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The map label for this gene is comE [H]

Identifier: 110633160

GI number: 110633160

Start: 891998

End: 892567

Strand: Direct

Name: comE [H]

Synonym: Meso_0803

Alternate gene names: 110633160

Gene position: 891998-892567 (Clockwise)

Preceding gene: 110633159

Following gene: 110633161

Centisome position: 20.22

GC content: 62.46

Gene sequence:

>570_bases
ATGATGAAACGTGATCAGGTCCTCAAAATCCTCGCGCGTCATCTCGATCGGGAGATCGTCATCGGCGCATACAGCACCGC
CTTCGATTGGATCGCCACGCGTCCGGGCCATCCTCTGAATTTCACGTCCGTCGGTGCGATGGGCCTCGCCTCCTCGCATG
GCCTCGGCTTTGCCATCGCGTGCCCAGACCGGAAGGTCATCGTTCTTGACGGTGACGGCAGCCTGCTCATGAACCTCGGA
AGCCTGGTGACGATCGCCGCCGCGGCACCGGCCAACCTCCTGCACTTCGTTTTCGAGAACGGCACATACGAAGCCAATGG
CGGCCATCCCATTCCAGGTCAGGGCCGCGTGGATTTCGCGGGCATGGCGCGCTCTGCAGGCTACGCCGCCGTCTACCGCT
TCGAGGATCTCGGCACTTTCGAGACCGGAATAAAAGAGGTTTTGCAACAACCGGGTCCAGTCTTCGCCGACCTCAAGGTC
GTACCGGGCGCGGACAGCCCGCGCGATTATGCGGAACTCTACGATCCGGGAAAGCGCGACGCTCTGCGCGCCGAGTTGCG
GCGCGGTTAG

Upstream 100 bases:

>100_bases
AACTCTGATGCGGATGTTGCGCGTGTGGCGCCCACCCTGCGGGCCGCCTATGCGCAATCCCATCCCGTAGCCATCATGAC
CGGCAGGAGCCCTTCCTGAG

Downstream 100 bases:

>100_bases
GGAGAAGAGCCAATGGAATTACAGGATTATGCGCAGCATATCGGCGGCGAAGCCGTGGCGGCGGCGGACGGCGAAACCTA
CATTACGCACAATCCCGCCA

Product: thiamine pyrophosphate enzyme-like TPP-binding

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 189; Mature: 189

Protein sequence:

>189_residues
MMKRDQVLKILARHLDREIVIGAYSTAFDWIATRPGHPLNFTSVGAMGLASSHGLGFAIACPDRKVIVLDGDGSLLMNLG
SLVTIAAAAPANLLHFVFENGTYEANGGHPIPGQGRVDFAGMARSAGYAAVYRFEDLGTFETGIKEVLQQPGPVFADLKV
VPGADSPRDYAELYDPGKRDALRAELRRG

Sequences:

>Translated_189_residues
MMKRDQVLKILARHLDREIVIGAYSTAFDWIATRPGHPLNFTSVGAMGLASSHGLGFAIACPDRKVIVLDGDGSLLMNLG
SLVTIAAAAPANLLHFVFENGTYEANGGHPIPGQGRVDFAGMARSAGYAAVYRFEDLGTFETGIKEVLQQPGPVFADLKV
VPGADSPRDYAELYDPGKRDALRAELRRG
>Mature_189_residues
MMKRDQVLKILARHLDREIVIGAYSTAFDWIATRPGHPLNFTSVGAMGLASSHGLGFAIACPDRKVIVLDGDGSLLMNLG
SLVTIAAAAPANLLHFVFENGTYEANGGHPIPGQGRVDFAGMARSAGYAAVYRFEDLGTFETGIKEVLQQPGPVFADLKV
VPGADSPRDYAELYDPGKRDALRAELRRG

Specific function: Catalyzes the decarboxylation of sulfopyruvic acid to sulfoacetaldehyde [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022494
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 20255; Mature: 20255

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKRDQVLKILARHLDREIVIGAYSTAFDWIATRPGHPLNFTSVGAMGLASSHGLGFAIA
CCCHHHHHHHHHHHCCCEEEEEEHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCEEEEE
CPDRKVIVLDGDGSLLMNLGSLVTIAAAAPANLLHFVFENGTYEANGGHPIPGQGRVDFA
CCCCEEEEEECCCCEEHHHHHHHHHHHCCHHHHHHHHHCCCEEECCCCCCCCCCCCEEHH
GMARSAGYAAVYRFEDLGTFETGIKEVLQQPGPVFADLKVVPGADSPRDYAELYDPGKRD
HHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHCCCCCHH
ALRAELRRG
HHHHHHHCC
>Mature Secondary Structure
MMKRDQVLKILARHLDREIVIGAYSTAFDWIATRPGHPLNFTSVGAMGLASSHGLGFAIA
CCCHHHHHHHHHHHCCCEEEEEEHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCEEEEE
CPDRKVIVLDGDGSLLMNLGSLVTIAAAAPANLLHFVFENGTYEANGGHPIPGQGRVDFA
CCCCEEEEEECCCCEEHHHHHHHHHHHCCHHHHHHHHHCCCEEECCCCCCCCCCCCEEHH
GMARSAGYAAVYRFEDLGTFETGIKEVLQQPGPVFADLKVVPGADSPRDYAELYDPGKRD
HHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHCCCCCHH
ALRAELRRG
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; Decarboxylation [C]

Inhibitor: 2-oxobutanoate; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]