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The map label for this gene is celE [H]

Identifier: 110633266

GI number: 110633266

Start: 1010432

End: 1011532

Strand: Direct

Name: celE [H]

Synonym: Meso_0912

Alternate gene names: 110633266

Gene position: 1010432-1011532 (Clockwise)

Preceding gene: 110633265

Following gene: 110633267

Centisome position: 22.9

GC content: 62.76

Gene sequence:

>1101_bases
ATGGGAATCTCCAGCGTCAGGAAACTGCCCGTTGCCGGCCGCCTTCGAGCTGTCGTTGCGGCGTTTGCCGCCGCGGCATT
CTTCCTTGGCTCTTTTCCTTCCGGCGCTCTCGCCCAGCAAAAGCTGGCCGAGCAGTTTCGCCTTGTCGGCCGGTTCGTCC
AGCCCTCGCCCTCGGCCGCGATCTTCGCCTGGCCGGGCAGCGCGATCGAAATCGCATTCACCGGTTCGCGGCTCGACATC
TCCTTGCGCGATACCGGGCAGAACAGCATGGTAGTCGAACTCGACGGCAAGCCTTCGCGGCTCGATCTCAGGCAAGGAAA
GCACGACTACCGAGTGGTGGACGGTGCTCCGCATGGGCCGCACCTCCTGCGGCTCATCCGACGAACGGAGCCGATGTTCG
GGCCTACTGTCTTTATGGGCGCTGAGACTGACGGCTCCTTTGCCAGGCCTCATGAAAAGGAGAAAAGCCTTTTCGTGATT
GGCGATTCCATATCCGCCGGATATGGCGTGGAGGGCGAGACGACCTCGTGCAAGTTCAGCGCCGATACCGAAAACCAATA
CCTGACCTATGCCGCACTGGTCGCGCGCGCTTTCGAGGCGGATGTGATAACCGCCGCCGTTTCCGGCAAGGGACTCGTCC
GCAACTATGACGGGGGCGCGAAGAACACCATGCCCGAAATCTATCTGCGCGGGCTGCCCGACAGGCCGGGCGATCTTCCG
TTTCCCCGGTCCGATGTGATTATCGTCCATCTCGGCACAAATGATTTCGGCAACGGTGCCCGTCCGCCCGGTTTCGAGGA
GCGCTACGCGGCCTTTCTGGAGGAGCTCAGAAAAAAGGCTCCCGACGCGATGATCTATGCAGCAATGGGGCCATTGCTCT
TCGGCGAGGATCTTAAAGCAGCAGCGGGGGCGGTGAAGCGGGCGGTGGAGGCGCGTGCGGGCGCCGGCGACGACAAGCTC
TCCTTCATCGCGTTCGAGGACCCTGAGGGCAGGGAAGTGCGGGGCTGCGACTGGCATCCGAACGCTGCCGGACAGGAGCA
TATGGCCGATATCCTGGGTGCGAGGCTGGAGGCGGATCTCGGCTGGGAGCGGAGAGAATGA

Upstream 100 bases:

>100_bases
CGCGCGCCTTCACGATCTCTGCTGCAGCGCAAAAAAAGTTATTTTGCGCGAGCGAGGGAAACTCACTTTAGTGGTCGCCG
CAATCTGGGATGCAGCGACC

Downstream 100 bases:

>100_bases
GATCCATGCGGCGAGGTCCTCAAGACGCTCCCGCAGCGGATGCGGTATTTGTCCCGCCGGAGAAAGCCCGGTTGGCGAGT
GAAAACAGGGGTGGCAGGAC

Product: lipolytic enzyme, G-D-S-L

Products: NA

Alternate protein names: Cellulase E; Endo-1,4-beta-glucanase E; EgE [H]

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MGISSVRKLPVAGRLRAVVAAFAAAAFFLGSFPSGALAQQKLAEQFRLVGRFVQPSPSAAIFAWPGSAIEIAFTGSRLDI
SLRDTGQNSMVVELDGKPSRLDLRQGKHDYRVVDGAPHGPHLLRLIRRTEPMFGPTVFMGAETDGSFARPHEKEKSLFVI
GDSISAGYGVEGETTSCKFSADTENQYLTYAALVARAFEADVITAAVSGKGLVRNYDGGAKNTMPEIYLRGLPDRPGDLP
FPRSDVIIVHLGTNDFGNGARPPGFEERYAAFLEELRKKAPDAMIYAAMGPLLFGEDLKAAAGAVKRAVEARAGAGDDKL
SFIAFEDPEGREVRGCDWHPNAAGQEHMADILGARLEADLGWERRE

Sequences:

>Translated_366_residues
MGISSVRKLPVAGRLRAVVAAFAAAAFFLGSFPSGALAQQKLAEQFRLVGRFVQPSPSAAIFAWPGSAIEIAFTGSRLDI
SLRDTGQNSMVVELDGKPSRLDLRQGKHDYRVVDGAPHGPHLLRLIRRTEPMFGPTVFMGAETDGSFARPHEKEKSLFVI
GDSISAGYGVEGETTSCKFSADTENQYLTYAALVARAFEADVITAAVSGKGLVRNYDGGAKNTMPEIYLRGLPDRPGDLP
FPRSDVIIVHLGTNDFGNGARPPGFEERYAAFLEELRKKAPDAMIYAAMGPLLFGEDLKAAAGAVKRAVEARAGAGDDKL
SFIAFEDPEGREVRGCDWHPNAAGQEHMADILGARLEADLGWERRE
>Mature_365_residues
GISSVRKLPVAGRLRAVVAAFAAAAFFLGSFPSGALAQQKLAEQFRLVGRFVQPSPSAAIFAWPGSAIEIAFTGSRLDIS
LRDTGQNSMVVELDGKPSRLDLRQGKHDYRVVDGAPHGPHLLRLIRRTEPMFGPTVFMGAETDGSFARPHEKEKSLFVIG
DSISAGYGVEGETTSCKFSADTENQYLTYAALVARAFEADVITAAVSGKGLVRNYDGGAKNTMPEIYLRGLPDRPGDLPF
PRSDVIIVHLGTNDFGNGARPPGFEERYAAFLEELRKKAPDAMIYAAMGPLLFGEDLKAAAGAVKRAVEARAGAGDDKLS
FIAFEDPEGREVRGCDWHPNAAGQEHMADILGARLEADLGWERRE

Specific function: This enzyme catalyzes the endohydrolysis of 1,4-beta- glucosidic linkages in cellulose, lichenin and cereal beta-D- glucans [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 5 (cellulase A) family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016134
- InterPro:   IPR002105
- InterPro:   IPR018242
- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001547
- InterPro:   IPR018087
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR001087 [H]

Pfam domain/function: PF00150 Cellulase; PF00404 Dockerin_1; PF00657 Lipase_GDSL [H]

EC number: =3.2.1.4 [H]

Molecular weight: Translated: 39468; Mature: 39337

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS01098 LIPASE_GDSL_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGISSVRKLPVAGRLRAVVAAFAAAAFFLGSFPSGALAQQKLAEQFRLVGRFVQPSPSAA
CCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEE
IFAWPGSAIEIAFTGSRLDISLRDTGQNSMVVELDGKPSRLDLRQGKHDYRVVDGAPHGP
EEECCCCEEEEEEECCEEEEEEEECCCCCEEEEECCCCCEEHHHCCCCCEEEECCCCCCH
HLLRLIRRTEPMFGPTVFMGAETDGSFARPHEKEKSLFVIGDSISAGYGVEGETTSCKFS
HHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEE
ADTENQYLTYAALVARAFEADVITAAVSGKGLVRNYDGGAKNTMPEIYLRGLPDRPGDLP
CCCCCCHHHHHHHHHHHHHHHEEEEEECCCCEEECCCCCCCCCCCHHHEECCCCCCCCCC
FPRSDVIIVHLGTNDFGNGARPPGFEERYAAFLEELRKKAPDAMIYAAMGPLLFGEDLKA
CCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEHHCCHHCCCHHHH
AAGAVKRAVEARAGAGDDKLSFIAFEDPEGREVRGCDWHPNAAGQEHMADILGARLEADL
HHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
GWERRE
CCCCCC
>Mature Secondary Structure 
GISSVRKLPVAGRLRAVVAAFAAAAFFLGSFPSGALAQQKLAEQFRLVGRFVQPSPSAA
CCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEE
IFAWPGSAIEIAFTGSRLDISLRDTGQNSMVVELDGKPSRLDLRQGKHDYRVVDGAPHGP
EEECCCCEEEEEEECCEEEEEEEECCCCCEEEEECCCCCEEHHHCCCCCEEEECCCCCCH
HLLRLIRRTEPMFGPTVFMGAETDGSFARPHEKEKSLFVIGDSISAGYGVEGETTSCKFS
HHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEE
ADTENQYLTYAALVARAFEADVITAAVSGKGLVRNYDGGAKNTMPEIYLRGLPDRPGDLP
CCCCCCHHHHHHHHHHHHHHHEEEEEECCCCEEECCCCCCCCCCCHHHEECCCCCCCCCC
FPRSDVIIVHLGTNDFGNGARPPGFEERYAAFLEELRKKAPDAMIYAAMGPLLFGEDLKA
CCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEHHCCHHCCCHHHH
AAGAVKRAVEARAGAGDDKLSFIAFEDPEGREVRGCDWHPNAAGQEHMADILGARLEADL
HHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
GWERRE
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3066698 [H]