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The map label for this gene is pnp

Identifier: 109948073

GI number: 109948073

Start: 1383708

End: 1385774

Strand: Reverse

Name: pnp

Synonym: Hac_1591

Alternate gene names: 109948073

Gene position: 1385774-1383708 (Counterclockwise)

Preceding gene: 109948074

Following gene: 109948072

Centisome position: 89.18

GC content: 39.43

Gene sequence:

>2067_bases
ATGGATTTTATCACCATCAATTCTATTAACAAAACCGAAGAATTCGCCCTCAAACAAGTGGCCAAGCAAGCCACTAGCTC
TCTTTTATACCGATTAGGAAAAACCATCATTTTAGCGAGCGTGTGCGTAGAAAGAGAGCTTGTGAGCGAAGATTTTTTGC
CTTTAGTGGTGCAGTTTTTAGAAAAATCTTATGCTGCCGGTAAGATTCCTGGCGGTTTTGTTAAACGAGAAGGCAGAGCG
CAAGATTTTGAAATCTTAACTTCCAGGCTCATAGATAGGACTTTACGCCCTTTATTCCCTAAAGACTACCGCTACCCCAC
GCAAATCACTTTAATGGTTTTAAGTCATGATATTGAAAATGACTTGCAAGTCTCTGCTTTAAATGCCGCTTCAGCCGCTC
TTTTTTTAGCCCATGCAGCTCCTTTTAAAAGCGTGAGCGCTTGTAGGATCGCTAGGATTGATAACGAATTTATTATCAAC
CCAAGCACAAGCCTTTTGAATCAATCCAGTTTGGACTTATTCGTGTCCGGCACTAAAGAGAGTTTGAACATGATAGAAAT
GCGCTCTTTGGGGCAAAAATTAAACGCTTTAGAAGAGCCTTTAATGCTAGAAGCTTTAGAATTAGCCCAAAAAAGTTTAA
AAGAAACTTGCACGCTTTATGAAGAAACTTTCACGCCCCATCAAAACGCGCTTCTTTTTAAAGAGAGCCAAGGAATAGTC
TTTAATGAAAGGCTATTAGATTTATTAAAAAATCAGTATTTTAATGAAATCATTAAGGGTATTGAAAGCTCTGCTTTGAG
CGAGCGAGAAAATGTTTTCAATGAAATTGCCAGAAAAATCAGTGAAGCCCACTCAGAATTTAATTTAGAAGAAATTGAAT
TGTCTTTAGAAAAAGTGAAAAAAACCGAGATTAGGCGCATGATTATTAGGGATAAAATCCGCCCAGACAAACGCGCGTTA
GAAGAAGTTAGACCCATTTCTATAGAGAGCAATTTGCTCCCTATGGCGCATAGCTCTATTTTATTCACTAGGGGGCAGAC
TCAAAGCTTGGTCGTAGGGGTTTTAGGCACGGATAATGACGCTCAAACCCATGAAAGTTTGGAGCATAAAGCCCCTATAA
AAGAACGCTTCATGTTCCATTATAATTTCCCTCCTTTTTGCGTGGGTGAAGCGAGTTCTATTGGCGCAACTTCAAGGCGT
GAATTAGGGCATGGGAATTTGGCCAAAAGAGCCTTAGAAACAAGCATTAAAAATAAAGATCAGGTGATACGATTGGTTTC
TGAGATTTTAGAAAGCAATGGATCAAGCTCAATGGCGAGCGTGTGTGCAGGCTCTTTAGCCCTTTATGCAAGCGGTGTAG
AAATCCATGATTTAGTCGCTGGGGTGGCTATGGGCATGGTGAGTGAAGGGCAAGATTACGCCATTTTAAGCGATATTAGC
GGATTAGAAGATGCAGAAGGCGATATGGACTTTAAGATTGCTGGGAATTTAGAAGGCATTACAGCCATGCAAATGGATAC
CAAAATGAGCGGTATTCAATTAGAAATCTTATACCAAACCTTACTCCAGGCTAAAAGGGTGCGAGAACATATTTTAAAAA
TCATGCATGAAGCGAAAGAAAAGATTGTGATCAATTTTTCTCATTTGCCCACAACTGAAATTTTTAATGTCGCACCGGAT
AAAATTATAGAAATTATCGGTCAAGGGGGGCGTGTGATTAGAGAAATAGTAGAAAAGTTTGAAGTTAAAATTGATTTGAA
TAAACCGAGCGGTGAAGTGAAAATCATGGGGAATAAAGAGCGTGTTTTAAAAACTAAAGAATTTATTTTAAATTATTTGC
ATTCTTTAGATCAAGAATTAGAGCAATACGCTATTGATGAGGTGCTAGAAGCTCAAGTGAAACGAATCGTGGATTTTGGG
GCGTTTTTGAGCCTACCTAAGGGGGGTGAAGGCTTGTTGAGAAAGCAACACATGGAGAGGTGTCAAGTGGCTTTAAAAGA
AGGCGATAGCATTCGGTGTAGGGTGATTAGCTTCAATAAGGGTAAAATCGCCTTGGATTTGGCTTAA

Upstream 100 bases:

>100_bases
TTGAGCATCATTATAAAGAACTCAAGCGATTGAGCAATGAAGAAATTGAAAAATACTTGGGCACTAACAACGCACCCAAT
TTAAAAAAGGAACATTAAAT

Downstream 100 bases:

>100_bases
AATTTAAAAAAGCGTTTTTAAAAGAGATTTTAAGCTAGTTTGTATTAAAATGAATTTCTGCTTGTGTTTTTGAAAGTAGA
GGAACGATTCGAAATAAAAA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 688; Mature: 688

Protein sequence:

>688_residues
MDFITINSINKTEEFALKQVAKQATSSLLYRLGKTIILASVCVERELVSEDFLPLVVQFLEKSYAAGKIPGGFVKREGRA
QDFEILTSRLIDRTLRPLFPKDYRYPTQITLMVLSHDIENDLQVSALNAASAALFLAHAAPFKSVSACRIARIDNEFIIN
PSTSLLNQSSLDLFVSGTKESLNMIEMRSLGQKLNALEEPLMLEALELAQKSLKETCTLYEETFTPHQNALLFKESQGIV
FNERLLDLLKNQYFNEIIKGIESSALSERENVFNEIARKISEAHSEFNLEEIELSLEKVKKTEIRRMIIRDKIRPDKRAL
EEVRPISIESNLLPMAHSSILFTRGQTQSLVVGVLGTDNDAQTHESLEHKAPIKERFMFHYNFPPFCVGEASSIGATSRR
ELGHGNLAKRALETSIKNKDQVIRLVSEILESNGSSSMASVCAGSLALYASGVEIHDLVAGVAMGMVSEGQDYAILSDIS
GLEDAEGDMDFKIAGNLEGITAMQMDTKMSGIQLEILYQTLLQAKRVREHILKIMHEAKEKIVINFSHLPTTEIFNVAPD
KIIEIIGQGGRVIREIVEKFEVKIDLNKPSGEVKIMGNKERVLKTKEFILNYLHSLDQELEQYAIDEVLEAQVKRIVDFG
AFLSLPKGGEGLLRKQHMERCQVALKEGDSIRCRVISFNKGKIALDLA

Sequences:

>Translated_688_residues
MDFITINSINKTEEFALKQVAKQATSSLLYRLGKTIILASVCVERELVSEDFLPLVVQFLEKSYAAGKIPGGFVKREGRA
QDFEILTSRLIDRTLRPLFPKDYRYPTQITLMVLSHDIENDLQVSALNAASAALFLAHAAPFKSVSACRIARIDNEFIIN
PSTSLLNQSSLDLFVSGTKESLNMIEMRSLGQKLNALEEPLMLEALELAQKSLKETCTLYEETFTPHQNALLFKESQGIV
FNERLLDLLKNQYFNEIIKGIESSALSERENVFNEIARKISEAHSEFNLEEIELSLEKVKKTEIRRMIIRDKIRPDKRAL
EEVRPISIESNLLPMAHSSILFTRGQTQSLVVGVLGTDNDAQTHESLEHKAPIKERFMFHYNFPPFCVGEASSIGATSRR
ELGHGNLAKRALETSIKNKDQVIRLVSEILESNGSSSMASVCAGSLALYASGVEIHDLVAGVAMGMVSEGQDYAILSDIS
GLEDAEGDMDFKIAGNLEGITAMQMDTKMSGIQLEILYQTLLQAKRVREHILKIMHEAKEKIVINFSHLPTTEIFNVAPD
KIIEIIGQGGRVIREIVEKFEVKIDLNKPSGEVKIMGNKERVLKTKEFILNYLHSLDQELEQYAIDEVLEAQVKRIVDFG
AFLSLPKGGEGLLRKQHMERCQVALKEGDSIRCRVISFNKGKIALDLA
>Mature_688_residues
MDFITINSINKTEEFALKQVAKQATSSLLYRLGKTIILASVCVERELVSEDFLPLVVQFLEKSYAAGKIPGGFVKREGRA
QDFEILTSRLIDRTLRPLFPKDYRYPTQITLMVLSHDIENDLQVSALNAASAALFLAHAAPFKSVSACRIARIDNEFIIN
PSTSLLNQSSLDLFVSGTKESLNMIEMRSLGQKLNALEEPLMLEALELAQKSLKETCTLYEETFTPHQNALLFKESQGIV
FNERLLDLLKNQYFNEIIKGIESSALSERENVFNEIARKISEAHSEFNLEEIELSLEKVKKTEIRRMIIRDKIRPDKRAL
EEVRPISIESNLLPMAHSSILFTRGQTQSLVVGVLGTDNDAQTHESLEHKAPIKERFMFHYNFPPFCVGEASSIGATSRR
ELGHGNLAKRALETSIKNKDQVIRLVSEILESNGSSSMASVCAGSLALYASGVEIHDLVAGVAMGMVSEGQDYAILSDIS
GLEDAEGDMDFKIAGNLEGITAMQMDTKMSGIQLEILYQTLLQAKRVREHILKIMHEAKEKIVINFSHLPTTEIFNVAPD
KIIEIIGQGGRVIREIVEKFEVKIDLNKPSGEVKIMGNKERVLKTKEFILNYLHSLDQELEQYAIDEVLEAQVKRIVDFG
AFLSLPKGGEGLLRKQHMERCQVALKEGDSIRCRVISFNKGKIALDLA

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=663, Percent_Identity=33.3333333333333, Blast_Score=302, Evalue=7e-82,
Organism=Escherichia coli, GI145693187, Length=691, Percent_Identity=35.89001447178, Blast_Score=392, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI115534063, Length=648, Percent_Identity=31.1728395061728, Blast_Score=267, Evalue=1e-71,
Organism=Drosophila melanogaster, GI281362905, Length=591, Percent_Identity=35.5329949238579, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24651641, Length=591, Percent_Identity=35.5329949238579, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24651643, Length=591, Percent_Identity=35.5329949238579, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI161079377, Length=542, Percent_Identity=36.3468634686347, Blast_Score=286, Evalue=4e-77,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_HELAH (Q17VM4)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_665301.1
- ProteinModelPortal:   Q17VM4
- STRING:   Q17VM4
- GeneID:   4176785
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_1591
- NMPDR:   fig|382638.8.peg.1539
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- BioCyc:   HACI382638:HAC_1591-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 77045; Mature: 77045

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFITINSINKTEEFALKQVAKQATSSLLYRLGKTIILASVCVERELVSEDFLPLVVQFL
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
EKSYAAGKIPGGFVKREGRAQDFEILTSRLIDRTLRPLFPKDYRYPTQITLMVLSHDIEN
HHHCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCC
DLQVSALNAASAALFLAHAAPFKSVSACRIARIDNEFIINPSTSLLNQSSLDLFVSGTKE
CCEEEHHHHHHHHHHHHHCCCCCCHHHHHHEECCCCEEECCCHHHHCCCCCEEEEECCHH
SLNMIEMRSLGQKLNALEEPLMLEALELAQKSLKETCTLYEETFTPHQNALLFKESQGIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCE
FNERLLDLLKNQYFNEIIKGIESSALSERENVFNEIARKISEAHSEFNLEEIELSLEKVK
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
KTEIRRMIIRDKIRPDKRALEEVRPISIESNLLPMAHSSILFTRGQTQSLVVGVLGTDND
HHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEEECCCC
AQTHESLEHKAPIKERFMFHYNFPPFCVGEASSIGATSRRELGHGNLAKRALETSIKNKD
HHHHHHHHHCCCHHHCEEEEECCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCHH
QVIRLVSEILESNGSSSMASVCAGSLALYASGVEIHDLVAGVAMGMVSEGQDYAILSDIS
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEHHCC
GLEDAEGDMDFKIAGNLEGITAMQMDTKMSGIQLEILYQTLLQAKRVREHILKIMHEAKE
CCCCCCCCCCEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIVINFSHLPTTEIFNVAPDKIIEIIGQGGRVIREIVEKFEVKIDLNKPSGEVKIMGNKE
EEEEEECCCCCHHHHCCCHHHHHHHHCCCCHHHHHHHHHHEEEEEECCCCCCEEEECCHH
RVLKTKEFILNYLHSLDQELEQYAIDEVLEAQVKRIVDFGAFLSLPKGGEGLLRKQHMER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
CQVALKEGDSIRCRVISFNKGKIALDLA
HHHHHHCCCCEEEEEEEECCCEEEEEEC
>Mature Secondary Structure
MDFITINSINKTEEFALKQVAKQATSSLLYRLGKTIILASVCVERELVSEDFLPLVVQFL
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
EKSYAAGKIPGGFVKREGRAQDFEILTSRLIDRTLRPLFPKDYRYPTQITLMVLSHDIEN
HHHCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCC
DLQVSALNAASAALFLAHAAPFKSVSACRIARIDNEFIINPSTSLLNQSSLDLFVSGTKE
CCEEEHHHHHHHHHHHHHCCCCCCHHHHHHEECCCCEEECCCHHHHCCCCCEEEEECCHH
SLNMIEMRSLGQKLNALEEPLMLEALELAQKSLKETCTLYEETFTPHQNALLFKESQGIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCE
FNERLLDLLKNQYFNEIIKGIESSALSERENVFNEIARKISEAHSEFNLEEIELSLEKVK
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
KTEIRRMIIRDKIRPDKRALEEVRPISIESNLLPMAHSSILFTRGQTQSLVVGVLGTDND
HHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEEECCCC
AQTHESLEHKAPIKERFMFHYNFPPFCVGEASSIGATSRRELGHGNLAKRALETSIKNKD
HHHHHHHHHCCCHHHCEEEEECCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCHH
QVIRLVSEILESNGSSSMASVCAGSLALYASGVEIHDLVAGVAMGMVSEGQDYAILSDIS
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEHHCC
GLEDAEGDMDFKIAGNLEGITAMQMDTKMSGIQLEILYQTLLQAKRVREHILKIMHEAKE
CCCCCCCCCCEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIVINFSHLPTTEIFNVAPDKIIEIIGQGGRVIREIVEKFEVKIDLNKPSGEVKIMGNKE
EEEEEECCCCCHHHHCCCHHHHHHHHCCCCHHHHHHHHHHEEEEEECCCCCCEEEECCHH
RVLKTKEFILNYLHSLDQELEQYAIDEVLEAQVKRIVDFGAFLSLPKGGEGLLRKQHMER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
CQVALKEGDSIRCRVISFNKGKIALDLA
HHHHHHCCCCEEEEEEEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA