| Definition | Mesorhizobium sp. BNC1, complete genome. |
|---|---|
| Accession | NC_008254 |
| Length | 4,412,446 |
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The map label for this gene is capD [H]
Identifier: 110633136
GI number: 110633136
Start: 855169
End: 857103
Strand: Reverse
Name: capD [H]
Synonym: Meso_0779
Alternate gene names: 110633136
Gene position: 857103-855169 (Counterclockwise)
Preceding gene: 110633139
Following gene: 110633135
Centisome position: 19.42
GC content: 61.19
Gene sequence:
>1935_bases ATGCGATTGAGAAGCCTCGTCGAGTGGCTGCACATGCGCTCGCGCCGGACCAAAAGAGCCATGCTGCTGTCTGCAGACGT GGTTGGCCTGATGGCCATCATCTGGCTTGCCTTCTGCTTCCGTTTCAACCAGTTCTTCATTCCCAATCTCGAGCAGACGC TTTTGATCCTGGCTGCGCCGGTCATTGCCATCCCCATCTTCATCCGGATGGGCCTTTACCGGGCGGTTCTGCGGTACCTG CAGGAACAGGCGATATGGACAATGATCCGGGCGGTGACCTTCTCCAGCCTGGCATGGGTTGGGCTCGCCTTCATTACCTT ATCCTACGGCGCGGACGGCGTTCCGCGCACGATCGCTGTCCTCTATTGGGCTGGAAGTCTTGTCAGCGTGGTCGGCAGCC GCCTCGGCATCAAATGGCTGCTCAACGGGCGCATCTCTCATGCCGCGCATAAAAAGCGGGTCCTCGTTTATGGCGCTGGG GATGCGGCGATGCAGCTGGCGGAAGCGCTCAGCGCCTCCGGTGACCGTCAGGTCGTCGGCATCGTCAGCGATGATTCCGG CCTGCATGGGATGGAGATCGTCGGCATCCGGGTCTATCCCGCCGAACAGCTCGAAGACCTGGTTCTCGACACGGAGGCGC AGGAGGTCATCATCGCGGCCTCATCGTCGAGCACGCGCCGACGCCGCGAGCTTGTGACGCGGCTGGGCCGGTTCCCGGTG AAAATCCGCGTCCTTCCCTCCATCGCCGACGTGGCCGCGGGGAAATATCTCGTAAGTTATGTCCGCGACATCGATATCGA CGACCTTCTGGGCCGCTCACCGGTGCCCGCCGATCCCGAATTGATGCGGTCGAGCATCGATGAGCGCATCATAATGGTGA CTGGTGCTGCGGGATCGATCGGCTCAGAGGTGTGCCGCACAATCGCACGCGGGAAGCCGACGAAACTCGTGCTTTTCGAG ATGAACGAGCCCGGCCTTTATCAGATCGAGCGGGAGCTGCGGCGATACGGCGCTTTTCCGATCGTGCCCGTGCTCGGTTC GGTCTGCGATGCGCACCTCGTCCGGCAGACGCTGGCCGAACACGGAGTACAGACCGTCTACCACTGCGCTGCCTACAAGC ACGTGCCTCTCGTCGAATGCAATCCACTCGAGGGCGTGCGCAACAACGTATTCGGCACCTTGACCCTTGTGGAAGAAGCC TGCGCGGCCGATGTGCGAAACTTCATTCTCATCTCCTCGGACAAGGCGGTGCGGCCCACAAGCGTAATGGGCGCCACCAA GCGCCGGGCGGAGCTGATCGTGCGCTATGTCGGCGAGGAGGCCTGCAAGAAGGAGAAGAACCGCGTCTTCTCTTCCGTGC GTTTCGGAAATGTCATCGGGTCCAGTGGCTCCGTCGTACCGCTCTTCAAAGAGCAGATCGCAGCGGGAGGACCAGTCACG CTCACGCATGACGACATGACACGTTATTTCATGTCGGTGCGCGAGGCTGCGGGGCTGATCGTGCAGGCGGGCGCGCTTTC GCGCAGCGGCGATATTCTCCTCCTCGATATGGGAGAGCCGATCAGGATCCGCCAGCTTGCCGAGGATATGATCATGCTCG CCGGCTTCACCGTTCGAAACGAAGACAATCCCGATGGAGACATCGAGATCGTCACGATCGGGATGCGTGACGGGGAGAAG CTTTACGAGGAGCTTTTCTACGATCCCGCCGGCGTGACCACGACCCGCCACCCCAAGATCCTGCGGGCCGGCCAGCTTGG AACAACCGAACAGATACCGGCAATGATCGCACAGCTTAAACTCGCCGTCGAACATGGCGATGTGACCGCGGTGCGTCAGG TTCTCTTTGGGCTTATCGGCGAAAATGGCCGGCTGGCGCCTGTTCCGAAACTGCCGCCCGTGCCTACGGATGAAAACTTA CGGCGCAGGGCGTGA
Upstream 100 bases:
>100_bases ACGGTGAAGGGATTTGCTGCTAGGAGAAAAGCTTTAAAGAATAGAGGTAAGCTTTGATTTATCTGTTTATAAACGTTGTG AGATTGACCGAGGAAACAGC
Downstream 100 bases:
>100_bases GCGAGGATCAGAAAGCCGCTTTGTCGAACCCCATGCCGACGCTGTCGCAGAAGCAGCGGGCCCGGTGCATCAAGCGCTCG CTCGATCTGGCGATCGCATT
Product: polysaccharide biosynthesis protein CapD
Products: UDPglucoseal [C]
Alternate protein names: NA
Number of amino acids: Translated: 644; Mature: 644
Protein sequence:
>644_residues MRLRSLVEWLHMRSRRTKRAMLLSADVVGLMAIIWLAFCFRFNQFFIPNLEQTLLILAAPVIAIPIFIRMGLYRAVLRYL QEQAIWTMIRAVTFSSLAWVGLAFITLSYGADGVPRTIAVLYWAGSLVSVVGSRLGIKWLLNGRISHAAHKKRVLVYGAG DAAMQLAEALSASGDRQVVGIVSDDSGLHGMEIVGIRVYPAEQLEDLVLDTEAQEVIIAASSSSTRRRRELVTRLGRFPV KIRVLPSIADVAAGKYLVSYVRDIDIDDLLGRSPVPADPELMRSSIDERIIMVTGAAGSIGSEVCRTIARGKPTKLVLFE MNEPGLYQIERELRRYGAFPIVPVLGSVCDAHLVRQTLAEHGVQTVYHCAAYKHVPLVECNPLEGVRNNVFGTLTLVEEA CAADVRNFILISSDKAVRPTSVMGATKRRAELIVRYVGEEACKKEKNRVFSSVRFGNVIGSSGSVVPLFKEQIAAGGPVT LTHDDMTRYFMSVREAAGLIVQAGALSRSGDILLLDMGEPIRIRQLAEDMIMLAGFTVRNEDNPDGDIEIVTIGMRDGEK LYEELFYDPAGVTTTRHPKILRAGQLGTTEQIPAMIAQLKLAVEHGDVTAVRQVLFGLIGENGRLAPVPKLPPVPTDENL RRRA
Sequences:
>Translated_644_residues MRLRSLVEWLHMRSRRTKRAMLLSADVVGLMAIIWLAFCFRFNQFFIPNLEQTLLILAAPVIAIPIFIRMGLYRAVLRYL QEQAIWTMIRAVTFSSLAWVGLAFITLSYGADGVPRTIAVLYWAGSLVSVVGSRLGIKWLLNGRISHAAHKKRVLVYGAG DAAMQLAEALSASGDRQVVGIVSDDSGLHGMEIVGIRVYPAEQLEDLVLDTEAQEVIIAASSSSTRRRRELVTRLGRFPV KIRVLPSIADVAAGKYLVSYVRDIDIDDLLGRSPVPADPELMRSSIDERIIMVTGAAGSIGSEVCRTIARGKPTKLVLFE MNEPGLYQIERELRRYGAFPIVPVLGSVCDAHLVRQTLAEHGVQTVYHCAAYKHVPLVECNPLEGVRNNVFGTLTLVEEA CAADVRNFILISSDKAVRPTSVMGATKRRAELIVRYVGEEACKKEKNRVFSSVRFGNVIGSSGSVVPLFKEQIAAGGPVT LTHDDMTRYFMSVREAAGLIVQAGALSRSGDILLLDMGEPIRIRQLAEDMIMLAGFTVRNEDNPDGDIEIVTIGMRDGEK LYEELFYDPAGVTTTRHPKILRAGQLGTTEQIPAMIAQLKLAVEHGDVTAVRQVLFGLIGENGRLAPVPKLPPVPTDENL RRRA >Mature_644_residues MRLRSLVEWLHMRSRRTKRAMLLSADVVGLMAIIWLAFCFRFNQFFIPNLEQTLLILAAPVIAIPIFIRMGLYRAVLRYL QEQAIWTMIRAVTFSSLAWVGLAFITLSYGADGVPRTIAVLYWAGSLVSVVGSRLGIKWLLNGRISHAAHKKRVLVYGAG DAAMQLAEALSASGDRQVVGIVSDDSGLHGMEIVGIRVYPAEQLEDLVLDTEAQEVIIAASSSSTRRRRELVTRLGRFPV KIRVLPSIADVAAGKYLVSYVRDIDIDDLLGRSPVPADPELMRSSIDERIIMVTGAAGSIGSEVCRTIARGKPTKLVLFE MNEPGLYQIERELRRYGAFPIVPVLGSVCDAHLVRQTLAEHGVQTVYHCAAYKHVPLVECNPLEGVRNNVFGTLTLVEEA CAADVRNFILISSDKAVRPTSVMGATKRRAELIVRYVGEEACKKEKNRVFSSVRFGNVIGSSGSVVPLFKEQIAAGGPVT LTHDDMTRYFMSVREAAGLIVQAGALSRSGDILLLDMGEPIRIRQLAEDMIMLAGFTVRNEDNPDGDIEIVTIGMRDGEK LYEELFYDPAGVTTTRHPKILRAGQLGTTEQIPAMIAQLKLAVEHGDVTAVRQVLFGLIGENGRLAPVPKLPPVPTDENL RRRA
Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]
COG id: COG1086
COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases
Gene ontology:
Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the polysaccharide synthase family [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR016040 - InterPro: IPR003869 [H]
Pfam domain/function: PF02719 Polysacc_synt_2 [H]
EC number: 5.1.3.2 [C]
Molecular weight: Translated: 70847; Mature: 70847
Theoretical pI: Translated: 8.77; Mature: 8.77
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.1 %Cys (Translated Protein) 3.0 %Met (Translated Protein) 4.0 %Cys+Met (Translated Protein) 1.1 %Cys (Mature Protein) 3.0 %Met (Mature Protein) 4.0 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MRLRSLVEWLHMRSRRTKRAMLLSADVVGLMAIIWLAFCFRFNQFFIPNLEQTLLILAAP CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH VIAIPIFIRMGLYRAVLRYLQEQAIWTMIRAVTFSSLAWVGLAFITLSYGADGVPRTIAV HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH LYWAGSLVSVVGSRLGIKWLLNGRISHAAHKKRVLVYGAGDAAMQLAEALSASGDRQVVG HHHHHHHHHHHHHHCCEEEEECCCHHHHHCCCEEEEEECCHHHHHHHHHHHCCCCCEEEE IVSDDSGLHGMEIVGIRVYPAEQLEDLVLDTEAQEVIIAASSSSTRRRRELVTRLGRFPV EEECCCCCCCEEEEEEEECCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCE KIRVLPSIADVAAGKYLVSYVRDIDIDDLLGRSPVPADPELMRSSIDERIIMVTGAAGSI EEEECCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHCCCCEEEEEECCCCCH GSEVCRTIARGKPTKLVLFEMNEPGLYQIERELRRYGAFPIVPVLGSVCDAHLVRQTLAE HHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH HGVQTVYHCAAYKHVPLVECNPLEGVRNNVFGTLTLVEEACAADVRNFILISSDKAVRPT HHHHHHHHHHHHCCCCEEECCCCCHHHHCHHHHHHHHHHHHHHHHHCEEEECCCCCCCCH SVMGATKRRAELIVRYVGEEACKKEKNRVFSSVRFGNVIGSSGSVVPLFKEQIAAGGPVT HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCEECCCCCEEHHHHHHHCCCCCEE LTHDDMTRYFMSVREAAGLIVQAGALSRSGDILLLDMGEPIRIRQLAEDMIMLAGFTVRN EEHHHHHHHHHHHHHHHCEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCEEEEC EDNPDGDIEIVTIGMRDGEKLYEELFYDPAGVTTTRHPKILRAGQLGTTEQIPAMIAQLK CCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHH LAVEHGDVTAVRQVLFGLIGENGRLAPVPKLPPVPTDENLRRRA HHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCC >Mature Secondary Structure MRLRSLVEWLHMRSRRTKRAMLLSADVVGLMAIIWLAFCFRFNQFFIPNLEQTLLILAAP CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH VIAIPIFIRMGLYRAVLRYLQEQAIWTMIRAVTFSSLAWVGLAFITLSYGADGVPRTIAV HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH LYWAGSLVSVVGSRLGIKWLLNGRISHAAHKKRVLVYGAGDAAMQLAEALSASGDRQVVG HHHHHHHHHHHHHHCCEEEEECCCHHHHHCCCEEEEEECCHHHHHHHHHHHCCCCCEEEE IVSDDSGLHGMEIVGIRVYPAEQLEDLVLDTEAQEVIIAASSSSTRRRRELVTRLGRFPV EEECCCCCCCEEEEEEEECCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCE KIRVLPSIADVAAGKYLVSYVRDIDIDDLLGRSPVPADPELMRSSIDERIIMVTGAAGSI EEEECCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHCCCCEEEEEECCCCCH GSEVCRTIARGKPTKLVLFEMNEPGLYQIERELRRYGAFPIVPVLGSVCDAHLVRQTLAE HHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH HGVQTVYHCAAYKHVPLVECNPLEGVRNNVFGTLTLVEEACAADVRNFILISSDKAVRPT HHHHHHHHHHHHCCCCEEECCCCCHHHHCHHHHHHHHHHHHHHHHHCEEEECCCCCCCCH SVMGATKRRAELIVRYVGEEACKKEKNRVFSSVRFGNVIGSSGSVVPLFKEQIAAGGPVT HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCEECCCCCEEHHHHHHHCCCCCEE LTHDDMTRYFMSVREAAGLIVQAGALSRSGDILLLDMGEPIRIRQLAEDMIMLAGFTVRN EEHHHHHHHHHHHHHHHCEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCEEEEC EDNPDGDIEIVTIGMRDGEKLYEELFYDPAGVTTTRHPKILRAGQLGTTEQIPAMIAQLK CCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHH LAVEHGDVTAVRQVLFGLIGENGRLAPVPKLPPVPTDENLRRRA HHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NAD+ [C]
Metal ions: NA
Kcat value (1/min): 57600 [C]
Specific activity: 233.3
Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]
Substrates: UDPglucose [C]
Specific reaction: UDPglucose <==> UDPglucoseal [C]
General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 7961465 [H]