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The map label for this gene is mrpD [H]

Identifier: 110633224

GI number: 110633224

Start: 962679

End: 964238

Strand: Direct

Name: mrpD [H]

Synonym: Meso_0867

Alternate gene names: 110633224

Gene position: 962679-964238 (Clockwise)

Preceding gene: 110633223

Following gene: 110633225

Centisome position: 21.82

GC content: 62.88

Gene sequence:

>1560_bases
ATGGCCGTTCCCGGAACCGAAGCCGTCGACCTTTCGGGCGCCATGGTCCTGGCAGCCACGCCGTCGGCCGACTGGCTGGT
GGTGGCGCCTGTGGCGATCCCGATCGTCTTCGGCGCGGTGCTTTTGATGTTCCGCCACGAGACGCGGATTCAGGCCAGCG
TGGCCATCATCGGGCTCATCGCCACGCTCGCCTGCAACCTGCTTCTGCTTTTGCGCATCATGGACCAAGGTCCCGTGGTG
ATGACGATGGGGCGCTGGTTGCCGCCCTTCGGCATCTCTTTCACTGCCGATGTGCTGGGCGCCTCACTGGCACTGGTGGC
CAGCCTGGTCGCGCTCGTCTGCGCGTTCTACTCCACCGCCGATATCGGCACGATCGGGCGGCGCTACGGCTTCTATCCCT
TCCTGATGCTCATGATGGCGGGTGTCGGCGGCTCTTTCCTTACCGGCGATCTTTTCAACCTTTATGTGTGGTTTGAGGTT
TTCCTGATCTCGTCCTTCGGGCTGCTGATCCTCGGATCGGAACACCGGCAGCTGGACGGCGCCACGAAATATGCCATTTT
GAACCTGATCGGCACCACATTCTTCCTGACCGCAACGGCGCTGCTCTATGGCGCGTTCGGCACGCTCAACATGGCTGACA
TCGCGCGCAAGGCAGACAGTGTGCGGGAGGTCGCGCCGCTCACAACGCTTGCGATGTTCTTCCTGCTAGCCTTCGGCATG
AAGGCGGCCGCCTTTCCGGTAAATTTCTGGCTGCCTGCCTCTTATCACACGCCGCGCATCGTTACGGCGGCGCTCTTCGG
CGGCGTTCTGACGAAGGTGGGCGTTTATGCGCTGTTGCGCGTTCTGGTGATGCTCCTGCCGCCGGAGCGCGCGCTCCTTT
CGGGCGTGATCGGCTGGGTGGCGGCGGCCACGATGATCCTGGGAGTCATGGGTGCGCTGGCGCAGACGGATATTCGCCGC
GCCCTGGGCTTTGTGGTGATCTCCAGCGTTGGCATCATGCTTGCCGGCCTTGCGCTAGGCAATGGCGAGGGGCTCGCCGG
AACCATCTTCTACGCGTTTCATTCCATGATCGTCATGGCGGCTCTTTATTTGCTCGCCGGCATCATGAACGGGCTCGGCG
GCAGTTATTCCCTACGCACGCTATCGGGCCTATACGCCAATCACCCGTTGATCGCTGCCTTCGCGCTGATCCTGATCCTC
GCCTCGGCGGGTCTGCCGCCGGCGTCGGGACTTTGGCCGAAGGTGATGCTGGTTCGGGCCTCTCTGGATGCGGGACAGGG
ATGGCTGGCGCTGGCCGTGCTCGCCACCGGCTTCTTCACCACACTGGCGCTCGGCAGGGTTTTCATCCTTGCCGTCTGGC
GGCAGCGCGATGAAGCGGTGACGGCGGAAAGATATTCTCCGCTGCTCGGCTATGTAACGCTGGGCATCCTCGCCGTGCCT
GTGCTTTTCATGGGCCTTTACCCGCAGCCTGTCATCAATATCGCCGAGCAGGCCGCTGGGGGCATTCTCGATGCTTCGAC
TTACATCGGCACGGTGTTTCCGGAGGGGGCGCAGCCATGA

Upstream 100 bases:

>100_bases
GCCTTCCGCACTTACCAGGAACTCGGCACCGACGACACCGACGGTATGCGGGTGGCCGAGCCGAAGGGCGAAGAGCTTCC
ACCGCTGGGGTACTGATCGC

Downstream 100 bases:

>100_bases
CGCTCTACCTGATCAACATCCTGCTCGCGCTCGTCTGGACAGCAATCACCGGTTCCTTCTCGCTTGCCAATATGGTGCTT
GGTTTCCTGCTGGGCTCCTT

Product: putative monovalent cation/H+ antiporter subunit D

Products: NAD+; ubiquinol [C]

Alternate protein names: Mrp complex subunit D; Multiple resistance and pH homeostasis protein D [H]

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MAVPGTEAVDLSGAMVLAATPSADWLVVAPVAIPIVFGAVLLMFRHETRIQASVAIIGLIATLACNLLLLLRIMDQGPVV
MTMGRWLPPFGISFTADVLGASLALVASLVALVCAFYSTADIGTIGRRYGFYPFLMLMMAGVGGSFLTGDLFNLYVWFEV
FLISSFGLLILGSEHRQLDGATKYAILNLIGTTFFLTATALLYGAFGTLNMADIARKADSVREVAPLTTLAMFFLLAFGM
KAAAFPVNFWLPASYHTPRIVTAALFGGVLTKVGVYALLRVLVMLLPPERALLSGVIGWVAAATMILGVMGALAQTDIRR
ALGFVVISSVGIMLAGLALGNGEGLAGTIFYAFHSMIVMAALYLLAGIMNGLGGSYSLRTLSGLYANHPLIAAFALILIL
ASAGLPPASGLWPKVMLVRASLDAGQGWLALAVLATGFFTTLALGRVFILAVWRQRDEAVTAERYSPLLGYVTLGILAVP
VLFMGLYPQPVINIAEQAAGGILDASTYIGTVFPEGAQP

Sequences:

>Translated_519_residues
MAVPGTEAVDLSGAMVLAATPSADWLVVAPVAIPIVFGAVLLMFRHETRIQASVAIIGLIATLACNLLLLLRIMDQGPVV
MTMGRWLPPFGISFTADVLGASLALVASLVALVCAFYSTADIGTIGRRYGFYPFLMLMMAGVGGSFLTGDLFNLYVWFEV
FLISSFGLLILGSEHRQLDGATKYAILNLIGTTFFLTATALLYGAFGTLNMADIARKADSVREVAPLTTLAMFFLLAFGM
KAAAFPVNFWLPASYHTPRIVTAALFGGVLTKVGVYALLRVLVMLLPPERALLSGVIGWVAAATMILGVMGALAQTDIRR
ALGFVVISSVGIMLAGLALGNGEGLAGTIFYAFHSMIVMAALYLLAGIMNGLGGSYSLRTLSGLYANHPLIAAFALILIL
ASAGLPPASGLWPKVMLVRASLDAGQGWLALAVLATGFFTTLALGRVFILAVWRQRDEAVTAERYSPLLGYVTLGILAVP
VLFMGLYPQPVINIAEQAAGGILDASTYIGTVFPEGAQP
>Mature_518_residues
AVPGTEAVDLSGAMVLAATPSADWLVVAPVAIPIVFGAVLLMFRHETRIQASVAIIGLIATLACNLLLLLRIMDQGPVVM
TMGRWLPPFGISFTADVLGASLALVASLVALVCAFYSTADIGTIGRRYGFYPFLMLMMAGVGGSFLTGDLFNLYVWFEVF
LISSFGLLILGSEHRQLDGATKYAILNLIGTTFFLTATALLYGAFGTLNMADIARKADSVREVAPLTTLAMFFLLAFGMK
AAAFPVNFWLPASYHTPRIVTAALFGGVLTKVGVYALLRVLVMLLPPERALLSGVIGWVAAATMILGVMGALAQTDIRRA
LGFVVISSVGIMLAGLALGNGEGLAGTIFYAFHSMIVMAALYLLAGIMNGLGGSYSLRTLSGLYANHPLIAAFALILILA
SAGLPPASGLWPKVMLVRASLDAGQGWLALAVLATGFFTTLALGRVFILAVWRQRDEAVTAERYSPLLGYVTLGILAVPV
LFMGLYPQPVINIAEQAAGGILDASTYIGTVFPEGAQP

Specific function: Mrp complex is a Na(+)/H(+) antiporter involved in Na(+) excretion and Na(+) resistance [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Escherichia coli, GI145693160, Length=476, Percent_Identity=27.7310924369748, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788613, Length=499, Percent_Identity=24.248496993988, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788827, Length=358, Percent_Identity=28.2122905027933, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788829, Length=331, Percent_Identity=25.0755287009063, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788831, Length=401, Percent_Identity=24.9376558603491, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788614, Length=449, Percent_Identity=26.5033407572383, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 54684; Mature: 54553

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVPGTEAVDLSGAMVLAATPSADWLVVAPVAIPIVFGAVLLMFRHETRIQASVAIIGLI
CCCCCCCCEECCCCEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATLACNLLLLLRIMDQGPVVMTMGRWLPPFGISFTADVLGASLALVASLVALVCAFYSTA
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
DIGTIGRRYGFYPFLMLMMAGVGGSFLTGDLFNLYVWFEVFLISSFGLLILGSEHRQLDG
CHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHCCC
ATKYAILNLIGTTFFLTATALLYGAFGTLNMADIARKADSVREVAPLTTLAMFFLLAFGM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KAAAFPVNFWLPASYHTPRIVTAALFGGVLTKVGVYALLRVLVMLLPPERALLSGVIGWV
HHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
AAATMILGVMGALAQTDIRRALGFVVISSVGIMLAGLALGNGEGLAGTIFYAFHSMIVMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
ALYLLAGIMNGLGGSYSLRTLSGLYANHPLIAAFALILILASAGLPPASGLWPKVMLVRA
HHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHEEE
SLDAGQGWLALAVLATGFFTTLALGRVFILAVWRQRDEAVTAERYSPLLGYVTLGILAVP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLFMGLYPQPVINIAEQAAGGILDASTYIGTVFPEGAQP
HHHHCCCCCHHHHHHHHHCCCCEECHHHHHCCCCCCCCC
>Mature Secondary Structure 
AVPGTEAVDLSGAMVLAATPSADWLVVAPVAIPIVFGAVLLMFRHETRIQASVAIIGLI
CCCCCCCEECCCCEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATLACNLLLLLRIMDQGPVVMTMGRWLPPFGISFTADVLGASLALVASLVALVCAFYSTA
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
DIGTIGRRYGFYPFLMLMMAGVGGSFLTGDLFNLYVWFEVFLISSFGLLILGSEHRQLDG
CHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHCCC
ATKYAILNLIGTTFFLTATALLYGAFGTLNMADIARKADSVREVAPLTTLAMFFLLAFGM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KAAAFPVNFWLPASYHTPRIVTAALFGGVLTKVGVYALLRVLVMLLPPERALLSGVIGWV
HHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
AAATMILGVMGALAQTDIRRALGFVVISSVGIMLAGLALGNGEGLAGTIFYAFHSMIVMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
ALYLLAGIMNGLGGSYSLRTLSGLYANHPLIAAFALILILASAGLPPASGLWPKVMLVRA
HHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHEEE
SLDAGQGWLALAVLATGFFTTLALGRVFILAVWRQRDEAVTAERYSPLLGYVTLGILAVP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLFMGLYPQPVINIAEQAAGGILDASTYIGTVFPEGAQP
HHHHCCCCCHHHHHHHHHCCCCEECHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11356194 [H]