPGKLADCTVGAAAGAEIFIVEGDSAGGSAK
QARNRATQAVLPLRGKILNVASAGSGKLAANQQITDLIQALGCGTRSKYREEDLRYERVIIMTDADVDGAHIASLLITFF
YQEMPQLITGGHLFLAVPPLYRISQGGKTMYARDDAHKDELLKTEFTGRGKVEIGRFKGLGEMMPAQLKETTMDPKYRTL
LRVEVLDTPEATSSAVDALMGTKPEARFRFIQENAEFVEELDI

Sequences:

>Translated_683_residues
MDDTNDLFATLGSSSAERVKQPEPIATARKAQRAPSPARDASDSYSAADIEVLEGLEPVRRRPGMYIGGTDDKALHHLFA
EVIDNSMDEAVAGHATFIDVELSEDGFLTVTDNGRGIPVDPHPKFKDKSALEVIMTTLHSGGKFDSKVYETSGGLHGVGV
SVVNALSELLEVEVARGRQLFRQRFSRGKPLGPLESVGEVHNRRGTRTRFKPDPEIFGAGAKFEPARLYRMTRSKAYLFG
GVEIRWNCAPSLLKEKDETPDKAVFHFPGGLKDYLSNSIDGEFQVTREVFAGKTEKQSGHGAVEWAVTWYGGDAFLNSYC
NTIPTPEGGTHEQGFRNALLRGLRGYADLTGNKRASIVTAEDVMISAAGMLSVFVREPEFVGQTKDKLATAAAMRIVENA
VRDPFDHWLADNPAEASKLLDWVIARADERLKRRQEKEVSRKSAVRKLRLPGKLADCTVGAAAGAEIFIVEGDSAGGSAK
QARNRATQAVLPLRGKILNVASAGSGKLAANQQITDLIQALGCGTRSKYREEDLRYERVIIMTDADVDGAHIASLLITFF
YQEMPQLITGGHLFLAVPPLYRISQGGKTMYARDDAHKDELLKTEFTGRGKVEIGRFKGLGEMMPAQLKETTMDPKYRTL
LRVEVLDTPEATSSAVDALMGTKPEARFRFIQENAEFVEELDI
>Mature_683_residues
MDDTNDLFATLGSSSAERVKQPEPIATARKAQRAPSPARDASDSYSAADIEVLEGLEPVRRRPGMYIGGTDDKALHHLFA
EVIDNSMDEAVAGHATFIDVELSEDGFLTVTDNGRGIPVDPHPKFKDKSALEVIMTTLHSGGKFDSKVYETSGGLHGVGV
SVVNALSELLEVEVARGRQLFRQRFSRGKPLGPLESVGEVHNRRGTRTRFKPDPEIFGAGAKFEPARLYRMTRSKAYLFG
GVEIRWNCAPSLLKEKDETPDKAVFHFPGGLKDYLSNSIDGEFQVTREVFAGKTEKQSGHGAVEWAVTWYGGDAFLNSYC
NTIPTPEGGTHEQGFRNALLRGLRGYADLTGNKRASIVTAEDVMISAAGMLSVFVREPEFVGQTKDKLATAAAMRIVENA
VRDPFDHWLADNPAEASKLLDWVIARADERLKRRQEKEVSRKSAVRKLRLPGKLADCTVGAAAGAEIFIVEGDSAGGSAK
QARNRATQAVLPLRGKILNVASAGSGKLAANQQITDLIQALGCGTRSKYREEDLRYERVIIMTDADVDGAHIASLLITFF
YQEMPQLITGGHLFLAVPPLYRISQGGKTMYARDDAHKDELLKTEFTGRGKVEIGRFKGLGEMMPAQLKETTMDPKYRTL
LRVEVLDTPEATSSAVDALMGTKPEARFRFIQENAEFVEELDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=588, Percent_Identity=24.4897959183673, Blast_Score=126, Evalue=6e-29,
Organism=Homo sapiens, GI19913408, Length=660, Percent_Identity=23.7878787878788, Blast_Score=122, Evalue=8e-28,
Organism=Escherichia coli, GI1789408, Length=649, Percent_Identity=42.2187981510015, Blast_Score=456, Evalue=1e-129,
Organism=Escherichia coli, GI48994957, Length=567, Percent_Identity=44.6208112874779, Blast_Score=434, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI212645845, Length=575, Percent_Identity=23.6521739130435, Blast_Score=119, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17535065, Length=587, Percent_Identity=22.4872231686542, Blast_Score=99, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6324241, Length=637, Percent_Identity=24.8037676609105, Blast_Score=107, Evalue=4e-24,
Organism=Drosophila melanogaster, GI17136538, Length=600, Percent_Identity=24.6666666666667, Blast_Score=114, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 74896; Mature: 74896

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDTNDLFATLGSSSAERVKQPEPIATARKAQRAPSPARDASDSYSAADIEVLEGLEPVR
CCCCHHHHHHHCCCCHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCHHHH
RRPGMYIGGTDDKALHHLFAEVIDNSMDEAVAGHATFIDVELSEDGFLTVTDNGRGIPVD
HCCCEEECCCCHHHHHHHHHHHHHCHHHHHHCCCEEEEEEEECCCCEEEECCCCCCCCCC
PHPKFKDKSALEVIMTTLHSGGKFDSKVYETSGGLHGVGVSVVNALSELLEVEVARGRQL
CCCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FRQRFSRGKPLGPLESVGEVHNRRGTRTRFKPDPEIFGAGAKFEPARLYRMTRSKAYLFG
HHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHCCEEEEE
GVEIRWNCAPSLLKEKDETPDKAVFHFPGGLKDYLSNSIDGEFQVTREVFAGKTEKQSGH
CEEEEECCCHHHHHCCCCCCCCEEEECCCCHHHHHCCCCCCCHHHHHHHHCCCCCCCCCC
GAVEWAVTWYGGDAFLNSYCNTIPTPEGGTHEQGFRNALLRGLRGYADLTGNKRASIVTA
CEEEEEEEEECCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEH
EDVMISAAGMLSVFVREPEFVGQTKDKLATAAAMRIVENAVRDPFDHWLADNPAEASKLL
HHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHH
DWVIARADERLKRRQEKEVSRKSAVRKLRLPGKLADCTVGAAAGAEIFIVEGDSAGGSAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCEEEEEECCCCCCCHH
QARNRATQAVLPLRGKILNVASAGSGKLAANQQITDLIQALGCGTRSKYREEDLRYERVI
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHCCCEEEEE
IMTDADVDGAHIASLLITFFYQEMPQLITGGHLFLAVPPLYRISQGGKTMYARDDAHKDE
EEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHCCCCCCEEEECCCCCHHH
LLKTEFTGRGKVEIGRFKGLGEMMPAQLKETTMDPKYRTLLRVEVLDTPEATSSAVDALM
HHHHHCCCCCCEEEHHHCCCHHHHHHHHHHHCCCCCHHEEEEEEEECCCCHHHHHHHHHH
GTKPEARFRFIQENAEFVEELDI
CCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDDTNDLFATLGSSSAERVKQPEPIATARKAQRAPSPARDASDSYSAADIEVLEGLEPVR
CCCCHHHHHHHCCCCHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCHHHH
RRPGMYIGGTDDKALHHLFAEVIDNSMDEAVAGHATFIDVELSEDGFLTVTDNGRGIPVD
HCCCEEECCCCHHHHHHHHHHHHHCHHHHHHCCCEEEEEEEECCCCEEEECCCCCCCCCC
PHPKFKDKSALEVIMTTLHSGGKFDSKVYETSGGLHGVGVSVVNALSELLEVEVARGRQL
CCCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FRQRFSRGKPLGPLESVGEVHNRRGTRTRFKPDPEIFGAGAKFEPARLYRMTRSKAYLFG
HHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHCCEEEEE
GVEIRWNCAPSLLKEKDETPDKAVFHFPGGLKDYLSNSIDGEFQVTREVFAGKTEKQSGH
CEEEEECCCHHHHHCCCCCCCCEEEECCCCHHHHHCCCCCCCHHHHHHHHCCCCCCCCCC
GAVEWAVTWYGGDAFLNSYCNTIPTPEGGTHEQGFRNALLRGLRGYADLTGNKRASIVTA
CEEEEEEEEECCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEH
EDVMISAAGMLSVFVREPEFVGQTKDKLATAAAMRIVENAVRDPFDHWLADNPAEASKLL
HHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHH
DWVIARADERLKRRQEKEVSRKSAVRKLRLPGKLADCTVGAAAGAEIFIVEGDSAGGSAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCEEEEEECCCCCCCHH
QARNRATQAVLPLRGKILNVASAGSGKLAANQQITDLIQALGCGTRSKYREEDLRYERVI
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHCCCEEEEE
IMTDADVDGAHIASLLITFFYQEMPQLITGGHLFLAVPPLYRISQGGKTMYARDDAHKDE
EEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHCCCCCCEEEECCCCCHHH
LLKTEFTGRGKVEIGRFKGLGEMMPAQLKETTMDPKYRTLLRVEVLDTPEATSSAVDALM
HHHHHCCCCCCEEEHHHCCCHHHHHHHHHHHCCCCCHHEEEEEEEECCCCHHHHHHHHHH
GTKPEARFRFIQENAEFVEELDI
CCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9797224 [H]