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The map label for this gene is yjbJ [H]

Identifier: 110632877

GI number: 110632877

Start: 573691

End: 574455

Strand: Reverse

Name: yjbJ [H]

Synonym: Meso_0517

Alternate gene names: 110632877

Gene position: 574455-573691 (Counterclockwise)

Preceding gene: 110632878

Following gene: 110632876

Centisome position: 13.02

GC content: 69.15

Gene sequence:

>765_bases
ATGCGCGTCTCCCATCGCCAGCCCGCTGCCGGAGCGAAAGGGGCCGCCCGCCGATTCCCCTTCATTCTCCTTTCCGGCCT
GGCCTTTGCCGGCGCGATGCCCGCGCCGTCTCTGGCGCAGACCTCGCTCATCGAGCGACCCGCTGCAGGCTCGCCCTACA
CACAAGCGGTCGCCGAGGCAGCGCAGCGGTTCGGCATTCCCGAGCGCTGGATCTGGGCAGTCATGCGCGTCGAGAGCGGC
GGTCGTGTCCGCGCGGTCTCGCCCAAAGGCGCGATGGGACTCATGCAGATCATGCCCGCGACCTGGGCGGATCTGCGGGC
GCGCCACAGTCTGGGGGCCGATCCCTTTGACCCTCGCGACAACATCCTCGCTGGCGCAGCCTATCTGCGCGAAATGCACG
ACCGCTACGGCGCGCCGGGCTTTCTCGCGGCCTACAATGCCGGCCCCGGGCGGTACGATGACTATCTCTCGGCTGGCCGC
GCGCTGCCCGCCGAGACCATCGCCTACGTGGCCGCGATCGCGCCGTTGATCGGCAGTGAGCCGCTCGCTGCCGGCGTCGC
TGTCGCGACCATCGACCCGCTCGCGTGGACTCGGTCTCCGTTGTTCGTCGCGCGCTCCGAAGGCAGCGTTGCAGCAGAAC
GATCGCAGCCCGATCGCCAAAGGAACGGCAGCCGAACCGCACATCCGCAGCAACCCCCAATTGCCGGTTCGACGCAGACG
GCAGGCATCTTCGTGACCCGCGCCGATGATGGGGGGCGTCCATGA

Upstream 100 bases:

>100_bases
ACAGCCGATCCGGGCCAACCTGTTCCAGTCGGCCGACGACAAGTCAGCATGGGGTCTCTACTGGAACCGGCCGACCAAGC
GCAGCGAGCGGGACTGACCG

Downstream 100 bases:

>100_bases
CCGGAATCGCACTCCGGCGCGGTGTAGCGAGCTCCCGGAAAGAGGCGGCACGGGGCCGTTGGGGCGAAGCAGACACGACC
AACAGATGGCGAGATAAAAG

Product: lytic transglycosylase, catalytic

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MRVSHRQPAAGAKGAARRFPFILLSGLAFAGAMPAPSLAQTSLIERPAAGSPYTQAVAEAAQRFGIPERWIWAVMRVESG
GRVRAVSPKGAMGLMQIMPATWADLRARHSLGADPFDPRDNILAGAAYLREMHDRYGAPGFLAAYNAGPGRYDDYLSAGR
ALPAETIAYVAAIAPLIGSEPLAAGVAVATIDPLAWTRSPLFVARSEGSVAAERSQPDRQRNGSRTAHPQQPPIAGSTQT
AGIFVTRADDGGRP

Sequences:

>Translated_254_residues
MRVSHRQPAAGAKGAARRFPFILLSGLAFAGAMPAPSLAQTSLIERPAAGSPYTQAVAEAAQRFGIPERWIWAVMRVESG
GRVRAVSPKGAMGLMQIMPATWADLRARHSLGADPFDPRDNILAGAAYLREMHDRYGAPGFLAAYNAGPGRYDDYLSAGR
ALPAETIAYVAAIAPLIGSEPLAAGVAVATIDPLAWTRSPLFVARSEGSVAAERSQPDRQRNGSRTAHPQQPPIAGSTQT
AGIFVTRADDGGRP
>Mature_254_residues
MRVSHRQPAAGAKGAARRFPFILLSGLAFAGAMPAPSLAQTSLIERPAAGSPYTQAVAEAAQRFGIPERWIWAVMRVESG
GRVRAVSPKGAMGLMQIMPATWADLRARHSLGADPFDPRDNILAGAAYLREMHDRYGAPGFLAAYNAGPGRYDDYLSAGR
ALPAETIAYVAAIAPLIGSEPLAAGVAVATIDPLAWTRSPLFVARSEGSVAAERSQPDRQRNGSRTAHPQQPPIAGSTQT
AGIFVTRADDGGRP

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=122, Percent_Identity=36.8852459016393, Blast_Score=60, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 26781; Mature: 26781

Theoretical pI: Translated: 10.52; Mature: 10.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSHRQPAAGAKGAARRFPFILLSGLAFAGAMPAPSLAQTSLIERPAAGSPYTQAVAEA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHH
AQRFGIPERWIWAVMRVESGGRVRAVSPKGAMGLMQIMPATWADLRARHSLGADPFDPRD
HHHCCCCHHHHHHHEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
NILAGAAYLREMHDRYGAPGFLAAYNAGPGRYDDYLSAGRALPAETIAYVAAIAPLIGSE
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
PLAAGVAVATIDPLAWTRSPLFVARSEGSVAAERSQPDRQRNGSRTAHPQQPPIAGSTQT
CHHHCEEEEEECCHHHCCCCEEEEECCCCEECCCCCCHHHHCCCCCCCCCCCCCCCCCCC
AGIFVTRADDGGRP
CEEEEEECCCCCCC
>Mature Secondary Structure
MRVSHRQPAAGAKGAARRFPFILLSGLAFAGAMPAPSLAQTSLIERPAAGSPYTQAVAEA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHH
AQRFGIPERWIWAVMRVESGGRVRAVSPKGAMGLMQIMPATWADLRARHSLGADPFDPRD
HHHCCCCHHHHHHHEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
NILAGAAYLREMHDRYGAPGFLAAYNAGPGRYDDYLSAGRALPAETIAYVAAIAPLIGSE
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
PLAAGVAVATIDPLAWTRSPLFVARSEGSVAAERSQPDRQRNGSRTAHPQQPPIAGSTQT
CHHHCEEEEEECCHHHCCCCEEEEECCCCEECCCCCCHHHHCCCCCCCCCCCCCCCCCCC
AGIFVTRADDGGRP
CEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]