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The map label for this gene is trpA [H]

Identifier: 110633021

GI number: 110633021

Start: 743114

End: 743959

Strand: Reverse

Name: trpA [H]

Synonym: Meso_0664

Alternate gene names: 110633021

Gene position: 743959-743114 (Counterclockwise)

Preceding gene: 110633022

Following gene: 110633020

Centisome position: 16.86

GC content: 63.59

Gene sequence:

>846_bases
ATGACCACCACCCGAATTGACAGGCTCTTCACCAAGCTGAAAGCCGAGAACAGGCCAGCGCTCGTCACCTATTTCATGGG
TGGCGACCCCGATTACGAAACGTCGCTCTCCATTATGAAAGCCCTGCCGGTGGCCGGCAGCGACATCATCGAGCTCGGCA
TGCCCTTTTCCGACCCGATGGCCGACGGGCCGGCGATCCAGGCGGCGGGGCTGCGCGCGTTGAAAGGCGGACAGACACTG
GACAAGACGCTTCGGATGGCGAAGAGCTTCCGGCAGGATAACGACCATACGCCGATCGTCATGATGGGCTACTACAATCC
CATCTACATCTACGGTGTAGACAGGTTCCTCACGGAGGCGAGAGCGTCCGGCATCGACGGGCTCATCATCGTCGACCTGC
CGCCGGAGATGGACGAAGAATTGTGCGTGCCCGCGTTGAGGGCCGGCATCAACTTCATCCGGCTAGCAACGCCAACAACG
GACGACAAGCGCCTGCCCAAGGTGCTCGAAAACACGTCCGGATTCGTCTATTACGTGTCGATGACCGGCATCACCGGCTC
GGCGCTGCCGGACACCTCCAAGGTGGCTGGCGCCGTCGCCCGCATCAAGGGGCATACCAAACTGCCGGTCTGCGTCGGCT
TTGGCGTAAAGACGGCGAGCCAGGCGCGTGCGATCGGAGCGGCCGCGGACGGCGTGGTGGTCGGCACGGCTATCGTGAAT
GCGGTTGCGAATGTGCTCGGCCCCGACGGCAAAACAACTGCCGACCCGGCGGAAGCGGTGGCAACCCTGGTGCGCGGCCT
TGCCGATGGCGTGCGCGAGGCGCGCATCACGGCCGCCGCCGAATAA

Upstream 100 bases:

>100_bases
CCCTGCACCCTTATGAAGTTCCGTCGATCACGGCGACACAGATGTGCAATATCGACCAAGCCTATGCCGACTGGCTGCGC
GCGGAAACGAGGGAGCCGGC

Downstream 100 bases:

>100_bases
CGGACAATTTCTCCCGGAATTATATCGGAGAGGAAGGAGCATCTGCCATGAACTGGATTACCAACTACGTCCGGCCGAAG
ATCAACTCCATGCTCGGCCG

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MTTTRIDRLFTKLKAENRPALVTYFMGGDPDYETSLSIMKALPVAGSDIIELGMPFSDPMADGPAIQAAGLRALKGGQTL
DKTLRMAKSFRQDNDHTPIVMMGYYNPIYIYGVDRFLTEARASGIDGLIIVDLPPEMDEELCVPALRAGINFIRLATPTT
DDKRLPKVLENTSGFVYYVSMTGITGSALPDTSKVAGAVARIKGHTKLPVCVGFGVKTASQARAIGAAADGVVVGTAIVN
AVANVLGPDGKTTADPAEAVATLVRGLADGVREARITAAAE

Sequences:

>Translated_281_residues
MTTTRIDRLFTKLKAENRPALVTYFMGGDPDYETSLSIMKALPVAGSDIIELGMPFSDPMADGPAIQAAGLRALKGGQTL
DKTLRMAKSFRQDNDHTPIVMMGYYNPIYIYGVDRFLTEARASGIDGLIIVDLPPEMDEELCVPALRAGINFIRLATPTT
DDKRLPKVLENTSGFVYYVSMTGITGSALPDTSKVAGAVARIKGHTKLPVCVGFGVKTASQARAIGAAADGVVVGTAIVN
AVANVLGPDGKTTADPAEAVATLVRGLADGVREARITAAAE
>Mature_280_residues
TTTRIDRLFTKLKAENRPALVTYFMGGDPDYETSLSIMKALPVAGSDIIELGMPFSDPMADGPAIQAAGLRALKGGQTLD
KTLRMAKSFRQDNDHTPIVMMGYYNPIYIYGVDRFLTEARASGIDGLIIVDLPPEMDEELCVPALRAGINFIRLATPTTD
DKRLPKVLENTSGFVYYVSMTGITGSALPDTSKVAGAVARIKGHTKLPVCVGFGVKTASQARAIGAAADGVVVGTAIVNA
VANVLGPDGKTTADPAEAVATLVRGLADGVREARITAAAE

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate [H]

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family [H]

Homologues:

Organism=Escherichia coli, GI1787514, Length=219, Percent_Identity=34.2465753424658, Blast_Score=135, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6321412, Length=265, Percent_Identity=40.377358490566, Blast_Score=190, Evalue=2e-49,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028 [H]

Pfam domain/function: PF00290 Trp_syntA [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 29617; Mature: 29486

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTRIDRLFTKLKAENRPALVTYFMGGDPDYETSLSIMKALPVAGSDIIELGMPFSDPM
CCCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCHHEECCCCCCCC
ADGPAIQAAGLRALKGGQTLDKTLRMAKSFRQDNDHTPIVMMGYYNPIYIYGVDRFLTEA
CCCCCCHHHCHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEHHHHHHHH
RASGIDGLIIVDLPPEMDEELCVPALRAGINFIRLATPTTDDKRLPKVLENTSGFVYYVS
HHCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEE
MTGITGSALPDTSKVAGAVARIKGHTKLPVCVGFGVKTASQARAIGAAADGVVVGTAIVN
EECCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHCCCCCCHHHHHHHHH
AVANVLGPDGKTTADPAEAVATLVRGLADGVREARITAAAE
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCC
>Mature Secondary Structure 
TTTRIDRLFTKLKAENRPALVTYFMGGDPDYETSLSIMKALPVAGSDIIELGMPFSDPM
CCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCHHEECCCCCCCC
ADGPAIQAAGLRALKGGQTLDKTLRMAKSFRQDNDHTPIVMMGYYNPIYIYGVDRFLTEA
CCCCCCHHHCHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEHHHHHHHH
RASGIDGLIIVDLPPEMDEELCVPALRAGINFIRLATPTTDDKRLPKVLENTSGFVYYVS
HHCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEE
MTGITGSALPDTSKVAGAVARIKGHTKLPVCVGFGVKTASQARAIGAAADGVVVGTAIVN
EECCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHCCCCCCHHHHHHHHH
AVANVLGPDGKTTADPAEAVATLVRGLADGVREARITAAAE
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]