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The map label for this gene is pit [C]

Identifier: 109948218

GI number: 109948218

Start: 1526322

End: 1527923

Strand: Reverse

Name: pit [C]

Synonym: Hac_1751

Alternate gene names: 109948218

Gene position: 1527923-1526322 (Counterclockwise)

Preceding gene: 109948227

Following gene: 109948217

Centisome position: 98.33

GC content: 42.88

Gene sequence:

>1602_bases
ATGGAAATTAAAAATATCAAAGAGTTTGAAAAAGCTTCTAAAAAACTCCAAAAAGACACTTTAAAAATCGCTTTTGCACT
TTTGTTTCTCATTGTTGCCGCTTTGCTCGCTCTTATTTTTGGGCAGACTAACTCCAGGAGCTTGTTGCTCATGTTTGCGG
CTGTGATTGGGGGGTATATGGCGATGAATATTGGCGCTAATGATGTGTGCAATAATGTCGGTCCTGCCGTAGGATCTAAA
GCCATTAGCATGGGAGGAGCGATTTTGATCGCTGGGGTTTGTGAAATGCTTGGGGCGGTTATCGCTGGGGGGGAAGTGGT
TTCTACCATTAAAGGCCGTATCGTTTTGCCTGAATTGATTGCAGATGCACATATTTTCATTAAAGTGATGTTGGCAAGCT
TACTCAGTGGGGCGTTGTGGTTGCATGTAGCCACTTTAATTGGTGCACCAGTTTCTACTTCGCATTCTGTGGTAGGGGGG
GTGATTGGTGCTGGAATAGCGGCCGCTGGTGCGTCTGTGGTCAATTGGAGTTTTTTACTAGGCATTGTGGCTAGCTGGGT
GGTTTCGCCTTTAATGGGGGCTTTGATAGCCATGTTTTTTTTAATGTTTATCAAAAAGACCATCGCCTATAAAGAAGATA
AAAAGAGTGCGGCTTTAAAAGTCGTGCCTTACTTGGTGGCGTTGATGATCTTATCGTTTAGTTGGTATTTAGTGTTAAAA
GTCTTCAAACGCCTCTACGCGGTGGGTTTTGAAATCCAGCTTGTTTGTGGCTGTATCCTTGCACTTTTAATCTTTATTCT
TTTTAAAAGATTTGCACTAAAAAAAGCCCTAAAGTTAGAAAATAGCCATGAGAGCATCAATGAGCTTTTTAATATCCCTT
TGATTTTTGCTGCTGCACTTTTGAGCTTTGCGCATGGGGCTAATGATGTGGCTAATGCGATTGGCCCGCTAGCAGCGATC
AGTCAGACTTTAGGGGAGGCGAGCGGTTCTGTAAAGAATGCTTTAAGTTCTGTGCCGTTGTGGATTATGATCATAGGGGG
GGCTGGGATTGCTTTAGGCTTGAGCTTGTATGGGCCAAAACTCATCAAAACGGTGGGCTCTGAAATCACAGAGTTGGATA
AAATGCAAGCTTTTTGCATTGCGCTTTCTGCGGTTATCACCGTGCTTTTAGCCTCTCAATTGGGCTTACCGGTAAGCTCT
ACGCACATTGTGGTAGGGGCGGTGTTTGGAGTGGGCTTTTTAAGAGAGCGCTTAAGAGAGCAATCAAGAAGGCGTTTTGC
CAAGATTAGAGACGACATCGTAGCGGCACACTTTGGGGGGGATTTAGAAGAAATTGAAGGCTTTTTGGAGCGCTTTGATA
AAGCCAGTTTGAAAGAAAAATCGCTCATGTTAGAGAGCTTGAAAAAAAGCAAGAATACCGCCATTGCTTTGGAATTGAAA
AAGAAAGAGAAAAAGTCGCTCAAAAAAGTGTATAAAGAAGAAGTGATCAAACGCTCCATTTTAAAAAAGATTGTCACCGC
TTGGCTTGTCACGGTGCCCATTTCTGCGTTATTAGGAGTGCTTCTTTTTATCGTCCTTAATTTTGCGGAAAAATTTTTCT
AG

Upstream 100 bases:

>100_bases
ACATAATGAGCCATTTTGATCAAACAACTCTTTTTAAGAGTGGCTCAAGGGCTAATTGGTTAGAATACGCCCATTGTTAT
AACAAGGTTTTGATGACGAT

Downstream 100 bases:

>100_bases
GGTTTTAAAGGCCTGATTTTAAAGTTAGGCTTAATCTTTTATGTTAAAATTGTAAGATTTTAGTTATCGTTAGGTTTTTT
AAAATCATGGGGAGGATCAA

Product: phosphate permease

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MEIKNIKEFEKASKKLQKDTLKIAFALLFLIVAALLALIFGQTNSRSLLLMFAAVIGGYMAMNIGANDVCNNVGPAVGSK
AISMGGAILIAGVCEMLGAVIAGGEVVSTIKGRIVLPELIADAHIFIKVMLASLLSGALWLHVATLIGAPVSTSHSVVGG
VIGAGIAAAGASVVNWSFLLGIVASWVVSPLMGALIAMFFLMFIKKTIAYKEDKKSAALKVVPYLVALMILSFSWYLVLK
VFKRLYAVGFEIQLVCGCILALLIFILFKRFALKKALKLENSHESINELFNIPLIFAAALLSFAHGANDVANAIGPLAAI
SQTLGEASGSVKNALSSVPLWIMIIGGAGIALGLSLYGPKLIKTVGSEITELDKMQAFCIALSAVITVLLASQLGLPVSS
THIVVGAVFGVGFLRERLREQSRRRFAKIRDDIVAAHFGGDLEEIEGFLERFDKASLKEKSLMLESLKKSKNTAIALELK
KKEKKSLKKVYKEEVIKRSILKKIVTAWLVTVPISALLGVLLFIVLNFAEKFF

Sequences:

>Translated_533_residues
MEIKNIKEFEKASKKLQKDTLKIAFALLFLIVAALLALIFGQTNSRSLLLMFAAVIGGYMAMNIGANDVCNNVGPAVGSK
AISMGGAILIAGVCEMLGAVIAGGEVVSTIKGRIVLPELIADAHIFIKVMLASLLSGALWLHVATLIGAPVSTSHSVVGG
VIGAGIAAAGASVVNWSFLLGIVASWVVSPLMGALIAMFFLMFIKKTIAYKEDKKSAALKVVPYLVALMILSFSWYLVLK
VFKRLYAVGFEIQLVCGCILALLIFILFKRFALKKALKLENSHESINELFNIPLIFAAALLSFAHGANDVANAIGPLAAI
SQTLGEASGSVKNALSSVPLWIMIIGGAGIALGLSLYGPKLIKTVGSEITELDKMQAFCIALSAVITVLLASQLGLPVSS
THIVVGAVFGVGFLRERLREQSRRRFAKIRDDIVAAHFGGDLEEIEGFLERFDKASLKEKSLMLESLKKSKNTAIALELK
KKEKKSLKKVYKEEVIKRSILKKIVTAWLVTVPISALLGVLLFIVLNFAEKFF
>Mature_533_residues
MEIKNIKEFEKASKKLQKDTLKIAFALLFLIVAALLALIFGQTNSRSLLLMFAAVIGGYMAMNIGANDVCNNVGPAVGSK
AISMGGAILIAGVCEMLGAVIAGGEVVSTIKGRIVLPELIADAHIFIKVMLASLLSGALWLHVATLIGAPVSTSHSVVGG
VIGAGIAAAGASVVNWSFLLGIVASWVVSPLMGALIAMFFLMFIKKTIAYKEDKKSAALKVVPYLVALMILSFSWYLVLK
VFKRLYAVGFEIQLVCGCILALLIFILFKRFALKKALKLENSHESINELFNIPLIFAAALLSFAHGANDVANAIGPLAAI
SQTLGEASGSVKNALSSVPLWIMIIGGAGIALGLSLYGPKLIKTVGSEITELDKMQAFCIALSAVITVLLASQLGLPVSS
THIVVGAVFGVGFLRERLREQSRRRFAKIRDDIVAAHFGGDLEEIEGFLERFDKASLKEKSLMLESLKKSKNTAIALELK
KKEKKSLKKVYKEEVIKRSILKKIVTAWLVTVPISALLGVLLFIVLNFAEKFF

Specific function: Potential transporter for phosphate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=138, Percent_Identity=39.8550724637681, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI5803173, Length=132, Percent_Identity=40.9090909090909, Blast_Score=107, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71981576, Length=429, Percent_Identity=31.2354312354312, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17557328, Length=498, Percent_Identity=25.5020080321285, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI25146401, Length=140, Percent_Identity=41.4285714285714, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17539280, Length=273, Percent_Identity=30.03663003663, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI32566716, Length=180, Percent_Identity=32.2222222222222, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17536725, Length=157, Percent_Identity=27.3885350318471, Blast_Score=79, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6319773, Length=136, Percent_Identity=38.9705882352941, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI21356511, Length=241, Percent_Identity=32.7800829875519, Blast_Score=111, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 57177; Mature: 57177

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9252185 [H]